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The map label for this gene is lexA [H]

Identifier: 74317501

GI number: 74317501

Start: 1570775

End: 1571560

Strand: Reverse

Name: lexA [H]

Synonym: Tbd_1483

Alternate gene names: 74317501

Gene position: 1571560-1570775 (Counterclockwise)

Preceding gene: 74317502

Following gene: 74317498

Centisome position: 54.01

GC content: 65.9

Gene sequence:

>786_bases
ATGCCGCGCTGCTTCATGCGGCTGAAGGCGAGGGGGCTGATCTTTCCGAGTTTCATCATGCGGGTCTCCTGCGATGCACG
GCAGCCGGGCCACCGTGCCTTGGCGTTTTTCGGGTTGGGTACTTATACTGTACGGACATACAGTAACGTACTGTACATGC
ATACAGTATCCGGAGGAAGCCCCATGCGAGACCTGACCCCGCGCCAGGAAGAAATCCTGAACCTCATCCGCGAGTGGATC
GACACCACCGGCTCGCCGCCGACGCGCGCCGAGATCGCGCAGCACTTCGGCTTCAGTTCGCCGAACGCGGCCGAGCAGCA
TCTCAAGACGCTGGCGAAGAAGGGCGCGCTGGAACTCGTGTCGGGGGCCTCGCGCGGCATCCGCCTGCCGGGCGGCGGCG
GCCTTGCGGTCGTCGGCCAGGTCGCGGCGGGCAGTCCGATCCTCGCGCAGGAAAACATCGAGCGGCATGTCCAGGTCGAC
ACCGCGCTGTTCAGCCCGCGCGCGGACTACCTGCTCAAGGTGCGCGGTCAGAGCATGAAGGACATCGGCATCCTCGACGG
CGACCTGCTCGCCGTGCACCGCAGCGCCGAGGCGCGGGCCGGGCAGGTCGTCGTCGCACGCATCGGTGATGAGGTCACGG
TCAAGCGCTTCCAGAAACGCGGCCATACCGTGCAGCTCCTGCCCGAGAACGCCGACTTCGAACCGATCGTCGTCGACCTC
AAGCGCCAGGAGCTCGTCATCGAGGGTATCGCGGTCGGCGTGATCCGCAGCGGCAGGAGCCTCTAA

Upstream 100 bases:

>100_bases
AGGCCCGGGGCTGGGGCTGTCGAGGTAGACCACTTGCGCGCCGTCGAGCTGCTTTTCGACCTGACCGTGGGCGAGGAGGT
CGTTCAGGGCAGCGATCGAA

Downstream 100 bases:

>100_bases
CCGCGGCCAGCCCTACCGCGGCGGCTCCGCTTCGCGCGAGGCGCCGGGTTGACGCTGCTGGCCGTGGCCGCGGGGCGACG
GTTTCCGTCGGCGCGGTAGC

Product: SOS-response transcriptional repressor, LexA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 261; Mature: 260

Protein sequence:

>261_residues
MPRCFMRLKARGLIFPSFIMRVSCDARQPGHRALAFFGLGTYTVRTYSNVLYMHTVSGGSPMRDLTPRQEEILNLIREWI
DTTGSPPTRAEIAQHFGFSSPNAAEQHLKTLAKKGALELVSGASRGIRLPGGGGLAVVGQVAAGSPILAQENIERHVQVD
TALFSPRADYLLKVRGQSMKDIGILDGDLLAVHRSAEARAGQVVVARIGDEVTVKRFQKRGHTVQLLPENADFEPIVVDL
KRQELVIEGIAVGVIRSGRSL

Sequences:

>Translated_261_residues
MPRCFMRLKARGLIFPSFIMRVSCDARQPGHRALAFFGLGTYTVRTYSNVLYMHTVSGGSPMRDLTPRQEEILNLIREWI
DTTGSPPTRAEIAQHFGFSSPNAAEQHLKTLAKKGALELVSGASRGIRLPGGGGLAVVGQVAAGSPILAQENIERHVQVD
TALFSPRADYLLKVRGQSMKDIGILDGDLLAVHRSAEARAGQVVVARIGDEVTVKRFQKRGHTVQLLPENADFEPIVVDL
KRQELVIEGIAVGVIRSGRSL
>Mature_260_residues
PRCFMRLKARGLIFPSFIMRVSCDARQPGHRALAFFGLGTYTVRTYSNVLYMHTVSGGSPMRDLTPRQEEILNLIREWID
TTGSPPTRAEIAQHFGFSSPNAAEQHLKTLAKKGALELVSGASRGIRLPGGGGLAVVGQVAAGSPILAQENIERHVQVDT
ALFSPRADYLLKVRGQSMKDIGILDGDLLAVHRSAEARAGQVVVARIGDEVTVKRFQKRGHTVQLLPENADFEPIVVDLK
RQELVIEGIAVGVIRSGRSL

Specific function: Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, lea

COG id: COG1974

COG function: function code KT; SOS-response transcriptional repressors (RecA-mediated autopeptidases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S24 family [H]

Homologues:

Organism=Escherichia coli, GI1790476, Length=202, Percent_Identity=65.3465346534654, Blast_Score=264, Evalue=5e-72,
Organism=Escherichia coli, GI1787431, Length=120, Percent_Identity=36.6666666666667, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006199
- InterPro:   IPR006200
- InterPro:   IPR006197
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01726 LexA_DNA_bind; PF00717 Peptidase_S24 [H]

EC number: =3.4.21.88 [H]

Molecular weight: Translated: 28457; Mature: 28326

Theoretical pI: Translated: 10.34; Mature: 10.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRCFMRLKARGLIFPSFIMRVSCDARQPGHRALAFFGLGTYTVRTYSNVLYMHTVSGGS
CCHHHHHHHHCCCCHHHHHHHEECCCCCCCCCEEEEEECCCEEEEECCCEEEEEEECCCC
PMRDLTPRQEEILNLIREWIDTTGSPPTRAEIAQHFGFSSPNAAEQHLKTLAKKGALELV
CCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHH
SGASRGIRLPGGGGLAVVGQVAAGSPILAQENIERHVQVDTALFSPRADYLLKVRGQSMK
CCCCCCEECCCCCCEEEEEECCCCCCCEEHHHHHHHEEHHHHHCCCCCCEEEEECCCCCC
DIGILDGDLLAVHRSAEARAGQVVVARIGDEVTVKRFQKRGHTVQLLPENADFEPIVVDL
CCCCCCCCEEEEECCCCCCCCCEEEEECCCHHHHHHHHHCCCEEEEECCCCCCCEEEEEC
KRQELVIEGIAVGVIRSGRSL
CHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PRCFMRLKARGLIFPSFIMRVSCDARQPGHRALAFFGLGTYTVRTYSNVLYMHTVSGGS
CHHHHHHHHCCCCHHHHHHHEECCCCCCCCCEEEEEECCCEEEEECCCEEEEEEECCCC
PMRDLTPRQEEILNLIREWIDTTGSPPTRAEIAQHFGFSSPNAAEQHLKTLAKKGALELV
CCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHH
SGASRGIRLPGGGGLAVVGQVAAGSPILAQENIERHVQVDTALFSPRADYLLKVRGQSMK
CCCCCCEECCCCCCEEEEEECCCCCCCEEHHHHHHHEEHHHHHCCCCCCEEEEECCCCCC
DIGILDGDLLAVHRSAEARAGQVVVARIGDEVTVKRFQKRGHTVQLLPENADFEPIVVDL
CCCCCCCCEEEEECCCCCCCCCEEEEECCCHHHHHHHHHCCCEEEEECCCCCCCEEEEEC
KRQELVIEGIAVGVIRSGRSL
CHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA