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The map label for this gene is carA [H]

Identifier: 74317145

GI number: 74317145

Start: 1181211

End: 1182350

Strand: Direct

Name: carA [H]

Synonym: Tbd_1127

Alternate gene names: 74317145

Gene position: 1181211-1182350 (Clockwise)

Preceding gene: 74317144

Following gene: 74317146

Centisome position: 40.59

GC content: 66.67

Gene sequence:

>1140_bases
TTGTCACGTGCCCAGCCCGCCATCCTCGTTCTCGCCGACGGTTCGGTATTTCGTGGCCGGTCGATCGGCGCCGACGGCAT
CACGACCGGCGAAGTCGTGTTCAATACCGCTCTCACCGGGTACCAGGAAATTCTGACCGATCCGTCGTACTCGCGGCAGA
TCGTGACGCTGACCTATCCGCACATCGGCAACACCGGCGTCAATCCCGAAGACGTCGAGGCGGGGCGCATCCATGCCGCG
GGCCTCGTGGTGCGCGACGTTCCGCGTCGCCACTCGAATTTCCGCTCTGCCGAGTCGCTGAGCGACTATCTCGCGAGCCA
GAACATCGTCGGTATCGCCGACATCGACACCCGACGCCTGACCCGCATCCTGCGCGAGAAGGGCGCGCAGAGCGGCTGCG
TGATGGCCGGCGGGGTCGACGAGAGCAAGGCGCTCGAGGCGGCGCGCGCATTCCCGGGGCTCGCCGGGATGGATCTCGCC
AAGGTGGTCACCGCGCCCGCGATGTACGAATGGCACGAAACCGAATGGCGGCTCGGCCGCGGTTACGGCCAGGTGAGCGA
GCCGCGTTACCACGTCGTGGGTTTCGACTACGGGGTCAAGCGCAACATCCTGCGCATGCTCGCCGAGCGCGGTTGCCGCG
TCACGGTTCTGCCCGCTACCGCCACGGCGGCCGAAGCGCTCGCGCTGAAGCCTGACGGGATTTTCCTGTCCAACGGCCCC
GGCGACCCCGAGCCCTGCGACTACGCGATCCGGGCGATCGGCGAACTGGTCGGCGCAGGCGTACCGACCTTTGGGATTTG
CCTCGGCCATCAACTGCTCGCGCTCGCCTCCGGCGCGAAGACCGTGAAGATGAAATTTGGCCATCACGGCGCGAACCACC
CAGTCAAGGATCTCGACAGCGGGCGCGTCACGATCACGAGCCAGAACCACGGCTTTGCGGTGGACCCCGCGACGCTTCCG
GCCAATCTGCGCAGCACGCATGTCTCGTTGTTTGACGGCTCGCTGCAGGGCCTCGCGCGCACCGACGCGCCCGCGTTCAG
CTTTCAGGGCCACCCCGAAGCCGCCCCCGGTCCCCACGACCTGAGCTACCTGTTCGATCGATTTATCCAGCTGATGGCCG
ACCGTGCCGCAGTACACTGA

Upstream 100 bases:

>100_bases
TCGGCTACAATCTCGTGATTCAAGTTCAGGCGGGTTCGCGCAAGCGGCTCGCCTGAATTTTGTCACCTGCACCACGCCCA
TTCGTCTTGCGGAGTTTTCC

Downstream 100 bases:

>100_bases
TTATGCCCAGCCGCACTGATTTACAAAGCATCCTCATCATCGGCGCGGGCCCGATCGTCATCGGGCAGGCCTGCGAGTTC
GACTACTCCGGCGCGCAGGC

Product: carbamoyl-phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 379; Mature: 378

Protein sequence:

>379_residues
MSRAQPAILVLADGSVFRGRSIGADGITTGEVVFNTALTGYQEILTDPSYSRQIVTLTYPHIGNTGVNPEDVEAGRIHAA
GLVVRDVPRRHSNFRSAESLSDYLASQNIVGIADIDTRRLTRILREKGAQSGCVMAGGVDESKALEAARAFPGLAGMDLA
KVVTAPAMYEWHETEWRLGRGYGQVSEPRYHVVGFDYGVKRNILRMLAERGCRVTVLPATATAAEALALKPDGIFLSNGP
GDPEPCDYAIRAIGELVGAGVPTFGICLGHQLLALASGAKTVKMKFGHHGANHPVKDLDSGRVTITSQNHGFAVDPATLP
ANLRSTHVSLFDGSLQGLARTDAPAFSFQGHPEAAPGPHDLSYLFDRFIQLMADRAAVH

Sequences:

>Translated_379_residues
MSRAQPAILVLADGSVFRGRSIGADGITTGEVVFNTALTGYQEILTDPSYSRQIVTLTYPHIGNTGVNPEDVEAGRIHAA
GLVVRDVPRRHSNFRSAESLSDYLASQNIVGIADIDTRRLTRILREKGAQSGCVMAGGVDESKALEAARAFPGLAGMDLA
KVVTAPAMYEWHETEWRLGRGYGQVSEPRYHVVGFDYGVKRNILRMLAERGCRVTVLPATATAAEALALKPDGIFLSNGP
GDPEPCDYAIRAIGELVGAGVPTFGICLGHQLLALASGAKTVKMKFGHHGANHPVKDLDSGRVTITSQNHGFAVDPATLP
ANLRSTHVSLFDGSLQGLARTDAPAFSFQGHPEAAPGPHDLSYLFDRFIQLMADRAAVH
>Mature_378_residues
SRAQPAILVLADGSVFRGRSIGADGITTGEVVFNTALTGYQEILTDPSYSRQIVTLTYPHIGNTGVNPEDVEAGRIHAAG
LVVRDVPRRHSNFRSAESLSDYLASQNIVGIADIDTRRLTRILREKGAQSGCVMAGGVDESKALEAARAFPGLAGMDLAK
VVTAPAMYEWHETEWRLGRGYGQVSEPRYHVVGFDYGVKRNILRMLAERGCRVTVLPATATAAEALALKPDGIFLSNGPG
DPEPCDYAIRAIGELVGAGVPTFGICLGHQLLALASGAKTVKMKFGHHGANHPVKDLDSGRVTITSQNHGFAVDPATLPA
NLRSTHVSLFDGSLQGLARTDAPAFSFQGHPEAAPGPHDLSYLFDRFIQLMADRAAVH

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=383, Percent_Identity=39.686684073107, Blast_Score=247, Evalue=1e-65,
Organism=Homo sapiens, GI21361331, Length=384, Percent_Identity=34.6354166666667, Blast_Score=206, Evalue=2e-53,
Organism=Homo sapiens, GI169790915, Length=384, Percent_Identity=34.6354166666667, Blast_Score=206, Evalue=3e-53,
Organism=Escherichia coli, GI1786215, Length=372, Percent_Identity=66.3978494623656, Blast_Score=516, Evalue=1e-147,
Organism=Caenorhabditis elegans, GI193204318, Length=378, Percent_Identity=38.8888888888889, Blast_Score=239, Evalue=2e-63,
Organism=Saccharomyces cerevisiae, GI6324878, Length=383, Percent_Identity=39.1644908616188, Blast_Score=243, Evalue=3e-65,
Organism=Saccharomyces cerevisiae, GI6322331, Length=390, Percent_Identity=37.6923076923077, Blast_Score=236, Evalue=4e-63,
Organism=Drosophila melanogaster, GI45555749, Length=386, Percent_Identity=38.3419689119171, Blast_Score=234, Evalue=1e-61,
Organism=Drosophila melanogaster, GI24642586, Length=386, Percent_Identity=38.0829015544041, Blast_Score=233, Evalue=1e-61,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 40472; Mature: 40341

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRAQPAILVLADGSVFRGRSIGADGITTGEVVFNTALTGYQEILTDPSYSRQIVTLTYP
CCCCCCEEEEEECCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECC
HIGNTGVNPEDVEAGRIHAAGLVVRDVPRRHSNFRSAESLSDYLASQNIVGIADIDTRRL
CCCCCCCCCCCCCCCCEEECCCHHHHCCHHHCCCCCHHHHHHHHHCCCCEEEECCCHHHH
TRILREKGAQSGCVMAGGVDESKALEAARAFPGLAGMDLAKVVTAPAMYEWHETEWRLGR
HHHHHHCCCCCCEEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHCCHHEECCCCCHHCCC
GYGQVSEPRYHVVGFDYGVKRNILRMLAERGCRVTVLPATATAAEALALKPDGIFLSNGP
CCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCEEEEEECCHHHHHHEEECCCCEEEECCC
GDPEPCDYAIRAIGELVGAGVPTFGICLGHQLLALASGAKTVKMKFGHHGANHPVKDLDS
CCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCC
GRVTITSQNHGFAVDPATLPANLRSTHVSLFDGSLQGLARTDAPAFSFQGHPEAAPGPHD
CEEEEEECCCCEEECCCCCCCCCHHCEEEEECCCHHHHHHCCCCCCCCCCCCCCCCCCCH
LSYLFDRFIQLMADRAAVH
HHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SRAQPAILVLADGSVFRGRSIGADGITTGEVVFNTALTGYQEILTDPSYSRQIVTLTYP
CCCCCEEEEEECCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECC
HIGNTGVNPEDVEAGRIHAAGLVVRDVPRRHSNFRSAESLSDYLASQNIVGIADIDTRRL
CCCCCCCCCCCCCCCCEEECCCHHHHCCHHHCCCCCHHHHHHHHHCCCCEEEECCCHHHH
TRILREKGAQSGCVMAGGVDESKALEAARAFPGLAGMDLAKVVTAPAMYEWHETEWRLGR
HHHHHHCCCCCCEEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHCCHHEECCCCCHHCCC
GYGQVSEPRYHVVGFDYGVKRNILRMLAERGCRVTVLPATATAAEALALKPDGIFLSNGP
CCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCEEEEEECCHHHHHHEEECCCCEEEECCC
GDPEPCDYAIRAIGELVGAGVPTFGICLGHQLLALASGAKTVKMKFGHHGANHPVKDLDS
CCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCC
GRVTITSQNHGFAVDPATLPANLRSTHVSLFDGSLQGLARTDAPAFSFQGHPEAAPGPHD
CEEEEEECCCCEEECCCCCCCCCHHCEEEEECCCHHHHHHCCCCCCCCCCCCCCCCCCCH
LSYLFDRFIQLMADRAAVH
HHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]