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The map label for this gene is iscS [H]

Identifier: 74317182

GI number: 74317182

Start: 1217940

End: 1219148

Strand: Direct

Name: iscS [H]

Synonym: Tbd_1164

Alternate gene names: 74317182

Gene position: 1217940-1219148 (Clockwise)

Preceding gene: 74317181

Following gene: 74317183

Centisome position: 41.86

GC content: 64.52

Gene sequence:

>1209_bases
ATGAAAACGATTTACTTCGACTATTCCGCCACCACTCCGGTCGATCCGCGCGTCGCCGACAAGATGATCCCGTACCTGAC
CGAGCACTTCGGCAATCCGGCGTCGCGCTCGCATCCATTCGGCTGGGAAGCCGAGGCTGCCGTCGAGGCGGCGCGCGGCC
ATATCGCGGCGCTGGTGGGCGCGGACCCGAAGGAAATCGTGTTCACCTCGGGCGCCACCGAGTCTAACAACCTCGCCATC
AAGGGCGCCGGCCATTTCTACTCGGGCACCAAGGGCAAGCACATCATCACCGTCCGCACCGAGCACAAGGCCGTGCTCGA
CACCGTGCGCGAGATGGAGCGCCAGGGTTTCGAGGCGACTTATCTCAACGTCAAGGAAAACGGCCTGCTCGACCTCGACG
AGTTTCGCGCCGCGATCCGTCCCGACACGGTGCTGGCGTCGGTGATGGCGGTCAACAACGAGATCGGCGTGATCCAGGAT
ATCGACGCGCTCGGCAAGATCTGCCGCGAGAAGGGCGTGGTGTTCCACGTCGACGCGGCGCAGGCGACCGGCAAAATGCC
GATCGACCTGAAGACGCTGCCGGTCGATCTGATGTCGTTCTCGGCGCACAAGACCTACGGCCCCAAGGGCATCGGCGCCC
TGTACGTGCGCCGCAAGCCGCGCATCCGCCTCGAGGCCCAGATGCACGGCGGCGGGCACGAGCGCGGCATGCGTTCGGGC
ACGCTCGCGACGCACCAGATCGTCGGCATGGGTGAAGCCTTCCGCATCGCCCGCGAGGAAATGGCGACCGAGAACGAGCG
TATCCGCATGCTCCGCGACCGCCTCTATGCGGGCCTCAAGGATATCGAGGAAGTGCACCTGAACGGCGACCTCGATCGCC
GCGTGCCGCACAACCTCAACGTCAGCTTCAACTTCGTCGAGGGCGAGTCGCTGATCATGGCGATCAAGGACCTGGCTGTC
TCGTCGGGTTCGGCCTGCACCTCGGCGAGCCTCGAGCCGTCCTACGTGTTGCGCGCGCTCGGCCGCAGCGACGAGCTCGC
GCACAGCTCGATCCGCTTCTCGATCGGCCGCTTCACGACCGAGCAGGAAGTGGACTACGCGATCAAGCTCCTGCACGAGA
AGATCGGCAAGCTGCGCGAACTGTCCCCGCTGTGGGAAATGTTCAAGGAAGGCATCGATCTCAATACTGTGCAGTGGGCG
GCACACTGA

Upstream 100 bases:

>100_bases
CTGGTCGCCAAGCAGGACGAGTGCAAGGAAAAGGTATTGCAGGATCGTCGCGCGTCGAAAATGACGCTCGCGGTTTGAAA
CGATAGATTAAGGTCAAACG

Downstream 100 bases:

>100_bases
TTTTTTGGGGGTCGGGATTCAGGGATCAGGATTCAGAGGAGGTGCGGTCGGTGGCCGCACCAAGTTAGAAAGCGCGAAGC
ACCGCGCCCGACCCCTGGCC

Product: cysteine desulfurase IscS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MKTIYFDYSATTPVDPRVADKMIPYLTEHFGNPASRSHPFGWEAEAAVEAARGHIAALVGADPKEIVFTSGATESNNLAI
KGAGHFYSGTKGKHIITVRTEHKAVLDTVREMERQGFEATYLNVKENGLLDLDEFRAAIRPDTVLASVMAVNNEIGVIQD
IDALGKICREKGVVFHVDAAQATGKMPIDLKTLPVDLMSFSAHKTYGPKGIGALYVRRKPRIRLEAQMHGGGHERGMRSG
TLATHQIVGMGEAFRIAREEMATENERIRMLRDRLYAGLKDIEEVHLNGDLDRRVPHNLNVSFNFVEGESLIMAIKDLAV
SSGSACTSASLEPSYVLRALGRSDELAHSSIRFSIGRFTTEQEVDYAIKLLHEKIGKLRELSPLWEMFKEGIDLNTVQWA
AH

Sequences:

>Translated_402_residues
MKTIYFDYSATTPVDPRVADKMIPYLTEHFGNPASRSHPFGWEAEAAVEAARGHIAALVGADPKEIVFTSGATESNNLAI
KGAGHFYSGTKGKHIITVRTEHKAVLDTVREMERQGFEATYLNVKENGLLDLDEFRAAIRPDTVLASVMAVNNEIGVIQD
IDALGKICREKGVVFHVDAAQATGKMPIDLKTLPVDLMSFSAHKTYGPKGIGALYVRRKPRIRLEAQMHGGGHERGMRSG
TLATHQIVGMGEAFRIAREEMATENERIRMLRDRLYAGLKDIEEVHLNGDLDRRVPHNLNVSFNFVEGESLIMAIKDLAV
SSGSACTSASLEPSYVLRALGRSDELAHSSIRFSIGRFTTEQEVDYAIKLLHEKIGKLRELSPLWEMFKEGIDLNTVQWA
AH
>Mature_402_residues
MKTIYFDYSATTPVDPRVADKMIPYLTEHFGNPASRSHPFGWEAEAAVEAARGHIAALVGADPKEIVFTSGATESNNLAI
KGAGHFYSGTKGKHIITVRTEHKAVLDTVREMERQGFEATYLNVKENGLLDLDEFRAAIRPDTVLASVMAVNNEIGVIQD
IDALGKICREKGVVFHVDAAQATGKMPIDLKTLPVDLMSFSAHKTYGPKGIGALYVRRKPRIRLEAQMHGGGHERGMRSG
TLATHQIVGMGEAFRIAREEMATENERIRMLRDRLYAGLKDIEEVHLNGDLDRRVPHNLNVSFNFVEGESLIMAIKDLAV
SSGSACTSASLEPSYVLRALGRSDELAHSSIRFSIGRFTTEQEVDYAIKLLHEKIGKLRELSPLWEMFKEGIDLNTVQWA
AH

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=403, Percent_Identity=57.5682382133995, Blast_Score=510, Evalue=1e-145,
Organism=Homo sapiens, GI156713448, Length=416, Percent_Identity=33.6538461538462, Blast_Score=201, Evalue=8e-52,
Organism=Escherichia coli, GI48994898, Length=401, Percent_Identity=73.0673316708229, Blast_Score=625, Evalue=1e-180,
Organism=Escherichia coli, GI1789175, Length=229, Percent_Identity=27.0742358078603, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787970, Length=215, Percent_Identity=27.4418604651163, Blast_Score=78, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI25143064, Length=402, Percent_Identity=56.7164179104478, Blast_Score=492, Evalue=1e-139,
Organism=Caenorhabditis elegans, GI17533177, Length=327, Percent_Identity=32.4159021406728, Blast_Score=149, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6319831, Length=401, Percent_Identity=59.3516209476309, Blast_Score=509, Evalue=1e-145,
Organism=Drosophila melanogaster, GI20129463, Length=402, Percent_Identity=56.9651741293532, Blast_Score=486, Evalue=1e-137,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 44495; Mature: 44495

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTIYFDYSATTPVDPRVADKMIPYLTEHFGNPASRSHPFGWEAEAAVEAARGHIAALVG
CCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCEEEEEC
ADPKEIVFTSGATESNNLAIKGAGHFYSGTKGKHIITVRTEHKAVLDTVREMERQGFEAT
CCCHHEEEECCCCCCCCEEEEECCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHCCCEEE
YLNVKENGLLDLDEFRAAIRPDTVLASVMAVNNEIGVIQDIDALGKICREKGVVFHVDAA
EEEECCCCCEEHHHHHHHCCCHHHHHHHHHHCCCCCCEECHHHHHHHHHHCCCEEEEEHH
QATGKMPIDLKTLPVDLMSFSAHKTYGPKGIGALYVRRKPRIRLEAQMHGGGHERGMRSG
HCCCCCCEEEEECCHHHHHCCCCCCCCCCCCEEEEEECCCCEEEEEEECCCCHHCCCCCC
TLATHQIVGMGEAFRIAREEMATENERIRMLRDRLYAGLKDIEEVHLNGDLDRRVPHNLN
CCHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCC
VSFNFVEGESLIMAIKDLAVSSGSACTSASLEPSYVLRALGRSDELAHSSIRFSIGRFTT
EEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHEEECCCCCC
EQEVDYAIKLLHEKIGKLRELSPLWEMFKEGIDLNTVQWAAH
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCEEEECC
>Mature Secondary Structure
MKTIYFDYSATTPVDPRVADKMIPYLTEHFGNPASRSHPFGWEAEAAVEAARGHIAALVG
CCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCEEEEEC
ADPKEIVFTSGATESNNLAIKGAGHFYSGTKGKHIITVRTEHKAVLDTVREMERQGFEAT
CCCHHEEEECCCCCCCCEEEEECCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHCCCEEE
YLNVKENGLLDLDEFRAAIRPDTVLASVMAVNNEIGVIQDIDALGKICREKGVVFHVDAA
EEEECCCCCEEHHHHHHHCCCHHHHHHHHHHCCCCCCEECHHHHHHHHHHCCCEEEEEHH
QATGKMPIDLKTLPVDLMSFSAHKTYGPKGIGALYVRRKPRIRLEAQMHGGGHERGMRSG
HCCCCCCEEEEECCHHHHHCCCCCCCCCCCCEEEEEECCCCEEEEEEECCCCHHCCCCCC
TLATHQIVGMGEAFRIAREEMATENERIRMLRDRLYAGLKDIEEVHLNGDLDRRVPHNLN
CCHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCC
VSFNFVEGESLIMAIKDLAVSSGSACTSASLEPSYVLRALGRSDELAHSSIRFSIGRFTT
EEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHEEECCCCCC
EQEVDYAIKLLHEKIGKLRELSPLWEMFKEGIDLNTVQWAAH
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA