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The map label for this gene is iaaA [H]

Identifier: 74317949

GI number: 74317949

Start: 2026696

End: 2027721

Strand: Reverse

Name: iaaA [H]

Synonym: Tbd_1931

Alternate gene names: 74317949

Gene position: 2027721-2026696 (Counterclockwise)

Preceding gene: 74317950

Following gene: 74317947

Centisome position: 69.69

GC content: 73.1

Gene sequence:

>1026_bases
ATGCTTGAACCCCGTCTCCCGACCGGCCGCGCCGCCCCGCGCGGCGTCTTCGCGATGCCCATAGACGGCGGCGAAGGCGG
CGCCGCGGGCGAAATCGACGTGCGGCCCGCGGAGCTCGGTCAGTCGGGCGGCTTCCAGCCGCTGGTCGTGGTGCACGGCG
GCGCGGGCGCGCCGATGACGTGGCTGGACGGCTGCACCCACGCGGCGGAGGCCGGTGCGGCGGTTCTGAAGAACGGGCTC
GACGCGTTGAGTGCGGCGATCGCCTCGGTGATCGCGCTCGAAGCCGACGGGCGGTTCAACGCCGGGCTCGGTGCCTCGCT
CAAGCTCGACGGCGACGCCGTCGAGATGGACGCGGCAGTCATGGACTCGACGGGCCGTCTCGGCGCGGTCGCCGCGCTGC
AGGACGTGCGCCATCCCGTCCTCGCCGCACGCGCGGTCGCCGACACCGCACACTGGCTGCTGGTCGGCGACGGCGCGCGC
GAATTCGCCGCGCGCTGCAGCCTGACCGGCAACCATGAGCCGAGCGCCCGGGCGCGTGAGCGCTACGCCGAGGTGGTGTC
GGAACTGGCCCGCTTCTGGTGCGAGTCGCCCGACGCCGACCTGCCCTTGCGCCGGTTCTGGAACTACGCGTTGCCGTGGG
ACGAGGCCTTGAAGAAATGGGGGTGCGGCACCGTCGGCGCAGTGGTTCGCGATGCGACCGGCCAGCTCGCGGTCGCGACG
TCGACCGGTGGTGCGGTGCCGGCCTTGCGCGGCCGGGTCGGCGATACCCCGATCATCGGCAGCGGGTTTTATGCGGGCCC
ACGCGCCGCGGTCGCCGTGACCGGGGCCGGCGAGCAGATCGTGCGCACCCTGCTCGCGAATACCGTGTATCGCTGGATCG
AAACCGGCCTGCCGCCGGCCGATGCCCTGAAGCAAGGCCTCGCCCTGTTCCCGCCCGACGTGGACATCGGCATCATCGCG
GTCTCCGAGCGCGAATCCGCCGGCCTCAGCAGCCGCGGCATGCCGTGGTGCCAGGCGGCCGTCTGA

Upstream 100 bases:

>100_bases
ATCCCACAGCGGTTGGCGCTGTCCTCCGATATCGCGGGCGCCGGCAGGCGGAAATACTGATGCCTTGATCGTGTACCCAT
TCCCGCCTCGTGCCCGCCCA

Downstream 100 bases:

>100_bases
GCCCGGCGCGCTTCGATCACCCCCGTTCCCGATTTTCCGCAGTCCTCGGTACGAAAGGCCGCCCGCCTTCGTTCATTCCG
GCCCCGACGCCGACGATCGG

Product: asparaginase

Products: NA

Alternate protein names: Beta-aspartyl-peptidase; Isoaspartyl dipeptidase [H]

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MLEPRLPTGRAAPRGVFAMPIDGGEGGAAGEIDVRPAELGQSGGFQPLVVVHGGAGAPMTWLDGCTHAAEAGAAVLKNGL
DALSAAIASVIALEADGRFNAGLGASLKLDGDAVEMDAAVMDSTGRLGAVAALQDVRHPVLAARAVADTAHWLLVGDGAR
EFAARCSLTGNHEPSARARERYAEVVSELARFWCESPDADLPLRRFWNYALPWDEALKKWGCGTVGAVVRDATGQLAVAT
STGGAVPALRGRVGDTPIIGSGFYAGPRAAVAVTGAGEQIVRTLLANTVYRWIETGLPPADALKQGLALFPPDVDIGIIA
VSERESAGLSSRGMPWCQAAV

Sequences:

>Translated_341_residues
MLEPRLPTGRAAPRGVFAMPIDGGEGGAAGEIDVRPAELGQSGGFQPLVVVHGGAGAPMTWLDGCTHAAEAGAAVLKNGL
DALSAAIASVIALEADGRFNAGLGASLKLDGDAVEMDAAVMDSTGRLGAVAALQDVRHPVLAARAVADTAHWLLVGDGAR
EFAARCSLTGNHEPSARARERYAEVVSELARFWCESPDADLPLRRFWNYALPWDEALKKWGCGTVGAVVRDATGQLAVAT
STGGAVPALRGRVGDTPIIGSGFYAGPRAAVAVTGAGEQIVRTLLANTVYRWIETGLPPADALKQGLALFPPDVDIGIIA
VSERESAGLSSRGMPWCQAAV
>Mature_341_residues
MLEPRLPTGRAAPRGVFAMPIDGGEGGAAGEIDVRPAELGQSGGFQPLVVVHGGAGAPMTWLDGCTHAAEAGAAVLKNGL
DALSAAIASVIALEADGRFNAGLGASLKLDGDAVEMDAAVMDSTGRLGAVAALQDVRHPVLAARAVADTAHWLLVGDGAR
EFAARCSLTGNHEPSARARERYAEVVSELARFWCESPDADLPLRRFWNYALPWDEALKKWGCGTVGAVVRDATGQLAVAT
STGGAVPALRGRVGDTPIIGSGFYAGPRAAVAVTGAGEQIVRTLLANTVYRWIETGLPPADALKQGLALFPPDVDIGIIA
VSERESAGLSSRGMPWCQAAV

Specific function: Degrades proteins damaged by L-isoaspartyl residue formation (also known as beta-Asp residues). Degrades L- isoaspartyl-containing di- and tripeptides. Acts best on iso-Asp- Leu, followed by iso-Asp-Ala, -His and to a lesser extent iso-Asp- Lys, -Phe and

COG id: COG1446

COG function: function code E; Asparaginase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Ntn-hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI145275202, Length=311, Percent_Identity=35.048231511254, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI145275200, Length=311, Percent_Identity=35.048231511254, Blast_Score=126, Evalue=2e-29,
Organism=Escherichia coli, GI1787050, Length=270, Percent_Identity=36.2962962962963, Blast_Score=120, Evalue=9e-29,
Organism=Caenorhabditis elegans, GI17569239, Length=267, Percent_Identity=30.3370786516854, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI18921183, Length=263, Percent_Identity=33.0798479087452, Blast_Score=101, Evalue=7e-22,
Organism=Drosophila melanogaster, GI21357893, Length=275, Percent_Identity=29.8181818181818, Blast_Score=84, Evalue=9e-17,
Organism=Drosophila melanogaster, GI221330102, Length=211, Percent_Identity=30.8056872037915, Blast_Score=78, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24652135, Length=211, Percent_Identity=30.8056872037915, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000246 [H]

Pfam domain/function: PF01112 Asparaginase_2 [H]

EC number: =3.4.19.5 [H]

Molecular weight: Translated: 35104; Mature: 35104

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEPRLPTGRAAPRGVFAMPIDGGEGGAAGEIDVRPAELGQSGGFQPLVVVHGGAGAPMT
CCCCCCCCCCCCCCCEEEEEECCCCCCCCCEEECCHHHHCCCCCCCEEEEEECCCCCCHH
WLDGCTHAAEAGAAVLKNGLDALSAAIASVIALEADGRFNAGLGASLKLDGDAVEMDAAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCEEEECCCCEEHHHHH
MDSTGRLGAVAALQDVRHPVLAARAVADTAHWLLVGDGAREFAARCSLTGNHEPSARARE
HCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHCCCCCCCCCCHHHHH
RYAEVVSELARFWCESPDADLPLRRFWNYALPWDEALKKWGCGTVGAVVRDATGQLAVAT
HHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCHHHHHHHCCCCHHHHHHHCCCCCEEEEE
STGGAVPALRGRVGDTPIIGSGFYAGPRAAVAVTGAGEQIVRTLLANTVYRWIETGLPPA
CCCCCCHHHHCCCCCCCEECCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCHH
DALKQGLALFPPDVDIGIIAVSERESAGLSSRGMPWCQAAV
HHHHCCCEECCCCCCEEEEEECCCCCCCCCCCCCCHHHCCC
>Mature Secondary Structure
MLEPRLPTGRAAPRGVFAMPIDGGEGGAAGEIDVRPAELGQSGGFQPLVVVHGGAGAPMT
CCCCCCCCCCCCCCCEEEEEECCCCCCCCCEEECCHHHHCCCCCCCEEEEEECCCCCCHH
WLDGCTHAAEAGAAVLKNGLDALSAAIASVIALEADGRFNAGLGASLKLDGDAVEMDAAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCEEEECCCCEEHHHHH
MDSTGRLGAVAALQDVRHPVLAARAVADTAHWLLVGDGAREFAARCSLTGNHEPSARARE
HCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHCCCCCCCCCCHHHHH
RYAEVVSELARFWCESPDADLPLRRFWNYALPWDEALKKWGCGTVGAVVRDATGQLAVAT
HHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCHHHHHHHCCCCHHHHHHHCCCCCEEEEE
STGGAVPALRGRVGDTPIIGSGFYAGPRAAVAVTGAGEQIVRTLLANTVYRWIETGLPPA
CCCCCCHHHHCCCCCCCEECCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCHH
DALKQGLALFPPDVDIGIIAVSERESAGLSSRGMPWCQAAV
HHHHCCCEECCCCCCEEEEEECCCCCCCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]