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The map label for this gene is nuoB [H]

Identifier: 74317161

GI number: 74317161

Start: 1197422

End: 1197898

Strand: Direct

Name: nuoB [H]

Synonym: Tbd_1143

Alternate gene names: 74317161

Gene position: 1197422-1197898 (Clockwise)

Preceding gene: 74317160

Following gene: 74317162

Centisome position: 41.15

GC content: 63.94

Gene sequence:

>477_bases
ATGAGCATCGAGGGCGTCCTCGAGCAGGGCTTCGTCACCACCAAGGCGGACCAGCTCATCAACTACATGCGCACGGGGTC
GATGTGGCCGATGACCTTCGGTCTTGCCTGCTGCGCGGTCGAGATGATGCAGGCCGGCGCCTCGCGCTACGACCTCGATC
GTTTCGGCATCGTCTTTCGTCCGAGCCCGCGGCAGTCCGATGTGATGATCGTCGCCGGCACGCTCTGCAACAAGATGGCT
CCGGCCCTGCGCAAGGTCTACGACCAGATGGCGGAGCCGCGCTGGGTGATCTCGATGGGCTCGTGCGCGAACGGCGGTGG
CTATTATCACTATTCGTATTCGGTCGTGCGCGGGTGCGACCGCATCGTGCCGGTCGACATTTACGTGCCCGGGTGCCCGC
CGACGGCCGAGGCGCTCATATTCGGGATCATCCAGTTGCAGAAGAAGATCCGCCGCACCAACACCATCGCCCGCTGA

Upstream 100 bases:

>100_bases
GTGTTTCTCGGCATCCTCGTCGTCGGTTTCATCTACGAATGGATGAAGGGAGCGCTGGAGTGGGAGTAAGGCCTGTGAGC
CCCAGGGCGAGGCGTGGCGC

Downstream 100 bases:

>100_bases
CATGTCGATCACGCTGGAGTCCTTGTCCGCCTGCCTGAAAAATGCCCTCGGTGACGCGCTCGTTCAGACGATCGAGCGCC
TCGGCGAACTGACGCTCGTG

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B [H]

Number of amino acids: Translated: 158; Mature: 157

Protein sequence:

>158_residues
MSIEGVLEQGFVTTKADQLINYMRTGSMWPMTFGLACCAVEMMQAGASRYDLDRFGIVFRPSPRQSDVMIVAGTLCNKMA
PALRKVYDQMAEPRWVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALIFGIIQLQKKIRRTNTIAR

Sequences:

>Translated_158_residues
MSIEGVLEQGFVTTKADQLINYMRTGSMWPMTFGLACCAVEMMQAGASRYDLDRFGIVFRPSPRQSDVMIVAGTLCNKMA
PALRKVYDQMAEPRWVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALIFGIIQLQKKIRRTNTIAR
>Mature_157_residues
SIEGVLEQGFVTTKADQLINYMRTGSMWPMTFGLACCAVEMMQAGASRYDLDRFGIVFRPSPRQSDVMIVAGTLCNKMAP
ALRKVYDQMAEPRWVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALIFGIIQLQKKIRRTNTIAR

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cyto

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=146, Percent_Identity=78.0821917808219, Blast_Score=254, Evalue=3e-68,
Organism=Escherichia coli, GI1788624, Length=149, Percent_Identity=48.993288590604, Blast_Score=160, Evalue=5e-41,
Organism=Escherichia coli, GI1789074, Length=153, Percent_Identity=35.2941176470588, Blast_Score=99, Evalue=1e-22,
Organism=Escherichia coli, GI1788834, Length=126, Percent_Identity=38.8888888888889, Blast_Score=91, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17509685, Length=142, Percent_Identity=69.7183098591549, Blast_Score=235, Evalue=7e-63,
Organism=Drosophila melanogaster, GI18859983, Length=142, Percent_Identity=78.169014084507, Blast_Score=253, Evalue=3e-68,
Organism=Drosophila melanogaster, GI24642371, Length=142, Percent_Identity=78.169014084507, Blast_Score=253, Evalue=3e-68,
Organism=Drosophila melanogaster, GI24651058, Length=146, Percent_Identity=76.027397260274, Blast_Score=249, Evalue=6e-67,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 17561; Mature: 17430

Theoretical pI: Translated: 8.79; Mature: 8.79

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
6.3 %Met     (Translated Protein)
10.1 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
5.7 %Met     (Mature Protein)
9.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIEGVLEQGFVTTKADQLINYMRTGSMWPMTFGLACCAVEMMQAGASRYDLDRFGIVFR
CCCCHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHCCEEEC
PSPRQSDVMIVAGTLCNKMAPALRKVYDQMAEPRWVISMGSCANGGGYYHYSYSVVRGCD
CCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEHHHHHHCCC
RIVPVDIYVPGCPPTAEALIFGIIQLQKKIRRTNTIAR
CEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SIEGVLEQGFVTTKADQLINYMRTGSMWPMTFGLACCAVEMMQAGASRYDLDRFGIVFR
CCCHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHCCEEEC
PSPRQSDVMIVAGTLCNKMAPALRKVYDQMAEPRWVISMGSCANGGGYYHYSYSVVRGCD
CCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEHHHHHHCCC
RIVPVDIYVPGCPPTAEALIFGIIQLQKKIRRTNTIAR
CEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA