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The map label for this gene is rlmE

Identifier: 74317150

GI number: 74317150

Start: 1186875

End: 1187537

Strand: Direct

Name: rlmE

Synonym: Tbd_1132

Alternate gene names: 74317150

Gene position: 1186875-1187537 (Clockwise)

Preceding gene: 74317148

Following gene: 74317151

Centisome position: 40.79

GC content: 63.8

Gene sequence:

>663_bases
ATGGCGCAGAAACCCAAGCGGACGAAATCGGGCAGCGCGTGGATGCACGAGCACGTGACCGACGCCTATGTCAAAAAGGC
CCAGCAAGACGGCTTCCGTTCCCGCGCGGCCTACAAGCTGCTCGAAATCGACAGCCGCGACCACCTGTTGCATCCTGGAA
TGACGGTCGTCGACCTCGGTGCGGCGCCGGGCAGCTGGTGCCAGGTCGCCGTACAGAAAATGAAGCGGCAGGGCAGGGTG
CTCGCGATCGACCTCCTGCCGGTCGCGCCGCTGCCCGGGGTCGAGGCGCTGCAGGGCGACTTCACCGCGCCCGATACCCT
CGCATGGCTCGAAAATACCCTGCAGGCCGCGCGCGTCGACCTTGTTTTGAGCGACATGGCCCCCAATATGAGCGGGGTGA
TGCTGCGTGACCAGGCGCGCCACTACGAATTATGCGAGTTGGCGCTCGATTTCGCCGTAAATTGGCTGAAACCCGACGGC
GCTTTTCTCGTCAAAGTGTTCCAGGGGAGCGGTTTCGAGGACTTTCGAAACGCGATGCGTCGAGCGTTCGACCAGGTGGT
CATCCGTAAACCCGATGCGTCGCGCGACCGCAGCAGCGAGGTCTACCTTCTGGGGCGCCGTCCGGTTAAGCTGGAGTCCG
TCGCAGCCACGGGCGCTTCGTGA

Upstream 100 bases:

>100_bases
GTGAGCGAGGCCTCGCAGGTATTGGCGCTGCGCGGGGGTGAGTGGTTTCATGGGTATTCCTTAAATCGAGCGCGGATTGT
AGCCGATACGACCTGAAGAA

Downstream 100 bases:

>100_bases
CAGGCTTCCGAAGTGGGCTTAGAATGAGTCTAAACTGCTGCGCAGAATTTGAACGTGCCGTGTTCGGCACCAAGGAGTGA
ACGTGAACAACTTGTCGAAG

Product: cell division protein FtsJ

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase

Number of amino acids: Translated: 220; Mature: 219

Protein sequence:

>220_residues
MAQKPKRTKSGSAWMHEHVTDAYVKKAQQDGFRSRAAYKLLEIDSRDHLLHPGMTVVDLGAAPGSWCQVAVQKMKRQGRV
LAIDLLPVAPLPGVEALQGDFTAPDTLAWLENTLQAARVDLVLSDMAPNMSGVMLRDQARHYELCELALDFAVNWLKPDG
AFLVKVFQGSGFEDFRNAMRRAFDQVVIRKPDASRDRSSEVYLLGRRPVKLESVAATGAS

Sequences:

>Translated_220_residues
MAQKPKRTKSGSAWMHEHVTDAYVKKAQQDGFRSRAAYKLLEIDSRDHLLHPGMTVVDLGAAPGSWCQVAVQKMKRQGRV
LAIDLLPVAPLPGVEALQGDFTAPDTLAWLENTLQAARVDLVLSDMAPNMSGVMLRDQARHYELCELALDFAVNWLKPDG
AFLVKVFQGSGFEDFRNAMRRAFDQVVIRKPDASRDRSSEVYLLGRRPVKLESVAATGAS
>Mature_219_residues
AQKPKRTKSGSAWMHEHVTDAYVKKAQQDGFRSRAAYKLLEIDSRDHLLHPGMTVVDLGAAPGSWCQVAVQKMKRQGRVL
AIDLLPVAPLPGVEALQGDFTAPDTLAWLENTLQAARVDLVLSDMAPNMSGVMLRDQARHYELCELALDFAVNWLKPDGA
FLVKVFQGSGFEDFRNAMRRAFDQVVIRKPDASRDRSSEVYLLGRRPVKLESVAATGAS

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family

Homologues:

Organism=Homo sapiens, GI7019377, Length=208, Percent_Identity=40.8653846153846, Blast_Score=150, Evalue=7e-37,
Organism=Homo sapiens, GI29029591, Length=184, Percent_Identity=38.0434782608696, Blast_Score=124, Evalue=4e-29,
Organism=Homo sapiens, GI29029589, Length=184, Percent_Identity=38.0434782608696, Blast_Score=124, Evalue=4e-29,
Organism=Homo sapiens, GI7110661, Length=184, Percent_Identity=38.0434782608696, Blast_Score=124, Evalue=5e-29,
Organism=Homo sapiens, GI194097365, Length=184, Percent_Identity=34.2391304347826, Blast_Score=121, Evalue=6e-28,
Organism=Escherichia coli, GI1789569, Length=205, Percent_Identity=49.7560975609756, Blast_Score=202, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI17553474, Length=209, Percent_Identity=36.3636363636364, Blast_Score=128, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17554650, Length=187, Percent_Identity=35.2941176470588, Blast_Score=124, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI71987561, Length=196, Percent_Identity=35.7142857142857, Blast_Score=118, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI71987550, Length=196, Percent_Identity=35.7142857142857, Blast_Score=118, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17553860, Length=196, Percent_Identity=35.7142857142857, Blast_Score=117, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6319535, Length=200, Percent_Identity=36, Blast_Score=125, Evalue=6e-30,
Organism=Saccharomyces cerevisiae, GI6319796, Length=185, Percent_Identity=32.4324324324324, Blast_Score=116, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6321302, Length=102, Percent_Identity=37.2549019607843, Blast_Score=80, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21356387, Length=217, Percent_Identity=39.63133640553, Blast_Score=142, Evalue=2e-34,
Organism=Drosophila melanogaster, GI18859957, Length=199, Percent_Identity=33.1658291457286, Blast_Score=111, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24647580, Length=194, Percent_Identity=33.5051546391753, Blast_Score=106, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24648639, Length=194, Percent_Identity=30.4123711340206, Blast_Score=102, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLME_THIDA (Q3SJR5)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_314890.1
- ProteinModelPortal:   Q3SJR5
- SMR:   Q3SJR5
- STRING:   Q3SJR5
- GeneID:   3673203
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_1132
- NMPDR:   fig|292415.3.peg.1131
- eggNOG:   COG0293
- HOGENOM:   HBG398270
- OMA:   SDMAANT
- PhylomeDB:   Q3SJR5
- BioCyc:   TDEN292415:TBD_1132-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01547
- InterPro:   IPR015507
- InterPro:   IPR002877
- PANTHER:   PTHR10920
- PIRSF:   PIRSF005461

Pfam domain/function: PF01728 FtsJ

EC number: =2.1.1.166

Molecular weight: Translated: 24376; Mature: 24245

Theoretical pI: Translated: 9.12; Mature: 9.12

Prosite motif: NA

Important sites: ACT_SITE 165-165 BINDING 64-64 BINDING 66-66 BINDING 84-84 BINDING 100-100 BINDING 125-125

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQKPKRTKSGSAWMHEHVTDAYVKKAQQDGFRSRAAYKLLEIDSRDHLLHPGMTVVDLG
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHEEECCCCCEECCCCEEEECC
AAPGSWCQVAVQKMKRQGRVLAIDLLPVAPLPGVEALQGDFTAPDTLAWLENTLQAARVD
CCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHH
LVLSDMAPNMSGVMLRDQARHYELCELALDFAVNWLKPDGAFLVKVFQGSGFEDFRNAMR
HHHHCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHH
RAFDQVVIRKPDASRDRSSEVYLLGRRPVKLESVAATGAS
HHHHHHEEECCCCCCCCCCCEEEECCCCCEEHHHHCCCCC
>Mature Secondary Structure 
AQKPKRTKSGSAWMHEHVTDAYVKKAQQDGFRSRAAYKLLEIDSRDHLLHPGMTVVDLG
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHEEECCCCCEECCCCEEEECC
AAPGSWCQVAVQKMKRQGRVLAIDLLPVAPLPGVEALQGDFTAPDTLAWLENTLQAARVD
CCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHH
LVLSDMAPNMSGVMLRDQARHYELCELALDFAVNWLKPDGAFLVKVFQGSGFEDFRNAMR
HHHHCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHH
RAFDQVVIRKPDASRDRSSEVYLLGRRPVKLESVAATGAS
HHHHHHEEECCCCCCCCCCCEEEECCCCCEEHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA