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The map label for this gene is pcrA [H]

Identifier: 74317324

GI number: 74317324

Start: 1384602

End: 1386710

Strand: Direct

Name: pcrA [H]

Synonym: Tbd_1306

Alternate gene names: 74317324

Gene position: 1384602-1386710 (Clockwise)

Preceding gene: 74317321

Following gene: 74317325

Centisome position: 47.58

GC content: 69.42

Gene sequence:

>2109_bases
ATGTCAGAAACCGCCGTCGTGCCGGGCCAGCAGCCGCCCGCGCTCCCTCCCAGCAGCTACCTGTCGCGCCTGAATCCCGA
GCAGCGCCGTGCCGTCGAACACGACGTGGCAGGCGACGCCTGCGCCGGCCCCTTGCTCGTGATCGCCGGCGCAGGCTCGG
GCAAGACCAACGTGCTCGCGCACCGCGTCGCGCATCTCGTCGCGCACGGAGCCGACCCGGCGGCGATCCTGTTGCTGACG
TTTTCGCGGCGGGCGGCCGACGAGATGGTGCGCCGGGTCGAACGCATCCTGCGCCAGGTCGCCGCCGGACGCCCGCGGCT
CGCCGGCGCCAAACTCGCCTGGGCCGGCACGTTTCACAGCATCGGCGCGCGCCTGTTGCGCGAATACGCGCCGCGCATCG
GGCTCGATCCGGCTTTCACGATCCACGACCGCGAAGACTCGGCCGATCTGCTGAATCTGGTGCGCCATGAACTCGGCCTC
GCCGACACCGGGAAACGCTTTCCGCGCAAGAACACCTGTCTCGCGATCTATTCCGCCGCGGTCAACACGCTCGCGCCGCT
CGGCGACGTGCTGCAGCGACGTTATCCGTGGTGCGTCGAGTGGGAAGACGAACTCAAGCGCCTGTTCCTCGCTTACGTCG
AGGCCAAGCAGGCGCAGCACGTGCTCGATTACGACGATCTGCTGCTGTACTGGGGGCAGATCGCCGGGGAGCCGACGCTC
GCGCGCGAGATGGGCGCGCGGTTCAGCCACATTCTCGTCGACGAATATCAGGACACGAACGCGCTGCAGGCGAGCATCGT
GCGGGCACTCAAGCCTGACGGCCGCGGCGTGACCGTGGTCGGCGACGACGCGCAGTCGATCTATGCCTTTCGCGGGGCGA
CCGTGCGCAACATCCTCGATTTCCCCGGGCAGTTCGCGCCGCCCGCGCGCGTCGTCACGCTCGAGCGCAACTACCGCTCG
ACGCAGCCTATCCTCGCCGCGGCCAACGGCGTGATCGCGCGGGCGAGCGAGGGTTACGCCAAGTCGCTGTGGAGCGAGCG
CGAATCGAGCGAGCGGCCGCAGCTCGTCAGCGTGCGCGACGAGGCCGACCAGGCTGCCTTCGTGGTGGAGGAAGTGCTGG
CGCGGCGCGAAGCGGGCATCGCGCTCAAGGCGCAGGCCGTGCTGTTCCGCGCAGCGCAGCACAGCGCGACGCTCGAGATC
GAGCTGAACCGCCGGCAGGTGCCGTTCGTGAAGTTCGGCGGGCTCAAGTTCCTCGAGGCGGCTCACATCAAGGACGTGCT
CGCGGTGCTCCGCTGGCACGAAAATCCCCGCGACCGCGTCGCGGGCTTTCGCGTGCTGCAGCTCATGCCTGGCATCGGAC
CGAAGACCGCGGGGCAGGTGCTCGACGGTCTCGCGCAGGTGCACGACGTCCGGATGGCCTTGCAGGAGGCGCGCGTGCCG
CCGGCCGCCGCGCATGCGTGGGACGACTTTCTCGGGCTGGTGGCCGCCCTCGCGCAGCCCGGCGCGACGTGGCCGGCGGA
GCTCGAAACGATCGGCAGCTGGTATGCACCCCACCTCGAGCGCCTGTATGACGACGCGGCGGTGCGTCAGGGCGACATCA
GCCAGCTGGAACAGATCGCGCGCACCTATCCCTCGCGCCAGCGCTTCCTGACCGAGCTCACCCTCGATCCGCCCGACGCC
ACGAGCGACGAGGCCGGCGTGCCGCTGCGCGACGAGGACTACCTGATCCTGTCGACGATCCATTCGGCGAAGGGTCAGGA
ATGGGCGGCGGTGTACCTGCTGAACGCGGTCGACGGCTGCATTCCCTCGGATCTCGCGACCGGCAGCCCGCAGGAGATCG
AGGAAGAACGGCGGCTGCTCTACGTCGCGCTGACCCGCGCGAAAGACCACCTGCAGGTCGTCGTGCCGCAGCGTTTCTAT
GTCACGCAGCAGTCGGGCTACGGTGACCGCCACGTCTATGCGGGGCGCACACGCTTCATTCCGCCCGGCATGTCGGAGCT
GTTCGACGCCCGCGTGTGGCCGGTGCGCGCGACGGAAACCGCGCCGGCCTGCGCGCCGCCGCCCGAAGTGGTGGAAGTGC
TGTCGAGAATGAAGGCGATGTGGCGCTGA

Upstream 100 bases:

>100_bases
TCGACTCGTGCCTGAAACATAGTATGCGGCGACCCCTGGCGACCCAGGCCCGTCACGCGGGGCGGCTTGCCAGCGGCGGT
CTACTGTATAAAAATACAGC

Downstream 100 bases:

>100_bases
GCGCGCGACGCGCGTTCAACAACTATGCTTCGTTCATGTCCGTTTCATGGGGCGCCCGAAGGGGCCCTCATGAAAGACAC
CCGTATTCTCAGGAGGGCAG

Product: ATP-dependent DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 702; Mature: 701

Protein sequence:

>702_residues
MSETAVVPGQQPPALPPSSYLSRLNPEQRRAVEHDVAGDACAGPLLVIAGAGSGKTNVLAHRVAHLVAHGADPAAILLLT
FSRRAADEMVRRVERILRQVAAGRPRLAGAKLAWAGTFHSIGARLLREYAPRIGLDPAFTIHDREDSADLLNLVRHELGL
ADTGKRFPRKNTCLAIYSAAVNTLAPLGDVLQRRYPWCVEWEDELKRLFLAYVEAKQAQHVLDYDDLLLYWGQIAGEPTL
AREMGARFSHILVDEYQDTNALQASIVRALKPDGRGVTVVGDDAQSIYAFRGATVRNILDFPGQFAPPARVVTLERNYRS
TQPILAAANGVIARASEGYAKSLWSERESSERPQLVSVRDEADQAAFVVEEVLARREAGIALKAQAVLFRAAQHSATLEI
ELNRRQVPFVKFGGLKFLEAAHIKDVLAVLRWHENPRDRVAGFRVLQLMPGIGPKTAGQVLDGLAQVHDVRMALQEARVP
PAAAHAWDDFLGLVAALAQPGATWPAELETIGSWYAPHLERLYDDAAVRQGDISQLEQIARTYPSRQRFLTELTLDPPDA
TSDEAGVPLRDEDYLILSTIHSAKGQEWAAVYLLNAVDGCIPSDLATGSPQEIEEERRLLYVALTRAKDHLQVVVPQRFY
VTQQSGYGDRHVYAGRTRFIPPGMSELFDARVWPVRATETAPACAPPPEVVEVLSRMKAMWR

Sequences:

>Translated_702_residues
MSETAVVPGQQPPALPPSSYLSRLNPEQRRAVEHDVAGDACAGPLLVIAGAGSGKTNVLAHRVAHLVAHGADPAAILLLT
FSRRAADEMVRRVERILRQVAAGRPRLAGAKLAWAGTFHSIGARLLREYAPRIGLDPAFTIHDREDSADLLNLVRHELGL
ADTGKRFPRKNTCLAIYSAAVNTLAPLGDVLQRRYPWCVEWEDELKRLFLAYVEAKQAQHVLDYDDLLLYWGQIAGEPTL
AREMGARFSHILVDEYQDTNALQASIVRALKPDGRGVTVVGDDAQSIYAFRGATVRNILDFPGQFAPPARVVTLERNYRS
TQPILAAANGVIARASEGYAKSLWSERESSERPQLVSVRDEADQAAFVVEEVLARREAGIALKAQAVLFRAAQHSATLEI
ELNRRQVPFVKFGGLKFLEAAHIKDVLAVLRWHENPRDRVAGFRVLQLMPGIGPKTAGQVLDGLAQVHDVRMALQEARVP
PAAAHAWDDFLGLVAALAQPGATWPAELETIGSWYAPHLERLYDDAAVRQGDISQLEQIARTYPSRQRFLTELTLDPPDA
TSDEAGVPLRDEDYLILSTIHSAKGQEWAAVYLLNAVDGCIPSDLATGSPQEIEEERRLLYVALTRAKDHLQVVVPQRFY
VTQQSGYGDRHVYAGRTRFIPPGMSELFDARVWPVRATETAPACAPPPEVVEVLSRMKAMWR
>Mature_701_residues
SETAVVPGQQPPALPPSSYLSRLNPEQRRAVEHDVAGDACAGPLLVIAGAGSGKTNVLAHRVAHLVAHGADPAAILLLTF
SRRAADEMVRRVERILRQVAAGRPRLAGAKLAWAGTFHSIGARLLREYAPRIGLDPAFTIHDREDSADLLNLVRHELGLA
DTGKRFPRKNTCLAIYSAAVNTLAPLGDVLQRRYPWCVEWEDELKRLFLAYVEAKQAQHVLDYDDLLLYWGQIAGEPTLA
REMGARFSHILVDEYQDTNALQASIVRALKPDGRGVTVVGDDAQSIYAFRGATVRNILDFPGQFAPPARVVTLERNYRST
QPILAAANGVIARASEGYAKSLWSERESSERPQLVSVRDEADQAAFVVEEVLARREAGIALKAQAVLFRAAQHSATLEIE
LNRRQVPFVKFGGLKFLEAAHIKDVLAVLRWHENPRDRVAGFRVLQLMPGIGPKTAGQVLDGLAQVHDVRMALQEARVPP
AAAHAWDDFLGLVAALAQPGATWPAELETIGSWYAPHLERLYDDAAVRQGDISQLEQIARTYPSRQRFLTELTLDPPDAT
SDEAGVPLRDEDYLILSTIHSAKGQEWAAVYLLNAVDGCIPSDLATGSPQEIEEERRLLYVALTRAKDHLQVVVPQRFYV
TQQSGYGDRHVYAGRTRFIPPGMSELFDARVWPVRATETAPACAPPPEVVEVLSRMKAMWR

Specific function: Has Both Atpase And Helicase Activities. Unwinds DNA Duplexes With 3' To 5' Polarity With Respect To The Bound Strand And Initiates Unwinding Most Effectively When A Single-Stranded Region Is Present. Involved In The Post-Incision Events Of Nucleotide Exc

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=668, Percent_Identity=33.2335329341317, Blast_Score=278, Evalue=1e-75,
Organism=Escherichia coli, GI48994965, Length=658, Percent_Identity=29.3313069908815, Blast_Score=199, Evalue=4e-52,
Organism=Escherichia coli, GI1787196, Length=356, Percent_Identity=29.7752808988764, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6322369, Length=509, Percent_Identity=26.7190569744597, Blast_Score=138, Evalue=2e-33,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 77425; Mature: 77294

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETAVVPGQQPPALPPSSYLSRLNPEQRRAVEHDVAGDACAGPLLVIAGAGSGKTNVLA
CCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHH
HRVAHLVAHGADPAAILLLTFSRRAADEMVRRVERILRQVAAGRPRLAGAKLAWAGTFHS
HHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHH
IGARLLREYAPRIGLDPAFTIHDREDSADLLNLVRHELGLADTGKRFPRKNTCLAIYSAA
HHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHH
VNTLAPLGDVLQRRYPWCVEWEDELKRLFLAYVEAKQAQHVLDYDDLLLYWGQIAGEPTL
HHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHH
AREMGARFSHILVDEYQDTNALQASIVRALKPDGRGVTVVGDDAQSIYAFRGATVRNILD
HHHHCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHCCCHHHHHHH
FPGQFAPPARVVTLERNYRSTQPILAAANGVIARASEGYAKSLWSERESSERPQLVSVRD
CCCCCCCCCEEEEEECCCCCCCCHHHHCCCCEEECCCHHHHHHHHHHHCCCCCCEEEECC
EADQAAFVVEEVLARREAGIALKAQAVLFRAAQHSATLEIELNRRQVPFVKFGGLKFLEA
HHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHCCCEEEEEEECCCCCCEEEECCCHHHHH
AHIKDVLAVLRWHENPRDRVAGFRVLQLMPGIGPKTAGQVLDGLAQVHDVRMALQEARVP
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
PAAAHAWDDFLGLVAALAQPGATWPAELETIGSWYAPHLERLYDDAAVRQGDISQLEQIA
CHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
RTYPSRQRFLTELTLDPPDATSDEAGVPLRDEDYLILSTIHSAKGQEWAAVYLLNAVDGC
HHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEHHHCCCCCCHHHEEHHHHCCC
IPSDLATGSPQEIEEERRLLYVALTRAKDHLQVVVPQRFYVTQQSGYGDRHVYAGRTRFI
CCCCCCCCCHHHHHHHHHEEEEEECCCCCCEEEEECCEEEEEECCCCCCCEEECCCCCCC
PPGMSELFDARVWPVRATETAPACAPPPEVVEVLSRMKAMWR
CCCHHHHHCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SETAVVPGQQPPALPPSSYLSRLNPEQRRAVEHDVAGDACAGPLLVIAGAGSGKTNVLA
CCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHH
HRVAHLVAHGADPAAILLLTFSRRAADEMVRRVERILRQVAAGRPRLAGAKLAWAGTFHS
HHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHH
IGARLLREYAPRIGLDPAFTIHDREDSADLLNLVRHELGLADTGKRFPRKNTCLAIYSAA
HHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHH
VNTLAPLGDVLQRRYPWCVEWEDELKRLFLAYVEAKQAQHVLDYDDLLLYWGQIAGEPTL
HHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHH
AREMGARFSHILVDEYQDTNALQASIVRALKPDGRGVTVVGDDAQSIYAFRGATVRNILD
HHHHCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHCCCHHHHHHH
FPGQFAPPARVVTLERNYRSTQPILAAANGVIARASEGYAKSLWSERESSERPQLVSVRD
CCCCCCCCCEEEEEECCCCCCCCHHHHCCCCEEECCCHHHHHHHHHHHCCCCCCEEEECC
EADQAAFVVEEVLARREAGIALKAQAVLFRAAQHSATLEIELNRRQVPFVKFGGLKFLEA
HHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHCCCEEEEEEECCCCCCEEEECCCHHHHH
AHIKDVLAVLRWHENPRDRVAGFRVLQLMPGIGPKTAGQVLDGLAQVHDVRMALQEARVP
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
PAAAHAWDDFLGLVAALAQPGATWPAELETIGSWYAPHLERLYDDAAVRQGDISQLEQIA
CHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
RTYPSRQRFLTELTLDPPDATSDEAGVPLRDEDYLILSTIHSAKGQEWAAVYLLNAVDGC
HHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEHHHCCCCCCHHHEEHHHHCCC
IPSDLATGSPQEIEEERRLLYVALTRAKDHLQVVVPQRFYVTQQSGYGDRHVYAGRTRFI
CCCCCCCCCHHHHHHHHHEEEEEECCCCCCEEEEECCEEEEEECCCCCCCEEECCCCCCC
PPGMSELFDARVWPVRATETAPACAPPPEVVEVLSRMKAMWR
CCCHHHHHCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]