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The map label for this gene is macA [H]

Identifier: 74317432

GI number: 74317432

Start: 1500956

End: 1502248

Strand: Reverse

Name: macA [H]

Synonym: Tbd_1414

Alternate gene names: 74317432

Gene position: 1502248-1500956 (Counterclockwise)

Preceding gene: 74317433

Following gene: 74317431

Centisome position: 51.63

GC content: 71.15

Gene sequence:

>1293_bases
ATGACCCACCCCAACACCGCGCCGGGCGAGCGCAGCACCGCCGTGAAGGCGCTCGTCGACGCGCGCTCGACTCCCTGGAC
GCGCCGGCTCATCGGCGCGGCGCTGATCTTGCTCGTGATCGCCGTGGGCGCGTGGCTGCTTCTCGGGCTCGACGACGATG
AAGACGCCGCGCGCTACAAGACCGCGCCGGTAACCCGTGGCGAGCTCGTCGTCAGGGTGTCCGCGACGGGCAATCTGCAG
CCGACCAACCAGGTCGAGGTCGGCAGCGAGCTGTCGGGCATCGTCGAACGCGTGCTCGTCGACGAGAACGACCGGGTGAA
GCGAGGCCAGGTGCTGGCGCGGCTCGACGTTTCCAAGCTGCGCGACACGGTGACGCGGTCGCGGGCCAACCTCGCCGCGG
CGGAGGCACAGGTGCTGCAGGCCCGGGCGACCGTCGCCGAAGCACGCGCGACTCGCGCGCGCTACCAGCAGGTCGCCGAG
CTCTCGGGCGGCAAGGTGCCATCGAAGAGCGAGATGGACACGGCCGAGGCCAACCTCAAGCGCGCCGAAGCGAACGAGGC
GAGCGCGCGGGCGGCGGTCGTGCAGGCGCGCGCGACGCTGCAGTCGGACGAGACCAACCTCGCCAAGGCGAGCATCCGCT
CGCCGATCGACGGCGTCGTGCTCGCACGCAAGGTCGAGCCGGGCCAGACCGTCGCGGCGTCTTTCCAGGCGCCGGTGCTG
TTCACGCTCGCCGAGGATCTCGCGCGGATGGAACTGCAGGTCGACGTCGACGAGGCCGACGTCGGTCAGGTCGAGATCGG
CCAGAAAGCGACCTTCACCGTCGACGCCTGGCCGGGACGGCTATACGACGCGGTCATCACCCGCGTCGGCTACGGCTCGC
AGGTGAAGGAGGGCGTCGTCTCCTACCTGACCGTGCTCGAGGTCGACAACGACGATCTGAGCCTGCGCCCCGGCATGACC
GGGACGGCCGAGATCACGACGCTGCGGCGGGCCGACGTCTTGCTCGTGCCGAACGCCGCGCTGCGTTTCACGCCGCCGCA
GGCGGGCCCCGCCGAGGAAGGCCAGAGCCGGGGCGTGACAGGCCTGCTGATGCCGCGCCTGCCACGGCAGACGCCGAAGC
AGAAGGAGACGCCCGCGGGCAGTACGCAGCGCGTATGGGTCCTGCGCGACGGCCGGCCGGCTGAAATCGCCGTCAAGGTC
GGCGCGACCGACGGCCGCGTGACCGAAGTCACGGGCGGCGACCTCAAGCCCGGCATGGAAGTCATTACCGAGGCCGAAAG
CGCGGCGCGATGA

Upstream 100 bases:

>100_bases
CGAGCACCCGCGCGGACGGCGTTCTGGCGCTGATCCGGCTATACAAGGCACTCGGCGGCGGATGGTCGCCGGCCGCATCC
GCTTCGCAAGAGGGCCCCGC

Downstream 100 bases:

>100_bases
ACGACGCGCTGATCCGGCTTGCCGGCGTCACCAAGACCTACGGCCACGGGCCCGCCGCGTTCGAGGCGCTCAAGGGAATC
GATCTGACGATCGACGCCGG

Product: secretion protein HlyD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MTHPNTAPGERSTAVKALVDARSTPWTRRLIGAALILLVIAVGAWLLLGLDDDEDAARYKTAPVTRGELVVRVSATGNLQ
PTNQVEVGSELSGIVERVLVDENDRVKRGQVLARLDVSKLRDTVTRSRANLAAAEAQVLQARATVAEARATRARYQQVAE
LSGGKVPSKSEMDTAEANLKRAEANEASARAAVVQARATLQSDETNLAKASIRSPIDGVVLARKVEPGQTVAASFQAPVL
FTLAEDLARMELQVDVDEADVGQVEIGQKATFTVDAWPGRLYDAVITRVGYGSQVKEGVVSYLTVLEVDNDDLSLRPGMT
GTAEITTLRRADVLLVPNAALRFTPPQAGPAEEGQSRGVTGLLMPRLPRQTPKQKETPAGSTQRVWVLRDGRPAEIAVKV
GATDGRVTEVTGGDLKPGMEVITEAESAAR

Sequences:

>Translated_430_residues
MTHPNTAPGERSTAVKALVDARSTPWTRRLIGAALILLVIAVGAWLLLGLDDDEDAARYKTAPVTRGELVVRVSATGNLQ
PTNQVEVGSELSGIVERVLVDENDRVKRGQVLARLDVSKLRDTVTRSRANLAAAEAQVLQARATVAEARATRARYQQVAE
LSGGKVPSKSEMDTAEANLKRAEANEASARAAVVQARATLQSDETNLAKASIRSPIDGVVLARKVEPGQTVAASFQAPVL
FTLAEDLARMELQVDVDEADVGQVEIGQKATFTVDAWPGRLYDAVITRVGYGSQVKEGVVSYLTVLEVDNDDLSLRPGMT
GTAEITTLRRADVLLVPNAALRFTPPQAGPAEEGQSRGVTGLLMPRLPRQTPKQKETPAGSTQRVWVLRDGRPAEIAVKV
GATDGRVTEVTGGDLKPGMEVITEAESAAR
>Mature_429_residues
THPNTAPGERSTAVKALVDARSTPWTRRLIGAALILLVIAVGAWLLLGLDDDEDAARYKTAPVTRGELVVRVSATGNLQP
TNQVEVGSELSGIVERVLVDENDRVKRGQVLARLDVSKLRDTVTRSRANLAAAEAQVLQARATVAEARATRARYQQVAEL
SGGKVPSKSEMDTAEANLKRAEANEASARAAVVQARATLQSDETNLAKASIRSPIDGVVLARKVEPGQTVAASFQAPVLF
TLAEDLARMELQVDVDEADVGQVEIGQKATFTVDAWPGRLYDAVITRVGYGSQVKEGVVSYLTVLEVDNDDLSLRPGMTG
TAEITTLRRADVLLVPNAALRFTPPQAGPAEEGQSRGVTGLLMPRLPRQTPKQKETPAGSTQRVWVLRDGRPAEIAVKVG
ATDGRVTEVTGGDLKPGMEVITEAESAAR

Specific function: Efflux transporter for macrolide antibiotics [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87081796, Length=314, Percent_Identity=31.5286624203822, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1789929, Length=324, Percent_Identity=28.3950617283951, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 45872; Mature: 45740

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHPNTAPGERSTAVKALVDARSTPWTRRLIGAALILLVIAVGAWLLLGLDDDEDAARYK
CCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHEE
TAPVTRGELVVRVSATGNLQPTNQVEVGSELSGIVERVLVDENDRVKRGQVLARLDVSKL
CCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
RDTVTRSRANLAAAEAQVLQARATVAEARATRARYQQVAELSGGKVPSKSEMDTAEANLK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
RAEANEASARAAVVQARATLQSDETNLAKASIRSPIDGVVLARKVEPGQTVAASFQAPVL
HHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCEEEEEECCCCCCEECCCCCCCH
FTLAEDLARMELQVDVDEADVGQVEIGQKATFTVDAWPGRLYDAVITRVGYGSQVKEGVV
HHHHHHHHHEEEEEECCCCCCCCEEECCCEEEEEECCCCHHHHHHHHHCCCCHHHHHHHH
SYLTVLEVDNDDLSLRPGMTGTAEITTLRRADVLLVPNAALRFTPPQAGPAEEGQSRGVT
HEEEEEEECCCCCEECCCCCCCCEEEEEECCCEEEECCCEEEECCCCCCCCCCHHHCCCE
GLLMPRLPRQTPKQKETPAGSTQRVWVLRDGRPAEIAVKVGATDGRVTEVTGGDLKPGME
EEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEEECCCCEEEEECCCCCCCCHH
VITEAESAAR
HHHHHHHHCC
>Mature Secondary Structure 
THPNTAPGERSTAVKALVDARSTPWTRRLIGAALILLVIAVGAWLLLGLDDDEDAARYK
CCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHEE
TAPVTRGELVVRVSATGNLQPTNQVEVGSELSGIVERVLVDENDRVKRGQVLARLDVSKL
CCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
RDTVTRSRANLAAAEAQVLQARATVAEARATRARYQQVAELSGGKVPSKSEMDTAEANLK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
RAEANEASARAAVVQARATLQSDETNLAKASIRSPIDGVVLARKVEPGQTVAASFQAPVL
HHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCEEEEEECCCCCCEECCCCCCCH
FTLAEDLARMELQVDVDEADVGQVEIGQKATFTVDAWPGRLYDAVITRVGYGSQVKEGVV
HHHHHHHHHEEEEEECCCCCCCCEEECCCEEEEEECCCCHHHHHHHHHCCCCHHHHHHHH
SYLTVLEVDNDDLSLRPGMTGTAEITTLRRADVLLVPNAALRFTPPQAGPAEEGQSRGVT
HEEEEEEECCCCCEECCCCCCCCEEEEEECCCEEEECCCEEEECCCCCCCCCCHHHCCCE
GLLMPRLPRQTPKQKETPAGSTQRVWVLRDGRPAEIAVKVGATDGRVTEVTGGDLKPGME
EEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEEECCCCEEEEECCCCCCCCHH
VITEAESAAR
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]