Click here to switch to the map view.

The map label for this gene is pleD [H]

Identifier: 74317945

GI number: 74317945

Start: 2023227

End: 2023988

Strand: Direct

Name: pleD [H]

Synonym: Tbd_1927

Alternate gene names: 74317945

Gene position: 2023227-2023988 (Clockwise)

Preceding gene: 74317944

Following gene: 74317946

Centisome position: 69.53

GC content: 68.11

Gene sequence:

>762_bases
GTGGCCACGCTGAGCTCCGTCTCCCCGATCGATCTGCACGCGCTGCGGCTCGACGACGCGCGGGCGCTGCTCGGCCACGC
CGGCAAGCCCGGGGCTCCGGAGGCCGGCGAGGCCGCGGTCGAATACCTCCAGCGCGTCATCGACGAACTTTGTGAACTCT
CCTTGAAGGATCCGCTGACCGGCCTGGGCAACCGCCGGCAGTTTCGTGCTGCCCTCGAGCGCGCGATCGAGATCGTCGCG
CGCTCGGGGGACTCGGTACTACTGCTGCTGCTCGACATCGACCACTTCAAGCAGATCAACGACGCCCACGGCCATCTGGT
CGGCGATCAGGTGCTGCAGGCCGTTGCTCGCGCGCTCGCCGAGTCGATCCGCCCGATGGACACGGTCGCGCGCTACGGCG
GTGAGGAATTCGCGATGATTCTGCCCAATTGCCTGCCGGCCTGCGGGCACGCCGTCGCCGAACGCATTCGTCACCGCATC
GAGGCGCTGTCGGTCCAGGTTGCGCCGCACACCGCGCTGCGGGTGACCGCGAGCATCGGCGGCGCCTACGCGCCGCAATG
GGTGAGATCGACCGTGGATCTCTGGGTCGAACGCGCCGACCAGCAGTTGTACCGCGCGAAAGCGGACGGGCGCAATCTCG
TGTTTCTCGACCAGCCGCAGGAAAGCGCCGTCAGCGCCGAAGAAAAAGGCCTGCTCTTCAGCCCGATCGCGGTCACCGAG
GCGCAACCGTCCGCGGAAAATTCAGCGGGCAGCCCGGCCTGA

Upstream 100 bases:

>100_bases
TGATCAAAGCCCAGAAACCCCACATACTCCCGGGGCTCCCGGCCGGAGCCTCACCGGCCGCCTGCCCTTTTACAGCCGGT
ACTAGAACAGAAAAAACGCT

Downstream 100 bases:

>100_bases
CAGCCGGGCCACGCGGTTCCGCCAGTCTATCCATGAACGACGTTCTTTCCCCCGTCCAAGCGGCCACCACGCGGCTTCCG
CTCAAGCCGCCCGGCAAGGT

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 253; Mature: 252

Protein sequence:

>253_residues
MATLSSVSPIDLHALRLDDARALLGHAGKPGAPEAGEAAVEYLQRVIDELCELSLKDPLTGLGNRRQFRAALERAIEIVA
RSGDSVLLLLLDIDHFKQINDAHGHLVGDQVLQAVARALAESIRPMDTVARYGGEEFAMILPNCLPACGHAVAERIRHRI
EALSVQVAPHTALRVTASIGGAYAPQWVRSTVDLWVERADQQLYRAKADGRNLVFLDQPQESAVSAEEKGLLFSPIAVTE
AQPSAENSAGSPA

Sequences:

>Translated_253_residues
MATLSSVSPIDLHALRLDDARALLGHAGKPGAPEAGEAAVEYLQRVIDELCELSLKDPLTGLGNRRQFRAALERAIEIVA
RSGDSVLLLLLDIDHFKQINDAHGHLVGDQVLQAVARALAESIRPMDTVARYGGEEFAMILPNCLPACGHAVAERIRHRI
EALSVQVAPHTALRVTASIGGAYAPQWVRSTVDLWVERADQQLYRAKADGRNLVFLDQPQESAVSAEEKGLLFSPIAVTE
AQPSAENSAGSPA
>Mature_252_residues
ATLSSVSPIDLHALRLDDARALLGHAGKPGAPEAGEAAVEYLQRVIDELCELSLKDPLTGLGNRRQFRAALERAIEIVAR
SGDSVLLLLLDIDHFKQINDAHGHLVGDQVLQAVARALAESIRPMDTVARYGGEEFAMILPNCLPACGHAVAERIRHRIE
ALSVQVAPHTALRVTASIGGAYAPQWVRSTVDLWVERADQQLYRAKADGRNLVFLDQPQESAVSAEEKGLLFSPIAVTEA
QPSAENSAGSPA

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI87081881, Length=173, Percent_Identity=40.4624277456647, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1787262, Length=161, Percent_Identity=38.5093167701863, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI145693134, Length=161, Percent_Identity=37.2670807453416, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1786584, Length=160, Percent_Identity=34.375, Blast_Score=100, Evalue=7e-23,
Organism=Escherichia coli, GI1787802, Length=133, Percent_Identity=43.609022556391, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI87082007, Length=125, Percent_Identity=40, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1787816, Length=168, Percent_Identity=36.9047619047619, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI87081974, Length=165, Percent_Identity=31.5151515151515, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI1788085, Length=163, Percent_Identity=33.1288343558282, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI1788381, Length=188, Percent_Identity=28.1914893617021, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1787541, Length=177, Percent_Identity=30.5084745762712, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1788956, Length=162, Percent_Identity=32.7160493827161, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1787056, Length=126, Percent_Identity=37.3015873015873, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI87081977, Length=124, Percent_Identity=33.0645161290323, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 27201; Mature: 27070

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATLSSVSPIDLHALRLDDARALLGHAGKPGAPEAGEAAVEYLQRVIDELCELSLKDPLT
CCCCCCCCCCCEEEEECCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
GLGNRRQFRAALERAIEIVARSGDSVLLLLLDIDHFKQINDAHGHLVGDQVLQAVARALA
CCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHH
ESIRPMDTVARYGGEEFAMILPNCLPACGHAVAERIRHRIEALSVQVAPHTALRVTASIG
HHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHEEEEHHHC
GAYAPQWVRSTVDLWVERADQQLYRAKADGRNLVFLDQPQESAVSAEEKGLLFSPIAVTE
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHCHHHCCCEECCEEECC
AQPSAENSAGSPA
CCCCCCCCCCCCC
>Mature Secondary Structure 
ATLSSVSPIDLHALRLDDARALLGHAGKPGAPEAGEAAVEYLQRVIDELCELSLKDPLT
CCCCCCCCCCEEEEECCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
GLGNRRQFRAALERAIEIVARSGDSVLLLLLDIDHFKQINDAHGHLVGDQVLQAVARALA
CCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHH
ESIRPMDTVARYGGEEFAMILPNCLPACGHAVAERIRHRIEALSVQVAPHTALRVTASIG
HHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHEEEEHHHC
GAYAPQWVRSTVDLWVERADQQLYRAKADGRNLVFLDQPQESAVSAEEKGLLFSPIAVTE
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHCHHHCCCEECCEEECC
AQPSAENSAGSPA
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA