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The map label for this gene is tig

Identifier: 74317696

GI number: 74317696

Start: 1765926

End: 1767224

Strand: Reverse

Name: tig

Synonym: Tbd_1678

Alternate gene names: 74317696

Gene position: 1767224-1765926 (Counterclockwise)

Preceding gene: 74317698

Following gene: 74317695

Centisome position: 60.73

GC content: 63.97

Gene sequence:

>1299_bases
ATGCAAGGAAATCTTGAAGTTCTCGAAGGTCTGGCCCGGCGCCTGGACATCAGCGTGCCTGTGGATCAGCTGGAAACCGA
GGTGCAGAGCCGCCTCAAGCGCTTGGCCCGCAGCGTCAAGATGCATGGCTTCCGGCCTGGCAAGGCACCGCTCTCGGCGG
TCGCGCGCCAACATGGCGCCGGCGTGCGGCAGGAGGTCCTGGGCGAGACCCTGAAGACGCGCTTCGGCGAGGCCGTGCAG
ACGCACCAGCTCAGAATCGCCGGCTATCCCCGGTTCGAGCCCAAGGCCGACGCGTCGGCAGCGGCGGAAATGACCTTCAG
CGCGAGCTTCGAAGTCTACCCGGAGGTGAAAATCGACGCCCTCGATTCGGCGACGCTGAACCGCCCGGTCGTCGAGCTCG
GTGACGCCGACGTCGCCAAAACCCTCGAGGTGCTGCAGAAGCAGCGCCGCACCTTCGCCACGGCCGCGCGTGCGGCGGCC
GAGGGCGATCTGGTCAAGTTCGACTACCAAGGCACCGTCGACGGCGCGCCTTTCGAAGGCGGCCGAGGCGAGGATTTCGC
CGCGGTGATCGGCGAGGGCCGTCTGCTCAAGGATTTCGAACAGGCTCTGGTTGGCCTCAAGGCCGGCGACAGCAAGGGTT
TTGACCTCACCTTCCCTGCCGAATACGCGGCCCGCGAACTCGCCGACAAGACCGCGCATTTCGAGGTGCAGGTGAAGGAA
GTCCAGGCGCCGGTACTGCCGCCGGTCGATGCGGAATTCGCGGAGGCGCTCGGTGTCGAAGATGGCGACGTCGAGAAACT
CAAGGCCGAAGTGAAGTCAAACCTGGAGCGCGAAGTCAAACGCCGGGTCCAGGCCAAGCTCAAGGAGCAGGCGATGGAGC
TGTTGCTGCAGAAAAGCACGCTCGATCTGCCGCGCAGCCTGGTCGAAATGGAAATGGATCGCCTGCGCCGCATGACCGAA
GCCGACATGCAGTCGCGCGGGGTTCAGTCGATGAAGCTTAGTGCCGATATGTTTACCGGGCAGGCCGAACGGCGCGTCCG
CCTCGGGTTGATCCTTGCCGAGATCGTGCAGGCGAACAAGCTTGCCGCCCAGCCCGAGCAGATACGTAGCCTGATTCAGG
ACCAGGCCCAGAGTTACGAGGAGCCGGATCAGGTGGTCCAGTGGTACTACCAGAGCCCGGAACGGATGCAGGAGATCGAG
TCCCTGGCGCTCGAGGAGAACGTGGTAGCATGGGTCGCAGATCAGGCTACCGTGGTAGATGTGGCAACCTCTTTTGACGA
ACTGATGGGACGTGCCTGA

Upstream 100 bases:

>100_bases
TTCCAGCGCCGCAAGGCGTGGGGGTTCGAGTCCCCCCTTTCGCACCATCGACTTCAACATCAGTTCTCCGCTATTTCAAC
CAATTCAGCCAGGAAGTCTC

Downstream 100 bases:

>100_bases
TGCTATATGATTTCGAACGGACCGCCCCCGAACCGCAGGGGCTCGGCCTGGTGCCGATGGTGGTCGAGCAGAGCGGTCGG
GGCGAGCGCGCCTACGACAT

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MQGNLEVLEGLARRLDISVPVDQLETEVQSRLKRLARSVKMHGFRPGKAPLSAVARQHGAGVRQEVLGETLKTRFGEAVQ
THQLRIAGYPRFEPKADASAAAEMTFSASFEVYPEVKIDALDSATLNRPVVELGDADVAKTLEVLQKQRRTFATAARAAA
EGDLVKFDYQGTVDGAPFEGGRGEDFAAVIGEGRLLKDFEQALVGLKAGDSKGFDLTFPAEYAARELADKTAHFEVQVKE
VQAPVLPPVDAEFAEALGVEDGDVEKLKAEVKSNLEREVKRRVQAKLKEQAMELLLQKSTLDLPRSLVEMEMDRLRRMTE
ADMQSRGVQSMKLSADMFTGQAERRVRLGLILAEIVQANKLAAQPEQIRSLIQDQAQSYEEPDQVVQWYYQSPERMQEIE
SLALEENVVAWVADQATVVDVATSFDELMGRA

Sequences:

>Translated_432_residues
MQGNLEVLEGLARRLDISVPVDQLETEVQSRLKRLARSVKMHGFRPGKAPLSAVARQHGAGVRQEVLGETLKTRFGEAVQ
THQLRIAGYPRFEPKADASAAAEMTFSASFEVYPEVKIDALDSATLNRPVVELGDADVAKTLEVLQKQRRTFATAARAAA
EGDLVKFDYQGTVDGAPFEGGRGEDFAAVIGEGRLLKDFEQALVGLKAGDSKGFDLTFPAEYAARELADKTAHFEVQVKE
VQAPVLPPVDAEFAEALGVEDGDVEKLKAEVKSNLEREVKRRVQAKLKEQAMELLLQKSTLDLPRSLVEMEMDRLRRMTE
ADMQSRGVQSMKLSADMFTGQAERRVRLGLILAEIVQANKLAAQPEQIRSLIQDQAQSYEEPDQVVQWYYQSPERMQEIE
SLALEENVVAWVADQATVVDVATSFDELMGRA
>Mature_432_residues
MQGNLEVLEGLARRLDISVPVDQLETEVQSRLKRLARSVKMHGFRPGKAPLSAVARQHGAGVRQEVLGETLKTRFGEAVQ
THQLRIAGYPRFEPKADASAAAEMTFSASFEVYPEVKIDALDSATLNRPVVELGDADVAKTLEVLQKQRRTFATAARAAA
EGDLVKFDYQGTVDGAPFEGGRGEDFAAVIGEGRLLKDFEQALVGLKAGDSKGFDLTFPAEYAARELADKTAHFEVQVKE
VQAPVLPPVDAEFAEALGVEDGDVEKLKAEVKSNLEREVKRRVQAKLKEQAMELLLQKSTLDLPRSLVEMEMDRLRRMTE
ADMQSRGVQSMKLSADMFTGQAERRVRLGLILAEIVQANKLAAQPEQIRSLIQDQAQSYEEPDQVVQWYYQSPERMQEIE
SLALEENVVAWVADQATVVDVATSFDELMGRA

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain

Homologues:

Organism=Escherichia coli, GI1786640, Length=431, Percent_Identity=39.2111368909513, Blast_Score=333, Evalue=1e-92,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): TIG_THIDA (Q3SI97)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_315436.1
- ProteinModelPortal:   Q3SI97
- SMR:   Q3SI97
- STRING:   Q3SI97
- GeneID:   3673065
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_1678
- NMPDR:   fig|292415.3.peg.1775
- eggNOG:   COG0544
- HOGENOM:   HBG635105
- OMA:   RFIPGFE
- PhylomeDB:   Q3SI97
- BioCyc:   TDEN292415:TBD_1678-MONOMER
- HAMAP:   MF_00303
- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881
- Gene3D:   G3DSA:1.10.3120.10
- Gene3D:   G3DSA:3.30.70.1050
- PIRSF:   PIRSF003095
- TIGRFAMs:   TIGR00115

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N; SSF109998 Trigger_fac_C_bac; SSF102735 Trigger_fac_ribosome-bd_bac

EC number: NA

Molecular weight: Translated: 47815; Mature: 47815

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQGNLEVLEGLARRLDISVPVDQLETEVQSRLKRLARSVKMHGFRPGKAPLSAVARQHGA
CCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCC
GVRQEVLGETLKTRFGEAVQTHQLRIAGYPRFEPKADASAAAEMTFSASFEVYPEVKIDA
CHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCHHHHHEEECCCEEECCCEEEEC
LDSATLNRPVVELGDADVAKTLEVLQKQRRTFATAARAAAEGDLVKFDYQGTVDGAPFEG
CCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCC
GRGEDFAAVIGEGRLLKDFEQALVGLKAGDSKGFDLTFPAEYAARELADKTAHFEVQVKE
CCCCCHHHHHCCCHHHHHHHHHHHCCCCCCCCCCEEECCHHHHHHHHHHHHHHEEEEEEE
VQAPVLPPVDAEFAEALGVEDGDVEKLKAEVKSNLEREVKRRVQAKLKEQAMELLLQKST
CCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDLPRSLVEMEMDRLRRMTEADMQSRGVQSMKLSADMFTGQAERRVRLGLILAEIVQANK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
LAAQPEQIRSLIQDQAQSYEEPDQVVQWYYQSPERMQEIESLALEENVVAWVADQATVVD
HCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEEECCCHHHH
VATSFDELMGRA
HHHHHHHHHCCC
>Mature Secondary Structure
MQGNLEVLEGLARRLDISVPVDQLETEVQSRLKRLARSVKMHGFRPGKAPLSAVARQHGA
CCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCC
GVRQEVLGETLKTRFGEAVQTHQLRIAGYPRFEPKADASAAAEMTFSASFEVYPEVKIDA
CHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCHHHHHEEECCCEEECCCEEEEC
LDSATLNRPVVELGDADVAKTLEVLQKQRRTFATAARAAAEGDLVKFDYQGTVDGAPFEG
CCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCC
GRGEDFAAVIGEGRLLKDFEQALVGLKAGDSKGFDLTFPAEYAARELADKTAHFEVQVKE
CCCCCHHHHHCCCHHHHHHHHHHHCCCCCCCCCCEEECCHHHHHHHHHHHHHHEEEEEEE
VQAPVLPPVDAEFAEALGVEDGDVEKLKAEVKSNLEREVKRRVQAKLKEQAMELLLQKST
CCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDLPRSLVEMEMDRLRRMTEADMQSRGVQSMKLSADMFTGQAERRVRLGLILAEIVQANK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
LAAQPEQIRSLIQDQAQSYEEPDQVVQWYYQSPERMQEIESLALEENVVAWVADQATVVD
HCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEEECCCHHHH
VATSFDELMGRA
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA