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The map label for this gene is flgJ [H]

Identifier: 74317647

GI number: 74317647

Start: 1715795

End: 1716679

Strand: Reverse

Name: flgJ [H]

Synonym: Tbd_1629

Alternate gene names: 74317647

Gene position: 1716679-1715795 (Counterclockwise)

Preceding gene: 74317648

Following gene: 74317646

Centisome position: 59.0

GC content: 71.3

Gene sequence:

>885_bases
GTGAGCATCGGCGGCGCCGACCTTTCCTCGAAGTTCGCGCTCGACGTCCAGGGCGTCAACCAGCTCAAGCTCGACGCCAG
ACAGTCGTCGCCCGAAGCGCTGAAGGCCGCCGCGCGCCAGTTCGAGGCGGTCTTCATGAACATGCTGATGAAGAGCATGC
GCGAGGCGACGCCGCAGGACGGCATGTTCGACAGCGAACAGACGCGCATGTACACCTCCATGCTCGACCAGCAGCTGACG
CAGAATCTGGCGAGCCGCGGCATCGGTCTCGCCGACGTCATGGTGCGCCAGCTTTCGACGGCGCTTGCTGCCCAGGCGCC
CGGCCCCGCCGGCGGCCTCAGCGAGCCGGGGCCCGCGCTCGCGCCGGTTCCTGCGCCGGCACCGCCGCAGCAAGCGCCCG
GCCTGACCCGGGGCGGGGATGCGCCGGCGCACGTGCAGGCCTTCGTGCAGCGCCTGGCGCCCCACGCCGAAGCCGCGAGT
GCCAGCAGCGGCATTCCCGCGCGCTTCATGCTCGGCCAGGCCGCGCTCGAGACCGGCTGGGGTCGTAGCGAAATCCGCGG
CGCCGACGGCCGCAACAGCCACAACCTCTTCGGCATCAAGGCGGGCCCCGGCTGGACCGGCCGCAGCGTCGACGTCGTCA
CGACCGAGTACGTCGACGGCCGGCCGCAGAAGCAGGTCGAGCGCTTCCGCGCCTACGACTCCTACGCCGATTCCTTCCGC
GACTACGCGCGGCTGCTGCGCAACAACGCGCGCTACGCCGACGTCGTCGCGTCCGGGCACGACGCAGCGGGCTTTGCCCA
CGGCCTGCAGCAGGCAGGCTATGCGACCGACCCCGCCTACGCGAGCAAGCTCGTGGGCGTGATCCGGATGCTCGACACCG
CGTGA

Upstream 100 bases:

>100_bases
GGCGCTCAATTCGATCGGCGCGACGCCGCAGGACCTGCTCGCGATCCTGCAGGCCCTAAAGGCTTCGGGCGCGCTGCGTG
CCGAACTCGAGGTGATCTGA

Downstream 100 bases:

>100_bases
CTCAAGAAACAGCCCCGGCAACCGATTCAGCTATCGAGTGGATTCGTATCGTTTTCCCCAACCCTTTCGCAACCCTATAG
ATGGCTACCAGCATTCTCGG

Product: mannosyl-glycoprotein endo-beta-N-acetylglucosamidase

Products: NA

Alternate protein names: Muramidase flgJ [H]

Number of amino acids: Translated: 294; Mature: 293

Protein sequence:

>294_residues
MSIGGADLSSKFALDVQGVNQLKLDARQSSPEALKAAARQFEAVFMNMLMKSMREATPQDGMFDSEQTRMYTSMLDQQLT
QNLASRGIGLADVMVRQLSTALAAQAPGPAGGLSEPGPALAPVPAPAPPQQAPGLTRGGDAPAHVQAFVQRLAPHAEAAS
ASSGIPARFMLGQAALETGWGRSEIRGADGRNSHNLFGIKAGPGWTGRSVDVVTTEYVDGRPQKQVERFRAYDSYADSFR
DYARLLRNNARYADVVASGHDAAGFAHGLQQAGYATDPAYASKLVGVIRMLDTA

Sequences:

>Translated_294_residues
MSIGGADLSSKFALDVQGVNQLKLDARQSSPEALKAAARQFEAVFMNMLMKSMREATPQDGMFDSEQTRMYTSMLDQQLT
QNLASRGIGLADVMVRQLSTALAAQAPGPAGGLSEPGPALAPVPAPAPPQQAPGLTRGGDAPAHVQAFVQRLAPHAEAAS
ASSGIPARFMLGQAALETGWGRSEIRGADGRNSHNLFGIKAGPGWTGRSVDVVTTEYVDGRPQKQVERFRAYDSYADSFR
DYARLLRNNARYADVVASGHDAAGFAHGLQQAGYATDPAYASKLVGVIRMLDTA
>Mature_293_residues
SIGGADLSSKFALDVQGVNQLKLDARQSSPEALKAAARQFEAVFMNMLMKSMREATPQDGMFDSEQTRMYTSMLDQQLTQ
NLASRGIGLADVMVRQLSTALAAQAPGPAGGLSEPGPALAPVPAPAPPQQAPGLTRGGDAPAHVQAFVQRLAPHAEAASA
SSGIPARFMLGQAALETGWGRSEIRGADGRNSHNLFGIKAGPGWTGRSVDVVTTEYVDGRPQKQVERFRAYDSYADSFRD
YARLLRNNARYADVVASGHDAAGFAHGLQQAGYATDPAYASKLVGVIRMLDTA

Specific function: Flagellum-specific muramidase which hydrolyzes the peptidoglycan layer to assemble the rod structure in the periplasmic space [H]

COG id: COG1705

COG function: function code NU; Muramidase (flagellum-specific)

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the glycosyl hydrolase 73 family [H]

Homologues:

Organism=Escherichia coli, GI1787321, Length=305, Percent_Identity=39.0163934426229, Blast_Score=209, Evalue=2e-55,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013377
- InterPro:   IPR000423
- InterPro:   IPR019301
- InterPro:   IPR013338
- InterPro:   IPR002901 [H]

Pfam domain/function: PF01832 Glucosaminidase; PF10135 Rod-binding [H]

EC number: 3.2.1.-

Molecular weight: Translated: 31134; Mature: 31003

Theoretical pI: Translated: 7.87; Mature: 7.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIGGADLSSKFALDVQGVNQLKLDARQSSPEALKAAARQFEAVFMNMLMKSMREATPQD
CCCCCCCCCCCEEEECCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GMFDSEQTRMYTSMLDQQLTQNLASRGIGLADVMVRQLSTALAAQAPGPAGGLSEPGPAL
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
APVPAPAPPQQAPGLTRGGDAPAHVQAFVQRLAPHAEAASASSGIPARFMLGQAALETGW
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHCCCCCCCHHHHHHHHHHHCCC
GRSEIRGADGRNSHNLFGIKAGPGWTGRSVDVVTTEYVDGRPQKQVERFRAYDSYADSFR
CHHHCCCCCCCCCCCEEEEECCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHH
DYARLLRNNARYADVVASGHDAAGFAHGLQQAGYATDPAYASKLVGVIRMLDTA
HHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SIGGADLSSKFALDVQGVNQLKLDARQSSPEALKAAARQFEAVFMNMLMKSMREATPQD
CCCCCCCCCCEEEECCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GMFDSEQTRMYTSMLDQQLTQNLASRGIGLADVMVRQLSTALAAQAPGPAGGLSEPGPAL
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
APVPAPAPPQQAPGLTRGGDAPAHVQAFVQRLAPHAEAASASSGIPARFMLGQAALETGW
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHCCCCCCCHHHHHHHHHHHCCC
GRSEIRGADGRNSHNLFGIKAGPGWTGRSVDVVTTEYVDGRPQKQVERFRAYDSYADSFR
CHHHCCCCCCCCCCCEEEEECCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHH
DYARLLRNNARYADVVASGHDAAGFAHGLQQAGYATDPAYASKLVGVIRMLDTA
HHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]