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The map label for this gene is thcD [H]

Identifier: 75674768

GI number: 75674768

Start: 644871

End: 646391

Strand: Direct

Name: thcD [H]

Synonym: Nwi_0570

Alternate gene names: 75674768

Gene position: 644871-646391 (Clockwise)

Preceding gene: 75674766

Following gene: 75674769

Centisome position: 18.96

GC content: 64.69

Gene sequence:

>1521_bases
ATGGCCCAGGAGCAGGCTCAACCGAGCGGACCAGACCTGACGCAGGGCGTCGATCCCGGCGATTTCAAGGATGGGAAGCT
GGTCGGCCATGTCGGCGACCAGGAGATCCTGTTGGTCCGCGAGGGCGAGGGGATTTTCGCGATCGACGCTCATTGCAGCC
ATTACCACGGACCGCTTGCTGACGGGATCGTTGTCGACGGCACCGTCCGCTGTCCATGGCATCATGCCTGCTTCGATCTC
CGCACCGGGGAAGCGACCTGCGCGCCGGCCTTCAACGCGCTCGATGTCTGGCAGGTCGAGCAACGTGACGGCCGGATTTT
TGTTCGCGAGAAGCGAGCGCGGCCGGCGGTCAGGATCGCGAAGCCTGATCACCCCGGCAAGATCGTCATCGTCGGCGGCG
GCGCGGCGGGTTTTGCGGCCGCTGAGATGCTGCGCCGTCAGGACTACGCAGGCAGCATCGTGATGCTGAGCAGCGACGAT
GCGCCGCCGGTCGACCGGCCGAACCTGTCGAAGGACTACCTCGCCGGTTCCGCGCCGGAAGACTGGCTCCCGCTGCGTCC
CGATGATTTCTACCAGCAGGCCGGCATCGATCTGCGGCTGCGCACGACCGTTACCGCCATCGATCCGTCCGCGCGCCAGC
TTTCGATCAACGGCGATGTCATTGGCTACGATCGGCTGCTTCTGGCGACCGGAGCCGAGCCGGTCAGGCTGCCGATTCCC
GGCGCCAATCTTTCGCATGTCCATACGCTGCGCTCGCTCGCGGATTGCCGCGCCATCATTGATGGAGCCAAATCGGCCAA
CCGGGCGGTTGTCATCGGTGCGAGTTTCATCGGCCTGGAGGTCGCGGCCTCGTTGCGCGCCCGCGGGATCGAGGTCCATG
TCGTCGCCCCTGAGTCCCGTCCGATGGAGCGCATCCTCGGCGCGGAAATGGGCGATTTCGTCCGTTCGCTGCACGAGGAA
CATGGCGTGATCTTCCACCTGGAGAATACAGTCACAGCGATCGGCGAGAAGAAGGTGACAATCAGCAGCGGCGAAACGCT
GGAAGCCGATTTCGTCGTCTTCGGCGTCGGCGTGAAGCCGCGCCTGGAGCTTGCGGAGAAGGCGGGATTGAAGATCGACC
GCGGCGTGCTCGTCAACCAGTACCTGGAAACAAGCGCGCCCGGAATCTTTGCGGCCGGTGACATCGCGCGCTGGCCCGAT
CCGCGGTTCGGGGAGAACATCCGGGTCGAGCACTGGGTGGTGGCGCAGCGCCAGGGGCAGGTGGCCGCGCGCAACATGCT
GGGACACCGCGAAAAGTATGACGCTGTGCCGTTCTTCTGGAGCCAGCATTATGATGTGCCGATCAATTATGTCGGCCACG
CGTTGAAGTGGGACCAGATCGAGGTCGAGGGTAGTGTCGCTGACCGGGATTGCCTGCTGCGCTACAAGCATCTGGGGCGC
GTGCTGGCGGTCTCTTCGATCTACCGGGATGTCGACCACCTCAAGGCGGAAGTGGCGATGGAGCGCGCGGTCGCCGCCTG
A

Upstream 100 bases:

>100_bases
ATGTGACCTTGCCGGACCGACCTCGGTGTGAAACCGTGCGGCGTAAGAACGCGATTGTGGCTCGCGGATTCCGCGCCGCG
CAACCATGGAGGACCGATAG

Downstream 100 bases:

>100_bases
CATTCAGCCTCGCGGTATACCCACCCGCCGGAGCAGGCGTGATCACGGGGTTTTCTCAAGGCGCGACCCAACGCAGGAAT
GCGCTGTGGCGTTTTCGAGC

Product: FAD-dependent pyridine nucleotide-disulphide oxidoreductase

Products: Oxidized Ferredoxin; NADH; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 506; Mature: 505

Protein sequence:

>506_residues
MAQEQAQPSGPDLTQGVDPGDFKDGKLVGHVGDQEILLVREGEGIFAIDAHCSHYHGPLADGIVVDGTVRCPWHHACFDL
RTGEATCAPAFNALDVWQVEQRDGRIFVREKRARPAVRIAKPDHPGKIVIVGGGAAGFAAAEMLRRQDYAGSIVMLSSDD
APPVDRPNLSKDYLAGSAPEDWLPLRPDDFYQQAGIDLRLRTTVTAIDPSARQLSINGDVIGYDRLLLATGAEPVRLPIP
GANLSHVHTLRSLADCRAIIDGAKSANRAVVIGASFIGLEVAASLRARGIEVHVVAPESRPMERILGAEMGDFVRSLHEE
HGVIFHLENTVTAIGEKKVTISSGETLEADFVVFGVGVKPRLELAEKAGLKIDRGVLVNQYLETSAPGIFAAGDIARWPD
PRFGENIRVEHWVVAQRQGQVAARNMLGHREKYDAVPFFWSQHYDVPINYVGHALKWDQIEVEGSVADRDCLLRYKHLGR
VLAVSSIYRDVDHLKAEVAMERAVAA

Sequences:

>Translated_506_residues
MAQEQAQPSGPDLTQGVDPGDFKDGKLVGHVGDQEILLVREGEGIFAIDAHCSHYHGPLADGIVVDGTVRCPWHHACFDL
RTGEATCAPAFNALDVWQVEQRDGRIFVREKRARPAVRIAKPDHPGKIVIVGGGAAGFAAAEMLRRQDYAGSIVMLSSDD
APPVDRPNLSKDYLAGSAPEDWLPLRPDDFYQQAGIDLRLRTTVTAIDPSARQLSINGDVIGYDRLLLATGAEPVRLPIP
GANLSHVHTLRSLADCRAIIDGAKSANRAVVIGASFIGLEVAASLRARGIEVHVVAPESRPMERILGAEMGDFVRSLHEE
HGVIFHLENTVTAIGEKKVTISSGETLEADFVVFGVGVKPRLELAEKAGLKIDRGVLVNQYLETSAPGIFAAGDIARWPD
PRFGENIRVEHWVVAQRQGQVAARNMLGHREKYDAVPFFWSQHYDVPINYVGHALKWDQIEVEGSVADRDCLLRYKHLGR
VLAVSSIYRDVDHLKAEVAMERAVAA
>Mature_505_residues
AQEQAQPSGPDLTQGVDPGDFKDGKLVGHVGDQEILLVREGEGIFAIDAHCSHYHGPLADGIVVDGTVRCPWHHACFDLR
TGEATCAPAFNALDVWQVEQRDGRIFVREKRARPAVRIAKPDHPGKIVIVGGGAAGFAAAEMLRRQDYAGSIVMLSSDDA
PPVDRPNLSKDYLAGSAPEDWLPLRPDDFYQQAGIDLRLRTTVTAIDPSARQLSINGDVIGYDRLLLATGAEPVRLPIPG
ANLSHVHTLRSLADCRAIIDGAKSANRAVVIGASFIGLEVAASLRARGIEVHVVAPESRPMERILGAEMGDFVRSLHEEH
GVIFHLENTVTAIGEKKVTISSGETLEADFVVFGVGVKPRLELAEKAGLKIDRGVLVNQYLETSAPGIFAAGDIARWPDP
RFGENIRVEHWVVAQRQGQVAARNMLGHREKYDAVPFFWSQHYDVPINYVGHALKWDQIEVEGSVADRDCLLRYKHLGRV
LAVSSIYRDVDHLKAEVAMERAVAA

Specific function: The degradation of the thiocarbamate herbicide EPTC by cytochrome CYP116 (thcB) requires the participation of a flavoprotein, rhodocoxin reductase, and an iron-sulfur protein, rhodocoxin, to mediate the transfer of electrons from NADH to P450 for oxygen a

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI226437568, Length=492, Percent_Identity=31.5040650406504, Blast_Score=261, Evalue=1e-69,
Organism=Homo sapiens, GI65787454, Length=492, Percent_Identity=31.5040650406504, Blast_Score=261, Evalue=1e-69,
Organism=Homo sapiens, GI21389617, Length=492, Percent_Identity=31.5040650406504, Blast_Score=261, Evalue=1e-69,
Organism=Homo sapiens, GI22202629, Length=346, Percent_Identity=26.3005780346821, Blast_Score=79, Evalue=7e-15,
Organism=Homo sapiens, GI4757732, Length=346, Percent_Identity=26.3005780346821, Blast_Score=79, Evalue=8e-15,
Organism=Homo sapiens, GI22202631, Length=163, Percent_Identity=32.5153374233129, Blast_Score=67, Evalue=5e-11,
Organism=Escherichia coli, GI1788892, Length=331, Percent_Identity=33.5347432024169, Blast_Score=142, Evalue=6e-35,
Organism=Escherichia coli, GI1789065, Length=351, Percent_Identity=27.6353276353276, Blast_Score=119, Evalue=6e-28,
Organism=Escherichia coli, GI1789765, Length=321, Percent_Identity=30.2180685358255, Blast_Score=108, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI17559934, Length=437, Percent_Identity=32.4942791762014, Blast_Score=225, Evalue=4e-59,
Organism=Caenorhabditis elegans, GI32564386, Length=341, Percent_Identity=27.5659824046921, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24639257, Length=478, Percent_Identity=33.6820083682008, Blast_Score=261, Evalue=9e-70,
Organism=Drosophila melanogaster, GI281359715, Length=478, Percent_Identity=33.6820083682008, Blast_Score=261, Evalue=9e-70,
Organism=Drosophila melanogaster, GI281359713, Length=478, Percent_Identity=33.6820083682008, Blast_Score=261, Evalue=9e-70,
Organism=Drosophila melanogaster, GI24639250, Length=478, Percent_Identity=33.6820083682008, Blast_Score=261, Evalue=9e-70,
Organism=Drosophila melanogaster, GI18543267, Length=478, Percent_Identity=33.6820083682008, Blast_Score=261, Evalue=9e-70,
Organism=Drosophila melanogaster, GI24639252, Length=478, Percent_Identity=33.6820083682008, Blast_Score=261, Evalue=9e-70,
Organism=Drosophila melanogaster, GI24585130, Length=481, Percent_Identity=28.0665280665281, Blast_Score=207, Evalue=9e-54,
Organism=Drosophila melanogaster, GI28573993, Length=355, Percent_Identity=28.7323943661972, Blast_Score=122, Evalue=8e-28,
Organism=Drosophila melanogaster, GI24581020, Length=355, Percent_Identity=28.7323943661972, Blast_Score=122, Evalue=8e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3 [C]

Molecular weight: Translated: 55202; Mature: 55071

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQEQAQPSGPDLTQGVDPGDFKDGKLVGHVGDQEILLVREGEGIFAIDAHCSHYHGPLA
CCCCCCCCCCCCHHCCCCCCCCCCCEEEEECCCCEEEEEECCCCEEEEECCCCCCCCCCC
DGIVVDGTVRCPWHHACFDLRTGEATCAPAFNALDVWQVEQRDGRIFVREKRARPAVRIA
CCEEEECEEECCCCHHEEEECCCCCCCCCCCCCCEEEEEECCCCEEEEEECCCCCEEEEE
KPDHPGKIVIVGGGAAGFAAAEMLRRQDYAGSIVMLSSDDAPPVDRPNLSKDYLAGSAPE
CCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHCCCCCCC
DWLPLRPDDFYQQAGIDLRLRTTVTAIDPSARQLSINGDVIGYDRLLLATGAEPVRLPIP
CCCCCCCCHHHHHCCCCEEEEEEEEEECCCCEEEEECCCEEECCEEEEECCCCCEEEECC
GANLSHVHTLRSLADCRAIIDGAKSANRAVVIGASFIGLEVAASLRARGIEVHVVAPESR
CCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHCCEEEEEECCCCC
PMERILGAEMGDFVRSLHEEHGVIFHLENTVTAIGEKKVTISSGETLEADFVVFGVGVKP
CHHHHHCCHHHHHHHHHHHHCCEEEEECCCEEECCCEEEEECCCCEEEEEEEEEECCCCC
RLELAEKAGLKIDRGVLVNQYLETSAPGIFAAGDIARWPDPRFGENIRVEHWVVAQRQGQ
HHHHHHHCCCEECCCHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCEEEEEEEECCCCC
VAARNMLGHREKYDAVPFFWSQHYDVPINYVGHALKWDQIEVEGSVADRDCLLRYKHLGR
HHHHHHHCCCHHCCCCCEEECCCCCCCHHHCCCEEEECEEEEECCCCCHHHHHHHHHHHH
VLAVSSIYRDVDHLKAEVAMERAVAA
HHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AQEQAQPSGPDLTQGVDPGDFKDGKLVGHVGDQEILLVREGEGIFAIDAHCSHYHGPLA
CCCCCCCCCCCHHCCCCCCCCCCCEEEEECCCCEEEEEECCCCEEEEECCCCCCCCCCC
DGIVVDGTVRCPWHHACFDLRTGEATCAPAFNALDVWQVEQRDGRIFVREKRARPAVRIA
CCEEEECEEECCCCHHEEEECCCCCCCCCCCCCCEEEEEECCCCEEEEEECCCCCEEEEE
KPDHPGKIVIVGGGAAGFAAAEMLRRQDYAGSIVMLSSDDAPPVDRPNLSKDYLAGSAPE
CCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHCCCCCCC
DWLPLRPDDFYQQAGIDLRLRTTVTAIDPSARQLSINGDVIGYDRLLLATGAEPVRLPIP
CCCCCCCCHHHHHCCCCEEEEEEEEEECCCCEEEEECCCEEECCEEEEECCCCCEEEECC
GANLSHVHTLRSLADCRAIIDGAKSANRAVVIGASFIGLEVAASLRARGIEVHVVAPESR
CCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHCCEEEEEECCCCC
PMERILGAEMGDFVRSLHEEHGVIFHLENTVTAIGEKKVTISSGETLEADFVVFGVGVKP
CHHHHHCCHHHHHHHHHHHHCCEEEEECCCEEECCCEEEEECCCCEEEEEEEEEECCCCC
RLELAEKAGLKIDRGVLVNQYLETSAPGIFAAGDIARWPDPRFGENIRVEHWVVAQRQGQ
HHHHHHHCCCEECCCHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCEEEEEEEECCCCC
VAARNMLGHREKYDAVPFFWSQHYDVPINYVGHALKWDQIEVEGSVADRDCLLRYKHLGR
HHHHHHHCCCHHCCCCCEEECCCCCCCHHHCCCEEEECEEEEECCCCCHHHHHHHHHHHH
VLAVSSIYRDVDHLKAEVAMERAVAA
HHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced Ferredoxin; NAD(+); 3-phenylpropionate; NADH; Proton; O2 [C]

Specific reaction: Reduced Ferredoxin + NAD(+) = Oxidized Ferredoxin + NADH. 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]