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The map label for this gene is gltD [H]

Identifier: 74318786

GI number: 74318786

Start: 2849783

End: 2851228

Strand: Direct

Name: gltD [H]

Synonym: Tbd_2768

Alternate gene names: 74318786

Gene position: 2849783-2851228 (Clockwise)

Preceding gene: 74318785

Following gene: 74318787

Centisome position: 97.94

GC content: 67.91

Gene sequence:

>1446_bases
ATGGGTGACGTATTCCAGTTCCTGAAGCAGTCGCGCCGCGACGGCGCGAAGACGCCTGCCGACGTGCGCAAGCATGAATT
CCGCGAGATCTACGCGCTGATGGACCCGGCGCACGCGCAGGAGCAGGCCGACCGCTGCCTCGGCTGCGGCAACCCCTACT
GCGAGTGGAAGTGCCCGGTGCACAACTACATCCCGAACTGGCTCAAGCTCGTGAACGAAGGCCGCCTGTTCGAGGCGGCC
GAACTCTCGCACCAGACCAATTCGCTGCCCGAGGTGTGCGGCCGCGTGTGTCCGCAGGACCGGCTCTGCGAAGGCGCCTG
CACGCTCAACACCGACGGCTTCGGTGAAGCGGTCGGCGGCGGCCCGAACACGGGCGGCTTCGGCGCGGTCACGATCGGCC
AGATCGAGCGCTACATTTCCGAGGAGGCATTCAAGGCCGGCTGGCGGCCCGACCTCTCGAACGTCGTGCCGAGCGGCAGG
AGGGTCGCCGTGATCGGCGCAGGCCCGGCCGGCCTCGCCTGCGCCGACGTGCTTGCGCGCAACGGCGTGCAAGCGGTGGT
CTACGACCGCTACGACGCGATCGGCGGCCTGCTGACCTTCGGCATCCCCGAGTTCAAGATGGAAAAGACCGTCATGACCC
GCCGCCGCGAGGTATTCGAGGGCATGGGCATCGAATTCCGTCTCAACACCGAGGTCGGCCGCGACGTCTCGATGCAGACC
TTGCTCGACGAATACGACGCCGTATTCATGGGCATGGGCACCTACACCTACATGAAGGGCGGCTTCCCGGGCGAGGACCT
GCCGGGCGTGCTCGAAGCGCTGCCCTTCCTGATTTCCAACGTCCGCCAATGCCTCGAACTCGCCGGTCCGGACGAGGCCT
TCCACGACATGAAGGGCAAGCGCGTCGTCGTCCTCGGCGGCGGCGACACGGCGATGGACTGCAACCGCACGAGCATCCGC
CAGGGCGCGGCGTCCGTCGTCTGCGCCTACCGGCGCGACGAGAAGAACATGCCCGGCTCCAAGCGCGAAGTCGCCAACGC
CAAGGAAGAAGGCGTGCAATTCCTGTGGAACCGCCAGCCGGTCGAGATCGTAGGCAACGGCCGCGTCGAGGGCGTCAAGC
TCGTCACGACCGAACTCGGCCCGCCCGACGCGCGCGGCCGCCGCACGCCGGTCGTCATTCCGGGTTCTGAGGAAATCATC
GCAGCCGACCACGTCATAGTCGCCTTCGGCTTCCGCCCGAACCCGCAGCCCTGGTTCGCCGAGCACGCGATCGCGACCGA
CCCCGCCGGGCGTGTCATCGCGAGCGGCCAGGCGCACCCCTACCAGACCGCCAACCCCAAGGTCTTCGCCGGCGGCGACA
TGGTCCGCGGTTCCGACCTCGTCGTGACCGCGGTGTGGGAAGGCCGCGAGGCCGCCAAGGGTATCCTCGCCTACCTCGAG
ATCTAG

Upstream 100 bases:

>100_bases
CGGCCCTGGCGAAGGCCTGGCTGGTCAAGCCGAAGCGCATTTCGATCGAAGACCTGGTCGAGGATCTGCCCGGCCAGCAA
CGTGAAGTATTGGAAGCATA

Downstream 100 bases:

>100_bases
CATCGCCCGCGCGCCGGCGGCTGCCGGCGCGCGAACCGGGTATGCTGCGACAATGTCCGCGCCTTCAGCCCGCATGCTTT
CCGGTCCCTATTACCCGCGC

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: Glutamate synthase subunit beta; GLTS beta chain; NADPH-GOGAT [H]

Number of amino acids: Translated: 481; Mature: 480

Protein sequence:

>481_residues
MGDVFQFLKQSRRDGAKTPADVRKHEFREIYALMDPAHAQEQADRCLGCGNPYCEWKCPVHNYIPNWLKLVNEGRLFEAA
ELSHQTNSLPEVCGRVCPQDRLCEGACTLNTDGFGEAVGGGPNTGGFGAVTIGQIERYISEEAFKAGWRPDLSNVVPSGR
RVAVIGAGPAGLACADVLARNGVQAVVYDRYDAIGGLLTFGIPEFKMEKTVMTRRREVFEGMGIEFRLNTEVGRDVSMQT
LLDEYDAVFMGMGTYTYMKGGFPGEDLPGVLEALPFLISNVRQCLELAGPDEAFHDMKGKRVVVLGGGDTAMDCNRTSIR
QGAASVVCAYRRDEKNMPGSKREVANAKEEGVQFLWNRQPVEIVGNGRVEGVKLVTTELGPPDARGRRTPVVIPGSEEII
AADHVIVAFGFRPNPQPWFAEHAIATDPAGRVIASGQAHPYQTANPKVFAGGDMVRGSDLVVTAVWEGREAAKGILAYLE
I

Sequences:

>Translated_481_residues
MGDVFQFLKQSRRDGAKTPADVRKHEFREIYALMDPAHAQEQADRCLGCGNPYCEWKCPVHNYIPNWLKLVNEGRLFEAA
ELSHQTNSLPEVCGRVCPQDRLCEGACTLNTDGFGEAVGGGPNTGGFGAVTIGQIERYISEEAFKAGWRPDLSNVVPSGR
RVAVIGAGPAGLACADVLARNGVQAVVYDRYDAIGGLLTFGIPEFKMEKTVMTRRREVFEGMGIEFRLNTEVGRDVSMQT
LLDEYDAVFMGMGTYTYMKGGFPGEDLPGVLEALPFLISNVRQCLELAGPDEAFHDMKGKRVVVLGGGDTAMDCNRTSIR
QGAASVVCAYRRDEKNMPGSKREVANAKEEGVQFLWNRQPVEIVGNGRVEGVKLVTTELGPPDARGRRTPVVIPGSEEII
AADHVIVAFGFRPNPQPWFAEHAIATDPAGRVIASGQAHPYQTANPKVFAGGDMVRGSDLVVTAVWEGREAAKGILAYLE
I
>Mature_480_residues
GDVFQFLKQSRRDGAKTPADVRKHEFREIYALMDPAHAQEQADRCLGCGNPYCEWKCPVHNYIPNWLKLVNEGRLFEAAE
LSHQTNSLPEVCGRVCPQDRLCEGACTLNTDGFGEAVGGGPNTGGFGAVTIGQIERYISEEAFKAGWRPDLSNVVPSGRR
VAVIGAGPAGLACADVLARNGVQAVVYDRYDAIGGLLTFGIPEFKMEKTVMTRRREVFEGMGIEFRLNTEVGRDVSMQTL
LDEYDAVFMGMGTYTYMKGGFPGEDLPGVLEALPFLISNVRQCLELAGPDEAFHDMKGKRVVVLGGGDTAMDCNRTSIRQ
GAASVVCAYRRDEKNMPGSKREVANAKEEGVQFLWNRQPVEIVGNGRVEGVKLVTTELGPPDARGRRTPVVIPGSEEIIA
ADHVIVAFGFRPNPQPWFAEHAIATDPAGRVIASGQAHPYQTANPKVFAGGDMVRGSDLVVTAVWEGREAAKGILAYLEI

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=473, Percent_Identity=28.1183932346723, Blast_Score=145, Evalue=1e-34,
Organism=Homo sapiens, GI111118981, Length=158, Percent_Identity=31.6455696202532, Blast_Score=66, Evalue=9e-11,
Organism=Escherichia coli, GI1789606, Length=483, Percent_Identity=64.5962732919255, Blast_Score=645, Evalue=0.0,
Organism=Escherichia coli, GI1788811, Length=474, Percent_Identity=57.1729957805907, Blast_Score=545, Evalue=1e-156,
Organism=Escherichia coli, GI87082180, Length=476, Percent_Identity=53.5714285714286, Blast_Score=499, Evalue=1e-142,
Organism=Escherichia coli, GI1788468, Length=446, Percent_Identity=29.372197309417, Blast_Score=171, Evalue=7e-44,
Organism=Escherichia coli, GI1789243, Length=433, Percent_Identity=29.3302540415704, Blast_Score=123, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17570289, Length=507, Percent_Identity=33.3333333333333, Blast_Score=220, Evalue=1e-57,
Organism=Caenorhabditis elegans, GI71984108, Length=488, Percent_Identity=29.0983606557377, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17543860, Length=162, Percent_Identity=29.0123456790123, Blast_Score=72, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6320030, Length=493, Percent_Identity=33.0628803245436, Blast_Score=226, Evalue=6e-60,
Organism=Drosophila melanogaster, GI24665547, Length=499, Percent_Identity=36.87374749499, Blast_Score=258, Evalue=4e-69,
Organism=Drosophila melanogaster, GI24665543, Length=499, Percent_Identity=36.87374749499, Blast_Score=258, Evalue=4e-69,
Organism=Drosophila melanogaster, GI28574881, Length=499, Percent_Identity=36.87374749499, Blast_Score=258, Evalue=5e-69,
Organism=Drosophila melanogaster, GI24665539, Length=499, Percent_Identity=36.87374749499, Blast_Score=258, Evalue=5e-69,
Organism=Drosophila melanogaster, GI24640763, Length=480, Percent_Identity=26.6666666666667, Blast_Score=142, Evalue=4e-34,
Organism=Drosophila melanogaster, GI18858217, Length=480, Percent_Identity=26.6666666666667, Blast_Score=142, Evalue=4e-34,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006
- InterPro:   IPR009051
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 52311; Mature: 52180

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGDVFQFLKQSRRDGAKTPADVRKHEFREIYALMDPAHAQEQADRCLGCGNPYCEWKCPV
CCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCEEECCH
HNYIPNWLKLVNEGRLFEAAELSHQTNSLPEVCGRVCPQDRLCEGACTLNTDGFGEAVGG
HHHCHHHHHHHCCCCEEHHHHHHHHCCCHHHHHHHHCCHHHHCCCCEEECCCCCCCCCCC
GPNTGGFGAVTIGQIERYISEEAFKAGWRPDLSNVVPSGRRVAVIGAGPAGLACADVLAR
CCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCHHHHCCCCCEEEEEECCCCHHHHHHHHHH
NGVQAVVYDRYDAIGGLLTFGIPEFKMEKTVMTRRREVFEGMGIEFRLNTEVGRDVSMQT
CCCEEEEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHH
LLDEYDAVFMGMGTYTYMKGGFPGEDLPGVLEALPFLISNVRQCLELAGPDEAFHDMKGK
HHHHHHHHEECCCHHEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCC
RVVVLGGGDTAMDCNRTSIRQGAASVVCAYRRDEKNMPGSKREVANAKEEGVQFLWNRQP
EEEEECCCCCCCCCCHHHHHHCHHHEEEEHHCCCCCCCCCHHHHHCCHHHHHHHHCCCCC
VEIVGNGRVEGVKLVTTELGPPDARGRRTPVVIPGSEEIIAADHVIVAFGFRPNPQPWFA
EEEEECCCCCCEEEEEECCCCCCCCCCCCCEEECCCCCEEEECEEEEEECCCCCCCCCHH
EHAIATDPAGRVIASGQAHPYQTANPKVFAGGDMVRGSDLVVTAVWEGREAAKGILAYLE
CCCCCCCCCCCEEECCCCCCCCCCCCEEEECCCEECCCCEEEEEEECCHHHHHHHHHHCC
I
C
>Mature Secondary Structure 
GDVFQFLKQSRRDGAKTPADVRKHEFREIYALMDPAHAQEQADRCLGCGNPYCEWKCPV
CHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCEEECCH
HNYIPNWLKLVNEGRLFEAAELSHQTNSLPEVCGRVCPQDRLCEGACTLNTDGFGEAVGG
HHHCHHHHHHHCCCCEEHHHHHHHHCCCHHHHHHHHCCHHHHCCCCEEECCCCCCCCCCC
GPNTGGFGAVTIGQIERYISEEAFKAGWRPDLSNVVPSGRRVAVIGAGPAGLACADVLAR
CCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCHHHHCCCCCEEEEEECCCCHHHHHHHHHH
NGVQAVVYDRYDAIGGLLTFGIPEFKMEKTVMTRRREVFEGMGIEFRLNTEVGRDVSMQT
CCCEEEEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHH
LLDEYDAVFMGMGTYTYMKGGFPGEDLPGVLEALPFLISNVRQCLELAGPDEAFHDMKGK
HHHHHHHHEECCCHHEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCC
RVVVLGGGDTAMDCNRTSIRQGAASVVCAYRRDEKNMPGSKREVANAKEEGVQFLWNRQP
EEEEECCCCCCCCCCHHHHHHCHHHEEEEHHCCCCCCCCCHHHHHCCHHHHHHHHCCCCC
VEIVGNGRVEGVKLVTTELGPPDARGRRTPVVIPGSEEIIAADHVIVAFGFRPNPQPWFA
EEEEECCCCCCEEEEEECCCCCCCCCCCCCEEECCCCCEEEECEEEEEECCCCCCCCCHH
EHAIATDPAGRVIASGQAHPYQTANPKVFAGGDMVRGSDLVVTAVWEGREAAKGILAYLE
CCCCCCCCCCCEEECCCCCCCCCCCCEEEECCCEECCCCEEEEEEECCHHHHHHHHHHCC
I
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3326786; 9278503; 9298646; 9600841; 2643092 [H]