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The map label for this gene is glyS

Identifier: 74318365

GI number: 74318365

Start: 2420062

End: 2422167

Strand: Reverse

Name: glyS

Synonym: Tbd_2347

Alternate gene names: 74318365

Gene position: 2422167-2420062 (Counterclockwise)

Preceding gene: 74318366

Following gene: 74318364

Centisome position: 83.24

GC content: 66.71

Gene sequence:

>2106_bases
ATGACCACCGCGAACCTGCTCGTTGAACTCTTCGTCGAGGAATTGCCGCCCAAGGCGCTGAAGAAGCTGGGCGAGGCTTT
TGCCGCCGCGCTGACCGAGAGCCTGGTCGCGCAGGGCCTTGTCCACGCGCAGGCGGCGGTCACCCACTTCGCCTCGCCGC
GCCGCCTCGGTGCGCACATTGAGAATGTGTTGGAACGCTCCGCCGACAAGCCGGTTTCGACCAAACTGATGCCGGTCAGC
GTTGGCCTCGATGCGGATGGCAACGCGACGCCCGCGCTGCTCAAGCGCCTCGCCGCGCTTGGCGCCGACGCTTCTGCGGT
TGCGACGTTGCGCCGTGAATCCGACGGCAAGGCCGAGGCGTTATTCATGGATTCGGTCGCCAAGGGTATCTCCTTGGGGC
TTGGCTTAGCGAAAGCGCTGTGGGAGTCATTGGATAAGCTACCGATCCCGAAAGTCATGAGCTACCAACTTGCTGATGGT
TGGAGCACGGTGAATTTTGTACGTCCCGTTCATGGTTTGGTCGCTCTGCATGGCACTGATCCTGTGGATGTCCGCATGTT
TGGCCTTACCGCGGGCCGCAAGACACACGGTCACCGCTTTGAGGCCGCCGCCGATCCGATCGTGCTCAAGGACGCTGACA
GCTACGCGCAGCAGCTCGAAAGTGAGGGCGCCGTGATTGCTAACTTCGCCGAGCGCCGCGCCGAGATCGTGCGCCAGCTG
GAGGCCGCCGCCGCGCCGTTGGGCCTCGTGCCGGTCGAGGACGAGGCCCTGCTGGACGAGGTCACGGCGCTGGTCGAGCG
CCCCAACGTGCTGCTCGGGCAGTTCGAGCAGGCCTTCCTCGAGGTGCCGCAGGAGTGCCTCATCCTCACCATGAAGGCGA
ACCAGAAGTATTTCCCGCTGCTCGACGCCGCCGGCAAGCTGACGAACAAATTCCTGATCGTGTCCAACATCTCGCCGGCC
GACGCGTCGGCCGTCGTCGGCGGCAACGAGCGCGTCGTGCGCCCGCGGCTGGCCGACGCGAAATTCTTCTTCGACCAGGA
CCGCAAGAAGACGCTCGATGCGCGCGTGCTCGGCCTCGCCAAGGTCGTGTATCACAACAAGCTCGGCACGCAGGGTGAAC
GCGTCACCCGCGTCCAGGCGGTCGCGCGCGCGATCGGCGAGAAGCTCGGCGGCGAGGCGCTGGCGCTCAAGGCCGACCAG
GCCGCATTGCTCGCCAAGGCCGACCTGCTTACCGACATGGTCGGCGAGTTCCCCGAACTGCAGGGCGTCATGGGGCGTTA
TTACGCGCAGCACGACGGCGTCGCCGACGACGTCGCGTTCGCGATCGAGGACCACTACCGGCCACGCTTCGCCGGCGACG
ACCTGCCGCGTAACCCGGTCGGCGTCTGCGTCGCGCTCGCCGACAAGCTCGAGACGCTCGTCGGCCTGTTCGGCATCGGC
GAGAAGCCGACCGGCGACAAGGACCCGTTCGCGCTGCGTCGCCACGCCCTCGGGGTGGTCCGCATGCTGATCGAGAAGCA
CTTGCCGATCGGCCTGAACGAGCTCGTCAGCATCGCCTTTTCCGCTTTCCAGCAGGGGCTACTCGGACAGGCGCACACCG
ATGTCTACCTGTTCATGTTCGAGCGCCTGAGTGGCGCCATGCGCGAGCAGGGCTATTCGGCGAACGAGGTCGATGCGGTG
CTCAGCCTGAATCCGATCCGGATCGATCTCGTGCCGCAGCAACTCGAGGCGGTGCGCGCCTTCGCCGCGCTGCCGGAAGC
CGCAGCGCTCGCCGCGGCCAACAAGCGCGTCGGCAATATCCTGAAAAAAGCCGAGGGCGAGGTCGGCGCCAGCGCCGATC
CGGGCCGCTTTGTCGAGGCTGCGGAGCGCGCACTGTTCGCAGCCCTGGACGAGGTGGCGCCGCGTGCCGCCGCGGCCTTC
GGCAGCGGCGACTACACCGCCTCGCTGCAGGCGCTCGCCGCGCTCAAGGCACCGGTCGACGCCTTCTTCGACCAGGTCAT
GGTCAACGCCGACGACCCGGCGCTCAGGGCCAACCGGCTCGCGCTGCTGAATCAGCTGCACCGGACGATGAACCGCGTGG
CTGACATCTCCCGGCTCGCCGCCTAA

Upstream 100 bases:

>100_bases
ACAGCCGGGCGCGCCTCGGCTTCCCGATGGCGCCCCGTGCCTGGGCCGACGAAGTGCTCGCGAACATCGAAAAACAGACG
CAAAAGAAGGCGGCCGCCGC

Downstream 100 bases:

>100_bases
ACTTCCGCTATGAAACTCATCATTCTCGACCGCGACGGCGTCATCAACTTCGATTCCGACCACTTCATCAAGTCGCCGGA
CGAATGGAAGCCGATTCCCG

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS

Number of amino acids: Translated: 701; Mature: 700

Protein sequence:

>701_residues
MTTANLLVELFVEELPPKALKKLGEAFAAALTESLVAQGLVHAQAAVTHFASPRRLGAHIENVLERSADKPVSTKLMPVS
VGLDADGNATPALLKRLAALGADASAVATLRRESDGKAEALFMDSVAKGISLGLGLAKALWESLDKLPIPKVMSYQLADG
WSTVNFVRPVHGLVALHGTDPVDVRMFGLTAGRKTHGHRFEAAADPIVLKDADSYAQQLESEGAVIANFAERRAEIVRQL
EAAAAPLGLVPVEDEALLDEVTALVERPNVLLGQFEQAFLEVPQECLILTMKANQKYFPLLDAAGKLTNKFLIVSNISPA
DASAVVGGNERVVRPRLADAKFFFDQDRKKTLDARVLGLAKVVYHNKLGTQGERVTRVQAVARAIGEKLGGEALALKADQ
AALLAKADLLTDMVGEFPELQGVMGRYYAQHDGVADDVAFAIEDHYRPRFAGDDLPRNPVGVCVALADKLETLVGLFGIG
EKPTGDKDPFALRRHALGVVRMLIEKHLPIGLNELVSIAFSAFQQGLLGQAHTDVYLFMFERLSGAMREQGYSANEVDAV
LSLNPIRIDLVPQQLEAVRAFAALPEAAALAAANKRVGNILKKAEGEVGASADPGRFVEAAERALFAALDEVAPRAAAAF
GSGDYTASLQALAALKAPVDAFFDQVMVNADDPALRANRLALLNQLHRTMNRVADISRLAA

Sequences:

>Translated_701_residues
MTTANLLVELFVEELPPKALKKLGEAFAAALTESLVAQGLVHAQAAVTHFASPRRLGAHIENVLERSADKPVSTKLMPVS
VGLDADGNATPALLKRLAALGADASAVATLRRESDGKAEALFMDSVAKGISLGLGLAKALWESLDKLPIPKVMSYQLADG
WSTVNFVRPVHGLVALHGTDPVDVRMFGLTAGRKTHGHRFEAAADPIVLKDADSYAQQLESEGAVIANFAERRAEIVRQL
EAAAAPLGLVPVEDEALLDEVTALVERPNVLLGQFEQAFLEVPQECLILTMKANQKYFPLLDAAGKLTNKFLIVSNISPA
DASAVVGGNERVVRPRLADAKFFFDQDRKKTLDARVLGLAKVVYHNKLGTQGERVTRVQAVARAIGEKLGGEALALKADQ
AALLAKADLLTDMVGEFPELQGVMGRYYAQHDGVADDVAFAIEDHYRPRFAGDDLPRNPVGVCVALADKLETLVGLFGIG
EKPTGDKDPFALRRHALGVVRMLIEKHLPIGLNELVSIAFSAFQQGLLGQAHTDVYLFMFERLSGAMREQGYSANEVDAV
LSLNPIRIDLVPQQLEAVRAFAALPEAAALAAANKRVGNILKKAEGEVGASADPGRFVEAAERALFAALDEVAPRAAAAF
GSGDYTASLQALAALKAPVDAFFDQVMVNADDPALRANRLALLNQLHRTMNRVADISRLAA
>Mature_700_residues
TTANLLVELFVEELPPKALKKLGEAFAAALTESLVAQGLVHAQAAVTHFASPRRLGAHIENVLERSADKPVSTKLMPVSV
GLDADGNATPALLKRLAALGADASAVATLRRESDGKAEALFMDSVAKGISLGLGLAKALWESLDKLPIPKVMSYQLADGW
STVNFVRPVHGLVALHGTDPVDVRMFGLTAGRKTHGHRFEAAADPIVLKDADSYAQQLESEGAVIANFAERRAEIVRQLE
AAAAPLGLVPVEDEALLDEVTALVERPNVLLGQFEQAFLEVPQECLILTMKANQKYFPLLDAAGKLTNKFLIVSNISPAD
ASAVVGGNERVVRPRLADAKFFFDQDRKKTLDARVLGLAKVVYHNKLGTQGERVTRVQAVARAIGEKLGGEALALKADQA
ALLAKADLLTDMVGEFPELQGVMGRYYAQHDGVADDVAFAIEDHYRPRFAGDDLPRNPVGVCVALADKLETLVGLFGIGE
KPTGDKDPFALRRHALGVVRMLIEKHLPIGLNELVSIAFSAFQQGLLGQAHTDVYLFMFERLSGAMREQGYSANEVDAVL
SLNPIRIDLVPQQLEAVRAFAALPEAAALAAANKRVGNILKKAEGEVGASADPGRFVEAAERALFAALDEVAPRAAAAFG
SGDYTASLQALAALKAPVDAFFDQVMVNADDPALRANRLALLNQLHRTMNRVADISRLAA

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Escherichia coli, GI1789982, Length=700, Percent_Identity=42.2857142857143, Blast_Score=491, Evalue=1e-140,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYGB_THIDA (Q3SGF0)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_316105.1
- ProteinModelPortal:   Q3SGF0
- STRING:   Q3SGF0
- GeneID:   3673475
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_2347
- NMPDR:   fig|292415.3.peg.2420
- eggNOG:   COG0751
- HOGENOM:   HBG586599
- OMA:   SNITYGH
- PhylomeDB:   Q3SGF0
- ProtClustDB:   PRK01233
- BioCyc:   TDEN292415:TBD_2347-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00255
- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311
- PRINTS:   PR01045
- SMART:   SM00836
- TIGRFAMs:   TIGR00211

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f

EC number: =6.1.1.14

Molecular weight: Translated: 74977; Mature: 74846

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTANLLVELFVEELPPKALKKLGEAFAAALTESLVAQGLVHAQAAVTHFASPRRLGAHI
CCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
ENVLERSADKPVSTKLMPVSVGLDADGNATPALLKRLAALGADASAVATLRRESDGKAEA
HHHHHHCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCHHH
LFMDSVAKGISLGLGLAKALWESLDKLPIPKVMSYQLADGWSTVNFVRPVHGLVALHGTD
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHEEEECCCC
PVDVRMFGLTAGRKTHGHRFEAAADPIVLKDADSYAQQLESEGAVIANFAERRAEIVRQL
CCEEEEEEECCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHCCCEEHHHHHHHHHHHHHH
EAAAAPLGLVPVEDEALLDEVTALVERPNVLLGQFEQAFLEVPQECLILTMKANQKYFPL
HHHHCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHEEEEEECCCCCCCH
LDAAGKLTNKFLIVSNISPADASAVVGGNERVVRPRLADAKFFFDQDRKKTLDARVLGLA
HHHHHHHCCCEEEEECCCCCCCCEEECCCCCEECCCCCCHHHHHCCHHHHHHHHHHHHHH
KVVYHNKLGTQGERVTRVQAVARAIGEKLGGEALALKADQAALLAKADLLTDMVGEFPEL
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHCCHHH
QGVMGRYYAQHDGVADDVAFAIEDHYRPRFAGDDLPRNPVGVCVALADKLETLVGLFGIG
HHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
EKPTGDKDPFALRRHALGVVRMLIEKHLPIGLNELVSIAFSAFQQGLLGQAHTDVYLFMF
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
ERLSGAMREQGYSANEVDAVLSLNPIRIDLVPQQLEAVRAFAALPEAAALAAANKRVGNI
HHHHHHHHHCCCCCCHHHHHEECCCEEEEECHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
LKKAEGEVGASADPGRFVEAAERALFAALDEVAPRAAAAFGSGDYTASLQALAALKAPVD
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
AFFDQVMVNADDPALRANRLALLNQLHRTMNRVADISRLAA
HHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TTANLLVELFVEELPPKALKKLGEAFAAALTESLVAQGLVHAQAAVTHFASPRRLGAHI
CHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
ENVLERSADKPVSTKLMPVSVGLDADGNATPALLKRLAALGADASAVATLRRESDGKAEA
HHHHHHCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCHHH
LFMDSVAKGISLGLGLAKALWESLDKLPIPKVMSYQLADGWSTVNFVRPVHGLVALHGTD
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHEEEECCCC
PVDVRMFGLTAGRKTHGHRFEAAADPIVLKDADSYAQQLESEGAVIANFAERRAEIVRQL
CCEEEEEEECCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHCCCEEHHHHHHHHHHHHHH
EAAAAPLGLVPVEDEALLDEVTALVERPNVLLGQFEQAFLEVPQECLILTMKANQKYFPL
HHHHCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHEEEEEECCCCCCCH
LDAAGKLTNKFLIVSNISPADASAVVGGNERVVRPRLADAKFFFDQDRKKTLDARVLGLA
HHHHHHHCCCEEEEECCCCCCCCEEECCCCCEECCCCCCHHHHHCCHHHHHHHHHHHHHH
KVVYHNKLGTQGERVTRVQAVARAIGEKLGGEALALKADQAALLAKADLLTDMVGEFPEL
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHCCHHH
QGVMGRYYAQHDGVADDVAFAIEDHYRPRFAGDDLPRNPVGVCVALADKLETLVGLFGIG
HHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
EKPTGDKDPFALRRHALGVVRMLIEKHLPIGLNELVSIAFSAFQQGLLGQAHTDVYLFMF
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
ERLSGAMREQGYSANEVDAVLSLNPIRIDLVPQQLEAVRAFAALPEAAALAAANKRVGNI
HHHHHHHHHCCCCCCHHHHHEECCCEEEEECHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
LKKAEGEVGASADPGRFVEAAERALFAALDEVAPRAAAAFGSGDYTASLQALAALKAPVD
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
AFFDQVMVNADDPALRANRLALLNQLHRTMNRVADISRLAA
HHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA