BacMap

Definition	Nitrobacter winogradskyi Nb-255, complete genome.
Accession	NC_007406
Length	3,402,093

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The map label for this gene is sdh [H]

Identifier: 75674675

GI number: 75674675

Start: 534468

End: 535238

Strand: Direct

Name: sdh [H]

Synonym: Nwi_0477

Alternate gene names: 75674675

Gene position: 534468-535238 (Clockwise)

Preceding gene: 75674674

Following gene: 75674676

Centisome position: 15.71

GC content: 63.42

Gene sequence:

>771_bases
GTGACACTGATAACAGGTGCGTCCTCCGGGATTGGCGCCGAGTTCGCGCGGGTATTCGCCGCGCATGGGCACAGGCTGGC
GTTGGTGGCCCGGCGAGCTGATCGTCTCAACGCTCTCGCGGCTGAAATCGTCGCATCGGGACGCATGGCTCCGATCGTCA
TTCCCTGCGACCTCGGCGAGAAGGGCGCGGCGGAGAGGATCGCGGCGGCGCTCACCGCTGAAGGTGTCGAGGTCAAGTAT
CTCGTGAACAACGCCGGCTACGGATTGTCCGGTCATGCCGTCGAACTGGATCACGCTGATCAGCTTGCGCTGATCGACCT
CAACGTCCGGGCGCTGACGGATCTGTCGCTGCGATTTTCCGAAAGCCTGATCCGGCTGCGCGGAGGCATTCTCAACGTCG
GGTCGATCGCCGGATTTCTGCCGGGGCCCGGCATGGCCGTCTATTATGCGTCGAAGGCCTATGTCCTGTCATTCAGCGAG
GCGCTGCGGGGCGAACTCGGTCCTCGCGGCGTCCATGTCACCGTGGTCTGCCCTGGTCCGGTCCCCACTGAATTTCAGGC
GCGGGCGGGTGTGAAATCCGGTTTCCACGCTTCAATCCTGCAGGTATCCGCAAGGGATGTTGCTCTCGAAGGCTATCGAG
GGCTGATGGCAAACAAGAGGGCGGTGCTGCCGGGCCTGGGCGTCAAAATGCTGCCATTGCTGCTTCGGCTGGTTCCGCGC
GATGCAATCCTGCGGGCTGTCGCGGGGTTTCAGGCGCGAAGACGGTTGTGA

Upstream 100 bases:

>100_bases
GGACATGCTTTGCTTGAAAACGCTATAACGCCGGTCGTCGCACCGAACCTTGATCGTGTCTCCTCCAACCGTCTCAAGAG
CGTAGATCGTGATCCATCCG

Downstream 100 bases:

>100_bases
GGCCTCTTCGGACCGCGCGTCCTGGGTACCGATATCGTGGCTGCACCGACGAAAATGTCGTGGTTGCATCGACGAAAATC
GAGGCAGGTTGGTGGATGCG

Product: short-chain dehydrogenase/reductase SDR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MTLITGASSGIGAEFARVFAAHGHRLALVARRADRLNALAAEIVASGRMAPIVIPCDLGEKGAAERIAAALTAEGVEVKY
LVNNAGYGLSGHAVELDHADQLALIDLNVRALTDLSLRFSESLIRLRGGILNVGSIAGFLPGPGMAVYYASKAYVLSFSE
ALRGELGPRGVHVTVVCPGPVPTEFQARAGVKSGFHASILQVSARDVALEGYRGLMANKRAVLPGLGVKMLPLLLRLVPR
DAILRAVAGFQARRRL

Sequences:

>Translated_256_residues
MTLITGASSGIGAEFARVFAAHGHRLALVARRADRLNALAAEIVASGRMAPIVIPCDLGEKGAAERIAAALTAEGVEVKY
LVNNAGYGLSGHAVELDHADQLALIDLNVRALTDLSLRFSESLIRLRGGILNVGSIAGFLPGPGMAVYYASKAYVLSFSE
ALRGELGPRGVHVTVVCPGPVPTEFQARAGVKSGFHASILQVSARDVALEGYRGLMANKRAVLPGLGVKMLPLLLRLVPR
DAILRAVAGFQARRRL
>Mature_255_residues
TLITGASSGIGAEFARVFAAHGHRLALVARRADRLNALAAEIVASGRMAPIVIPCDLGEKGAAERIAAALTAEGVEVKYL
VNNAGYGLSGHAVELDHADQLALIDLNVRALTDLSLRFSESLIRLRGGILNVGSIAGFLPGPGMAVYYASKAYVLSFSEA
LRGELGPRGVHVTVVCPGPVPTEFQARAGVKSGFHASILQVSARDVALEGYRGLMANKRAVLPGLGVKMLPLLLRLVPRD
AILRAVAGFQARRRL

Specific function: Catalyzes the oxidation of the hydroxyl group of serine to form 2-aminomalonate semialdehyde which is spontaneously converted into 2-aminoacetaldehyde and CO(2). Also acts on D- serine, L-glycerate, D-glycerate and 2-methyl-DL-serine. Does not act on O-me

COG id: COG0300

COG function: function code R; Short-chain dehydrogenases of various substrate specificities

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI7705855, Length=188, Percent_Identity=29.2553191489362, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI4557649, Length=189, Percent_Identity=35.4497354497354, Blast_Score=85, Evalue=7e-17,
Organism=Homo sapiens, GI20149619, Length=208, Percent_Identity=30.2884615384615, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI7706318, Length=190, Percent_Identity=33.1578947368421, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI40254992, Length=183, Percent_Identity=30.6010928961749, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI109715829, Length=194, Percent_Identity=29.8969072164948, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI226371731, Length=189, Percent_Identity=27.5132275132275, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI32483357, Length=186, Percent_Identity=26.8817204301075, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI33667109, Length=184, Percent_Identity=27.1739130434783, Blast_Score=65, Evalue=5e-11,
Organism=Homo sapiens, GI5031737, Length=186, Percent_Identity=26.8817204301075, Blast_Score=65, Evalue=5e-11,
Organism=Escherichia coli, GI1787820, Length=190, Percent_Identity=35.2631578947368, Blast_Score=91, Evalue=5e-20,
Organism=Escherichia coli, GI1787335, Length=188, Percent_Identity=30.3191489361702, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI87082100, Length=183, Percent_Identity=31.1475409836066, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI87082160, Length=183, Percent_Identity=27.8688524590164, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1786701, Length=185, Percent_Identity=29.1891891891892, Blast_Score=68, Evalue=5e-13,
Organism=Escherichia coli, GI2367365, Length=184, Percent_Identity=32.6086956521739, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71994604, Length=209, Percent_Identity=31.5789473684211, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71994600, Length=210, Percent_Identity=31.4285714285714, Blast_Score=95, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17562908, Length=207, Percent_Identity=31.8840579710145, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17562990, Length=188, Percent_Identity=31.9148936170213, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17560150, Length=194, Percent_Identity=30.9278350515464, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17562906, Length=230, Percent_Identity=30, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI193204405, Length=207, Percent_Identity=32.8502415458937, Blast_Score=76, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17563726, Length=209, Percent_Identity=27.2727272727273, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17551412, Length=234, Percent_Identity=28.6324786324786, Blast_Score=74, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17531453, Length=189, Percent_Identity=31.2169312169312, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17560332, Length=199, Percent_Identity=29.6482412060301, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17562904, Length=234, Percent_Identity=26.9230769230769, Blast_Score=71, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI72000259, Length=206, Percent_Identity=28.1553398058252, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17559104, Length=198, Percent_Identity=28.2828282828283, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17560220, Length=190, Percent_Identity=31.0526315789474, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17562910, Length=199, Percent_Identity=27.6381909547739, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17538480, Length=231, Percent_Identity=25.974025974026, Blast_Score=67, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6319635, Length=188, Percent_Identity=27.6595744680851, Blast_Score=77, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6323882, Length=191, Percent_Identity=34.5549738219895, Blast_Score=76, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6322067, Length=186, Percent_Identity=27.9569892473118, Blast_Score=62, Evalue=8e-11,
Organism=Drosophila melanogaster, GI28571526, Length=201, Percent_Identity=35.3233830845771, Blast_Score=114, Evalue=5e-26,
Organism=Drosophila melanogaster, GI21357041, Length=200, Percent_Identity=35.5, Blast_Score=110, Evalue=9e-25,
Organism=Drosophila melanogaster, GI24644339, Length=209, Percent_Identity=33.4928229665072, Blast_Score=104, Evalue=6e-23,
Organism=Drosophila melanogaster, GI28574402, Length=190, Percent_Identity=33.6842105263158, Blast_Score=97, Evalue=9e-21,
Organism=Drosophila melanogaster, GI24584744, Length=190, Percent_Identity=33.6842105263158, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24584752, Length=188, Percent_Identity=34.5744680851064, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24584748, Length=263, Percent_Identity=30.7984790874525, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI161076933, Length=194, Percent_Identity=32.9896907216495, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI23397609, Length=201, Percent_Identity=33.8308457711443, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24640442, Length=189, Percent_Identity=33.3333333333333, Blast_Score=88, Evalue=5e-18,
Organism=Drosophila melanogaster, GI21358495, Length=203, Percent_Identity=31.0344827586207, Blast_Score=81, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24580925, Length=197, Percent_Identity=30.9644670050761, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI116007236, Length=194, Percent_Identity=29.3814432989691, Blast_Score=71, Evalue=6e-13,
Organism=Drosophila melanogaster, GI28571387, Length=194, Percent_Identity=30.4123711340206, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24641232, Length=195, Percent_Identity=29.2307692307692, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24644337, Length=209, Percent_Identity=34.9282296650718, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24643142, Length=192, Percent_Identity=33.3333333333333, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24648944, Length=220, Percent_Identity=30.9090909090909, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24641388, Length=194, Percent_Identity=32.9896907216495, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: 1300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.1.1.276 [H]

Molecular weight: Translated: 26921; Mature: 26790

Theoretical pI: Translated: 10.42; Mature: 10.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLITGASSGIGAEFARVFAAHGHRLALVARRADRLNALAAEIVASGRMAPIVIPCDLGE
CEEEECCCCCCHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHCCCCCEEEEECCCCC
KGAAERIAAALTAEGVEVKYLVNNAGYGLSGHAVELDHADQLALIDLNVRALTDLSLRFS
CCHHHHHHHHHHHCCEEEEEEEECCCCCCCCCEEEECCCCCEEEEEECHHHHHHHHHHHH
ESLIRLRGGILNVGSIAGFLPGPGMAVYYASKAYVLSFSEALRGELGPRGVHVTVVCPGP
HHHHHHHCCEEEHHHHHCCCCCCCEEEEEECCEEEEEEHHHHCCCCCCCCEEEEEEECCC
VPTEFQARAGVKSGFHASILQVSARDVALEGYRGLMANKRAVLPGLGVKMLPLLLRLVPR
CCCCHHHHCCCCCCCCHHHEEEHHHHEEHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCH
DAILRAVAGFQARRRL
HHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TLITGASSGIGAEFARVFAAHGHRLALVARRADRLNALAAEIVASGRMAPIVIPCDLGE
EEEECCCCCCHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHCCCCCEEEEECCCCC
KGAAERIAAALTAEGVEVKYLVNNAGYGLSGHAVELDHADQLALIDLNVRALTDLSLRFS
CCHHHHHHHHHHHCCEEEEEEEECCCCCCCCCEEEECCCCCEEEEEECHHHHHHHHHHHH
ESLIRLRGGILNVGSIAGFLPGPGMAVYYASKAYVLSFSEALRGELGPRGVHVTVVCPGP
HHHHHHHCCEEEHHHHHCCCCCCCEEEEEECCEEEEEEHHHHCCCCCCCCEEEEEEECCC
VPTEFQARAGVKSGFHASILQVSARDVALEGYRGLMANKRAVLPGLGVKMLPLLLRLVPR
CCCCHHHHCCCCCCCCHHHEEEHHHHEEHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCH
DAILRAVAGFQARRRL
HHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]