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The map label for this gene is greA [H]

Identifier: 74317147

GI number: 74317147

Start: 1185577

End: 1186053

Strand: Direct

Name: greA [H]

Synonym: Tbd_1129

Alternate gene names: 74317147

Gene position: 1185577-1186053 (Clockwise)

Preceding gene: 74317146

Following gene: 74317148

Centisome position: 40.74

GC content: 64.99

Gene sequence:

>477_bases
GTGACTAAATTTCCCCTCACCGTCGTCGGCGCCGAAAAGCTGCGCGCCGAACTCCAACATCTGAAGAGCGTCCAGCGGCC
GGCTGTGATCCAGGCGATCGCCGAAGCGCGCGCGCAGGGGGACCTTTCCGAAAACGCGGAGTACGACGCTGCCAAGGAGA
AGCAGGGCTTCATCGAAGGCCGCATCGCCGAGCTGGAAGGAAAGCTGTCCAACGCGCAGATCATCGACCCCACGACGCTC
GACGCCGACGGCCGCATCGTCTTCGGCGCGACGGTCGACCTCGAGGACGTCGAGTCCGGCGACAGCGTGACCTACCAGAT
CGTCGGCGACGACGAAGCCGACATCAAGCAGGGCAAGATCTCGGTGTCGTCGCCGATCGCGCGCGCGCTGATCGGGAAGT
ACGCGGGGGACGCCGTCGACGTGCAGGCGCCCGGCGGGGTTCGCCAGTACGAAGTGCTCGACGTTCTTTACAAGTAA

Upstream 100 bases:

>100_bases
CGCGCGCGGCCTGCATGGGCATGCAGCACGGCAACGAACTCGAGGTGTATTCTTTGCAGGCGCTGCACCGGCGTCTGAAC
TGACTGGACAAAGGTAGGCT

Downstream 100 bases:

>100_bases
GCGGTCAGATGCGGCGTTTCTGGGACGGGCTGGCGGGCACCTTGCTCGTGCTGTGGATCGGCGGGCTCTGGGCGATCGGC
TATGTCGCGGCGCCCACCCT

Product: GreA/GreB family elongation factor

Products: NA

Alternate protein names: Transcript cleavage factor greA [H]

Number of amino acids: Translated: 158; Mature: 157

Protein sequence:

>158_residues
MTKFPLTVVGAEKLRAELQHLKSVQRPAVIQAIAEARAQGDLSENAEYDAAKEKQGFIEGRIAELEGKLSNAQIIDPTTL
DADGRIVFGATVDLEDVESGDSVTYQIVGDDEADIKQGKISVSSPIARALIGKYAGDAVDVQAPGGVRQYEVLDVLYK

Sequences:

>Translated_158_residues
MTKFPLTVVGAEKLRAELQHLKSVQRPAVIQAIAEARAQGDLSENAEYDAAKEKQGFIEGRIAELEGKLSNAQIIDPTTL
DADGRIVFGATVDLEDVESGDSVTYQIVGDDEADIKQGKISVSSPIARALIGKYAGDAVDVQAPGGVRQYEVLDVLYK
>Mature_157_residues
TKFPLTVVGAEKLRAELQHLKSVQRPAVIQAIAEARAQGDLSENAEYDAAKEKQGFIEGRIAELEGKLSNAQIIDPTTLD
ADGRIVFGATVDLEDVESGDSVTYQIVGDDEADIKQGKISVSSPIARALIGKYAGDAVDVQAPGGVRQYEVLDVLYK

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082225, Length=157, Percent_Identity=59.2356687898089, Blast_Score=187, Evalue=3e-49,
Organism=Escherichia coli, GI87082261, Length=145, Percent_Identity=38.6206896551724, Blast_Score=91, Evalue=5e-20,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 16930; Mature: 16799

Theoretical pI: Translated: 4.31; Mature: 4.31

Prosite motif: PS00829 GREAB_1 ; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKFPLTVVGAEKLRAELQHLKSVQRPAVIQAIAEARAQGDLSENAEYDAAKEKQGFIEG
CCCCCEEEECHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCHHC
RIAELEGKLSNAQIIDPTTLDADGRIVFGATVDLEDVESGDSVTYQIVGDDEADIKQGKI
CHHHHCCCCCCCEEECCCEECCCCCEEEEEEECHHHCCCCCCEEEEEECCCCCCCCCCEE
SVSSPIARALIGKYAGDAVDVQAPGGVRQYEVLDVLYK
CCCHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHCC
>Mature Secondary Structure 
TKFPLTVVGAEKLRAELQHLKSVQRPAVIQAIAEARAQGDLSENAEYDAAKEKQGFIEG
CCCCEEEECHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCHHC
RIAELEGKLSNAQIIDPTTLDADGRIVFGATVDLEDVESGDSVTYQIVGDDEADIKQGKI
CHHHHCCCCCCCEEECCCEECCCCCEEEEEEECHHHCCCCCCEEEEEECCCCCCCCCCEE
SVSSPIARALIGKYAGDAVDVQAPGGVRQYEVLDVLYK
CCCHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA