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The map label for this gene is braD [H]

Identifier: 75674862

GI number: 75674862

Start: 746258

End: 747151

Strand: Direct

Name: braD [H]

Synonym: Nwi_0665

Alternate gene names: 75674862

Gene position: 746258-747151 (Clockwise)

Preceding gene: 75674860

Following gene: 75674863

Centisome position: 21.94

GC content: 58.39

Gene sequence:

>894_bases
TTGAGTCTCGAATTATTTCTGACGGTGGCACTCAACGGTCTTACCGTCTCCGCACTCTACTTCATCGTGGCGATCGGATT
CTCGCTCATCTTCGGTCTGATGCGCTCCGTGAACATGGCGCACGGAACGCTGTTTCTTGTCGGCGCGTATGCCGGCGTCG
CCGTTTTCTATCCGCTTTACGACGACGGCAACGCTTATGCCTGGATCATCGCGTTGGCCGCAGGAATTTCCGCATCGGCG
ATTCTTGGTGTCGTTTTGCAGATTGTCTTTCTTGGCTGGATGCAGGGTCAGGAGCTGCGCCAGACGCTGGTGACGATCGG
AATCTCCATAATCGTCGCGGATCAACTCCTGGCGATTTTTGGCGGACTGAGTCAGCAGTTCTATGCGCCCGACATTCTGT
TTGGGTCGGTGCCGGTGCCATTCGTTCAAGGCGGTCAGTACTCGACCTTCCGTCTGTTTCAGGTGGTGGTGGCGATCGGT
GTCGGCTTCGGTCTTTGGCTGATCCTGCAACGGACAAAGCTCGGCATCGTGGTGCGCGCCGGTGTCGATGACCGGGCGAT
GCTGGCGGCGCAAGGCGTCAACGTGCCGCTCATCTCAGTTGTCATTTTTGCGATCGGCGCCGCTCTTGCCGGCCTGGCCG
GGGTCATAGGTGGCACCGCGCAGCCCTTCGGCCAGGAGAAGGATATCGAGTTCCTGCTGACATCACTTGTCGTGGTTATC
GTGGGCGGCATGGGATCGTTACCCGGGACGGCGCTCGGCGCACTTCTGGTCGGAATGGCCGAGCAGTTCGGACAATCGTT
GTTCCCGGCTTACTCGGTGATGCTCACATTGCTCATCATGGTAGCGGTCCTCGCGTTCCGGCCTCACGGTCTTCTGGGAA
GAAAACTATCATGA

Upstream 100 bases:

>100_bases
GCAATGCGCAAAATACGTAAAATAAATCTGCTTCTCATTTGCTATAGAATCTTCACGCGTTGAAAGAGCGCGATGTATCC
GGAATGGTGGAGCGCGTGAC

Downstream 100 bases:

>100_bases
GTGACGCGGCTTCCACATCTACTACGGAGGTCGTCGGACGCTCCTTTGCTTTGTCGTCCCTGATCACTTCCGCTGTTCTG
ATAGCGCTTGTTACGCTTCC

Product: branched chain amino acid ABC transporter permease

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MSLELFLTVALNGLTVSALYFIVAIGFSLIFGLMRSVNMAHGTLFLVGAYAGVAVFYPLYDDGNAYAWIIALAAGISASA
ILGVVLQIVFLGWMQGQELRQTLVTIGISIIVADQLLAIFGGLSQQFYAPDILFGSVPVPFVQGGQYSTFRLFQVVVAIG
VGFGLWLILQRTKLGIVVRAGVDDRAMLAAQGVNVPLISVVIFAIGAALAGLAGVIGGTAQPFGQEKDIEFLLTSLVVVI
VGGMGSLPGTALGALLVGMAEQFGQSLFPAYSVMLTLLIMVAVLAFRPHGLLGRKLS

Sequences:

>Translated_297_residues
MSLELFLTVALNGLTVSALYFIVAIGFSLIFGLMRSVNMAHGTLFLVGAYAGVAVFYPLYDDGNAYAWIIALAAGISASA
ILGVVLQIVFLGWMQGQELRQTLVTIGISIIVADQLLAIFGGLSQQFYAPDILFGSVPVPFVQGGQYSTFRLFQVVVAIG
VGFGLWLILQRTKLGIVVRAGVDDRAMLAAQGVNVPLISVVIFAIGAALAGLAGVIGGTAQPFGQEKDIEFLLTSLVVVI
VGGMGSLPGTALGALLVGMAEQFGQSLFPAYSVMLTLLIMVAVLAFRPHGLLGRKLS
>Mature_296_residues
SLELFLTVALNGLTVSALYFIVAIGFSLIFGLMRSVNMAHGTLFLVGAYAGVAVFYPLYDDGNAYAWIIALAAGISASAI
LGVVLQIVFLGWMQGQELRQTLVTIGISIIVADQLLAIFGGLSQQFYAPDILFGSVPVPFVQGGQYSTFRLFQVVVAIGV
GFGLWLILQRTKLGIVVRAGVDDRAMLAAQGVNVPLISVVIFAIGAALAGLAGVIGGTAQPFGQEKDIEFLLTSLVVVIV
GGMGSLPGTALGALLVGMAEQFGQSLFPAYSVMLTLLIMVAVLAFRPHGLLGRKLS

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0559

COG function: function code E; Branched-chain amino acid ABC-type transport system, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789866, Length=303, Percent_Identity=24.4224422442244, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851 [H]

Pfam domain/function: PF02653 BPD_transp_2 [H]

EC number: NA

Molecular weight: Translated: 31115; Mature: 30983

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLELFLTVALNGLTVSALYFIVAIGFSLIFGLMRSVNMAHGTLFLVGAYAGVAVFYPLY
CCEEEEEEEHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHCHHHHHHHHHHHHHEEEEEE
DDGNAYAWIIALAAGISASAILGVVLQIVFLGWMQGQELRQTLVTIGISIIVADQLLAIF
ECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
GGLSQQFYAPDILFGSVPVPFVQGGQYSTFRLFQVVVAIGVGFGLWLILQRTKLGIVVRA
CCCCHHHCCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEC
GVDDRAMLAAQGVNVPLISVVIFAIGAALAGLAGVIGGTAQPFGQEKDIEFLLTSLVVVI
CCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
VGGMGSLPGTALGALLVGMAEQFGQSLFPAYSVMLTLLIMVAVLAFRPHGLLGRKLS
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SLELFLTVALNGLTVSALYFIVAIGFSLIFGLMRSVNMAHGTLFLVGAYAGVAVFYPLY
CEEEEEEEHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHCHHHHHHHHHHHHHEEEEEE
DDGNAYAWIIALAAGISASAILGVVLQIVFLGWMQGQELRQTLVTIGISIIVADQLLAIF
ECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
GGLSQQFYAPDILFGSVPVPFVQGGQYSTFRLFQVVVAIGVGFGLWLILQRTKLGIVVRA
CCCCHHHCCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEC
GVDDRAMLAAQGVNVPLISVVIFAIGAALAGLAGVIGGTAQPFGQEKDIEFLLTSLVVVI
CCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
VGGMGSLPGTALGALLVGMAEQFGQSLFPAYSVMLTLLIMVAVLAFRPHGLLGRKLS
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043; 2509433 [H]