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The map label for this gene is sucB [H]

Identifier: 74317207

GI number: 74317207

Start: 1249919

End: 1251058

Strand: Direct

Name: sucB [H]

Synonym: Tbd_1189

Alternate gene names: 74317207

Gene position: 1249919-1251058 (Clockwise)

Preceding gene: 161899034

Following gene: 74317208

Centisome position: 42.96

GC content: 70.96

Gene sequence:

>1140_bases
ATGAAGTTTGAAGTACGCGTGCCCACGCTGTCGGATTCGGTCGCCAGCGGCACGCTTTTGCCGTGGCGCAAGGCGGTCGG
CGACACGGTCGCGCGCGACGAGACGCTGGTCGATCTCGAGACCGACAAGGTCATCCTCGAAATCCCCGCGCCGGCCTCAG
GCACGCTGGTCGAGGTGCGCGCGGTGGGGGGCGCCGAAGTCAGGGCGGACGAAGTCATTGCCCTGATCGAGACTGGCGAG
GCCGCCGCGGCGGGCGCGCGCGAAAACGCGACGCCTGCCGCGCCCGCGCCGGCGCCCCCGGCCGCGACTGCGCCCGCTCG
GGCCGCAGCGCCGGCGGAGTCTCCGTCAGTGGCGGCGCCGCCTCCCCGCGTGGCGACGGCCACCGCGCCGCAGCCGCGGG
TCGCCCCGCCTCCGGCCCGTGCCGCCGCGCAGCCGGGCGAGAGCCGTCGCGAGCCGATGTCGCGTCTGCGCCAGCGCGTG
GCCGAGCGCCTCGTCGCGGCACAGCACACGGCGGCCATGCTCACCACGTTCAACGAAGTGAACATGCAACCCGTGAACGA
ATTGCGCCAGCGCTTCAAGGCCGACTTCGAGGTCCGCCACGGCGTCAAGCTCGGCTACATGTCGTTCTTCGTGCGTGCGG
TCTGCCGGGCGCTCGAAGCCTTCCCGATCGTCAACGCGCGGATCGACGGCAATGACATCGTGTGGCATGGCGACGCCGAC
ATCGGCATCGCGATTTCGAGCCCGCGCGGACTCGTCGTGCCCATCCTGCGCCGCGCGCAGCAGCTCTCGTCGGACGAAAT
CGAACGCGCGATCGCCGACTTCGCGCGGCGCGCACGCGACAGCAAGCTCGCGCTCGAGGAGCTCGCCGGCGGGACGTTCT
CGATCACGAACGGCGGCGTGTTCGGCTCGCTGCTCTCGACCCCGATCCTCAATCCGCCGCAGTCGGCGATTCTCGGCATG
CACACGATCCAGGAAAGGCCGGTCGCCGAGCATGGCCAGGTCGTGATCCGGCCCATGATGTATCTCGCGCTGACCTACGA
CCACCGGCTGATCGACGGCCGCGACGCGGTGCAGTTCCTGGTCGCGGTCAAGGCGGCGCTCGAAGCGCCCGACGCGCCGC
TGCCGGAATACCGCGCATGA

Upstream 100 bases:

>100_bases
TACGCCGGCCGGCCCGCGGCCGCGGCGCCCGCGTCGGGCTACATCTCGCGCCATCGTGCCGAACTTGAAGCGCTGCTGCG
CGACGCCCTGGAGACCTCGT

Downstream 100 bases:

>100_bases
CGCGGCCGGATCAGCGGAGCGAGGCAGATCGGGCGTCTAGCACGCTGCTCGAGGTCGAAGACCTGCATTTCGCGTTCGAT
GGCAAAGCCGTTCTGAAGGG

Product: 2-oxoglutarate dehydrogenase E2 component

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MKFEVRVPTLSDSVASGTLLPWRKAVGDTVARDETLVDLETDKVILEIPAPASGTLVEVRAVGGAEVRADEVIALIETGE
AAAAGARENATPAAPAPAPPAATAPARAAAPAESPSVAAPPPRVATATAPQPRVAPPPARAAAQPGESRREPMSRLRQRV
AERLVAAQHTAAMLTTFNEVNMQPVNELRQRFKADFEVRHGVKLGYMSFFVRAVCRALEAFPIVNARIDGNDIVWHGDAD
IGIAISSPRGLVVPILRRAQQLSSDEIERAIADFARRARDSKLALEELAGGTFSITNGGVFGSLLSTPILNPPQSAILGM
HTIQERPVAEHGQVVIRPMMYLALTYDHRLIDGRDAVQFLVAVKAALEAPDAPLPEYRA

Sequences:

>Translated_379_residues
MKFEVRVPTLSDSVASGTLLPWRKAVGDTVARDETLVDLETDKVILEIPAPASGTLVEVRAVGGAEVRADEVIALIETGE
AAAAGARENATPAAPAPAPPAATAPARAAAPAESPSVAAPPPRVATATAPQPRVAPPPARAAAQPGESRREPMSRLRQRV
AERLVAAQHTAAMLTTFNEVNMQPVNELRQRFKADFEVRHGVKLGYMSFFVRAVCRALEAFPIVNARIDGNDIVWHGDAD
IGIAISSPRGLVVPILRRAQQLSSDEIERAIADFARRARDSKLALEELAGGTFSITNGGVFGSLLSTPILNPPQSAILGM
HTIQERPVAEHGQVVIRPMMYLALTYDHRLIDGRDAVQFLVAVKAALEAPDAPLPEYRA
>Mature_379_residues
MKFEVRVPTLSDSVASGTLLPWRKAVGDTVARDETLVDLETDKVILEIPAPASGTLVEVRAVGGAEVRADEVIALIETGE
AAAAGARENATPAAPAPAPPAATAPARAAAPAESPSVAAPPPRVATATAPQPRVAPPPARAAAQPGESRREPMSRLRQRV
AERLVAAQHTAAMLTTFNEVNMQPVNELRQRFKADFEVRHGVKLGYMSFFVRAVCRALEAFPIVNARIDGNDIVWHGDAD
IGIAISSPRGLVVPILRRAQQLSSDEIERAIADFARRARDSKLALEELAGGTFSITNGGVFGSLLSTPILNPPQSAILGM
HTIQERPVAEHGQVVIRPMMYLALTYDHRLIDGRDAVQFLVAVKAALEAPDAPLPEYRA

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=375, Percent_Identity=47.2, Blast_Score=304, Evalue=8e-83,
Organism=Homo sapiens, GI203098816, Length=259, Percent_Identity=31.2741312741313, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI203098753, Length=259, Percent_Identity=31.2741312741313, Blast_Score=115, Evalue=9e-26,
Organism=Homo sapiens, GI31711992, Length=426, Percent_Identity=25.5868544600939, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI110671329, Length=415, Percent_Identity=23.1325301204819, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI260898739, Length=153, Percent_Identity=33.9869281045752, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI1786946, Length=394, Percent_Identity=45.4314720812183, Blast_Score=342, Evalue=3e-95,
Organism=Escherichia coli, GI1786305, Length=239, Percent_Identity=28.8702928870293, Blast_Score=114, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI25146366, Length=395, Percent_Identity=42.7848101265823, Blast_Score=303, Evalue=8e-83,
Organism=Caenorhabditis elegans, GI17560088, Length=427, Percent_Identity=26.6978922716628, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17537937, Length=421, Percent_Identity=22.5653206650831, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17538894, Length=226, Percent_Identity=30.9734513274336, Blast_Score=110, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6320352, Length=395, Percent_Identity=41.7721518987342, Blast_Score=307, Evalue=1e-84,
Organism=Saccharomyces cerevisiae, GI6324258, Length=226, Percent_Identity=30.0884955752212, Blast_Score=109, Evalue=8e-25,
Organism=Drosophila melanogaster, GI24645909, Length=241, Percent_Identity=52.2821576763486, Blast_Score=259, Evalue=1e-69,
Organism=Drosophila melanogaster, GI18859875, Length=260, Percent_Identity=29.6153846153846, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI20129315, Length=223, Percent_Identity=28.2511210762332, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24582497, Length=223, Percent_Identity=28.2511210762332, Blast_Score=99, Evalue=4e-21,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 40377; Mature: 40377

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFEVRVPTLSDSVASGTLLPWRKAVGDTVARDETLVDLETDKVILEIPAPASGTLVEVR
CEEEEECCCCCCHHCCCCCCHHHHHHCCHHHCCCEEEEECCCEEEEEECCCCCCCEEEEE
AVGGAEVRADEVIALIETGEAAAAGARENATPAAPAPAPPAATAPARAAAPAESPSVAAP
ECCCCCCCCCCEEEEEECCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PPRVATATAPQPRVAPPPARAAAQPGESRREPMSRLRQRVAERLVAAQHTAAMLTTFNEV
CCCCEECCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NMQPVNELRQRFKADFEVRHGVKLGYMSFFVRAVCRALEAFPIVNARIDGNDIVWHGDAD
CCCHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEECCCC
IGIAISSPRGLVVPILRRAQQLSSDEIERAIADFARRARDSKLALEELAGGTFSITNGGV
EEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCHHHHHHHHHCCCEEEECCCCH
FGSLLSTPILNPPQSAILGMHTIQERPVAEHGQVVIRPMMYLALTYDHRLIDGRDAVQFL
HHHHHHCCCCCCCHHHHHHHHHHHCCCCHHCCCCHHHHHHHHHHHHCCCEECCHHHHHHH
VAVKAALEAPDAPLPEYRA
HHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MKFEVRVPTLSDSVASGTLLPWRKAVGDTVARDETLVDLETDKVILEIPAPASGTLVEVR
CEEEEECCCCCCHHCCCCCCHHHHHHCCHHHCCCEEEEECCCEEEEEECCCCCCCEEEEE
AVGGAEVRADEVIALIETGEAAAAGARENATPAAPAPAPPAATAPARAAAPAESPSVAAP
ECCCCCCCCCCEEEEEECCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PPRVATATAPQPRVAPPPARAAAQPGESRREPMSRLRQRVAERLVAAQHTAAMLTTFNEV
CCCCEECCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NMQPVNELRQRFKADFEVRHGVKLGYMSFFVRAVCRALEAFPIVNARIDGNDIVWHGDAD
CCCHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEECCCC
IGIAISSPRGLVVPILRRAQQLSSDEIERAIADFARRARDSKLALEELAGGTFSITNGGV
EEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCHHHHHHHHHCCCEEEECCCCH
FGSLLSTPILNPPQSAILGMHTIQERPVAEHGQVVIRPMMYLALTYDHRLIDGRDAVQFL
HHHHHHCCCCCCCHHHHHHHHHHHCCCCHHCCCCHHHHHHHHHHHHCCCEECCHHHHHHH
VAVKAALEAPDAPLPEYRA
HHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]