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The map label for this gene is 75674755

Identifier: 75674755

GI number: 75674755

Start: 626435

End: 627643

Strand: Direct

Name: 75674755

Synonym: Nwi_0557

Alternate gene names: NA

Gene position: 626435-627643 (Clockwise)

Preceding gene: 75674754

Following gene: 75674758

Centisome position: 18.41

GC content: 64.35

Gene sequence:

>1209_bases
ATGGCCATGTCGGACGTGGAGAACGCGAAAGCGGAGCCGCTGGCGATCTGGACGATCGGCTTCAGACCGTTCTTCCTGAT
GGCCGGCATCTGGTCGGCGGTGGCGCTCGCCGGATGGATCGCCGTGCTCACGATCGGGCTCACGCTGCCAAGCCGCTTCG
ATCCGCTCACATGGCATATCCATGAAATGCTGTTCGGGTTCGTCCTGGCGGCGATTGCCGGTTTTCTGCTGACCGCGATT
CCGAACTGGACCGGCCGCCCGCCGATCAGCGGCCGGCTGCTCGCCGCGCTCGTGATGTTATGGTTCGCGGGTCGCGTCAC
CTGTCTCGTTTCAGCGTGGTTCCCGCTCGGGCTGGCGGCCGCGATCGATCTGGCCTTCCCGTTCGTGCTTTGCGGAGTTG
TCGCTCGCGAAATCATCGCGGGCCGCAACTGGCGCAACATGATCATGCCGATCCCTGTCGCCATGCTCGGCGTCGCCGAT
CTTCTTATGTATCTGGAGCTGTCTGGTTCTTCCCTGTCCGTGGGTCTTGGATGGCGTCTCGGACTGGCCGCGATCATCGT
TCTGATCTCGGTCGTCGGCGGCCGGATCATCCCGAGTTTCACGCGCAACTGGCTCATCAGGCAGAATGCTTCCCGCCTTC
CGCCCGCGCACGGCCACGTCGATAGCCTCGCGCTCGTGTCCCTCCTTGCCGGAATGATCGGATGGGCGCTCTTTCCTGAG
CGCTGGCCTTTCGGCTGGTTGTTGCTTGTCGCCGCCGCGCTGAATCTCTGGCGGCTCGGGCGCTGGCGGGGCCCGGCCGT
CGGCGCTGAACCGCTGTTGACTATTCTTCACGCCGGATACCTGTGGGTCATTATCGGTGCGGCATTGCTTGGCGCTTCGA
TGCTGGCCGATCATGTGCCTCAGGCCGCTGCGATCCACGCGCTGACCGCCGGTGCCATCGGCACGATGACGCTCGGCGTC
ATGACCCGCGTTTCGCTCGGCCACACCGGACGCGCCCTCACAGCGGATCGGATCACGGGCCTGATCTATCTGCTGGTGAT
CCTCGCCGCCATCGCGCGGGTCGCTGCCTGGGATGGCGGTTCGTCGGCGATGTTGATCGAGATATCAGCCGTGCTGTGGA
TCGGCAGCTTCGGCCTTTTCGTGTTGCGATACGGACCGATACTGCTGTCGCCCCGCGTGAAGTCGGTGGACATTCAGCCT
CGCCGGTAA

Upstream 100 bases:

>100_bases
CCGCGCAACCTCAGGCGCAGGCATCAAGGATACACATCCCTGACCGCGCCATCACTCCGCATTTGCTGATATGGTCGCGC
GGAACGATGAGGGATGGCGC

Downstream 100 bases:

>100_bases
CAATGCTTCCCGCGCTGCGAGTGCCCGCGCGGCGCACGCAACTCCGAATGTCCTCCAAACTAATGACTATCGGTTGATAT
TCAGTACCGCGCCCGTCGTG

Product: NnrS

Products: AMP; diphosphate; L-asparagine; L-glutamate

Alternate protein names: NnrS Protein; Heme/Copper Membrane Protein; Heme-Cu Protein NnrS; Protein Involved In Response NO; Nnrs Family Protein; NnrS Transmembrane Protein; Transmembrane Protein; NnrS Dehydrogenase; Heme-Cu-Containing Membrane Protein; Heme/Copper Membrane Protein Of NnrS Family Protein; Nitrite And/Or Nitric Oxide Reductase; NnrS-Like Protein; Asparagine Synthetase B; ATP Synthase F0 Subunit A; Heme-Cu Membrane Protein; LOW QUALITY PROTEIN NnrS Family Protein; ATP Synthase F0 A Subunit; Integral Inner Membrane Permease; NnrS Protein Superfamily; Asparagine Synthetase

Number of amino acids: Translated: 402; Mature: 401

Protein sequence:

>402_residues
MAMSDVENAKAEPLAIWTIGFRPFFLMAGIWSAVALAGWIAVLTIGLTLPSRFDPLTWHIHEMLFGFVLAAIAGFLLTAI
PNWTGRPPISGRLLAALVMLWFAGRVTCLVSAWFPLGLAAAIDLAFPFVLCGVVAREIIAGRNWRNMIMPIPVAMLGVAD
LLMYLELSGSSLSVGLGWRLGLAAIIVLISVVGGRIIPSFTRNWLIRQNASRLPPAHGHVDSLALVSLLAGMIGWALFPE
RWPFGWLLLVAAALNLWRLGRWRGPAVGAEPLLTILHAGYLWVIIGAALLGASMLADHVPQAAAIHALTAGAIGTMTLGV
MTRVSLGHTGRALTADRITGLIYLLVILAAIARVAAWDGGSSAMLIEISAVLWIGSFGLFVLRYGPILLSPRVKSVDIQP
RR

Sequences:

>Translated_402_residues
MAMSDVENAKAEPLAIWTIGFRPFFLMAGIWSAVALAGWIAVLTIGLTLPSRFDPLTWHIHEMLFGFVLAAIAGFLLTAI
PNWTGRPPISGRLLAALVMLWFAGRVTCLVSAWFPLGLAAAIDLAFPFVLCGVVAREIIAGRNWRNMIMPIPVAMLGVAD
LLMYLELSGSSLSVGLGWRLGLAAIIVLISVVGGRIIPSFTRNWLIRQNASRLPPAHGHVDSLALVSLLAGMIGWALFPE
RWPFGWLLLVAAALNLWRLGRWRGPAVGAEPLLTILHAGYLWVIIGAALLGASMLADHVPQAAAIHALTAGAIGTMTLGV
MTRVSLGHTGRALTADRITGLIYLLVILAAIARVAAWDGGSSAMLIEISAVLWIGSFGLFVLRYGPILLSPRVKSVDIQP
RR
>Mature_401_residues
AMSDVENAKAEPLAIWTIGFRPFFLMAGIWSAVALAGWIAVLTIGLTLPSRFDPLTWHIHEMLFGFVLAAIAGFLLTAIP
NWTGRPPISGRLLAALVMLWFAGRVTCLVSAWFPLGLAAAIDLAFPFVLCGVVAREIIAGRNWRNMIMPIPVAMLGVADL
LMYLELSGSSLSVGLGWRLGLAAIIVLISVVGGRIIPSFTRNWLIRQNASRLPPAHGHVDSLALVSLLAGMIGWALFPER
WPFGWLLLVAAALNLWRLGRWRGPAVGAEPLLTILHAGYLWVIIGAALLGASMLADHVPQAAAIHALTAGAIGTMTLGVM
TRVSLGHTGRALTADRITGLIYLLVILAAIARVAAWDGGSSAMLIEISAVLWIGSFGLFVLRYGPILLSPRVKSVDIQPR
R

Specific function: Unknown

COG id: COG3213

COG function: function code P; Uncharacterized protein involved in response to NO

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 6.3.5.4

Molecular weight: Translated: 43111; Mature: 42980

Theoretical pI: Translated: 11.35; Mature: 11.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAMSDVENAKAEPLAIWTIGFRPFFLMAGIWSAVALAGWIAVLTIGLTLPSRFDPLTWHI
CCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
HEMLFGFVLAAIAGFLLTAIPNWTGRPPISGRLLAALVMLWFAGRVTCLVSAWFPLGLAA
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIDLAFPFVLCGVVAREIIAGRNWRNMIMPIPVAMLGVADLLMYLELSGSSLSVGLGWRL
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHH
GLAAIIVLISVVGGRIIPSFTRNWLIRQNASRLPPAHGHVDSLALVSLLAGMIGWALFPE
HHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
RWPFGWLLLVAAALNLWRLGRWRGPAVGAEPLLTILHAGYLWVIIGAALLGASMLADHVP
CCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
QAAAIHALTAGAIGTMTLGVMTRVSLGHTGRALTADRITGLIYLLVILAAIARVAAWDGG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SSAMLIEISAVLWIGSFGLFVLRYGPILLSPRVKSVDIQPRR
CCEEEEEHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCC
>Mature Secondary Structure 
AMSDVENAKAEPLAIWTIGFRPFFLMAGIWSAVALAGWIAVLTIGLTLPSRFDPLTWHI
CCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
HEMLFGFVLAAIAGFLLTAIPNWTGRPPISGRLLAALVMLWFAGRVTCLVSAWFPLGLAA
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIDLAFPFVLCGVVAREIIAGRNWRNMIMPIPVAMLGVADLLMYLELSGSSLSVGLGWRL
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHH
GLAAIIVLISVVGGRIIPSFTRNWLIRQNASRLPPAHGHVDSLALVSLLAGMIGWALFPE
HHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
RWPFGWLLLVAAALNLWRLGRWRGPAVGAEPLLTILHAGYLWVIIGAALLGASMLADHVP
CCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
QAAAIHALTAGAIGTMTLGVMTRVSLGHTGRALTADRITGLIYLLVILAAIARVAAWDGG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SSAMLIEISAVLWIGSFGLFVLRYGPILLSPRVKSVDIQPRR
CCEEEEEHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-aspartate; L-glutamine

Specific reaction: ATP + L-aspartate + L-glutamine = AMP + diphosphate + L-asparagine + L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA