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The map label for this gene is cls1 [H]

Identifier: 74317069

GI number: 74317069

Start: 1115528

End: 1116787

Strand: Reverse

Name: cls1 [H]

Synonym: Tbd_1051

Alternate gene names: 74317069

Gene position: 1116787-1115528 (Counterclockwise)

Preceding gene: 74317072

Following gene: 74317068

Centisome position: 38.38

GC content: 63.25

Gene sequence:

>1260_bases
ATGCTCGTTTTCTGGATCGTCCTCGGCGCGGTGCTGGCCACCGTCATCGCGCTGAACTTTCTGCCGGGGGAAAAGAAGGT
CCAGACCCGGATCAGCGAGCTCTATTTCATCCACGACCCGCAGTTCCTGCGCTCGATGAGCCATCTGCTCGGCCCGCCAC
TCGTCCCGGGCAACCGCGCCCAGGAGCTCATCAACGGCGACGAGATCTTTCCACCGATGCTGGCGGCGATTCGCGCGGCG
CGCAAAACGATCACCTTCGAGACCTACATCTACTGGTCTGGCGAAATCGGCAAGGAATTTGCCGAGGCGCTCGCCGAACG
CGCACGCGCCGGGGTCAAGGTCCATGTCCTCGTCGACTGGGCGGGCAGCGTCAAGATGGAAGACGACTTGCTCGAGCTGA
TGAAAGACGGCGGAGTCGAGATCCTCAAATATCACCCGCTGCACTGGTACCACCTCGCGCGCATGAACAACCGCACGCAC
CGCAAACTCCTGGTCGTCGACGGGCACATCGGCTTCACGGGCGGCGTCGGCATCGCCGACACCTGGCTCGGCCATGCGCA
GGATGACAATCACTGGCGGGACTCGCATTTCCGCGTCGAAGGTCCCGTGGTCGCCCACATGCAGGCGGCCTTCCTCGACA
ACTGGATGAAAACCAGCGGGGCGGTGTTGCACGGTGAGGACTATTTTCCGGCGCTCGAGCCCGTCGGCGAGCACTACGCT
CAGGTCTTCAAGAGCTCCTCGTCGGAAGGCAGCGAAAGCGTGCGCCTGATGTATCTGCTGTCGATCGCGGCGGCGCGCGA
AACGGTCTATATCGCCAATGCTTATTTCGTGCCTGACGACCTTTCGGTAGAGACCCTCACCCGCGCGCTGCGCCGGGACG
TGAAAGTCAGGATCCTCGTGCCCGGCAGGCACACCGACTCGGGGCTCGCGCGCCGCGCGTCGCGCGCCCGCTGGGGCGCG
TTGCTCGTGGCGGGGGCCGAGATATACGAATATCAGCCTACGATGTTCCACTGCAAGCTCATGATCGTCGACGACGTCTG
GGTGTCGGTCGGCTCGACCAACTTCGATTCGCGATCGTTCCGCCTCAACGACGAAGCCAATCTCAACATCTACGACCACG
CCTTCGCAGCGCGCCAGATCGCGATCTTCGAGGACGACCTCAGAAAAGCCCGGCGGGTCGACTACGATGCGTGGAAGCGC
CGCCCCTGGCGCGAGAAGCTGCTCGAACACGGCTCCGCATTGTTCCGCTCGCAAGTCTGA

Upstream 100 bases:

>100_bases
ATTAGGATGGGCCAGCGGTGCGCGATAGCGGGTGGCGCCGCCCGGCCCCATCCCTTTGCGCGATGGGCATCTCAACCTCA
GGGCGTCTTCAAGAACAAAA

Downstream 100 bases:

>100_bases
CCAAGGAGACGCAAGACATCATGACGCGCCCGACTGTCTACCACATCCCGGTATGCCCGTTCTGTCAGCGCGTCGAGATC
CTGCTGTCGCTCAAGGGCCG

Product: phospholipase D/transphosphatidylase

Products: Biphosphatidylglycerol; Glycerol. [C]

Alternate protein names: CL synthase 1 [H]

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MLVFWIVLGAVLATVIALNFLPGEKKVQTRISELYFIHDPQFLRSMSHLLGPPLVPGNRAQELINGDEIFPPMLAAIRAA
RKTITFETYIYWSGEIGKEFAEALAERARAGVKVHVLVDWAGSVKMEDDLLELMKDGGVEILKYHPLHWYHLARMNNRTH
RKLLVVDGHIGFTGGVGIADTWLGHAQDDNHWRDSHFRVEGPVVAHMQAAFLDNWMKTSGAVLHGEDYFPALEPVGEHYA
QVFKSSSSEGSESVRLMYLLSIAAARETVYIANAYFVPDDLSVETLTRALRRDVKVRILVPGRHTDSGLARRASRARWGA
LLVAGAEIYEYQPTMFHCKLMIVDDVWVSVGSTNFDSRSFRLNDEANLNIYDHAFAARQIAIFEDDLRKARRVDYDAWKR
RPWREKLLEHGSALFRSQV

Sequences:

>Translated_419_residues
MLVFWIVLGAVLATVIALNFLPGEKKVQTRISELYFIHDPQFLRSMSHLLGPPLVPGNRAQELINGDEIFPPMLAAIRAA
RKTITFETYIYWSGEIGKEFAEALAERARAGVKVHVLVDWAGSVKMEDDLLELMKDGGVEILKYHPLHWYHLARMNNRTH
RKLLVVDGHIGFTGGVGIADTWLGHAQDDNHWRDSHFRVEGPVVAHMQAAFLDNWMKTSGAVLHGEDYFPALEPVGEHYA
QVFKSSSSEGSESVRLMYLLSIAAARETVYIANAYFVPDDLSVETLTRALRRDVKVRILVPGRHTDSGLARRASRARWGA
LLVAGAEIYEYQPTMFHCKLMIVDDVWVSVGSTNFDSRSFRLNDEANLNIYDHAFAARQIAIFEDDLRKARRVDYDAWKR
RPWREKLLEHGSALFRSQV
>Mature_419_residues
MLVFWIVLGAVLATVIALNFLPGEKKVQTRISELYFIHDPQFLRSMSHLLGPPLVPGNRAQELINGDEIFPPMLAAIRAA
RKTITFETYIYWSGEIGKEFAEALAERARAGVKVHVLVDWAGSVKMEDDLLELMKDGGVEILKYHPLHWYHLARMNNRTH
RKLLVVDGHIGFTGGVGIADTWLGHAQDDNHWRDSHFRVEGPVVAHMQAAFLDNWMKTSGAVLHGEDYFPALEPVGEHYA
QVFKSSSSEGSESVRLMYLLSIAAARETVYIANAYFVPDDLSVETLTRALRRDVKVRILVPGRHTDSGLARRASRARWGA
LLVAGAEIYEYQPTMFHCKLMIVDDVWVSVGSTNFDSRSFRLNDEANLNIYDHAFAARQIAIFEDDLRKARRVDYDAWKR
RPWREKLLEHGSALFRSQV

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=363, Percent_Identity=30.0275482093664, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI1787007, Length=323, Percent_Identity=32.1981424148607, Blast_Score=154, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.- [C]

Molecular weight: Translated: 47581; Mature: 47581

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVFWIVLGAVLATVIALNFLPGEKKVQTRISELYFIHDPQFLRSMSHLLGPPLVPGNRA
CEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHEEEEECHHHHHHHHHHCCCCCCCCCHH
QELINGDEIFPPMLAAIRAARKTITFETYIYWSGEIGKEFAEALAERARAGVKVHVLVDW
HHHCCCCCCCHHHHHHHHHHHHEEEEEEEEEECCHHHHHHHHHHHHHHHCCCEEEEEEEE
AGSVKMEDDLLELMKDGGVEILKYHPLHWYHLARMNNRTHRKLLVVDGHIGFTGGVGIAD
CCCCEEHHHHHHHHHCCCEEEEEECCCCEEEEHHCCCCCCEEEEEEECCCCCCCCCCHHH
TWLGHAQDDNHWRDSHFRVEGPVVAHMQAAFLDNWMKTSGAVLHGEDYFPALEPVGEHYA
HHCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHH
QVFKSSSSEGSESVRLMYLLSIAAARETVYIANAYFVPDDLSVETLTRALRRDVKVRILV
HHHHCCCCCCCHHHHHHHHHHHHHHCCEEEEEEEEECCCCCCHHHHHHHHHCCCEEEEEE
PGRHTDSGLARRASRARWGALLVAGAEIYEYQPTMFHCKLMIVDDVWVSVGSTNFDSRSF
CCCCCCHHHHHHHHHHHHCEEEEECCHHEECCCCEEEEEEEEEEEEEHCCCCCCCCCCEE
RLNDEANLNIYDHAFAARQIAIFEDDLRKARRVDYDAWKRRPWREKLLEHGSALFRSQV
EECCCCCCEEEEHHHHHHEEEEEHHHHHHHHHCCHHHHHCCCHHHHHHHCCHHHHHCCC
>Mature Secondary Structure
MLVFWIVLGAVLATVIALNFLPGEKKVQTRISELYFIHDPQFLRSMSHLLGPPLVPGNRA
CEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHEEEEECHHHHHHHHHHCCCCCCCCCHH
QELINGDEIFPPMLAAIRAARKTITFETYIYWSGEIGKEFAEALAERARAGVKVHVLVDW
HHHCCCCCCCHHHHHHHHHHHHEEEEEEEEEECCHHHHHHHHHHHHHHHCCCEEEEEEEE
AGSVKMEDDLLELMKDGGVEILKYHPLHWYHLARMNNRTHRKLLVVDGHIGFTGGVGIAD
CCCCEEHHHHHHHHHCCCEEEEEECCCCEEEEHHCCCCCCEEEEEEECCCCCCCCCCHHH
TWLGHAQDDNHWRDSHFRVEGPVVAHMQAAFLDNWMKTSGAVLHGEDYFPALEPVGEHYA
HHCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHH
QVFKSSSSEGSESVRLMYLLSIAAARETVYIANAYFVPDDLSVETLTRALRRDVKVRILV
HHHHCCCCCCCHHHHHHHHHHHHHHCCEEEEEEEEECCCCCCHHHHHHHHHCCCEEEEEE
PGRHTDSGLARRASRARWGALLVAGAEIYEYQPTMFHCKLMIVDDVWVSVGSTNFDSRSF
CCCCCCHHHHHHHHHHHHCEEEEECCHHEECCCCEEEEEEEEEEEEEHCCCCCCCCCCEE
RLNDEANLNIYDHAFAARQIAIFEDDLRKARRVDYDAWKRRPWREKLLEHGSALFRSQV
EECCCCCCEEEEHHHHHHEEEEEHHHHHHHHHCCHHHHHCCCHHHHHHHCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Phosphatidylglycerol [C]

Specific reaction: 2 Phosphatidylglycerol = Biphosphatidylglycerol + Glycerol. [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]