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The map label for this gene is rlmH

Identifier: 75674645

GI number: 75674645

Start: 503329

End: 503811

Strand: Direct

Name: rlmH

Synonym: Nwi_0447

Alternate gene names: 75674645

Gene position: 503329-503811 (Clockwise)

Preceding gene: 75674644

Following gene: 75674646

Centisome position: 14.79

GC content: 62.94

Gene sequence:

>483_bases
ATGCGACTCGTCGTCATTGCCGTGGGGCGTCTGAAGCAGGGGCCTGAGCGGGAGCTTGCCGATCGCTATCGCGGACGCTT
CGCGGATATCGGGCGCAATCTTGGCTTTCGCGGCCTGGATGTCCATGAAGTCGCGGAAAGCCGCGCCCGCGATGCCGGGC
AGCGCATTCGGGAAGAGGCGGCGGCCGTCCTTGCGCTGGCACCCAAGGAGATGATCCTGGTGGCGCTGGACGAAAAAGGC
AAAAGTATCGACAGCGCCGCATTTGCGGAACATCTGGGCCGCTGGAGGGATGAGTCGGTGGCGGACGCGGTTTTCATGAT
CGGTGGCGCGGACGGACTTTCGCCTGAATTACGGCGCAGGGCTAGATTGAGCGTTGCATTCGGCGCGGCCACATGGCCGC
ATCAGATGGTGCGCGTCATGCTGCTTGAGCAGATCTATCGGGCCGCGACCATTCTGGCCGGTCATCCTTATCACCGTGGT
TAG

Upstream 100 bases:

>100_bases
CTACAATCTCGAGAGGTTGTGGATACAGAACACGGCGACGGCGAAGACGGTCTGAGCCGTCCGCCGCCGCGTTTCAATTA
TTGCATGTCAGCGTGCGTGG

Downstream 100 bases:

>100_bases
CGTGCGATGCTGAACGCGCTGGCGCGACCGGATTGATATCGTGCGAACATACGAGCGCCGATCATCTGAACTGGAGTTGA
TGCGGGGTCTGCGCGTGCGG

Product: rRNA large subunit methyltransferase

Products: NA

Alternate protein names: 23S rRNA m3Psi1915 methyltransferase; rRNA (pseudouridine-N3-)-methyltransferase rlmH

Number of amino acids: Translated: 160; Mature: 160

Protein sequence:

>160_residues
MRLVVIAVGRLKQGPERELADRYRGRFADIGRNLGFRGLDVHEVAESRARDAGQRIREEAAAVLALAPKEMILVALDEKG
KSIDSAAFAEHLGRWRDESVADAVFMIGGADGLSPELRRRARLSVAFGAATWPHQMVRVMLLEQIYRAATILAGHPYHRG

Sequences:

>Translated_160_residues
MRLVVIAVGRLKQGPERELADRYRGRFADIGRNLGFRGLDVHEVAESRARDAGQRIREEAAAVLALAPKEMILVALDEKG
KSIDSAAFAEHLGRWRDESVADAVFMIGGADGLSPELRRRARLSVAFGAATWPHQMVRVMLLEQIYRAATILAGHPYHRG
>Mature_160_residues
MRLVVIAVGRLKQGPERELADRYRGRFADIGRNLGFRGLDVHEVAESRARDAGQRIREEAAAVLALAPKEMILVALDEKG
KSIDSAAFAEHLGRWRDESVADAVFMIGGADGLSPELRRRARLSVAFGAATWPHQMVRVMLLEQIYRAATILAGHPYHRG

Specific function: Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA

COG id: COG1576

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase rlmH family

Homologues:

Organism=Escherichia coli, GI1786855, Length=159, Percent_Identity=33.3333333333333, Blast_Score=80, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMH_NITWN (Q3SVH7)

Other databases:

- EMBL:   CP000115
- RefSeq:   YP_317066.1
- ProteinModelPortal:   Q3SVH7
- SMR:   Q3SVH7
- STRING:   Q3SVH7
- GeneID:   3676622
- GenomeReviews:   CP000115_GR
- KEGG:   nwi:Nwi_0447
- NMPDR:   fig|323098.3.peg.139
- eggNOG:   COG1576
- HOGENOM:   HBG622807
- OMA:   ILSGHPY
- PhylomeDB:   Q3SVH7
- ProtClustDB:   PRK00103
- BioCyc:   NWIN323098:NWI_0447-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00658
- InterPro:   IPR003742
- PIRSF:   PIRSF004505

Pfam domain/function: PF02590 SPOUT_MTase

EC number: NA

Molecular weight: Translated: 17711; Mature: 17711

Theoretical pI: Translated: 10.32; Mature: 10.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLVVIAVGRLKQGPERELADRYRGRFADIGRNLGFRGLDVHEVAESRARDAGQRIREEA
CEEEEEEECCCCCCCHHHHHHHHCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
AAVLALAPKEMILVALDEKGKSIDSAAFAEHLGRWRDESVADAVFMIGGADGLSPELRRR
HHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCHHHHHHHEEECCCCCCHHHHHH
ARLSVAFGAATWPHQMVRVMLLEQIYRAATILAGHPYHRG
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MRLVVIAVGRLKQGPERELADRYRGRFADIGRNLGFRGLDVHEVAESRARDAGQRIREEA
CEEEEEEECCCCCCCHHHHHHHHCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
AAVLALAPKEMILVALDEKGKSIDSAAFAEHLGRWRDESVADAVFMIGGADGLSPELRRR
HHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCHHHHHHHEEECCCCCCHHHHHH
ARLSVAFGAATWPHQMVRVMLLEQIYRAATILAGHPYHRG
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA