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The map label for this gene is ptxB [H]

Identifier: 74318318

GI number: 74318318

Start: 2372805

End: 2373677

Strand: Reverse

Name: ptxB [H]

Synonym: Tbd_2300

Alternate gene names: 74318318

Gene position: 2373677-2372805 (Counterclockwise)

Preceding gene: 74318319

Following gene: 74318317

Centisome position: 81.58

GC content: 61.86

Gene sequence:

>873_bases
ATGTATGTGAAACACACTTTCTCGGCAAGCGCGCTGGCGCTGGTCCTTTCCCTCGCCGCCGCATCCGCCCAGGCGCGCGA
CATCGTCATGGGCCTCATCCCGGCGGAAAACAATGAAGAAATGGTGCAGAAGTTCGAGCCCATGCGCGCCCACCTGGAGA
AGAAACTGGGCCAGAAGGTGAAGGTGTACACCGCCACCGACTACGCCGGCGTCATCGAAGGCATGAAGAAGAAGCGCGTC
GACGTCGCCTGGTTCGGCCCGCTGTCCTATTACCTGGCCGAGCAGGAAGCCGGCGCCGAGGCCTTCGCCGTCGGCATCCG
CGAGGGCGGCACCACCCATACCTACAAGAGCCTCTTCGTCGCGCCCTGCGACGCCGGCGTCAAGTCGATCAAGGACCTGA
AGGGCAAGAGCGTGGCCTTCGTCGATCCGGCCTCGACATCGGGCGGCCTGATGCCGACCTACATGGTCAAGAAGGAAACC
GGCATGATGCCGCAGCAGTTCTTCGGCAAGTTCACCTACTCCGGCTCGCACGACGCCGCCGAGCTGGCGGTCAAGAACAA
GACCGTGGACGCCGCCGCCGACAACGACATCACCTACGAGAAGATGCTCGCCAAGGGCCTCATCACCAAAGAGACCAACT
GCGTCGTCAAGGAATCCGATCCGCTGCCGGGCTCGCCCCTGGTCTATCGCAAGGATCTGCCGGCGGCGACGAAAAAGAAA
ATCCGGGACGCCATCCTGAACGCCCACAAGGAAATCAAGGTCAGCGGCTACGGCAACCTGAGCCATTACGTGGCGGTCGC
GCCCAAGGATTACCAGATGATCCGCGACATGGTGAAGGACCTGGGCCTGAAGAAGAACGACCTGCTCAAGTAA

Upstream 100 bases:

>100_bases
CCCCGCTCGAATACGCCCTGACCCGGTTCCGCGCCGACCGGATCCAACTGCGCATCAATCCTTGACCTGTCTATCGATCA
ATCTTACGGGAGCAACCGCA

Downstream 100 bases:

>100_bases
GGGCAGAGGGGAAGACAGGCCGCCTTGGCCTGTCTTCCCGGACCAAGGAGAAAATCATGTCCGCGATTTCCATTCAATCC
GTGACCAAGCGCTTCCCGAA

Product: phosphonate-binding periplasmic protein

Products: 3-phospho-serine [Cytoplasm]; phosphate [Cytoplasm]; alkylphosphonate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MYVKHTFSASALALVLSLAAASAQARDIVMGLIPAENNEEMVQKFEPMRAHLEKKLGQKVKVYTATDYAGVIEGMKKKRV
DVAWFGPLSYYLAEQEAGAEAFAVGIREGGTTHTYKSLFVAPCDAGVKSIKDLKGKSVAFVDPASTSGGLMPTYMVKKET
GMMPQQFFGKFTYSGSHDAAELAVKNKTVDAAADNDITYEKMLAKGLITKETNCVVKESDPLPGSPLVYRKDLPAATKKK
IRDAILNAHKEIKVSGYGNLSHYVAVAPKDYQMIRDMVKDLGLKKNDLLK

Sequences:

>Translated_290_residues
MYVKHTFSASALALVLSLAAASAQARDIVMGLIPAENNEEMVQKFEPMRAHLEKKLGQKVKVYTATDYAGVIEGMKKKRV
DVAWFGPLSYYLAEQEAGAEAFAVGIREGGTTHTYKSLFVAPCDAGVKSIKDLKGKSVAFVDPASTSGGLMPTYMVKKET
GMMPQQFFGKFTYSGSHDAAELAVKNKTVDAAADNDITYEKMLAKGLITKETNCVVKESDPLPGSPLVYRKDLPAATKKK
IRDAILNAHKEIKVSGYGNLSHYVAVAPKDYQMIRDMVKDLGLKKNDLLK
>Mature_290_residues
MYVKHTFSASALALVLSLAAASAQARDIVMGLIPAENNEEMVQKFEPMRAHLEKKLGQKVKVYTATDYAGVIEGMKKKRV
DVAWFGPLSYYLAEQEAGAEAFAVGIREGGTTHTYKSLFVAPCDAGVKSIKDLKGKSVAFVDPASTSGGLMPTYMVKKET
GMMPQQFFGKFTYSGSHDAAELAVKNKTVDAAADNDITYEKMLAKGLITKETNCVVKESDPLPGSPLVYRKDLPAATKKK
IRDAILNAHKEIKVSGYGNLSHYVAVAPKDYQMIRDMVKDLGLKKNDLLK

Specific function: Probably forms part of a binding-protein-dependent phosphite transporter. Required for oxidation of phosphite to phosphate [H]

COG id: COG3221

COG function: function code P; ABC-type phosphate/phosphonate transport system, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790543, Length=244, Percent_Identity=34.8360655737705, Blast_Score=134, Evalue=7e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015168
- InterPro:   IPR005770 [H]

Pfam domain/function: PF09084 NMT1 [H]

EC number: NA

Molecular weight: Translated: 31638; Mature: 31638

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYVKHTFSASALALVLSLAAASAQARDIVMGLIPAENNEEMVQKFEPMRAHLEKKLGQKV
CCEECCCCHHHHHHHHHHHHHHHHHCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEE
KVYTATDYAGVIEGMKKKRVDVAWFGPLSYYLAEQEAGAEAFAVGIREGGTTHTYKSLFV
EEEEECCHHHHHHHHHHHCCEEEEECHHHHHHHHHHCCCCEEEEEECCCCCCCHHHEEEE
APCDAGVKSIKDLKGKSVAFVDPASTSGGLMPTYMVKKETGMMPQQFFGKFTYSGSHDAA
ECCHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEEEHHCCCCCHHHHCCCCCCCCCCHH
ELAVKNKTVDAAADNDITYEKMLAKGLITKETNCVVKESDPLPGSPLVYRKDLPAATKKK
HHEEECCEECCCCCCCCHHHHHHHHHCCCCCCCEEEECCCCCCCCCEEEECCCCHHHHHH
IRDAILNAHKEIKVSGYGNLSHYVAVAPKDYQMIRDMVKDLGLKKNDLLK
HHHHHHCCCCEEEEECCCCCCEEEEECCCHHHHHHHHHHHCCCCHHHCCC
>Mature Secondary Structure
MYVKHTFSASALALVLSLAAASAQARDIVMGLIPAENNEEMVQKFEPMRAHLEKKLGQKV
CCEECCCCHHHHHHHHHHHHHHHHHCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEE
KVYTATDYAGVIEGMKKKRVDVAWFGPLSYYLAEQEAGAEAFAVGIREGGTTHTYKSLFV
EEEEECCHHHHHHHHHHHCCEEEEECHHHHHHHHHHCCCCEEEEEECCCCCCCHHHEEEE
APCDAGVKSIKDLKGKSVAFVDPASTSGGLMPTYMVKKETGMMPQQFFGKFTYSGSHDAA
ECCHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEEEHHCCCCCHHHHCCCCCCCCCCHH
ELAVKNKTVDAAADNDITYEKMLAKGLITKETNCVVKESDPLPGSPLVYRKDLPAATKKK
HHEEECCEECCCCCCCCHHHHHHHHHCCCCCCCEEEECCCCCCCCCEEEECCCCHHHHHH
IRDAILNAHKEIKVSGYGNLSHYVAVAPKDYQMIRDMVKDLGLKKNDLLK
HHHHHHCCCCEEEEECCCCCCEEEEECCCHHHHHHHHHHHCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 3-phospho-serine [Periplasm]; phosphite [Periplasm]; H2O; phosphate [Periplasm]; alkylphosphonate [Periplasm] [C]

Specific reaction: H2O + ATP + 3-phospho-serine [Periplasm] = ADP + phosphate + 3-phospho-serine [Cytoplasm] ATP + phosphite [Periplasm] + H2O = phosphite [Cytoplasm] + ADP + phosphate phosphate [Periplasm] + ATP + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] A

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9791102 [H]