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The map label for this gene is trmD

Identifier: 74318393

GI number: 74318393

Start: 2450172

End: 2450882

Strand: Direct

Name: trmD

Synonym: Tbd_2375

Alternate gene names: 74318393

Gene position: 2450172-2450882 (Clockwise)

Preceding gene: 74318392

Following gene: 74318394

Centisome position: 84.2

GC content: 67.51

Gene sequence:

>711_bases
ATGCGCTTCGACGCGGTCACGCTCTTCGCCGAGATGTTCGATGCGGTACTGGACTACGGCATCACGCGACGCGCGCTGGA
TCGCGGGCTTTACCGATTCAAGTCGTGGAACCCCCGCGCCTTCACCGACGATCCGCATCGTACGGTCGACGACCGGCCCT
ACGGTGGCGGCCCCGGCATGCTGATGCTGGCCGAGCCCCTGGACCGTGCGCTGAATGCCGTGCAGCAGGAACTGGCGAGC
GAGAATCTGGCGCCGTGGGTGGTGCATTTTTCGCCGCGCGGCCGCCCGCTGACGCAGCAGCGGGTGATGGAACTGAAGGA
CATGCCCGCACTCGTGCTGCTTTGCGGGCGATACGAAGGCATCGACGAGCGGCTGCTGCAGCGCCGCGTGGACGAGGAGG
TCTCGCTCGGCGACTTCGTGCTGTCGGGCGGCGAATTGCCCGCGCTGGCGCTGATGGACGCGATCGTCCGCCAGTTGCCG
GGCGCCCTGAACGACGCGGAGTCGGCGGCACAGGACTCGTTTAGCGCTGGGCTCCTCGACTGCCCGCATTACACGCGACC
CGAGGTCTGGCAGGGCATGGCGGTGCCGCAAGTGCTGAAAAGCGGCAACCACGCGGCAATCGCCAAGTGGCGGCACGAAC
AGAGCCTGCGGCTAACGGGGGCCCTGCGGCCCGACCTGCTGGAACGGGCGCAGCACGATGAACGGAATTGA

Upstream 100 bases:

>100_bases
AAGGCAGGCGCGAACATCTCATTCCGTTCGTCGCGGCCTTCGTCGGCAAGGTCGACCTGGCCGGCGGGACCGTCGAGGTC
GACTGGGGCGAGGACTACTA

Downstream 100 bases:

>100_bases
CGGATAGCCTCCCCGGGCTCTCCGGAATGAGCGGCGCTGGGTTCAACGCGAATCCCGGCCCAATGTGAAACGGCGTCAAG
TCCGCGAGCGGAAAAAAACC

Product: tRNA (Guanine37-N(1)-) methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MRFDAVTLFAEMFDAVLDYGITRRALDRGLYRFKSWNPRAFTDDPHRTVDDRPYGGGPGMLMLAEPLDRALNAVQQELAS
ENLAPWVVHFSPRGRPLTQQRVMELKDMPALVLLCGRYEGIDERLLQRRVDEEVSLGDFVLSGGELPALALMDAIVRQLP
GALNDAESAAQDSFSAGLLDCPHYTRPEVWQGMAVPQVLKSGNHAAIAKWRHEQSLRLTGALRPDLLERAQHDERN

Sequences:

>Translated_236_residues
MRFDAVTLFAEMFDAVLDYGITRRALDRGLYRFKSWNPRAFTDDPHRTVDDRPYGGGPGMLMLAEPLDRALNAVQQELAS
ENLAPWVVHFSPRGRPLTQQRVMELKDMPALVLLCGRYEGIDERLLQRRVDEEVSLGDFVLSGGELPALALMDAIVRQLP
GALNDAESAAQDSFSAGLLDCPHYTRPEVWQGMAVPQVLKSGNHAAIAKWRHEQSLRLTGALRPDLLERAQHDERN
>Mature_236_residues
MRFDAVTLFAEMFDAVLDYGITRRALDRGLYRFKSWNPRAFTDDPHRTVDDRPYGGGPGMLMLAEPLDRALNAVQQELAS
ENLAPWVVHFSPRGRPLTQQRVMELKDMPALVLLCGRYEGIDERLLQRRVDEEVSLGDFVLSGGELPALALMDAIVRQLP
GALNDAESAAQDSFSAGLLDCPHYTRPEVWQGMAVPQVLKSGNHAAIAKWRHEQSLRLTGALRPDLLERAQHDERN

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=228, Percent_Identity=56.140350877193, Blast_Score=255, Evalue=2e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_THIDA (Q3SGC2)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_316133.1
- ProteinModelPortal:   Q3SGC2
- SMR:   Q3SGC2
- STRING:   Q3SGC2
- GeneID:   3671868
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_2375
- NMPDR:   fig|292415.3.peg.2448
- eggNOG:   COG0336
- HOGENOM:   HBG285805
- OMA:   VCGRFEG
- PhylomeDB:   Q3SGC2
- BioCyc:   TDEN292415:TBD_2375-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 26501; Mature: 26501

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: NA

Important sites: BINDING 116-116

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFDAVTLFAEMFDAVLDYGITRRALDRGLYRFKSWNPRAFTDDPHRTVDDRPYGGGPGM
CCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
LMLAEPLDRALNAVQQELASENLAPWVVHFSPRGRPLTQQRVMELKDMPALVLLCGRYEG
EEEHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHCCCC
IDERLLQRRVDEEVSLGDFVLSGGELPALALMDAIVRQLPGALNDAESAAQDSFSAGLLD
HHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCC
CPHYTRPEVWQGMAVPQVLKSGNHAAIAKWRHEQSLRLTGALRPDLLERAQHDERN
CCCCCCHHHHCCCHHHHHHHCCCCHHHHHHHCCCCHHHHCCCCHHHHHHHHCCCCC
>Mature Secondary Structure
MRFDAVTLFAEMFDAVLDYGITRRALDRGLYRFKSWNPRAFTDDPHRTVDDRPYGGGPGM
CCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
LMLAEPLDRALNAVQQELASENLAPWVVHFSPRGRPLTQQRVMELKDMPALVLLCGRYEG
EEEHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHCCCC
IDERLLQRRVDEEVSLGDFVLSGGELPALALMDAIVRQLPGALNDAESAAQDSFSAGLLD
HHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCC
CPHYTRPEVWQGMAVPQVLKSGNHAAIAKWRHEQSLRLTGALRPDLLERAQHDERN
CCCCCCHHHHCCCHHHHHHHCCCCHHHHHHHCCCCHHHHCCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA