Click here to switch to the map view.

The map label for this gene is smeIM [H]

Identifier: 75675042

GI number: 75675042

Start: 924490

End: 925248

Strand: Direct

Name: smeIM [H]

Synonym: Nwi_0847

Alternate gene names: 75675042

Gene position: 924490-925248 (Clockwise)

Preceding gene: 75675041

Following gene: 75675043

Centisome position: 27.17

GC content: 66.93

Gene sequence:

>759_bases
ATGACGGTCACCGACTTTTCCGTGATTACGGGCGATTGTCGCGTCCTGATGCCGGCGCAGGGTCCGTTCGACCTGATCCT
AGCCGATCCGCCCTATGGCGACACGTCGCTCGCCTGGGATCGCCGTGTCGACGGTTGGCTGCAGCTTGCATGCGCCGCGT
TGAAGCCATCGGGATCGCTCTGGGTGTTCGGCTCGCTGCGCAGCTTCATGGCGACGGCCGATCAATTTGCCGACGCCGAC
CTGCGCTACGCGCAGGAGATCGTGTGGGAGAAGCAGAACGGCACCAGCTTCCACGCCGATCGCTTCAAGCGTGTGCATGA
GCTGGTCGCGCAGTTCTATCGCGCGGACGTGCCGTGGAGCGCCGTTTATAACGACGTGCAGACGACGCCCGACGCGACGG
CGCGCACCGTGCGGCGCAAGATGCCACCGCCGCATACCGGCCGCATCGACGCCGGCCACTACGTCAGCGAGGACGGCGGA
CCGCGGCTGATGCGCTCCGTGATCTACGCGCGCAATTGCCACGGCACGGCAATCCATCCGACGGAAAAGCCGTCCGGCCT
GATCGAAATCCTGATCCGCACGAGCTGCCCGCAAGGGGGCCTGGTCGGCGATTGGTTCGTCGGATCGGGCGCCGCCGGCG
AAGCCTGCCGGCTCGCCGGGCGGCGCTATGTCGGCTGCGAGATCGACGCCGGCATGGCCGAGCTTGCGCGGGGGCGCATC
ACATCGGTGCTGCCGTTCATCGAGGGAGCGCAGCCATAA

Upstream 100 bases:

>100_bases
TGGGAACGCCGACTCCCGTTCTATTAGGAACGCGAGTCCCGTACTATTGGGAACGCGCGCGCGCCGTATCGTCCGGCCGC
CAAGCGTCGGAGATCGCGTC

Downstream 100 bases:

>100_bases
CGGCAGGGCTGCGCGCCATCGGTCAGCCGGGCGACCTTACGCTTGTCGAACTGACGTGGCGCGAGAAGCGCATCGAGCAC
CGTATCCGCTTCGGCCGGAT

Product: DNA methylase N-4/N-6

Products: NA

Alternate protein names: M.SmeI; Adenine-specific methyltransferase SmeIP; M.CcrMI ortholog; Modification methylase SmeIP; M.SmeIP [H]

Number of amino acids: Translated: 252; Mature: 251

Protein sequence:

>252_residues
MTVTDFSVITGDCRVLMPAQGPFDLILADPPYGDTSLAWDRRVDGWLQLACAALKPSGSLWVFGSLRSFMATADQFADAD
LRYAQEIVWEKQNGTSFHADRFKRVHELVAQFYRADVPWSAVYNDVQTTPDATARTVRRKMPPPHTGRIDAGHYVSEDGG
PRLMRSVIYARNCHGTAIHPTEKPSGLIEILIRTSCPQGGLVGDWFVGSGAAGEACRLAGRRYVGCEIDAGMAELARGRI
TSVLPFIEGAQP

Sequences:

>Translated_252_residues
MTVTDFSVITGDCRVLMPAQGPFDLILADPPYGDTSLAWDRRVDGWLQLACAALKPSGSLWVFGSLRSFMATADQFADAD
LRYAQEIVWEKQNGTSFHADRFKRVHELVAQFYRADVPWSAVYNDVQTTPDATARTVRRKMPPPHTGRIDAGHYVSEDGG
PRLMRSVIYARNCHGTAIHPTEKPSGLIEILIRTSCPQGGLVGDWFVGSGAAGEACRLAGRRYVGCEIDAGMAELARGRI
TSVLPFIEGAQP
>Mature_251_residues
TVTDFSVITGDCRVLMPAQGPFDLILADPPYGDTSLAWDRRVDGWLQLACAALKPSGSLWVFGSLRSFMATADQFADADL
RYAQEIVWEKQNGTSFHADRFKRVHELVAQFYRADVPWSAVYNDVQTTPDATARTVRRKMPPPHTGRIDAGHYVSEDGGP
RLMRSVIYARNCHGTAIHPTEKPSGLIEILIRTSCPQGGLVGDWFVGSGAAGEACRLAGRRYVGCEIDAGMAELARGRIT
SVLPFIEGAQP

Specific function: This methylase recognizes the double-stranded sequence GANTC and causes specific methylation on A-2 on both strands. Ccrm-mediated methylation has important cellular functions. Appears to contribute to the accurate cell-cycle control of DNA replication an

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR002052
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 27586; Mature: 27454

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVTDFSVITGDCRVLMPAQGPFDLILADPPYGDTSLAWDRRVDGWLQLACAALKPSGSL
CCCCCCEEEECCCEEEEECCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCE
WVFGSLRSFMATADQFADADLRYAQEIVWEKQNGTSFHADRFKRVHELVAQFYRADVPWS
EEHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHH
AVYNDVQTTPDATARTVRRKMPPPHTGRIDAGHYVSEDGGPRLMRSVIYARNCHGTAIHP
HHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCEECCCCCHHHHHHHHHHHCCCCCCCCC
TEKPSGLIEILIRTSCPQGGLVGDWFVGSGAAGEACRLAGRRYVGCEIDAGMAELARGRI
CCCCCHHEEEEEECCCCCCCCEECCCCCCCCCCHHHHHCCCEEEEEECCCCHHHHHHHHH
TSVLPFIEGAQP
HHHHHHHCCCCC
>Mature Secondary Structure 
TVTDFSVITGDCRVLMPAQGPFDLILADPPYGDTSLAWDRRVDGWLQLACAALKPSGSL
CCCCCEEEECCCEEEEECCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCE
WVFGSLRSFMATADQFADADLRYAQEIVWEKQNGTSFHADRFKRVHELVAQFYRADVPWS
EEHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHH
AVYNDVQTTPDATARTVRRKMPPPHTGRIDAGHYVSEDGGPRLMRSVIYARNCHGTAIHP
HHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCEECCCCCHHHHHHHHHHHCCCCCCCCC
TEKPSGLIEILIRTSCPQGGLVGDWFVGSGAAGEACRLAGRRYVGCEIDAGMAELARGRI
CCCCCHHEEEEEECCCCCCCCEECCCCCCCCCCHHHHHCCCEEEEEECCCCHHHHHHHHH
TSVLPFIEGAQP
HHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9294447; 11481430 [H]