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The map label for this gene is nrtB [H]

Identifier: 74318291

GI number: 74318291

Start: 2344592

End: 2345491

Strand: Direct

Name: nrtB [H]

Synonym: Tbd_2273

Alternate gene names: 74318291

Gene position: 2344592-2345491 (Clockwise)

Preceding gene: 74318290

Following gene: 74318292

Centisome position: 80.58

GC content: 66.78

Gene sequence:

>900_bases
ATGCACGCAATCAACGCAAGAAACCAAGCGCGCAGCGCGCTTCCGGCGAAAGCCGAAGTCGTCGCGCTTCCCACGCCGCC
GGCGCGACACGCGCCCTATTTCGAGACCGACGCCGGCCGCCGCCTGCTCGACGGCGGCGCGACGCTGGTGAAGAACGCGA
TTCCGCCGCTGCTTGGCCTGCTGCTTTTCACGGCGCTGTGGGCGCTGGTCGCGAACAGCGGGCAAACGCTTCCGGGGCCG
ACGGAGACCTGGGAGGCGGCACGCGCGCTGTTCGCCGACCCCTTCTACGACAACGGGCCCAACGACCAGGGCATCGGCTG
GAACGTCCTCAACTCGCTCTATCGCGTCGGCGTCGGCTTCGGCGCAGCAGCAGCAGTCGGGATACCGCTCGGCTTCATGA
TCGGCCGCTTCGATTTTCTGAACCGCATGCTCGCGCCGATCATCGGCATCCTGCGCCCGGTCTCTCCGCTCGCGTGGCTG
CCGATCGGCCTGCTCGTGCTGCAGAAGGCCGAGCCGGCGTCGATCTGGGTGATCTTCATCTCGAGCATCTGGCCGATGGT
GCTCAACACCGCTGCCGGCGTGCAGCGCGTGCCGCAGGATTACATGAACGTCGCGCGGGTGTTGAAGCTGTCCGAGTTCC
GCGTGTTCACGCGCATCCTCTTCCCCGCCGTGCTGCCCCACGTGCTGACCGGCGTGCGCCTGTCGATCGGCGTCGCCTGG
CTCGTCATCGTCGCCGCCGAAATGCTGACCGGCGGCGTCGGCATCGGCTTCTGGGTGTGGGACGAATGGAACAACCTCAA
CGTCGCCCACATCATCATCGCGATCTTCATCGTCGGCCTCGTCGGGCTCGCGCTCGAGCAGGGGCTGATGGTGCTGGCGA
AGCGGTTCTCTTACGAGTAG

Upstream 100 bases:

>100_bases
GGCTCGAACCCGCAGGCCTACGCCGACGGCTTCCGAATCAAGGCCTGAGCGAGCGCCGCTTCGATCCGAGTCCGACGCAT
CACCCTTGGAGGACTACCCC

Downstream 100 bases:

>100_bases
GGATCAGGGGTCAGGATGCAGGGATCAGGGGACGTGCGGCCTCCGGCCGCGCCTTCCCATATCCGCGAAGCGACCTCCCC
TGACCCCCGACCCCTGAATC

Product: nitrate transport permease

Products: taurine [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MHAINARNQARSALPAKAEVVALPTPPARHAPYFETDAGRRLLDGGATLVKNAIPPLLGLLLFTALWALVANSGQTLPGP
TETWEAARALFADPFYDNGPNDQGIGWNVLNSLYRVGVGFGAAAAVGIPLGFMIGRFDFLNRMLAPIIGILRPVSPLAWL
PIGLLVLQKAEPASIWVIFISSIWPMVLNTAAGVQRVPQDYMNVARVLKLSEFRVFTRILFPAVLPHVLTGVRLSIGVAW
LVIVAAEMLTGGVGIGFWVWDEWNNLNVAHIIIAIFIVGLVGLALEQGLMVLAKRFSYE

Sequences:

>Translated_299_residues
MHAINARNQARSALPAKAEVVALPTPPARHAPYFETDAGRRLLDGGATLVKNAIPPLLGLLLFTALWALVANSGQTLPGP
TETWEAARALFADPFYDNGPNDQGIGWNVLNSLYRVGVGFGAAAAVGIPLGFMIGRFDFLNRMLAPIIGILRPVSPLAWL
PIGLLVLQKAEPASIWVIFISSIWPMVLNTAAGVQRVPQDYMNVARVLKLSEFRVFTRILFPAVLPHVLTGVRLSIGVAW
LVIVAAEMLTGGVGIGFWVWDEWNNLNVAHIIIAIFIVGLVGLALEQGLMVLAKRFSYE
>Mature_299_residues
MHAINARNQARSALPAKAEVVALPTPPARHAPYFETDAGRRLLDGGATLVKNAIPPLLGLLLFTALWALVANSGQTLPGP
TETWEAARALFADPFYDNGPNDQGIGWNVLNSLYRVGVGFGAAAAVGIPLGFMIGRFDFLNRMLAPIIGILRPVSPLAWL
PIGLLVLQKAEPASIWVIFISSIWPMVLNTAAGVQRVPQDYMNVARVLKLSEFRVFTRILFPAVLPHVLTGVRLSIGVAW
LVIVAAEMLTGGVGIGFWVWDEWNNLNVAHIIIAIFIVGLVGLALEQGLMVLAKRFSYE

Specific function: Probably part of a high-affinity binding-protein- dependent transport system for nitrate; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0600

COG function: function code P; ABC-type nitrate/sulfonate/bicarbonate transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1786564, Length=221, Percent_Identity=31.2217194570136, Blast_Score=94, Evalue=8e-21,
Organism=Escherichia coli, GI87081802, Length=142, Percent_Identity=28.8732394366197, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR005889 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32372; Mature: 32372

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHAINARNQARSALPAKAEVVALPTPPARHAPYFETDAGRRLLDGGATLVKNAIPPLLGL
CCCCCCCHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHH
LLFTALWALVANSGQTLPGPTETWEAARALFADPFYDNGPNDQGIGWNVLNSLYRVGVGF
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCC
GAAAAVGIPLGFMIGRFDFLNRMLAPIIGILRPVSPLAWLPIGLLVLQKAEPASIWVIFI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHH
SSIWPMVLNTAAGVQRVPQDYMNVARVLKLSEFRVFTRILFPAVLPHVLTGVRLSIGVAW
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVIVAAEMLTGGVGIGFWVWDEWNNLNVAHIIIAIFIVGLVGLALEQGLMVLAKRFSYE
HHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MHAINARNQARSALPAKAEVVALPTPPARHAPYFETDAGRRLLDGGATLVKNAIPPLLGL
CCCCCCCHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHH
LLFTALWALVANSGQTLPGPTETWEAARALFADPFYDNGPNDQGIGWNVLNSLYRVGVGF
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCC
GAAAAVGIPLGFMIGRFDFLNRMLAPIIGILRPVSPLAWLPIGLLVLQKAEPASIWVIFI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHH
SSIWPMVLNTAAGVQRVPQDYMNVARVLKLSEFRVFTRILFPAVLPHVLTGVRLSIGVAW
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVIVAAEMLTGGVGIGFWVWDEWNNLNVAHIIIAIFIVGLVGLALEQGLMVLAKRFSYE
HHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: taurine [Periplasm]; ATP; H2O [C]

Specific reaction: taurine [Periplasm] + ATP + H2O = taurine [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]