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The map label for this gene is gloA [H]

Identifier: 74318361

GI number: 74318361

Start: 2417595

End: 2418008

Strand: Reverse

Name: gloA [H]

Synonym: Tbd_2343

Alternate gene names: 74318361

Gene position: 2418008-2417595 (Counterclockwise)

Preceding gene: 74318362

Following gene: 74318354

Centisome position: 83.1

GC content: 66.91

Gene sequence:

>414_bases
ATGCGACTACTCCATACCATGCTGCGGGTCGGCGATCTCGACCGCTCGATCGACTTCTACACCCGCGTCCTCGGCATGAC
GCTGCTGCGCCGCAAGGACTACCCGGACGGCAAGTTCACCCTCGCTTTCCTCGGCTACGAGCCCGAAGACCGGGCCGCCG
TGCTCGAACTGACCTACAACTGGGGCGTCGACCGCTACGAGATCGGCACGGGCTACGGCCATATCGCGATCGAGGTCGAC
GACGCCGCGGCAGCCTGCACTGCGGCCGCCGACAAGGGCGTGCGCATCCTGCGCCCGGCAGGCCCGATGGCGAACGGCAG
CACGGTCATCGCCTTCGTCGAGGACCCCGACGGCTATCCGATCGAGTTCATCCAGAAGGGAACGGCCGGCTGGGTCAAGG
AGTGGGCGGAGTAG

Upstream 100 bases:

>100_bases
AAATGTTTTCCTGTCCGCCGACTATCTAGACTGAATACGGCAGACGCACCGAGTCCGACAAAGCGGGGACGGCACGTCGA
AAACCGAACGGAGACCACTC

Downstream 100 bases:

>100_bases
GGGCCGTCCCTAGTCGCTGATCGCCTTGGTGATCAGCGGGATCAGGGGCTCGGGGAGCTTGATCTTGCCGGCCAGCGCGA
AGGCCTGCGCGTCCTTCGAC

Product: lactoylglutathione lyase

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 137; Mature: 137

Protein sequence:

>137_residues
MRLLHTMLRVGDLDRSIDFYTRVLGMTLLRRKDYPDGKFTLAFLGYEPEDRAAVLELTYNWGVDRYEIGTGYGHIAIEVD
DAAAACTAAADKGVRILRPAGPMANGSTVIAFVEDPDGYPIEFIQKGTAGWVKEWAE

Sequences:

>Translated_137_residues
MRLLHTMLRVGDLDRSIDFYTRVLGMTLLRRKDYPDGKFTLAFLGYEPEDRAAVLELTYNWGVDRYEIGTGYGHIAIEVD
DAAAACTAAADKGVRILRPAGPMANGSTVIAFVEDPDGYPIEFIQKGTAGWVKEWAE
>Mature_137_residues
MRLLHTMLRVGDLDRSIDFYTRVLGMTLLRRKDYPDGKFTLAFLGYEPEDRAAVLELTYNWGVDRYEIGTGYGHIAIEVD
DAAAACTAAADKGVRILRPAGPMANGSTVIAFVEDPDGYPIEFIQKGTAGWVKEWAE

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=147, Percent_Identity=40.1360544217687, Blast_Score=101, Evalue=2e-22,
Organism=Escherichia coli, GI1787940, Length=130, Percent_Identity=65.3846153846154, Blast_Score=181, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI17552228, Length=90, Percent_Identity=34.4444444444444, Blast_Score=65, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323639, Length=146, Percent_Identity=43.1506849315069, Blast_Score=96, Evalue=2e-21,
Organism=Drosophila melanogaster, GI20129743, Length=145, Percent_Identity=41.3793103448276, Blast_Score=96, Evalue=1e-20,
Organism=Drosophila melanogaster, GI19920404, Length=139, Percent_Identity=28.7769784172662, Blast_Score=72, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 15217; Mature: 15217

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS00934 GLYOXALASE_I_1 ; PS00935 GLYOXALASE_I_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLLHTMLRVGDLDRSIDFYTRVLGMTLLRRKDYPDGKFTLAFLGYEPEDRAAVLELTYN
CHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCEEEEEEEEC
WGVDRYEIGTGYGHIAIEVDDAAAACTAAADKGVRILRPAGPMANGSTVIAFVEDPDGYP
CCCCEEEECCCCEEEEEEECCCHHHHHHHCCCCEEEEECCCCCCCCCEEEEEEECCCCCC
IEFIQKGTAGWVKEWAE
CCEEECCCCHHHHHHCC
>Mature Secondary Structure
MRLLHTMLRVGDLDRSIDFYTRVLGMTLLRRKDYPDGKFTLAFLGYEPEDRAAVLELTYN
CHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCEEEEEEEEC
WGVDRYEIGTGYGHIAIEVDDAAAACTAAADKGVRILRPAGPMANGSTVIAFVEDPDGYP
CCCCEEEECCCCEEEEEEECCCHHHHHHHCCCCEEEEECCCCCCCCCEEEEEEECCCCCC
IEFIQKGTAGWVKEWAE
CCEEECCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]