Click here to switch to the map view.

The map label for this gene is aat

Identifier: 74318061

GI number: 74318061

Start: 2131059

End: 2131745

Strand: Reverse

Name: aat

Synonym: Tbd_2043

Alternate gene names: 74318061

Gene position: 2131745-2131059 (Counterclockwise)

Preceding gene: 74318074

Following gene: 74318060

Centisome position: 73.26

GC content: 68.56

Gene sequence:

>687_bases
ATGAAGGAGCCCTTCACGAACGCGTCGTATTTCCCGCCCGTGGAGACCGCGCTGGTCGACCCGAACGGGCTGCTGGCGAT
CGGCGGAGACCTCTCGCAAGAGCGACTGCTCGACGCCTATCGCCACGGCATCTTTCCCTGGTTCAACCCGCGCGAGCCGA
TCCAGTGGTGGAGCCCCGATCCGCGCATGGTGCTGCCGCCCGCCGAGATCCGCGTCACCCGCTCGCTCGCCAAGCGGCTG
CGCAACGCCGGTTTCGAACTGCGTGTCGACAGCGCGTTCATCGAGGTGATGCGCGCCTGCGGGGCGCCGCGCGAAGGCGC
GTGGGGGACCTGGATCTCGGCGCCGATGATCGCCGCCTACGGTCGCCTGTTCGACGCGGGCTACGCGCATTCGATCGAGA
CCTGGCGCGACGGCCGGCTCGTCGGCGGGCTCTACGGCGTCGCGATCGGCCGCATGTTCTACGGCGAGTCGATGTTCAGC
CGCGAGCCCGATGCGTCGAAAGTCGCGCTGGTCCGGCTCGCGAGGCAGCTCGAGCGCTGGGGATTTGGCCTGATCGACTG
CCAGATGGAAACGCCGCACCTCGCCAGCATGGGCGCGCGTCCGATTCCGCGCGCCGACTTCACAGCGCGGCTGGCCGAAT
TGGTAAACTTGCCGCATCTGCCCGGGCCCTGGACGTTTGACAGCTGA

Upstream 100 bases:

>100_bases
TTCCGCCAGATAGCAGCATTGAAGATGCGCATATCATAATTAACTTATCCTGACGTGGCAAGCGGATTTCGAGCGAACAC
GATTCCGCTACCATGACGGC

Downstream 100 bases:

>100_bases
TGCACCCGACCGAACCGCCTTTTCAGCGCATCCAGTTCTACCTGACCGCGCAATACGACTGCAGTTACCTCCCGGGCCGG
CTCGCCCGTTCGCAGGTCGC

Product: leucyl/phenylalanyl-tRNA--protein transferase

Products: NA

Alternate protein names: L/F-transferase; Leucyltransferase; Phenyalanyltransferase

Number of amino acids: Translated: 228; Mature: 228

Protein sequence:

>228_residues
MKEPFTNASYFPPVETALVDPNGLLAIGGDLSQERLLDAYRHGIFPWFNPREPIQWWSPDPRMVLPPAEIRVTRSLAKRL
RNAGFELRVDSAFIEVMRACGAPREGAWGTWISAPMIAAYGRLFDAGYAHSIETWRDGRLVGGLYGVAIGRMFYGESMFS
REPDASKVALVRLARQLERWGFGLIDCQMETPHLASMGARPIPRADFTARLAELVNLPHLPGPWTFDS

Sequences:

>Translated_228_residues
MKEPFTNASYFPPVETALVDPNGLLAIGGDLSQERLLDAYRHGIFPWFNPREPIQWWSPDPRMVLPPAEIRVTRSLAKRL
RNAGFELRVDSAFIEVMRACGAPREGAWGTWISAPMIAAYGRLFDAGYAHSIETWRDGRLVGGLYGVAIGRMFYGESMFS
REPDASKVALVRLARQLERWGFGLIDCQMETPHLASMGARPIPRADFTARLAELVNLPHLPGPWTFDS
>Mature_228_residues
MKEPFTNASYFPPVETALVDPNGLLAIGGDLSQERLLDAYRHGIFPWFNPREPIQWWSPDPRMVLPPAEIRVTRSLAKRL
RNAGFELRVDSAFIEVMRACGAPREGAWGTWISAPMIAAYGRLFDAGYAHSIETWRDGRLVGGLYGVAIGRMFYGESMFS
REPDASKVALVRLARQLERWGFGLIDCQMETPHLASMGARPIPRADFTARLAELVNLPHLPGPWTFDS

Specific function: Functions in the N-end rule pathway of protein degradation where it conjugates Leu, Phe and, less efficiently, Met from aminoacyl-tRNAs to the N-termini of proteins containing an N-terminal arginine or lysine

COG id: COG2360

COG function: function code O; Leu/Phe-tRNA-protein transferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the L/F-transferase family

Homologues:

Organism=Escherichia coli, GI1787111, Length=204, Percent_Identity=50, Blast_Score=201, Evalue=3e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LFTR_THIDA (Q3SH93)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_315801.1
- HSSP:   P0A8P1
- ProteinModelPortal:   Q3SH93
- SMR:   Q3SH93
- STRING:   Q3SH93
- GeneID:   3672214
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_2043
- NMPDR:   fig|292415.3.peg.2183
- eggNOG:   COG2360
- HOGENOM:   HBG485363
- OMA:   GEPILWW
- PhylomeDB:   Q3SH93
- ProtClustDB:   PRK00301
- BioCyc:   TDEN292415:TBD_2043-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00688
- InterPro:   IPR016181
- InterPro:   IPR004616
- TIGRFAMs:   TIGR00667

Pfam domain/function: PF03588 Leu_Phe_trans; SSF55729 Acyl_CoA_acyltransferase

EC number: =2.3.2.6

Molecular weight: Translated: 25574; Mature: 25574

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEPFTNASYFPPVETALVDPNGLLAIGGDLSQERLLDAYRHGIFPWFNPREPIQWWSPD
CCCCCCCCCCCCCCHHEEECCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCC
PRMVLPPAEIRVTRSLAKRLRNAGFELRVDSAFIEVMRACGAPREGAWGTWISAPMIAAY
CCEEECCHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
GRLFDAGYAHSIETWRDGRLVGGLYGVAIGRMFYGESMFSREPDASKVALVRLARQLERW
HHHHHCCCHHCHHHCCCCCCHHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHH
GFGLIDCQMETPHLASMGARPIPRADFTARLAELVNLPHLPGPWTFDS
CCCEEEEECCCCHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MKEPFTNASYFPPVETALVDPNGLLAIGGDLSQERLLDAYRHGIFPWFNPREPIQWWSPD
CCCCCCCCCCCCCCHHEEECCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCC
PRMVLPPAEIRVTRSLAKRLRNAGFELRVDSAFIEVMRACGAPREGAWGTWISAPMIAAY
CCEEECCHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
GRLFDAGYAHSIETWRDGRLVGGLYGVAIGRMFYGESMFSREPDASKVALVRLARQLERW
HHHHHCCCHHCHHHCCCCCCHHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHH
GFGLIDCQMETPHLASMGARPIPRADFTARLAELVNLPHLPGPWTFDS
CCCEEEEECCCCHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA