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The map label for this gene is ilvE [H]

Identifier: 74318152

GI number: 74318152

Start: 2220861

End: 2221784

Strand: Direct

Name: ilvE [H]

Synonym: Tbd_2134

Alternate gene names: 74318152

Gene position: 2220861-2221784 (Clockwise)

Preceding gene: 74318150

Following gene: 74318153

Centisome position: 76.32

GC content: 63.53

Gene sequence:

>924_bases
ATGTCCATGGCCGACCGCGACGGCGTCATATGGTACGACGGCAAACTCGTACCATGGCGCGACGCTACCACCCACGTCCT
CACCCATACCCTGCACTACGGCATGGGTGTCTTCGAGGGCGTGCGCGCCTACAAGGCCGAACAGGGCACGGCGATCTTCC
GCCTGGACGAGCACACCAAGCGCCTCTTCAACTCGGCGCATATCCTCGGCATGAAACTGCCTTTCGACGCGGCGACGCTT
TCGGAAGCGCAGCGCATGGTCGTGCGCGAGAACAAGCTCGAGTCGGGCTACCTGCGGCCGATGGCGTTCTTCGGCTCGGA
GGCGATGGGCATTTCGGCGAAGAATCTTTCGGTGCACGTGATCGTCGCCGCCTGGCCCTGGGGCACCTACCTCGGCAAGG
AGGCTCTCGAAAACGGAATCCGCGTGAAGACGAGCTCGTTCACGCGTCACCACTCCAACGTGACGATGTGCAAGGCCAAG
GCCAACGGCAACTACATGAACTCGATCCTCGCGCACAAGGAAGCCGAGATGGACGGCTACGACGAGGCGCTGCTGCTCGA
CGTCGACGGCTTCGTCTCCGAAGGCTCCGGCGAGAACATCTTCATCGTGCGCGACGGCAAGCTCTATACGCCCGACCCGA
GCTCCGCGCTCGTCGGCCTCACGCGTGACACGATCATCCAGCTCGCCGAGGAAATCGGCCTCACGGTGATCGAAAAGCGC
ATCACGCGCGACGAGGTCTATATCGCGGACGAGGCGTTCTTCACCGGCACGGCTGCCGAAGTCACGCCGATCCGCGAGCT
GGACAACCGCACGATCGGTTCCGGTAGCCGGGGCCCGGTCACGGAACGCCTGCAATCGATGTACTTCGATTGCGTGCACG
GCCGCGCAGCCAATCACCTCGACTGGCTCACGCCGGTAGCGTAG

Upstream 100 bases:

>100_bases
AGCGCTCCAGACCGCGAAAACATCGCGCAGGCGCGCGCTTCGCGCCCCGGAGATTCCGGTAAAATTCGTGCTTTCCATTC
GTTCACAGAGACTTCTCACC

Downstream 100 bases:

>100_bases
GGAGGCCTCATGAGCGAGCCGCGTGACGACAACCTCGAACGCCCTGATGGCAAAGCGAACAACACCCAGCGCGTGATCGA
AGTCAGCGAAAGCGATCTGC

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT [H]

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MSMADRDGVIWYDGKLVPWRDATTHVLTHTLHYGMGVFEGVRAYKAEQGTAIFRLDEHTKRLFNSAHILGMKLPFDAATL
SEAQRMVVRENKLESGYLRPMAFFGSEAMGISAKNLSVHVIVAAWPWGTYLGKEALENGIRVKTSSFTRHHSNVTMCKAK
ANGNYMNSILAHKEAEMDGYDEALLLDVDGFVSEGSGENIFIVRDGKLYTPDPSSALVGLTRDTIIQLAEEIGLTVIEKR
ITRDEVYIADEAFFTGTAAEVTPIRELDNRTIGSGSRGPVTERLQSMYFDCVHGRAANHLDWLTPVA

Sequences:

>Translated_307_residues
MSMADRDGVIWYDGKLVPWRDATTHVLTHTLHYGMGVFEGVRAYKAEQGTAIFRLDEHTKRLFNSAHILGMKLPFDAATL
SEAQRMVVRENKLESGYLRPMAFFGSEAMGISAKNLSVHVIVAAWPWGTYLGKEALENGIRVKTSSFTRHHSNVTMCKAK
ANGNYMNSILAHKEAEMDGYDEALLLDVDGFVSEGSGENIFIVRDGKLYTPDPSSALVGLTRDTIIQLAEEIGLTVIEKR
ITRDEVYIADEAFFTGTAAEVTPIRELDNRTIGSGSRGPVTERLQSMYFDCVHGRAANHLDWLTPVA
>Mature_306_residues
SMADRDGVIWYDGKLVPWRDATTHVLTHTLHYGMGVFEGVRAYKAEQGTAIFRLDEHTKRLFNSAHILGMKLPFDAATLS
EAQRMVVRENKLESGYLRPMAFFGSEAMGISAKNLSVHVIVAAWPWGTYLGKEALENGIRVKTSSFTRHHSNVTMCKAKA
NGNYMNSILAHKEAEMDGYDEALLLDVDGFVSEGSGENIFIVRDGKLYTPDPSSALVGLTRDTIIQLAEEIGLTVIEKRI
TRDEVYIADEAFFTGTAAEVTPIRELDNRTIGSGSRGPVTERLQSMYFDCVHGRAANHLDWLTPVA

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI50658084, Length=334, Percent_Identity=25.748502994012, Blast_Score=85, Evalue=8e-17,
Organism=Escherichia coli, GI48994963, Length=299, Percent_Identity=47.1571906354515, Blast_Score=288, Evalue=2e-79,
Organism=Caenorhabditis elegans, GI17565728, Length=281, Percent_Identity=28.1138790035587, Blast_Score=83, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6322608, Length=283, Percent_Identity=25.7950530035336, Blast_Score=78, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6322002, Length=333, Percent_Identity=24.024024024024, Blast_Score=73, Evalue=6e-14,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005785 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 34076; Mature: 33945

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMADRDGVIWYDGKLVPWRDATTHVLTHTLHYGMGVFEGVRAYKAEQGTAIFRLDEHTK
CCCCCCCCEEEECCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECHHHH
RLFNSAHILGMKLPFDAATLSEAQRMVVRENKLESGYLRPMAFFGSEAMGISAKNLSVHV
HHHCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCEEECCEEEEE
IVAAWPWGTYLGKEALENGIRVKTSSFTRHHSNVTMCKAKANGNYMNSILAHKEAEMDGY
EEEECCCHHHHHHHHHHCCCEEEECCCCEECCCEEEEEEECCCCHHHHHHHHHHCCCCCC
DEALLLDVDGFVSEGSGENIFIVRDGKLYTPDPSSALVGLTRDTIIQLAEEIGLTVIEKR
CCEEEEEECCCCCCCCCCEEEEEECCEEECCCCCCCEEECHHHHHHHHHHHHCHHHHHHH
ITRDEVYIADEAFFTGTAAEVTPIRELDNRTIGSGSRGPVTERLQSMYFDCVHGRAANHL
HCCCEEEEECCCEECCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCC
DWLTPVA
CCCCCCC
>Mature Secondary Structure 
SMADRDGVIWYDGKLVPWRDATTHVLTHTLHYGMGVFEGVRAYKAEQGTAIFRLDEHTK
CCCCCCCEEEECCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECHHHH
RLFNSAHILGMKLPFDAATLSEAQRMVVRENKLESGYLRPMAFFGSEAMGISAKNLSVHV
HHHCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCEEECCEEEEE
IVAAWPWGTYLGKEALENGIRVKTSSFTRHHSNVTMCKAKANGNYMNSILAHKEAEMDGY
EEEECCCHHHHHHHHHHCCCEEEECCCCEECCCEEEEEEECCCCHHHHHHHHHHCCCCCC
DEALLLDVDGFVSEGSGENIFIVRDGKLYTPDPSSALVGLTRDTIIQLAEEIGLTVIEKR
CCEEEEEECCCCCCCCCCEEEEEECCEEECCCCCCCEEECHHHHHHHHHHHHCHHHHHHH
ITRDEVYIADEAFFTGTAAEVTPIRELDNRTIGSGSRGPVTERLQSMYFDCVHGRAANHL
HCCCEEEEECCCEECCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCC
DWLTPVA
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]