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The map label for this gene is pleD [H]

Identifier: 74318230

GI number: 74318230

Start: 2290719

End: 2292224

Strand: Reverse

Name: pleD [H]

Synonym: Tbd_2212

Alternate gene names: 74318230

Gene position: 2292224-2290719 (Counterclockwise)

Preceding gene: 74318231

Following gene: 74318229

Centisome position: 78.78

GC content: 68.66

Gene sequence:

>1506_bases
ATGCCTGCTCATGCGCCCGCCCGAGCCGCGACCGCCTCCTTGCGGCGCCATTTCGTATGGCTGCTGCTTGGGGTCGTGCT
GATGCTGGTTGGGGGGGTGGGCCTGAGCCTGTGGTTCAGCCTGAAGGCCGAACGCCAGAACCGGGATGGGTTGCTGCGAT
TACACGCCGAAAGCGCGCGGGATGGCGTGAAGCGGCGTCTTGACCACTATCGCAGCCTGGTCGACAAGCTGGCGCGCGAT
CCCGAGCTGGAGGACCTGCTGCGCTTCGGTGGCTCGCCCGCCCCGCAGGACTGGGTCCAGTCGCGCCAGCCCCTGATCCC
GGACTTGCACGGCTTGACCTTGCTCACGCCCGAGGGTGAGGTGCTGGGCGACGGGACGACCTTGCGGATCGGCCCGGACT
GCCGCGCGGAACTGCGGTCCCGACAACCGCTTCGCGGCAGGAGACTGCACCTCCAGCGGGGCGTCGGCGGCCGCGACCAT
CTCGACCTGGTATCCGCCGTGCGCGGGGTCGATAACGAGCTGCTGGGCGGTGTTCTTCTGAGTATGCGACTGGATGCCCT
GAAGCGGATTCTCGAAGAGGCCGCCCATCCCGGGCAGACCCTCACTCTGCTCGACGCCGCCGGCAAGACCGTCGTCAGCA
ACGGCGAGGTGGCTGACGCCGCCCACGAGGTCCGACTGGACGTGGGGGAAGGTGGCTGGACGCTGGTCGGGCGGATGCCG
GGTCAGACCCTGACGCACAGCGGCAAGGCGCAGCTCATCGCCGGTGGCGCGACCCTGCTGGCCGTTCTGCTGCTACTGCA
GGCGAGCATGCTGCACCTGCGCCGGGCGATGCTGCGGGATATCGACGCGACCCGCGACGCCCTGACCGCCTTGGCGCATG
GCGAGCCGGTGCAGGCCATCGTGCCGCACTACGTCGAATTCGCGCCGACGGCTGGCGAGATCAACCTGATCGCCCTGCAG
CTTCAGGCGCAGCGCGAGCAGCTGGAGCATCTGAGCCTGACCGATCCGCTGACCGGCTTGCCCAATCGCCGCGCATTCGA
GACGCGGTTCCCGCAGGCGCTGGGATTCGCCGGGCGCGGCCTTCGCATTGCGCTGGTGCTGCTCGACATCGATTATTTCA
AGCGCATCAATGACAGCTTCGGCCATGGTGTCGGCGACCAGGTGCTGCTCGCGCTGGCGCAGACGCTCAAGGCCCTGACC
CGCCGTGCCGATCTGCCGGCGCGGCTGGCAGGCGACGAGTTCGTCATCCTGCTGGCCGATCTCGATACGGCCGGCGTGGC
CTTGTGGTACCGGCGCCTGGCCGACCATTTCCGCAGCGAGCTCGCTGCCTTGGGCCTGGATATCCAGACCGGCATCAGCG
CTGGGCAGACGTGGCTGCGCCGGACGTCGCGCGACACTATGAACGACGCGCTGGGCCGTGCCGACCGGGCCCTGTATCAA
GCCAAGGCCAGCGGGCGCAATCAGATCGTCATCGACGCGCCGCTGGACGAAACCGGCGCAGGGTAG

Upstream 100 bases:

>100_bases
AGCGCGAGACGGCCGCGACCGTGCGCGAGCTCCCCGCCGGTCTGGCGGTCGGCGAGGCCATCCGCCAGGCGCTGCGCGCC
CTGTCGCGGGCCTGATCGGC

Downstream 100 bases:

>100_bases
AATTCCCGCATGCCCGCTGTCCGCTGCGTTCCATGATCGAAACCGACCGCCTTATCGTACCGACCGCCGTCGGCCCGCAG
GAAGAGCAGATCGAGCGCGC

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 501; Mature: 500

Protein sequence:

>501_residues
MPAHAPARAATASLRRHFVWLLLGVVLMLVGGVGLSLWFSLKAERQNRDGLLRLHAESARDGVKRRLDHYRSLVDKLARD
PELEDLLRFGGSPAPQDWVQSRQPLIPDLHGLTLLTPEGEVLGDGTTLRIGPDCRAELRSRQPLRGRRLHLQRGVGGRDH
LDLVSAVRGVDNELLGGVLLSMRLDALKRILEEAAHPGQTLTLLDAAGKTVVSNGEVADAAHEVRLDVGEGGWTLVGRMP
GQTLTHSGKAQLIAGGATLLAVLLLLQASMLHLRRAMLRDIDATRDALTALAHGEPVQAIVPHYVEFAPTAGEINLIALQ
LQAQREQLEHLSLTDPLTGLPNRRAFETRFPQALGFAGRGLRIALVLLDIDYFKRINDSFGHGVGDQVLLALAQTLKALT
RRADLPARLAGDEFVILLADLDTAGVALWYRRLADHFRSELAALGLDIQTGISAGQTWLRRTSRDTMNDALGRADRALYQ
AKASGRNQIVIDAPLDETGAG

Sequences:

>Translated_501_residues
MPAHAPARAATASLRRHFVWLLLGVVLMLVGGVGLSLWFSLKAERQNRDGLLRLHAESARDGVKRRLDHYRSLVDKLARD
PELEDLLRFGGSPAPQDWVQSRQPLIPDLHGLTLLTPEGEVLGDGTTLRIGPDCRAELRSRQPLRGRRLHLQRGVGGRDH
LDLVSAVRGVDNELLGGVLLSMRLDALKRILEEAAHPGQTLTLLDAAGKTVVSNGEVADAAHEVRLDVGEGGWTLVGRMP
GQTLTHSGKAQLIAGGATLLAVLLLLQASMLHLRRAMLRDIDATRDALTALAHGEPVQAIVPHYVEFAPTAGEINLIALQ
LQAQREQLEHLSLTDPLTGLPNRRAFETRFPQALGFAGRGLRIALVLLDIDYFKRINDSFGHGVGDQVLLALAQTLKALT
RRADLPARLAGDEFVILLADLDTAGVALWYRRLADHFRSELAALGLDIQTGISAGQTWLRRTSRDTMNDALGRADRALYQ
AKASGRNQIVIDAPLDETGAG
>Mature_500_residues
PAHAPARAATASLRRHFVWLLLGVVLMLVGGVGLSLWFSLKAERQNRDGLLRLHAESARDGVKRRLDHYRSLVDKLARDP
ELEDLLRFGGSPAPQDWVQSRQPLIPDLHGLTLLTPEGEVLGDGTTLRIGPDCRAELRSRQPLRGRRLHLQRGVGGRDHL
DLVSAVRGVDNELLGGVLLSMRLDALKRILEEAAHPGQTLTLLDAAGKTVVSNGEVADAAHEVRLDVGEGGWTLVGRMPG
QTLTHSGKAQLIAGGATLLAVLLLLQASMLHLRRAMLRDIDATRDALTALAHGEPVQAIVPHYVEFAPTAGEINLIALQL
QAQREQLEHLSLTDPLTGLPNRRAFETRFPQALGFAGRGLRIALVLLDIDYFKRINDSFGHGVGDQVLLALAQTLKALTR
RADLPARLAGDEFVILLADLDTAGVALWYRRLADHFRSELAALGLDIQTGISAGQTWLRRTSRDTMNDALGRADRALYQA
KASGRNQIVIDAPLDETGAG

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI87081881, Length=168, Percent_Identity=36.9047619047619, Blast_Score=96, Evalue=7e-21,
Organism=Escherichia coli, GI1787816, Length=166, Percent_Identity=34.9397590361446, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1787262, Length=164, Percent_Identity=32.9268292682927, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1786584, Length=177, Percent_Identity=31.638418079096, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1787541, Length=170, Percent_Identity=30, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI145693134, Length=186, Percent_Identity=32.258064516129, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI87081977, Length=165, Percent_Identity=32.1212121212121, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1788381, Length=174, Percent_Identity=33.9080459770115, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI87081974, Length=154, Percent_Identity=31.8181818181818, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1788956, Length=119, Percent_Identity=37.8151260504202, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1787802, Length=132, Percent_Identity=31.8181818181818, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1788085, Length=180, Percent_Identity=32.7777777777778, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI87082007, Length=158, Percent_Identity=34.1772151898734, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 54494; Mature: 54362

Theoretical pI: Translated: 8.52; Mature: 8.52

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAHAPARAATASLRRHFVWLLLGVVLMLVGGVGLSLWFSLKAERQNRDGLLRLHAESAR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEEEHHHHH
DGVKRRLDHYRSLVDKLARDPELEDLLRFGGSPAPQDWVQSRQPLIPDLHGLTLLTPEGE
HHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCEEECCCCC
VLGDGTTLRIGPDCRAELRSRQPLRGRRLHLQRGVGGRDHLDLVSAVRGVDNELLGGVLL
EECCCCEEEECCCHHHHHHCCCCCCCCHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHH
SMRLDALKRILEEAAHPGQTLTLLDAAGKTVVSNGEVADAAHEVRLDVGEGGWTLVGRMP
HHHHHHHHHHHHHHCCCCCEEEEEECCCCEEECCCCHHHCHHHEEEEECCCCEEEEECCC
GQTLTHSGKAQLIAGGATLLAVLLLLQASMLHLRRAMLRDIDATRDALTALAHGEPVQAI
CCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
VPHYVEFAPTAGEINLIALQLQAQREQLEHLSLTDPLTGLPNRRAFETRFPQALGFAGRG
HHHHHHCCCCCCCEEEEEEEEHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCHHHCCCCCC
LRIALVLLDIDYFKRINDSFGHGVGDQVLLALAQTLKALTRRADLPARLAGDEFVILLAD
CEEEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEECCCCEEEEEEC
LDTAGVALWYRRLADHFRSELAALGLDIQTGISAGQTWLRRTSRDTMNDALGRADRALYQ
CCHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKASGRNQIVIDAPLDETGAG
HHCCCCCEEEEECCCCCCCCC
>Mature Secondary Structure 
PAHAPARAATASLRRHFVWLLLGVVLMLVGGVGLSLWFSLKAERQNRDGLLRLHAESAR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEEEHHHHH
DGVKRRLDHYRSLVDKLARDPELEDLLRFGGSPAPQDWVQSRQPLIPDLHGLTLLTPEGE
HHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCEEECCCCC
VLGDGTTLRIGPDCRAELRSRQPLRGRRLHLQRGVGGRDHLDLVSAVRGVDNELLGGVLL
EECCCCEEEECCCHHHHHHCCCCCCCCHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHH
SMRLDALKRILEEAAHPGQTLTLLDAAGKTVVSNGEVADAAHEVRLDVGEGGWTLVGRMP
HHHHHHHHHHHHHHCCCCCEEEEEECCCCEEECCCCHHHCHHHEEEEECCCCEEEEECCC
GQTLTHSGKAQLIAGGATLLAVLLLLQASMLHLRRAMLRDIDATRDALTALAHGEPVQAI
CCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
VPHYVEFAPTAGEINLIALQLQAQREQLEHLSLTDPLTGLPNRRAFETRFPQALGFAGRG
HHHHHHCCCCCCCEEEEEEEEHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCHHHCCCCCC
LRIALVLLDIDYFKRINDSFGHGVGDQVLLALAQTLKALTRRADLPARLAGDEFVILLAD
CEEEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEECCCCEEEEEEC
LDTAGVALWYRRLADHFRSELAALGLDIQTGISAGQTWLRRTSRDTMNDALGRADRALYQ
CCHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKASGRNQIVIDAPLDETGAG
HHCCCCCEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA