Click here to switch to the map view.

The map label for this gene is mexA [H]

Identifier: 74317436

GI number: 74317436

Start: 1508390

End: 1509592

Strand: Reverse

Name: mexA [H]

Synonym: Tbd_1418

Alternate gene names: 74317436

Gene position: 1509592-1508390 (Counterclockwise)

Preceding gene: 74317438

Following gene: 74317435

Centisome position: 51.88

GC content: 70.57

Gene sequence:

>1203_bases
ATGCCCACTCGCTGCAATCTGCCTTCCCCCTCGCTGATCGTCGCGCTCGCGGGCAGCCTGTTTCTTGCCGCGTGCGGCGG
CGACAACGAAGCGCAGAAGGGCGGCCCCGGCGCCGGCGGCCCGGGCGCGCCCGCGGTGACGGTGCTCACGGTGCAGGCCG
AAGCCGTGCCGCTCGCGACCGAACTGCCCGGGCGCACGACGCCTTACATGATCGCGGAGTTGCGGCCGCAGGTGACGGGC
ATCGTCAAGGCGCGGCCATTCAAGGAAGGCAGCGAGGTCAAGGCCGGCCAGGTCTTGTACCGCATCGACCCGGCGCCTTA
TCAGGCCGCGTACAACAGCGCCGTCGCCAACCTTGCGCGCGCCGAGGCCAACGTTGCCGCGGCGCGGACGAAAGCCGAGC
GGTACGCCGAGCTGGTCAAGATCGAGGCCGTGAGCAAACAGGCCAACGACGAGGCCGTCGCGGCGATGAAGCAGGCCCAG
GCCGAAGTCGGCGCCGCGAAGGCGGCGCTCGAAAGCGCGCGCATCGATCTCGGCTTCACTCGCGTCGCGTCGCCGATTTC
GGGACGCATCGGGCGCTCTACCGTCACGCCCGGCGCGCTCGTCACGGCGAACCAGGCGCAGGCGCTGGCGACAGTCCAGC
AGCTCGATCCGATCTATGTCGACCTCACCCAGTCGAGCGCAGAACTGCTGCGCATGCGTCGCGATTTCGCCTCCGGCAAG
CTCAGGCGCAGCGGCAGCGACAGCCTGCCGGTCGAACTCGTGCTCGAAGACGGCAGCGTCTACGGCGCGAAGGGCAGGCT
CGCGTTTTCCGAGGTCGCCGTCGACGAGGCCACCGGCAGCGTCACGCTGCGCGCGGTCTTTCCCAACCCCGACGGCGAGC
TGCTGCCCGGCATGTACGTGCGCGCGCGGCTCTCGCAGGGCGTGCGCGAGGACGCGATCCTCGTTCCGCACGCGGCCGTC
ACGCACGACCCGCGCGGCAACGCGACGGTCATGCTGGTCAATCCGAAAGGCATCGCCGAGGCGCGCGTGGTCAAAGCCGA
GCAGTCGATCCAGGACAAATGGGTCGTGACGGAAGGGCTCGCGCCCGGTGAACGCGTCATCGTCGAGGGACTGCAAAAGG
CCCGGCCGGGTACGCCCGTCCAGGCCCAGCCGGCGGGTCGAGCAGCGCCCGCCGCGACCACGCCGCCGGCAACGAAGGGG
TAG

Upstream 100 bases:

>100_bases
AGATTGAACGTTCATTCTAGAACAGTGCTTCGCCCAGTCACCGGCCTTCGCGGTTTCCGCACGCCGGTTTTCACCCGTCC
CAACGCTTGCAAAGGTCACC

Downstream 100 bases:

>100_bases
TCCGAGATGGCGCGCTTTTTCATCGACCGGCCCATTTTCGCGTGGGTCATCGCCATCGTCATCATGCTCGCCGGCGCGCT
GTCGATCCTGACACTGCCGG

Product: secretion protein HlyD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MPTRCNLPSPSLIVALAGSLFLAACGGDNEAQKGGPGAGGPGAPAVTVLTVQAEAVPLATELPGRTTPYMIAELRPQVTG
IVKARPFKEGSEVKAGQVLYRIDPAPYQAAYNSAVANLARAEANVAAARTKAERYAELVKIEAVSKQANDEAVAAMKQAQ
AEVGAAKAALESARIDLGFTRVASPISGRIGRSTVTPGALVTANQAQALATVQQLDPIYVDLTQSSAELLRMRRDFASGK
LRRSGSDSLPVELVLEDGSVYGAKGRLAFSEVAVDEATGSVTLRAVFPNPDGELLPGMYVRARLSQGVREDAILVPHAAV
THDPRGNATVMLVNPKGIAEARVVKAEQSIQDKWVVTEGLAPGERVIVEGLQKARPGTPVQAQPAGRAAPAATTPPATKG

Sequences:

>Translated_400_residues
MPTRCNLPSPSLIVALAGSLFLAACGGDNEAQKGGPGAGGPGAPAVTVLTVQAEAVPLATELPGRTTPYMIAELRPQVTG
IVKARPFKEGSEVKAGQVLYRIDPAPYQAAYNSAVANLARAEANVAAARTKAERYAELVKIEAVSKQANDEAVAAMKQAQ
AEVGAAKAALESARIDLGFTRVASPISGRIGRSTVTPGALVTANQAQALATVQQLDPIYVDLTQSSAELLRMRRDFASGK
LRRSGSDSLPVELVLEDGSVYGAKGRLAFSEVAVDEATGSVTLRAVFPNPDGELLPGMYVRARLSQGVREDAILVPHAAV
THDPRGNATVMLVNPKGIAEARVVKAEQSIQDKWVVTEGLAPGERVIVEGLQKARPGTPVQAQPAGRAAPAATTPPATKG
>Mature_399_residues
PTRCNLPSPSLIVALAGSLFLAACGGDNEAQKGGPGAGGPGAPAVTVLTVQAEAVPLATELPGRTTPYMIAELRPQVTGI
VKARPFKEGSEVKAGQVLYRIDPAPYQAAYNSAVANLARAEANVAAARTKAERYAELVKIEAVSKQANDEAVAAMKQAQA
EVGAAKAALESARIDLGFTRVASPISGRIGRSTVTPGALVTANQAQALATVQQLDPIYVDLTQSSAELLRMRRDFASGKL
RRSGSDSLPVELVLEDGSVYGAKGRLAFSEVAVDEATGSVTLRAVFPNPDGELLPGMYVRARLSQGVREDAILVPHAAVT
HDPRGNATVMLVNPKGIAEARVVKAEQSIQDKWVVTEGLAPGERVIVEGLQKARPGTPVQAQPAGRAAPAATTPPATKG

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor. Note=The membrane anchor is not necessary for antibiotic efflux as the protein is functional when targeted to the periplasm by a foreign signal peptide [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789665, Length=373, Percent_Identity=54.1554959785523, Blast_Score=368, Evalue=1e-103,
Organism=Escherichia coli, GI1786668, Length=373, Percent_Identity=53.3512064343164, Blast_Score=366, Evalue=1e-102,
Organism=Escherichia coli, GI1789929, Length=339, Percent_Identity=50.7374631268437, Blast_Score=337, Evalue=9e-94,
Organism=Escherichia coli, GI87082036, Length=378, Percent_Identity=28.5714285714286, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI87081951, Length=125, Percent_Identity=34.4, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI87081796, Length=327, Percent_Identity=24.7706422018349, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 41483; Mature: 41352

Theoretical pI: Translated: 9.26; Mature: 9.26

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTRCNLPSPSLIVALAGSLFLAACGGDNEAQKGGPGAGGPGAPAVTVLTVQAEAVPLAT
CCCCCCCCCCHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEEECCEEEHH
ELPGRTTPYMIAELRPQVTGIVKARPFKEGSEVKAGQVLYRIDPAPYQAAYNSAVANLAR
CCCCCCCCEEHEECCCCCEEEEEECCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHH
AEANVAAARTKAERYAELVKIEAVSKQANDEAVAAMKQAQAEVGAAKAALESARIDLGFT
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCHH
RVASPISGRIGRSTVTPGALVTANQAQALATVQQLDPIYVDLTQSSAELLRMRRDFASGK
HHCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHCCC
LRRSGSDSLPVELVLEDGSVYGAKGRLAFSEVAVDEATGSVTLRAVFPNPDGELLPGMYV
CCCCCCCCCCEEEEEECCCEECCCCCEEHHHHHHCCCCCCEEEEEECCCCCCCCCCCHHH
RARLSQGVREDAILVPHAAVTHDPRGNATVMLVNPKGIAEARVVKAEQSIQDKWVVTEGL
HHHHHCCCCCCEEECCCHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCEEEECCC
APGERVIVEGLQKARPGTPVQAQPAGRAAPAATTPPATKG
CCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PTRCNLPSPSLIVALAGSLFLAACGGDNEAQKGGPGAGGPGAPAVTVLTVQAEAVPLAT
CCCCCCCCCHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEEECCEEEHH
ELPGRTTPYMIAELRPQVTGIVKARPFKEGSEVKAGQVLYRIDPAPYQAAYNSAVANLAR
CCCCCCCCEEHEECCCCCEEEEEECCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHH
AEANVAAARTKAERYAELVKIEAVSKQANDEAVAAMKQAQAEVGAAKAALESARIDLGFT
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCHH
RVASPISGRIGRSTVTPGALVTANQAQALATVQQLDPIYVDLTQSSAELLRMRRDFASGK
HHCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHCCC
LRRSGSDSLPVELVLEDGSVYGAKGRLAFSEVAVDEATGSVTLRAVFPNPDGELLPGMYV
CCCCCCCCCCEEEEEECCCEECCCCCEEHHHHHHCCCCCCEEEEEECCCCCCCCCCCHHH
RARLSQGVREDAILVPHAAVTHDPRGNATVMLVNPKGIAEARVVKAEQSIQDKWVVTEGL
HHHHHCCCCCCEEECCCHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCEEEECCC
APGERVIVEGLQKARPGTPVQAQPAGRAAPAATTPPATKG
CCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7968531; 10984043; 9989496; 8226684; 8540696; 9603892; 10671490 [H]