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The map label for this gene is mak [H]

Identifier: 74318234

GI number: 74318234

Start: 2294222

End: 2295211

Strand: Direct

Name: mak [H]

Synonym: Tbd_2216

Alternate gene names: 74318234

Gene position: 2294222-2295211 (Clockwise)

Preceding gene: 74318216

Following gene: 74318239

Centisome position: 78.84

GC content: 70.1

Gene sequence:

>990_bases
GTGGCCCCTTCCCTCCGTTTCGGCATCGACCTCGGCGGCAGCAAAATCGAACTCATCGCACTCGACCGCGACGGCCGGGA
AATCCTGCGTCGGCGCGTCCCGACGCCGCAGGGCGACTACCCCGCGACGGTCGCCGCGATCGCCGCGCTGGTCGGCCACG
CCGAATCGGGCTTGGGACAGCAGGGCAGCGTCGGCGTCGGAACCCCGGGCGCGGTCTCACCGGCGAGCGGCCGCATGAAG
AACTGCAACTCGACCTGCCTCAACGGCCAGCCGCTCAGGGAGGATCTCGAACGCGCGCTCGGCCGCGAGGTCCGGGTCGC
CAACGACGCCAATTGCCTGGCGCTGTCGGAAGCGACCGACGGTTCGGCGGCCGGGGCCGAGAGCGTGTTCGGCGTAATCC
TCGGTACCGGCGTCGGCGGCGGCGTGGTCGTGCACGGCCGGCTGCTCCAGGGCGCCAATGCCATCGCCGGGGAATGGGGG
CACTCACCGCTGCCCTATTTCCAGTTCGCCGGCGCGCAGGCCGACCGCGCCATGACTGGACACCATCCTGCGACCGGCGA
AGCGATCGTCCACCCCTGGCCGCAACCGCGCGAACTGGACGCCGCCCCCGCCTGCTACTGCGGCAAGAAGGGCTGCATCG
AAACCTGGCTCTCGGGACCCGGTCTCGCCGCCGACCACGTGCGCTACGGCGGCGAGGACCTGCCCGCGCACGAGATCGTG
CAACTGGCGAACGCGGGCTACGGCCCATGCAGCGCGACGCTCGCGCGCTACGAAGAAAGACTTGCGCGCGCGCTCGCCGG
CGTCATCAACCTCGTCGACCCCGACGTGATCGTGCTCGGTGGCGGGCTCTCCAATATCGCCCGGCTGTATGACACCGTGC
CGCGTCTATGGCCGCGTTACGTGTTTTCCGATCGCGTCGACACCAAGTTCGTGCCGCCGAAATACGGCGATTCGAGCGGC
GTGCGCGGCGCGGCCTGGTTATGGGAGTAG

Upstream 100 bases:

>100_bases
TTGGCATCCTGGCGACCCTTGCGGTGCTGGATGTTGGCCCACTTCGAATGTCCTGCCATGTCGATTCCAACGCAATTTGA
ATAGCCGGAGATTTTACACC

Downstream 100 bases:

>100_bases
CCGGTTTAACTCGGTAGCATTGCGGCCGGCGCCCCAACCGCCCCGCGCCGCGCCGAGATTTCGCGCTCGGCGGTTTCGAT
CATGCCTTTTAACAGTTGGG

Product: N-acetylglucosamine kinase

Products: NA

Alternate protein names: D-fructose kinase; Manno(fructo)kinase [H]

Number of amino acids: Translated: 329; Mature: 328

Protein sequence:

>329_residues
MAPSLRFGIDLGGSKIELIALDRDGREILRRRVPTPQGDYPATVAAIAALVGHAESGLGQQGSVGVGTPGAVSPASGRMK
NCNSTCLNGQPLREDLERALGREVRVANDANCLALSEATDGSAAGAESVFGVILGTGVGGGVVVHGRLLQGANAIAGEWG
HSPLPYFQFAGAQADRAMTGHHPATGEAIVHPWPQPRELDAAPACYCGKKGCIETWLSGPGLAADHVRYGGEDLPAHEIV
QLANAGYGPCSATLARYEERLARALAGVINLVDPDVIVLGGGLSNIARLYDTVPRLWPRYVFSDRVDTKFVPPKYGDSSG
VRGAAWLWE

Sequences:

>Translated_329_residues
MAPSLRFGIDLGGSKIELIALDRDGREILRRRVPTPQGDYPATVAAIAALVGHAESGLGQQGSVGVGTPGAVSPASGRMK
NCNSTCLNGQPLREDLERALGREVRVANDANCLALSEATDGSAAGAESVFGVILGTGVGGGVVVHGRLLQGANAIAGEWG
HSPLPYFQFAGAQADRAMTGHHPATGEAIVHPWPQPRELDAAPACYCGKKGCIETWLSGPGLAADHVRYGGEDLPAHEIV
QLANAGYGPCSATLARYEERLARALAGVINLVDPDVIVLGGGLSNIARLYDTVPRLWPRYVFSDRVDTKFVPPKYGDSSG
VRGAAWLWE
>Mature_328_residues
APSLRFGIDLGGSKIELIALDRDGREILRRRVPTPQGDYPATVAAIAALVGHAESGLGQQGSVGVGTPGAVSPASGRMKN
CNSTCLNGQPLREDLERALGREVRVANDANCLALSEATDGSAAGAESVFGVILGTGVGGGVVVHGRLLQGANAIAGEWGH
SPLPYFQFAGAQADRAMTGHHPATGEAIVHPWPQPRELDAAPACYCGKKGCIETWLSGPGLAADHVRYGGEDLPAHEIVQ
LANAGYGPCSATLARYEERLARALAGVINLVDPDVIVLGGGLSNIARLYDTVPRLWPRYVFSDRVDTKFVPPKYGDSSGV
RGAAWLWE

Specific function: Catalyzes the phosphorylation of fructose to fructose-6- P. Has also low level glucokinase activity in vitro. Is not able to phosphorylate D-ribose, D-mannitol, D-sorbitol, inositol, and L-threonine [H]

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family [H]

Homologues:

Organism=Escherichia coli, GI87081733, Length=324, Percent_Identity=50.6172839506173, Blast_Score=312, Evalue=2e-86,
Organism=Escherichia coli, GI1787363, Length=323, Percent_Identity=36.8421052631579, Blast_Score=195, Evalue=4e-51,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000600 [H]

Pfam domain/function: PF00480 ROK [H]

EC number: =2.7.1.4 [H]

Molecular weight: Translated: 34403; Mature: 34272

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS01125 ROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPSLRFGIDLGGSKIELIALDRDGREILRRRVPTPQGDYPATVAAIAALVGHAESGLGQ
CCCCCEEEEECCCCEEEEEEECCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCC
QGSVGVGTPGAVSPASGRMKNCNSTCLNGQPLREDLERALGREVRVANDANCLALSEATD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCEEEECCCCCEEEEECCCC
GSAAGAESVFGVILGTGVGGGVVVHGRLLQGANAIAGEWGHSPLPYFQFAGAQADRAMTG
CCCCCHHHHHHHHHCCCCCCCEEEEEEHHCCCCHHCCCCCCCCCCHHHCCCCCCCCCCCC
HHPATGEAIVHPWPQPRELDAAPACYCGKKGCIETWLSGPGLAADHVRYGGEDLPAHEIV
CCCCCCCEEECCCCCCCCCCCCCCHHCCCCHHHHHHHCCCCCCHHHHHCCCCCCCHHHHH
QLANAGYGPCSATLARYEERLARALAGVINLVDPDVIVLGGGLSNIARLYDTVPRLWPRY
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHH
VFSDRVDTKFVPPKYGDSSGVRGAAWLWE
HHCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
APSLRFGIDLGGSKIELIALDRDGREILRRRVPTPQGDYPATVAAIAALVGHAESGLGQ
CCCCEEEEECCCCEEEEEEECCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCC
QGSVGVGTPGAVSPASGRMKNCNSTCLNGQPLREDLERALGREVRVANDANCLALSEATD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCEEEECCCCCEEEEECCCC
GSAAGAESVFGVILGTGVGGGVVVHGRLLQGANAIAGEWGHSPLPYFQFAGAQADRAMTG
CCCCCHHHHHHHHHCCCCCCCEEEEEEHHCCCCHHCCCCCCCCCCHHHCCCCCCCCCCCC
HHPATGEAIVHPWPQPRELDAAPACYCGKKGCIETWLSGPGLAADHVRYGGEDLPAHEIV
CCCCCCCEEECCCCCCCCCCCCCCHHCCCCHHHHHHHCCCCCCHHHHHCCCCCCCHHHHH
QLANAGYGPCSATLARYEERLARALAGVINLVDPDVIVLGGGLSNIARLYDTVPRLWPRY
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHH
VFSDRVDTKFVPPKYGDSSGVRGAAWLWE
HHCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8807285; 9278503; 1744033 [H]