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The map label for this gene is phnJ [H]

Identifier: 74318311

GI number: 74318311

Start: 2367345

End: 2368244

Strand: Reverse

Name: phnJ [H]

Synonym: Tbd_2293

Alternate gene names: 74318311

Gene position: 2368244-2367345 (Counterclockwise)

Preceding gene: 74318312

Following gene: 74318310

Centisome position: 81.39

GC content: 66.33

Gene sequence:

>900_bases
ATGAACGCCATGCCCCACACCACCGGCGGCAACGCCTACAACTTCGGCTTCCTCGACGAATACGCCAAGAAGGAAATCCG
CCGCGCCGTCCTCAAGGCCATCGCCATCCCCGGCTACCAGGTGCCCTACGCCTCGCGCGAAATGCCCATGGGACGCGGCT
TCGGCACCGGCGGCCTGCAACTGACGCTGGCCCTGATCGGTCCCGGCGAGGTGGTCAAGGTCATCGACCAGGGTTCGGAC
GATTCGGTGAACGCGGTCAATCTGCGCCGGTTCATCGAAATGACCTGCCCCGGCGTCTCCACCACCACCCGCACCGCGGA
GGCGAGCCTGATCCAGTCGCGCCACCGCATCCCGGAACGGCCGCTGACCCCGACGCAGATCCTCGTGCTGCAAGTGCCTT
ACCCGGACCCGCTGGTGGTGGTCGAGCCGTCCGAGGACAAGCGCAAGATCATGCACGGCGAGGCCGACTATTCGCGCCTG
CTGGTGAAGCTGTACGAGGACATCGTCCAGTTCGACGAGATCACCATCTCGCACCGCTATCCGACGCGCATCAACGGCCA
CTACGTCATCGACCCCTCGCCGATTCCGCGCTGGGACGTGCCCAGGCTCGACCATGCGGCGGCGCTGACCCTGTTCGGCG
CCGGGCGCGAGAAGAAGATTTACGCGGTGCCGCCGCACACTCGCGCGGAGCCGCTGGCGTTTTCCGACGTGCCGTTCCGG
GTCGAGGATTTCCGCGACGAGGCGGGACGCCGCCGCGCCTGCGCGCGCTGCGGCTGCACGGATTCCTTTCTCGACGAGTT
CAGCAGCGATGCCGGCGAGAAGGTCTACCAATGCTCGGACACCGACTGGTGCGACGAAAAACTGCTGCGCAAGGAATCGC
TGCGGGGAGAAGCGGCATGA

Upstream 100 bases:

>100_bases
CCATGGGCTTCGCCTCGCACTACAAGATGCCGCACTACGTCACCTTCCAGTCCGACCTGGACCGGCTGCGCACCACGCAG
CAGAAGTCGGGAGAACCCGC

Downstream 100 bases:

>100_bases
ATAAAATTCTCGAAGTCAGGAATCTGTCGAAAATCTACGGCCCCGGCTGCCCGCACTGCATCGAATCGACCGGGCCGGAA
GCGGACACCAACCTGTGCCC

Product: phosphonate metabolism protein PhnJ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MNAMPHTTGGNAYNFGFLDEYAKKEIRRAVLKAIAIPGYQVPYASREMPMGRGFGTGGLQLTLALIGPGEVVKVIDQGSD
DSVNAVNLRRFIEMTCPGVSTTTRTAEASLIQSRHRIPERPLTPTQILVLQVPYPDPLVVVEPSEDKRKIMHGEADYSRL
LVKLYEDIVQFDEITISHRYPTRINGHYVIDPSPIPRWDVPRLDHAAALTLFGAGREKKIYAVPPHTRAEPLAFSDVPFR
VEDFRDEAGRRRACARCGCTDSFLDEFSSDAGEKVYQCSDTDWCDEKLLRKESLRGEAA

Sequences:

>Translated_299_residues
MNAMPHTTGGNAYNFGFLDEYAKKEIRRAVLKAIAIPGYQVPYASREMPMGRGFGTGGLQLTLALIGPGEVVKVIDQGSD
DSVNAVNLRRFIEMTCPGVSTTTRTAEASLIQSRHRIPERPLTPTQILVLQVPYPDPLVVVEPSEDKRKIMHGEADYSRL
LVKLYEDIVQFDEITISHRYPTRINGHYVIDPSPIPRWDVPRLDHAAALTLFGAGREKKIYAVPPHTRAEPLAFSDVPFR
VEDFRDEAGRRRACARCGCTDSFLDEFSSDAGEKVYQCSDTDWCDEKLLRKESLRGEAA
>Mature_299_residues
MNAMPHTTGGNAYNFGFLDEYAKKEIRRAVLKAIAIPGYQVPYASREMPMGRGFGTGGLQLTLALIGPGEVVKVIDQGSD
DSVNAVNLRRFIEMTCPGVSTTTRTAEASLIQSRHRIPERPLTPTQILVLQVPYPDPLVVVEPSEDKRKIMHGEADYSRL
LVKLYEDIVQFDEITISHRYPTRINGHYVIDPSPIPRWDVPRLDHAAALTLFGAGREKKIYAVPPHTRAEPLAFSDVPFR
VEDFRDEAGRRRACARCGCTDSFLDEFSSDAGEKVYQCSDTDWCDEKLLRKESLRGEAA

Specific function: Belongs to an operon involved in alkylphosphonate uptake and C-P lyase. Exact function not known [H]

COG id: COG3627

COG function: function code P; Uncharacterized enzyme of phosphonate metabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phnJ family [H]

Homologues:

Organism=Escherichia coli, GI1790536, Length=278, Percent_Identity=54.6762589928058, Blast_Score=314, Evalue=5e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010306 [H]

Pfam domain/function: PF06007 PhnJ [H]

EC number: NA

Molecular weight: Translated: 33439; Mature: 33439

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAMPHTTGGNAYNFGFLDEYAKKEIRRAVLKAIAIPGYQVPYASREMPMGRGFGTGGLQ
CCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEE
LTLALIGPGEVVKVIDQGSDDSVNAVNLRRFIEMTCPGVSTTTRTAEASLIQSRHRIPER
EEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCC
PLTPTQILVLQVPYPDPLVVVEPSEDKRKIMHGEADYSRLLVKLYEDIVQFDEITISHRY
CCCCCEEEEEEECCCCCEEEECCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEEECCC
PTRINGHYVIDPSPIPRWDVPRLDHAAALTLFGAGREKKIYAVPPHTRAEPLAFSDVPFR
CCCCCCEEEECCCCCCCCCCCCCCHHHEEEEEECCCCCEEEECCCCCCCCCCEECCCCCH
VEDFRDEAGRRRACARCGCTDSFLDEFSSDAGEKVYQCSDTDWCDEKLLRKESLRGEAA
HHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNAMPHTTGGNAYNFGFLDEYAKKEIRRAVLKAIAIPGYQVPYASREMPMGRGFGTGGLQ
CCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEE
LTLALIGPGEVVKVIDQGSDDSVNAVNLRRFIEMTCPGVSTTTRTAEASLIQSRHRIPER
EEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCC
PLTPTQILVLQVPYPDPLVVVEPSEDKRKIMHGEADYSRLLVKLYEDIVQFDEITISHRY
CCCCCEEEEEEECCCCCEEEECCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEEECCC
PTRINGHYVIDPSPIPRWDVPRLDHAAALTLFGAGREKKIYAVPPHTRAEPLAFSDVPFR
CCCCCCEEEECCCCCCCCCCCCCCHHHEEEEEECCCCCEEEECCCCCCCCCCEECCCCCH
VEDFRDEAGRRRACARCGCTDSFLDEFSSDAGEKVYQCSDTDWCDEKLLRKESLRGEAA
HHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2155230; 1840580; 7610040; 9278503 [H]