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The map label for this gene is nrdB [H]

Identifier: 74317993

GI number: 74317993

Start: 2064524

End: 2065654

Strand: Reverse

Name: nrdB [H]

Synonym: Tbd_1975

Alternate gene names: 74317993

Gene position: 2065654-2064524 (Counterclockwise)

Preceding gene: 74317994

Following gene: 74317992

Centisome position: 70.99

GC content: 62.69

Gene sequence:

>1131_bases
ATGCTGAACTTTGAAGACGACTTCACGCCCGCCCCGCAACTCGTGCCGCGCGTGCCCGAGCCCGCGTATGTGCCGGCCGC
CGCCGCTGCGCCTGCCGCCCAGCCGGCCGCGACGACTCGCGTGCGCGCCTCCGATAAGCGTGTCATCAACGGCGCCACCG
ACGTGAACCAGCTCGTTCCCTTCAAGTACAAGTGGGCGTGGGAGAAATACCTCGCCGGCTGCGCCAACCACTGGATGCCG
CAGGAAGTGAACATGAGCCGCGACATCGCGCAGTGGAAGGACCCGACCGCGCTGACCGAGGACGAGCGCCGCATCGTCAA
GCGCAACCTCGGCTTCTTCGTGACGGCCGATTCGCTCGCCGCCAACAACATCGTGCTCGGCACCTACCGCCACATCACAG
CGCCCGAGTGCCGCCAGTACCTGCTGCGCCAGGCCTTCGAAGAGGCGATCCACACCCACGCCTACCAGTACATCGTCGAA
AGCCTGGGCCTCGACGAGGCGGAAATCTTCAACGCCTACCACGAGATCGAGAGCATCCGCGACAAGGACGACTTCCTCAT
CCCCTTCATCGATACGCTCACCAACCCCGAATTCAAGACCGGCACGCCGGAGAACGACCAGAAACTGCTGAAGTCGCTGA
TCGTCTTCGCCTGCATCATGGAAGGCATCTTCTTCTATGTCGGCTTCGTGCAGATCCTCGCGCTCGGCCGCCAGAACAAG
ATGACCGGCGCCGCCGAGCAGTACCAGTACATCCTGCGCGACGAGTCCATGCACTGCAACTTCGGCATCGACCTCATCAA
CACCATCAAGCAGGAAAACCCGCACCTCTGGACCAGCGAGTTCAAGGCCGAGATCCGCACCCTGATCCAGAAGGGCGTCG
AGCTCGAATTCCGCTACGCCGAAGACACCATGCCGCGCGGCGTGCTGGGCCTCAACTCCGCTCAGTTCAAGGAATACCTG
CGCTTCATCTCCAACCGCCGCTGCCAGCAGATCGGGCTGGACGAGATGTTCCCCGGCGTCAGCAACCCCTTCCCCTGGAT
GGCCGAGATGATCGACCTGAAGAAGGAGAAGAACTTCTTCGAGACGCGCGTCACCGAGTATCAGACGGGCGGGGCGTTGA
GCTGGGATTGA

Upstream 100 bases:

>100_bases
GTGGGGCGCAGGGGATCGAAGCCCTTCCGGCCGACCGTAAAGATGAATATGTAGTGTGGGCCCCGGCCCACTTTGGGAAA
CCCAAAGGAGAGAAAAAACG

Downstream 100 bases:

>100_bases
CATGGCGAAGCTCGCGAAGCTGATCGAGCAGATCCTGCTCGGCCGCAGCGATGCCAATATCGGCTTCGACGAGCTGTGCC
AGGTCCTGACCAGCCTGGGT

Product: ribonucleotide-diphosphate reductase subunit beta

Products: NA

Alternate protein names: Ribonucleotide reductase small subunit [H]

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MLNFEDDFTPAPQLVPRVPEPAYVPAAAAAPAAQPAATTRVRASDKRVINGATDVNQLVPFKYKWAWEKYLAGCANHWMP
QEVNMSRDIAQWKDPTALTEDERRIVKRNLGFFVTADSLAANNIVLGTYRHITAPECRQYLLRQAFEEAIHTHAYQYIVE
SLGLDEAEIFNAYHEIESIRDKDDFLIPFIDTLTNPEFKTGTPENDQKLLKSLIVFACIMEGIFFYVGFVQILALGRQNK
MTGAAEQYQYILRDESMHCNFGIDLINTIKQENPHLWTSEFKAEIRTLIQKGVELEFRYAEDTMPRGVLGLNSAQFKEYL
RFISNRRCQQIGLDEMFPGVSNPFPWMAEMIDLKKEKNFFETRVTEYQTGGALSWD

Sequences:

>Translated_376_residues
MLNFEDDFTPAPQLVPRVPEPAYVPAAAAAPAAQPAATTRVRASDKRVINGATDVNQLVPFKYKWAWEKYLAGCANHWMP
QEVNMSRDIAQWKDPTALTEDERRIVKRNLGFFVTADSLAANNIVLGTYRHITAPECRQYLLRQAFEEAIHTHAYQYIVE
SLGLDEAEIFNAYHEIESIRDKDDFLIPFIDTLTNPEFKTGTPENDQKLLKSLIVFACIMEGIFFYVGFVQILALGRQNK
MTGAAEQYQYILRDESMHCNFGIDLINTIKQENPHLWTSEFKAEIRTLIQKGVELEFRYAEDTMPRGVLGLNSAQFKEYL
RFISNRRCQQIGLDEMFPGVSNPFPWMAEMIDLKKEKNFFETRVTEYQTGGALSWD
>Mature_376_residues
MLNFEDDFTPAPQLVPRVPEPAYVPAAAAAPAAQPAATTRVRASDKRVINGATDVNQLVPFKYKWAWEKYLAGCANHWMP
QEVNMSRDIAQWKDPTALTEDERRIVKRNLGFFVTADSLAANNIVLGTYRHITAPECRQYLLRQAFEEAIHTHAYQYIVE
SLGLDEAEIFNAYHEIESIRDKDDFLIPFIDTLTNPEFKTGTPENDQKLLKSLIVFACIMEGIFFYVGFVQILALGRQNK
MTGAAEQYQYILRDESMHCNFGIDLINTIKQENPHLWTSEFKAEIRTLIQKGVELEFRYAEDTMPRGVLGLNSAQFKEYL
RFISNRRCQQIGLDEMFPGVSNPFPWMAEMIDLKKEKNFFETRVTEYQTGGALSWD

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0208

COG function: function code F; Ribonucleotide reductase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase small chain family [H]

Homologues:

Organism=Homo sapiens, GI4557845, Length=358, Percent_Identity=27.0949720670391, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI260064013, Length=358, Percent_Identity=27.0949720670391, Blast_Score=140, Evalue=1e-33,
Organism=Homo sapiens, GI289177074, Length=367, Percent_Identity=24.2506811989101, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI42544136, Length=339, Percent_Identity=24.4837758112094, Blast_Score=136, Evalue=4e-32,
Organism=Homo sapiens, GI289177076, Length=296, Percent_Identity=26.0135135135135, Blast_Score=121, Evalue=1e-27,
Organism=Escherichia coli, GI1788567, Length=291, Percent_Identity=26.8041237113402, Blast_Score=100, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17551968, Length=350, Percent_Identity=25.7142857142857, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI193206379, Length=304, Percent_Identity=22.3684210526316, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17567153, Length=224, Percent_Identity=25.4464285714286, Blast_Score=71, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6322434, Length=330, Percent_Identity=29.3939393939394, Blast_Score=150, Evalue=4e-37,
Organism=Saccharomyces cerevisiae, GI6321619, Length=320, Percent_Identity=22.8125, Blast_Score=91, Evalue=4e-19,
Organism=Drosophila melanogaster, GI18079279, Length=320, Percent_Identity=26.875, Blast_Score=137, Evalue=8e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009078
- InterPro:   IPR012348
- InterPro:   IPR000358 [H]

Pfam domain/function: PF00268 Ribonuc_red_sm [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 43178; Mature: 43178

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNFEDDFTPAPQLVPRVPEPAYVPAAAAAPAAQPAATTRVRASDKRVINGATDVNQLVP
CCCCCCCCCCCCHHCCCCCCCCCCCCHHCCCCCCCCHHHHHCCCCCHHCCCCCCHHHHCC
FKYKWAWEKYLAGCANHWMPQEVNMSRDIAQWKDPTALTEDERRIVKRNLGFFVTADSLA
CHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHCCCEEEEECCCC
ANNIVLGTYRHITAPECRQYLLRQAFEEAIHTHAYQYIVESLGLDEAEIFNAYHEIESIR
CCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHC
DKDDFLIPFIDTLTNPEFKTGTPENDQKLLKSLIVFACIMEGIFFYVGFVQILALGRQNK
CCCCEEEHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
MTGAAEQYQYILRDESMHCNFGIDLINTIKQENPHLWTSEFKAEIRTLIQKGVELEFRYA
CCCHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEEEC
EDTMPRGVLGLNSAQFKEYLRFISNRRCQQIGLDEMFPGVSNPFPWMAEMIDLKKEKNFF
CCCCCCCCCCCCHHHHHHHHHHHHCCHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHHHHH
ETRVTEYQTGGALSWD
HHHHHHHCCCCCCCCC
>Mature Secondary Structure
MLNFEDDFTPAPQLVPRVPEPAYVPAAAAAPAAQPAATTRVRASDKRVINGATDVNQLVP
CCCCCCCCCCCCHHCCCCCCCCCCCCHHCCCCCCCCHHHHHCCCCCHHCCCCCCHHHHCC
FKYKWAWEKYLAGCANHWMPQEVNMSRDIAQWKDPTALTEDERRIVKRNLGFFVTADSLA
CHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHCCCEEEEECCCC
ANNIVLGTYRHITAPECRQYLLRQAFEEAIHTHAYQYIVESLGLDEAEIFNAYHEIESIR
CCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHC
DKDDFLIPFIDTLTNPEFKTGTPENDQKLLKSLIVFACIMEGIFFYVGFVQILALGRQNK
CCCCEEEHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
MTGAAEQYQYILRDESMHCNFGIDLINTIKQENPHLWTSEFKAEIRTLIQKGVELEFRYA
CCCHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEEEC
EDTMPRGVLGLNSAQFKEYLRFISNRRCQQIGLDEMFPGVSNPFPWMAEMIDLKKEKNFF
CCCCCCCCCCCCHHHHHHHHHHHHCCHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHHHHH
ETRVTEYQTGGALSWD
HHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]