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The map label for this gene is aspA [H]

Identifier: 74318137

GI number: 74318137

Start: 2206976

End: 2208376

Strand: Direct

Name: aspA [H]

Synonym: Tbd_2119

Alternate gene names: 74318137

Gene position: 2206976-2208376 (Clockwise)

Preceding gene: 74318132

Following gene: 74318138

Centisome position: 75.85

GC content: 66.95

Gene sequence:

>1401_bases
ATGAATGAATTCCGCACCGAGAAGGACTCGCTCGGCGAAGTCCGGGTTCCCCGAGACGCGTGGTACGGCGCCCAGACCGC
ACGGGCCGTGAGCAATTTTCCCATTTCGGGCCGCGCGCCGGATAAGGATTTCGTACTAGCGCACGTGCGTATAAAGCTCG
CAGCGGCACGCGCGAATTGCCAGCCAGGGTGGCTGCCGGAACTCAAGCGCGACGCAATCGTCGCGGCCTGCGAGCAGATC
CTCGCGGGCGCGCACCTCGACCAGTTCGTCGTTGACCGCTTCCAGGCGGGTGCGGGCACGAGCCACAACATGAACAGCAA
CGAGGTCATCGCGAACCTTGCCAACGTCGCGCTCGGCGGCGAAAAGGGCCGCTACTCGCCGGTCAACCCGAACGACGACG
TCAACATGGGGCAGTCGACCAACGACACGATCCCGACCGCGATACGTCTCGCAGTGTTGGCGAAGCTGCCCCGCCTGGTC
GCCGCCGTGCGCGGCATGGCCGGTGAATTTTCGCGGCTGGCCGAGCGCGAAAAGGACACGGTGAAATCGGGCCGGACGCA
TTTGCAGGATGCCGTGCCGACGACGCTCGGCCAGGAATTCGACGGCTACGCGTGGACGCTGGCGCGTTGTGCGGCGGCGC
TCGAAGCCGTGCGCCCGGCCCTGTGCGAAATCGGCCTCGGCGGCTCGGCGGCCGGGACGGGACTGAACACCTCGCCCGAT
TATCCGGACCGCGTCGCGGCGGAACTCGCCGCGCTGACCGGCGAATCGATTCGCGCCGGCCAACTCGTCGCGCAGATGCA
GTCGATGAACGACCTTGCGCGGCTGTCCGGCGAAATCCGCAATCTCGCGCTCGAACTCACGCGCATTTCCAACGACCTGC
GCCTGCTTGCCTCGGGGCCGCGTACCGGCCTCGGCGAAATCCAGCTGCCGCCGGTGCAGCCCGGCTCGTCGATCATGCCG
GGCAAGGTCAACCCGGTCATGTTCGAAATGCTGAACCAGGTCTGCTTCCAGGTGCTCGGGCAGGATGCGGCGGTCTCCTA
CATGGTGCAGGCAGGCCAGCTCGAGCTCAACGTGATGATGCCGGCGCTCGGTTCGGCGCTTTTCGACGCCATGGACTGGC
TGACCAACGCAATCGACGCCGCCACCGAGAAGAACCTCAAGGGCATCGTCGCCAACCGGGAGCGCTGTGCGTTTTTCATC
CACCAGAGCGTCGCGCTCGCCACGCTCCTGAACACCCGGATCGGCTACATGGAGGCCGCCGAGGTCGCCAAGGAATCCGA
AAAAACCGGCCGCCCGGTCCGCGACATCGTCGCGCAGCGCGCGCTGATGGATGCCGCCGAGTTCGACGCGCTCGTGCTCG
CCGCGGCGCATGGCGTGGGTAGCGGGGGCGGGGGCGGCTGA

Upstream 100 bases:

>100_bases
GCCGCCTGCGCGACACTCGCCGCCAGTGCGAGCAGCAGTGCGCCAAACCACATGGGATAAAATCGCGTTCTTTGCAACGT
CTGCATTACTTCCCCCGATC

Downstream 100 bases:

>100_bases
GCGCCGACGAGAGACCGCGACATGGTGATCCAGTGGTTCCCGGGGCACATGACGACCGCCCGCAAGAAGGCGGCCGAAAC
GATGGCCCAGATCGACGTCG

Product: aspartate ammonia-lyase

Products: NA

Alternate protein names: Aspartase [H]

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MNEFRTEKDSLGEVRVPRDAWYGAQTARAVSNFPISGRAPDKDFVLAHVRIKLAAARANCQPGWLPELKRDAIVAACEQI
LAGAHLDQFVVDRFQAGAGTSHNMNSNEVIANLANVALGGEKGRYSPVNPNDDVNMGQSTNDTIPTAIRLAVLAKLPRLV
AAVRGMAGEFSRLAEREKDTVKSGRTHLQDAVPTTLGQEFDGYAWTLARCAAALEAVRPALCEIGLGGSAAGTGLNTSPD
YPDRVAAELAALTGESIRAGQLVAQMQSMNDLARLSGEIRNLALELTRISNDLRLLASGPRTGLGEIQLPPVQPGSSIMP
GKVNPVMFEMLNQVCFQVLGQDAAVSYMVQAGQLELNVMMPALGSALFDAMDWLTNAIDAATEKNLKGIVANRERCAFFI
HQSVALATLLNTRIGYMEAAEVAKESEKTGRPVRDIVAQRALMDAAEFDALVLAAAHGVGSGGGGG

Sequences:

>Translated_466_residues
MNEFRTEKDSLGEVRVPRDAWYGAQTARAVSNFPISGRAPDKDFVLAHVRIKLAAARANCQPGWLPELKRDAIVAACEQI
LAGAHLDQFVVDRFQAGAGTSHNMNSNEVIANLANVALGGEKGRYSPVNPNDDVNMGQSTNDTIPTAIRLAVLAKLPRLV
AAVRGMAGEFSRLAEREKDTVKSGRTHLQDAVPTTLGQEFDGYAWTLARCAAALEAVRPALCEIGLGGSAAGTGLNTSPD
YPDRVAAELAALTGESIRAGQLVAQMQSMNDLARLSGEIRNLALELTRISNDLRLLASGPRTGLGEIQLPPVQPGSSIMP
GKVNPVMFEMLNQVCFQVLGQDAAVSYMVQAGQLELNVMMPALGSALFDAMDWLTNAIDAATEKNLKGIVANRERCAFFI
HQSVALATLLNTRIGYMEAAEVAKESEKTGRPVRDIVAQRALMDAAEFDALVLAAAHGVGSGGGGG
>Mature_466_residues
MNEFRTEKDSLGEVRVPRDAWYGAQTARAVSNFPISGRAPDKDFVLAHVRIKLAAARANCQPGWLPELKRDAIVAACEQI
LAGAHLDQFVVDRFQAGAGTSHNMNSNEVIANLANVALGGEKGRYSPVNPNDDVNMGQSTNDTIPTAIRLAVLAKLPRLV
AAVRGMAGEFSRLAEREKDTVKSGRTHLQDAVPTTLGQEFDGYAWTLARCAAALEAVRPALCEIGLGGSAAGTGLNTSPD
YPDRVAAELAALTGESIRAGQLVAQMQSMNDLARLSGEIRNLALELTRISNDLRLLASGPRTGLGEIQLPPVQPGSSIMP
GKVNPVMFEMLNQVCFQVLGQDAAVSYMVQAGQLELNVMMPALGSALFDAMDWLTNAIDAATEKNLKGIVANRERCAFFI
HQSVALATLLNTRIGYMEAAEVAKESEKTGRPVRDIVAQRALMDAAEFDALVLAAAHGVGSGGGGG

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=463, Percent_Identity=40.8207343412527, Blast_Score=304, Evalue=9e-83,
Organism=Escherichia coli, GI87082375, Length=454, Percent_Identity=44.4933920704846, Blast_Score=363, Evalue=1e-101,
Organism=Escherichia coli, GI1787896, Length=454, Percent_Identity=41.6299559471366, Blast_Score=336, Evalue=2e-93,
Organism=Escherichia coli, GI1790398, Length=314, Percent_Identity=28.0254777070064, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17553882, Length=452, Percent_Identity=40.2654867256637, Blast_Score=315, Evalue=4e-86,
Organism=Caenorhabditis elegans, GI32565146, Length=315, Percent_Identity=39.0476190476191, Blast_Score=219, Evalue=3e-57,
Organism=Saccharomyces cerevisiae, GI6324993, Length=461, Percent_Identity=37.7440347071584, Blast_Score=292, Evalue=7e-80,
Organism=Drosophila melanogaster, GI24640179, Length=456, Percent_Identity=41.0087719298246, Blast_Score=318, Evalue=6e-87,
Organism=Drosophila melanogaster, GI24640177, Length=456, Percent_Identity=41.0087719298246, Blast_Score=317, Evalue=8e-87,
Organism=Drosophila melanogaster, GI78710009, Length=438, Percent_Identity=40.4109589041096, Blast_Score=308, Evalue=6e-84,
Organism=Drosophila melanogaster, GI24662684, Length=457, Percent_Identity=40.0437636761488, Blast_Score=306, Evalue=2e-83,
Organism=Drosophila melanogaster, GI24583245, Length=450, Percent_Identity=36.2222222222222, Blast_Score=280, Evalue=2e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004708
- InterPro:   IPR003031
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.3.1.1 [H]

Molecular weight: Translated: 49484; Mature: 49484

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEFRTEKDSLGEVRVPRDAWYGAQTARAVSNFPISGRAPDKDFVLAHVRIKLAAARANC
CCCCCCCHHCCCCEECCHHHHCCHHHHHHHHCCCCCCCCCCCCEEEEEHHEEEEHHHCCC
QPGWLPELKRDAIVAACEQILAGAHLDQFVVDRFQAGAGTSHNMNSNEVIANLANVALGG
CCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCC
EKGRYSPVNPNDDVNMGQSTNDTIPTAIRLAVLAKLPRLVAAVRGMAGEFSRLAEREKDT
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VKSGRTHLQDAVPTTLGQEFDGYAWTLARCAAALEAVRPALCEIGLGGSAAGTGLNTSPD
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
YPDRVAAELAALTGESIRAGQLVAQMQSMNDLARLSGEIRNLALELTRISNDLRLLASGP
CHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEECCC
RTGLGEIQLPPVQPGSSIMPGKVNPVMFEMLNQVCFQVLGQDAAVSYMVQAGQLELNVMM
CCCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEEEHH
PALGSALFDAMDWLTNAIDAATEKNLKGIVANRERCAFFIHQSVALATLLNTRIGYMEAA
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHCHHHHH
EVAKESEKTGRPVRDIVAQRALMDAAEFDALVLAAAHGVGSGGGGG
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MNEFRTEKDSLGEVRVPRDAWYGAQTARAVSNFPISGRAPDKDFVLAHVRIKLAAARANC
CCCCCCCHHCCCCEECCHHHHCCHHHHHHHHCCCCCCCCCCCCEEEEEHHEEEEHHHCCC
QPGWLPELKRDAIVAACEQILAGAHLDQFVVDRFQAGAGTSHNMNSNEVIANLANVALGG
CCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCC
EKGRYSPVNPNDDVNMGQSTNDTIPTAIRLAVLAKLPRLVAAVRGMAGEFSRLAEREKDT
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VKSGRTHLQDAVPTTLGQEFDGYAWTLARCAAALEAVRPALCEIGLGGSAAGTGLNTSPD
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
YPDRVAAELAALTGESIRAGQLVAQMQSMNDLARLSGEIRNLALELTRISNDLRLLASGP
CHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEECCC
RTGLGEIQLPPVQPGSSIMPGKVNPVMFEMLNQVCFQVLGQDAAVSYMVQAGQLELNVMM
CCCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEEEHH
PALGSALFDAMDWLTNAIDAATEKNLKGIVANRERCAFFIHQSVALATLLNTRIGYMEAA
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHCHHHHH
EVAKESEKTGRPVRDIVAQRALMDAAEFDALVLAAAHGVGSGGGGG
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7789816; 12948626 [H]