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The map label for this gene is rimO

Identifier: 74317782

GI number: 74317782

Start: 1856924

End: 1858255

Strand: Reverse

Name: rimO

Synonym: Tbd_1764

Alternate gene names: 74317782

Gene position: 1858255-1856924 (Counterclockwise)

Preceding gene: 74317783

Following gene: 74317780

Centisome position: 63.86

GC content: 64.41

Gene sequence:

>1332_bases
ATGGTAACCAAAGCAAAGACCGCGCGTACACCGACGGTCGGCTTCGTCTCACTGGGCTGTCCCAAGGCCACCGTCGATTC
GGAACGCATCCTCACGCAGCTACGCGCCGAGGGTTATGGCATCGTCGGCAGCTACGACGACGCGGACGTCGTGGTCGTGA
ATACCTGCGGTTTCATCGACGCTGCGGTGCAGGAGTCGCTCGAGGCGATCGGCGAGGCGATTGCGGAGAACGGCAAGGTC
ATCGTCACCGGCTGTCTCGGCGCGAAAGGCGAACTGATACGCGAGGTCCATCCCAAGGTGTTGGCGGTATCGGGGCCGCA
CGCGCTCGACGAGGTGATGGAAGCGGTGCACGGCGCGCTGCCGAAGCCGCACGATCCGTTCGCCGACCTGATCCCGCCGG
GCGGCATCAAGCTCACGCCGCGTCATTATGCCTACCTGAAGATATCCGAAGGCTGCAATCACCGTTGCAGCTTCTGCATC
ATTCCGTCGATGCGGGGCGATCTCGTGAGCCGTCCGATCGGCGAGGTGATGCACGAGGCGGAAGCGCTGGTGAGCGCCGG
CGTGCAGGAGCTCCTCGTCGTCTCGCAGGACACGAGCGCCTACGGAGTCGATGTGAAGTATCGCCCGGGCTTCTGGGGCG
GACGCCCCGTGAAGACGCGCATGACCGAACTCGCGCGCGCCCTCGGGAGTCTCGGCGCCTGGGTGAGGCTGCACTACGTC
TATCCCTATCCGAGCGTCGACGACGTCATTCCGCTGATGGCCGACGGCGTCATTCTGCCTTACCTCGACATTCCCTTTCA
GCATGCGAGCCCACGCATCCTGAAGCTCATGAAGCGGCCCGGCGCGGTCGAAAAAACGCTCGACCGCATCCATGCCTGGC
GGGCCGCCGTGCCCGAGCTTACGCTGCGATCGACGTTCATCGTGGGCTTCCCCGGTGAGACAGACCCCGAGTTCGAGGAA
CTGCTGGATTTCCTCAAGCACGCACAGCTCGACCGCGTCGGCTGCTTCAAATACTCGCCCATCGAGGGCGCCGTTGCCAA
CGAGCTGCCGGACCCCGTTCCCGAAGAACTGAAAGACGAGCGGCTAGAGCGCTTCATGGAAACGCAGGCGGAAATTTCTG
CGGCCCGGCTCGACGCCAAAATCGGCAGGACGATCGAGGTCCTGGTCGATGAGGAGGACGAAGTCGGCACGCTTGCGCGC
AGCCACGCCGACGCCCCCGAGATCGACGGCGTCGTCTATCTGGAGGGCGTCTTCGGTCTGAAGCCCGGGACGCGCCTCAC
GGTGAAAGTCGACGAAGCCGACGCCCACGATCTCTGGGCGACACCGGTCTGA

Upstream 100 bases:

>100_bases
CCTTGCCGACGCTGCTCGCGGTGATCGCCATCATGAGAATCACGTACGCCCAGCCGATCGCCACGATATACATCTCATTG
CTCCATCAGGATTGAACGTC

Downstream 100 bases:

>100_bases
AGCCGCCTACTCGGCAAGCAGCGAGGCGAGGGGACCCGAGCGTCGCCAGACTTGCCATAAGCCCGACGCACGCAGATCGA
GGCCATGGCCGCTGACGGGG

Product: hypothetical protein

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO

Number of amino acids: Translated: 443; Mature: 443

Protein sequence:

>443_residues
MVTKAKTARTPTVGFVSLGCPKATVDSERILTQLRAEGYGIVGSYDDADVVVVNTCGFIDAAVQESLEAIGEAIAENGKV
IVTGCLGAKGELIREVHPKVLAVSGPHALDEVMEAVHGALPKPHDPFADLIPPGGIKLTPRHYAYLKISEGCNHRCSFCI
IPSMRGDLVSRPIGEVMHEAEALVSAGVQELLVVSQDTSAYGVDVKYRPGFWGGRPVKTRMTELARALGSLGAWVRLHYV
YPYPSVDDVIPLMADGVILPYLDIPFQHASPRILKLMKRPGAVEKTLDRIHAWRAAVPELTLRSTFIVGFPGETDPEFEE
LLDFLKHAQLDRVGCFKYSPIEGAVANELPDPVPEELKDERLERFMETQAEISAARLDAKIGRTIEVLVDEEDEVGTLAR
SHADAPEIDGVVYLEGVFGLKPGTRLTVKVDEADAHDLWATPV

Sequences:

>Translated_443_residues
MVTKAKTARTPTVGFVSLGCPKATVDSERILTQLRAEGYGIVGSYDDADVVVVNTCGFIDAAVQESLEAIGEAIAENGKV
IVTGCLGAKGELIREVHPKVLAVSGPHALDEVMEAVHGALPKPHDPFADLIPPGGIKLTPRHYAYLKISEGCNHRCSFCI
IPSMRGDLVSRPIGEVMHEAEALVSAGVQELLVVSQDTSAYGVDVKYRPGFWGGRPVKTRMTELARALGSLGAWVRLHYV
YPYPSVDDVIPLMADGVILPYLDIPFQHASPRILKLMKRPGAVEKTLDRIHAWRAAVPELTLRSTFIVGFPGETDPEFEE
LLDFLKHAQLDRVGCFKYSPIEGAVANELPDPVPEELKDERLERFMETQAEISAARLDAKIGRTIEVLVDEEDEVGTLAR
SHADAPEIDGVVYLEGVFGLKPGTRLTVKVDEADAHDLWATPV
>Mature_443_residues
MVTKAKTARTPTVGFVSLGCPKATVDSERILTQLRAEGYGIVGSYDDADVVVVNTCGFIDAAVQESLEAIGEAIAENGKV
IVTGCLGAKGELIREVHPKVLAVSGPHALDEVMEAVHGALPKPHDPFADLIPPGGIKLTPRHYAYLKISEGCNHRCSFCI
IPSMRGDLVSRPIGEVMHEAEALVSAGVQELLVVSQDTSAYGVDVKYRPGFWGGRPVKTRMTELARALGSLGAWVRLHYV
YPYPSVDDVIPLMADGVILPYLDIPFQHASPRILKLMKRPGAVEKTLDRIHAWRAAVPELTLRSTFIVGFPGETDPEFEE
LLDFLKHAQLDRVGCFKYSPIEGAVANELPDPVPEELKDERLERFMETQAEISAARLDAKIGRTIEVLVDEEDEVGTLAR
SHADAPEIDGVVYLEGVFGLKPGTRLTVKVDEADAHDLWATPV

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI93277076, Length=393, Percent_Identity=27.9898218829517, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI28872784, Length=418, Percent_Identity=25.5980861244019, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI28872782, Length=418, Percent_Identity=25.5980861244019, Blast_Score=127, Evalue=2e-29,
Organism=Escherichia coli, GI1787057, Length=440, Percent_Identity=70.6818181818182, Blast_Score=646, Evalue=0.0,
Organism=Escherichia coli, GI1786882, Length=401, Percent_Identity=29.426433915212, Blast_Score=152, Evalue=5e-38,
Organism=Caenorhabditis elegans, GI17553146, Length=478, Percent_Identity=24.2677824267782, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI71996771, Length=253, Percent_Identity=26.8774703557312, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI21356207, Length=485, Percent_Identity=24.9484536082474, Blast_Score=142, Evalue=3e-34,
Organism=Drosophila melanogaster, GI19922432, Length=404, Percent_Identity=25.7425742574257, Blast_Score=100, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMO_THIDA (Q3SI16)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_315522.1
- ProteinModelPortal:   Q3SI16
- SMR:   Q3SI16
- STRING:   Q3SI16
- GeneID:   3671349
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_1764
- NMPDR:   fig|292415.3.peg.1861
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   KADAPEI
- PhylomeDB:   Q3SI16
- BioCyc:   TDEN292415:TBD_1764-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01865
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01125
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 48211; Mature: 48211

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM; PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTKAKTARTPTVGFVSLGCPKATVDSERILTQLRAEGYGIVGSYDDADVVVVNTCGFID
CCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCEEEECCCCCCEEEEECCCHHH
AAVQESLEAIGEAIAENGKVIVTGCLGAKGELIREVHPKVLAVSGPHALDEVMEAVHGAL
HHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHCCCCEEEECCCHHHHHHHHHHHCCC
PKPHDPFADLIPPGGIKLTPRHYAYLKISEGCNHRCSFCIIPSMRGDLVSRPIGEVMHEA
CCCCCCHHHCCCCCCEEECCCEEEEEEECCCCCCCCCEEEECCCCCHHHHCHHHHHHHHH
EALVSAGVQELLVVSQDTSAYGVDVKYRPGFWGGRPVKTRMTELARALGSLGAWVRLHYV
HHHHHHCHHHHEEEECCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEE
YPYPSVDDVIPLMADGVILPYLDIPFQHASPRILKLMKRPGAVEKTLDRIHAWRAAVPEL
CCCCCHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCE
TLRSTFIVGFPGETDPEFEELLDFLKHAQLDRVGCFKYSPIEGAVANELPDPVPEELKDE
EEEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCEEECCCCCHHHHCCCCCCCHHHHHH
RLERFMETQAEISAARLDAKIGRTIEVLVDEEDEVGTLARSHADAPEIDGVVYLEGVFGL
HHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHCCCCCCCCCEEEEEEEECC
KPGTRLTVKVDEADAHDLWATPV
CCCCEEEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MVTKAKTARTPTVGFVSLGCPKATVDSERILTQLRAEGYGIVGSYDDADVVVVNTCGFID
CCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCEEEECCCCCCEEEEECCCHHH
AAVQESLEAIGEAIAENGKVIVTGCLGAKGELIREVHPKVLAVSGPHALDEVMEAVHGAL
HHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHCCCCEEEECCCHHHHHHHHHHHCCC
PKPHDPFADLIPPGGIKLTPRHYAYLKISEGCNHRCSFCIIPSMRGDLVSRPIGEVMHEA
CCCCCCHHHCCCCCCEEECCCEEEEEEECCCCCCCCCEEEECCCCCHHHHCHHHHHHHHH
EALVSAGVQELLVVSQDTSAYGVDVKYRPGFWGGRPVKTRMTELARALGSLGAWVRLHYV
HHHHHHCHHHHEEEECCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEE
YPYPSVDDVIPLMADGVILPYLDIPFQHASPRILKLMKRPGAVEKTLDRIHAWRAAVPEL
CCCCCHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCE
TLRSTFIVGFPGETDPEFEELLDFLKHAQLDRVGCFKYSPIEGAVANELPDPVPEELKDE
EEEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCEEECCCCCHHHHCCCCCCCHHHHHH
RLERFMETQAEISAARLDAKIGRTIEVLVDEEDEVGTLARSHADAPEIDGVVYLEGVFGL
HHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHCCCCCCCCCEEEEEEEECC
KPGTRLTVKVDEADAHDLWATPV
CCCCEEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA