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The map label for this gene is rpfG [H]

Identifier: 74318688

GI number: 74318688

Start: 2743877

End: 2744911

Strand: Direct

Name: rpfG [H]

Synonym: Tbd_2670

Alternate gene names: 74318688

Gene position: 2743877-2744911 (Clockwise)

Preceding gene: 74318686

Following gene: 74318689

Centisome position: 94.3

GC content: 67.63

Gene sequence:

>1035_bases
ATGATCGTCGACGACCGCGGGACCGCGCGCAGCCTGCTCGAAGGCCTGGCACGCACGCTCGAGCCCGGCGTCATCGTTCA
GAGCTACGCGGACCCCTTCGAGGCGCTCGCGCAGATGCAGCTCGCGACGCCCGACCTCATCATCACCGACTACCGCATGC
CCGGCATGGACGGCATCGAGTTCACGCGCAGGATCCGCGCCGAGCGCCGCCTGACCGACGTGCCGGTCGTCATCGTCACG
GTCGTCGAAGACCGCCAGATCCGCTACCAGGCGCTCGAAAACGGCGCGACCGACTTTCTCACCCGGCCGATCGACCCGCA
GGAATGCCGCGCCCGCTGCCTCAACCTGCTCGCCCTGCGGCGCAGCCAGAAGGCGATCGCCGATCGCGCGATGTGGCTCG
AGGAACAGGTCATGCAGGCGACGCGCGAGGTGCGGACGCGCGAACGCGAGACGCTGATGAAACTCGCGAAGGCCGGCGAA
TACCGCGACCAGGAAACCGGCAACCACATCGTCAGGATGGCGAAATACGCCCGACTCATCGCCGAGGAACTCAAGCTCAG
CGCGATGGAATGCGACGAGATCGAAGCCGCGGCGCCGATGCACGACATCGGCAAGATCGGCATCCCTGACGTGATCCTGC
TCAAGCCCGGCCGGCACACGCCCGACGAGCAGCAGATCATGCGCCGCCACCCGCAGCTCGGTCACGAAATCCTCGCCGGC
AGCCCCTCGCGCTACCTGCAGATGGGCGCGATCATCGCCCTCGGCCACCACGAGAAATTCGACGGCACCGGCTATCCGCA
AGGTCTCGCCGGCGAAGACATCCCGCTCCCGGCCCGCATCGTCGCGGTCGCCGACGTCTTCGACGCACTCACCTCGGATC
GCCCCTACAAGCGCGCCTGGTCGTTCCAGGACGCCTTGCAGTTCATCCGCTCGGAAAGCGGTCGCCACTTCGACCCGGCC
TGCGTGCGCGCCTTCGAGCGGCGCATCGATGCAGTCGCGGCGATCGCGCGCGAACTCAGCGATCCGCAGTCCTAA

Upstream 100 bases:

>100_bases
CGAGGTGGCAAACGTGATCGCCAAACCCCTGCGTATCGTGCCTTCTGCGGCCCCCGCCTCGCCGGAGCGGCACGGTCGCC
CGCGCGGCGGGGCGCGTGTG

Downstream 100 bases:

>100_bases
GCGGCCCATGAAGACCGCGCTTCATCAGCTATTGCCTGCATTTCTGCGGCGTCTTCTCAAACGAATAAAAGACCGGCCGG
ATTCAGAGTTCCAGCAGACG

Product: metal dependent phosphohydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MIVDDRGTARSLLEGLARTLEPGVIVQSYADPFEALAQMQLATPDLIITDYRMPGMDGIEFTRRIRAERRLTDVPVVIVT
VVEDRQIRYQALENGATDFLTRPIDPQECRARCLNLLALRRSQKAIADRAMWLEEQVMQATREVRTRERETLMKLAKAGE
YRDQETGNHIVRMAKYARLIAEELKLSAMECDEIEAAAPMHDIGKIGIPDVILLKPGRHTPDEQQIMRRHPQLGHEILAG
SPSRYLQMGAIIALGHHEKFDGTGYPQGLAGEDIPLPARIVAVADVFDALTSDRPYKRAWSFQDALQFIRSESGRHFDPA
CVRAFERRIDAVAAIARELSDPQS

Sequences:

>Translated_344_residues
MIVDDRGTARSLLEGLARTLEPGVIVQSYADPFEALAQMQLATPDLIITDYRMPGMDGIEFTRRIRAERRLTDVPVVIVT
VVEDRQIRYQALENGATDFLTRPIDPQECRARCLNLLALRRSQKAIADRAMWLEEQVMQATREVRTRERETLMKLAKAGE
YRDQETGNHIVRMAKYARLIAEELKLSAMECDEIEAAAPMHDIGKIGIPDVILLKPGRHTPDEQQIMRRHPQLGHEILAG
SPSRYLQMGAIIALGHHEKFDGTGYPQGLAGEDIPLPARIVAVADVFDALTSDRPYKRAWSFQDALQFIRSESGRHFDPA
CVRAFERRIDAVAAIARELSDPQS
>Mature_344_residues
MIVDDRGTARSLLEGLARTLEPGVIVQSYADPFEALAQMQLATPDLIITDYRMPGMDGIEFTRRIRAERRLTDVPVVIVT
VVEDRQIRYQALENGATDFLTRPIDPQECRARCLNLLALRRSQKAIADRAMWLEEQVMQATREVRTRERETLMKLAKAGE
YRDQETGNHIVRMAKYARLIAEELKLSAMECDEIEAAAPMHDIGKIGIPDVILLKPGRHTPDEQQIMRRHPQLGHEILAG
SPSRYLQMGAIIALGHHEKFDGTGYPQGLAGEDIPLPARIVAVADVFDALTSDRPYKRAWSFQDALQFIRSESGRHFDPA
CVRAFERRIDAVAAIARELSDPQS

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=79, Percent_Identity=37.9746835443038, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790299, Length=111, Percent_Identity=34.2342342342342, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1788394, Length=173, Percent_Identity=29.4797687861272, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 38776; Mature: 38776

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVDDRGTARSLLEGLARTLEPGVIVQSYADPFEALAQMQLATPDLIITDYRMPGMDGIE
CCCCCCCHHHHHHHHHHHHCCCCEEEHHHCCHHHHHHHHHHCCCCEEEECCCCCCCCHHH
FTRRIRAERRLTDVPVVIVTVVEDRQIRYQALENGATDFLTRPIDPQECRARCLNLLALR
HHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHHHHH
RSQKAIADRAMWLEEQVMQATREVRTRERETLMKLAKAGEYRDQETGNHIVRMAKYARLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
AEELKLSAMECDEIEAAAPMHDIGKIGIPDVILLKPGRHTPDEQQIMRRHPQLGHEILAG
HHHHHHHHHCHHHHHHHCCHHHHCCCCCCCEEEECCCCCCCCHHHHHHHCHHHHHHHHCC
SPSRYLQMGAIIALGHHEKFDGTGYPQGLAGEDIPLPARIVAVADVFDALTSDRPYKRAW
CHHHHHHHHHEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHC
SFQDALQFIRSESGRHFDPACVRAFERRIDAVAAIARELSDPQS
CHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MIVDDRGTARSLLEGLARTLEPGVIVQSYADPFEALAQMQLATPDLIITDYRMPGMDGIE
CCCCCCCHHHHHHHHHHHHCCCCEEEHHHCCHHHHHHHHHHCCCCEEEECCCCCCCCHHH
FTRRIRAERRLTDVPVVIVTVVEDRQIRYQALENGATDFLTRPIDPQECRARCLNLLALR
HHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHHHHH
RSQKAIADRAMWLEEQVMQATREVRTRERETLMKLAKAGEYRDQETGNHIVRMAKYARLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
AEELKLSAMECDEIEAAAPMHDIGKIGIPDVILLKPGRHTPDEQQIMRRHPQLGHEILAG
HHHHHHHHHCHHHHHHHCCHHHHCCCCCCCEEEECCCCCCCCHHHHHHHCHHHHHHHHCC
SPSRYLQMGAIIALGHHEKFDGTGYPQGLAGEDIPLPARIVAVADVFDALTSDRPYKRAW
CHHHHHHHHHEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHC
SFQDALQFIRSESGRHFDPACVRAFERRIDAVAAIARELSDPQS
CHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]