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The map label for this gene is glyA

Identifier: 74318183

GI number: 74318183

Start: 2250172

End: 2251416

Strand: Direct

Name: glyA

Synonym: Tbd_2165

Alternate gene names: 74318183

Gene position: 2250172-2251416 (Clockwise)

Preceding gene: 74318180

Following gene: 74318184

Centisome position: 77.33

GC content: 64.74

Gene sequence:

>1245_bases
ATGTTCAACCGCCAAGACACCCTCGCCAAAACCGATCCCGACCTGTGGGCCGCGATCCAGCAGGAAGACCGCCGCCAGCA
GGATCACATCGAGCTGATCGCGTCGGAGAACTACACGAGCCCGGCGGTGATGCAGGCGCAGGGCTCGCAGCTCACCAACA
AGTACGCCGAGGGCTATCCCGGCAAGCGCTACTACGGCGGCTGCGAATACGTCGACATCGTCGAACAGCTCGCGATCGAT
CGCGTCAAGGCGCTGTTCGGCGCCGAGGCGGCGAACGTACAGCCGAACTCGGGCTCGCAGGCGAACCAGGCCGTGTTCAT
GGCCTTCCTGAAGCCCGGCGACACGATCATGGGCATGAGCCTCGCCGAGGGCGGCCACCTGACGCACGGCATGGCGCTCA
ACATGAGCGGCAAATGGTTCAACGTCGTCGCCTACGGCCTCAACGAAAAGGAAGAGATCGACTACGAAGCGATGGAACGG
CTCGCGCGCGAGCACAAGCCGAAACTGATCATCGCCGGCGCCTCGGCCTACGCGCTCAGGATCGACTTCGAGCGCTTCGC
CAAGATCGCGAAGGAAATCGGTGCGATCTTCATGGTCGACATGGCGCACTACGCGGGCCTGATCGCCGCCGGCCTCTACC
CCAACCCGGTGCCGCACGCCGACGTCGTCACTTCGACCACGCACAAGACGCTGCGCGGCCCGCGCGGCGGCATCATCCTG
ATGAAGGCCGAACACGAGAAGGCGATCAACAGCGCGATCTTTCCCGGCCTGCAGGGCGGCCCGCTCATGCACGTCATCGC
CGGCAAGGCGACCGCGTTCAAGGAAGCGGCGACCAAGGACTTCAAGCGCTACCAGGAACAGGTGATCGATAACGCGCTCG
TGATGTGCAAGGTACTCGTCGAGCGCGGCCTCCGGATCATCTCGGGCCGCACCGAGAGCCACGTGTTCCTCGTCGACCTG
CGCGCGAAGAACCTGACCGGCAAGGAAGCCGAGGCGCTCCTGGGCCGCGCGCACATCACCGTCAACAAGAACGCGATCCC
CAACGATCCGCAGAAGCCCTTTGTCACGAGCGGCATCCGCATCGGCACGCCGGCGATGACGACGCGCGGCTTCACCGAAC
TCGAAGCCGAGCAGCTCGCCCACCTGATCGCCGACGTCCTTGACGCGCCGAACGACGAGGCCGTCGTCGAGCGTGTGAAG
GGTGAAGTGGCGAAGCTGACGGCGAAGTTTCCGGTTTACGGCTGA

Upstream 100 bases:

>100_bases
TTTGCATGCTTGGTCCCGGTTTTTCGCCGATTATACGAGCCGCTTCGACGAAACCGCCGCGTGATAAAATCGCCGCCTCT
CAAGCCCCCCCCCCTCTGCC

Downstream 100 bases:

>100_bases
GGCCCAGGGGTCCGGGGTCGGGATTCGGGGATCAGGGAATGTTGCTTCGCGTTTCCTTGAATATGAACGCGGCCATGCCG
CGCAAAACCCTCTGACCCCT

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MFNRQDTLAKTDPDLWAAIQQEDRRQQDHIELIASENYTSPAVMQAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAID
RVKALFGAEAANVQPNSGSQANQAVFMAFLKPGDTIMGMSLAEGGHLTHGMALNMSGKWFNVVAYGLNEKEEIDYEAMER
LAREHKPKLIIAGASAYALRIDFERFAKIAKEIGAIFMVDMAHYAGLIAAGLYPNPVPHADVVTSTTHKTLRGPRGGIIL
MKAEHEKAINSAIFPGLQGGPLMHVIAGKATAFKEAATKDFKRYQEQVIDNALVMCKVLVERGLRIISGRTESHVFLVDL
RAKNLTGKEAEALLGRAHITVNKNAIPNDPQKPFVTSGIRIGTPAMTTRGFTELEAEQLAHLIADVLDAPNDEAVVERVK
GEVAKLTAKFPVYG

Sequences:

>Translated_414_residues
MFNRQDTLAKTDPDLWAAIQQEDRRQQDHIELIASENYTSPAVMQAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAID
RVKALFGAEAANVQPNSGSQANQAVFMAFLKPGDTIMGMSLAEGGHLTHGMALNMSGKWFNVVAYGLNEKEEIDYEAMER
LAREHKPKLIIAGASAYALRIDFERFAKIAKEIGAIFMVDMAHYAGLIAAGLYPNPVPHADVVTSTTHKTLRGPRGGIIL
MKAEHEKAINSAIFPGLQGGPLMHVIAGKATAFKEAATKDFKRYQEQVIDNALVMCKVLVERGLRIISGRTESHVFLVDL
RAKNLTGKEAEALLGRAHITVNKNAIPNDPQKPFVTSGIRIGTPAMTTRGFTELEAEQLAHLIADVLDAPNDEAVVERVK
GEVAKLTAKFPVYG
>Mature_414_residues
MFNRQDTLAKTDPDLWAAIQQEDRRQQDHIELIASENYTSPAVMQAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAID
RVKALFGAEAANVQPNSGSQANQAVFMAFLKPGDTIMGMSLAEGGHLTHGMALNMSGKWFNVVAYGLNEKEEIDYEAMER
LAREHKPKLIIAGASAYALRIDFERFAKIAKEIGAIFMVDMAHYAGLIAAGLYPNPVPHADVVTSTTHKTLRGPRGGIIL
MKAEHEKAINSAIFPGLQGGPLMHVIAGKATAFKEAATKDFKRYQEQVIDNALVMCKVLVERGLRIISGRTESHVFLVDL
RAKNLTGKEAEALLGRAHITVNKNAIPNDPQKPFVTSGIRIGTPAMTTRGFTELEAEQLAHLIADVLDAPNDEAVVERVK
GEVAKLTAKFPVYG

Specific function: Interconversion of serine and glycine

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family

Homologues:

Organism=Homo sapiens, GI261862352, Length=404, Percent_Identity=45.2970297029703, Blast_Score=339, Evalue=3e-93,
Organism=Homo sapiens, GI261862350, Length=404, Percent_Identity=45.2970297029703, Blast_Score=339, Evalue=3e-93,
Organism=Homo sapiens, GI261862348, Length=404, Percent_Identity=45.2970297029703, Blast_Score=339, Evalue=3e-93,
Organism=Homo sapiens, GI19923315, Length=404, Percent_Identity=45.2970297029703, Blast_Score=339, Evalue=3e-93,
Organism=Homo sapiens, GI261862346, Length=403, Percent_Identity=44.4168734491315, Blast_Score=329, Evalue=3e-90,
Organism=Homo sapiens, GI22547186, Length=401, Percent_Identity=44.3890274314214, Blast_Score=313, Evalue=1e-85,
Organism=Homo sapiens, GI22547189, Length=386, Percent_Identity=41.9689119170984, Blast_Score=279, Evalue=3e-75,
Organism=Escherichia coli, GI1788902, Length=416, Percent_Identity=65.625, Blast_Score=555, Evalue=1e-159,
Organism=Caenorhabditis elegans, GI25144732, Length=402, Percent_Identity=45.0248756218905, Blast_Score=341, Evalue=5e-94,
Organism=Caenorhabditis elegans, GI25144729, Length=402, Percent_Identity=45.0248756218905, Blast_Score=340, Evalue=6e-94,
Organism=Saccharomyces cerevisiae, GI6319739, Length=457, Percent_Identity=40.2625820568928, Blast_Score=338, Evalue=1e-93,
Organism=Saccharomyces cerevisiae, GI6323087, Length=401, Percent_Identity=43.8902743142145, Blast_Score=324, Evalue=1e-89,
Organism=Drosophila melanogaster, GI24640005, Length=457, Percent_Identity=43.3260393873085, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI221329721, Length=457, Percent_Identity=43.3260393873085, Blast_Score=362, Evalue=1e-100,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): GLYA_THIDA (Q3SGX5)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_315923.1
- ProteinModelPortal:   Q3SGX5
- SMR:   Q3SGX5
- STRING:   Q3SGX5
- GeneID:   3671946
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_2165
- NMPDR:   fig|292415.3.peg.1700
- eggNOG:   COG0112
- HOGENOM:   HBG301263
- OMA:   LDAKEEI
- PhylomeDB:   Q3SGX5
- ProtClustDB:   PRK00011
- BioCyc:   TDEN292415:TBD_2165-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00051_B
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11680
- PIRSF:   PIRSF000412

Pfam domain/function: PF00464 SHMT; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.1.2.1

Molecular weight: Translated: 45291; Mature: 45291

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNRQDTLAKTDPDLWAAIQQEDRRQQDHIELIASENYTSPAVMQAQGSQLTNKYAEGYP
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEEECCCCCCCHHEECCCHHHHHHHHCCCC
GKRYYGGCEYVDIVEQLAIDRVKALFGAEAANVQPNSGSQANQAVFMAFLKPGDTIMGMS
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEE
LAEGGHLTHGMALNMSGKWFNVVAYGLNEKEEIDYEAMERLAREHKPKLIIAGASAYALR
ECCCCCEECCEEEECCCCEEEEEEECCCCHHCCCHHHHHHHHHHCCCCEEEECCCEEEEE
IDFERFAKIAKEIGAIFMVDMAHYAGLIAAGLYPNPVPHADVVTSTTHKTLRGPRGGIIL
ECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCHHHHHCCCCCCEEE
MKAEHEKAINSAIFPGLQGGPLMHVIAGKATAFKEAATKDFKRYQEQVIDNALVMCKVLV
EECCHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERGLRIISGRTESHVFLVDLRAKNLTGKEAEALLGRAHITVNKNAIPNDPQKPFVTSGIR
HCCCEEECCCCCCEEEEEEEECCCCCCHHHHHHHHHEEEEECCCCCCCCCCCCCEECCEE
IGTPAMTTRGFTELEAEQLAHLIADVLDAPNDEAVVERVKGEVAKLTAKFPVYG
ECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEECCCCCC
>Mature Secondary Structure
MFNRQDTLAKTDPDLWAAIQQEDRRQQDHIELIASENYTSPAVMQAQGSQLTNKYAEGYP
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEEECCCCCCCHHEECCCHHHHHHHHCCCC
GKRYYGGCEYVDIVEQLAIDRVKALFGAEAANVQPNSGSQANQAVFMAFLKPGDTIMGMS
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEE
LAEGGHLTHGMALNMSGKWFNVVAYGLNEKEEIDYEAMERLAREHKPKLIIAGASAYALR
ECCCCCEECCEEEECCCCEEEEEEECCCCHHCCCHHHHHHHHHHCCCCEEEECCCEEEEE
IDFERFAKIAKEIGAIFMVDMAHYAGLIAAGLYPNPVPHADVVTSTTHKTLRGPRGGIIL
ECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCHHHHHCCCCCCEEE
MKAEHEKAINSAIFPGLQGGPLMHVIAGKATAFKEAATKDFKRYQEQVIDNALVMCKVLV
EECCHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERGLRIISGRTESHVFLVDLRAKNLTGKEAEALLGRAHITVNKNAIPNDPQKPFVTSGIR
HCCCEEECCCCCCEEEEEEEECCCCCCHHHHHHHHHEEEEECCCCCCCCCCCCCEECCEE
IGTPAMTTRGFTELEAEQLAHLIADVLDAPNDEAVVERVKGEVAKLTAKFPVYG
ECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA