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The map label for this gene is pyrF

Identifier: 74316978

GI number: 74316978

Start: 1022197

End: 1022934

Strand: Direct

Name: pyrF

Synonym: Tbd_0960

Alternate gene names: 74316978

Gene position: 1022197-1022934 (Clockwise)

Preceding gene: 74316977

Following gene: 74316979

Centisome position: 35.13

GC content: 71.0

Gene sequence:

>738_bases
ATGACGCACAAGATTATCGTCGCGCTGGATTTTCCGGCGGGTGCCCCGGCGCTGGCACTGGCGGATCGGCTCGATCCCGC
ACGATGCCGGCTCAAGGTCGGCAAGGAGTTGTTCACGGCAGCCGGACCCGATCTCGTGCGCGCGCTGGTCGCGCGCGGCT
TCGAGGTCTTTCTCGATCTCAAGTTTCACGATATCCCGAATACCGTCGCCGCCGCGTGCCGCGCGGCCGCGGGACTCGGC
GTGTGGATGCTCAACGTCCACGCCGCGGGCGGTCGGCGCATGATGGACGCCGCCCGCGAGGCGCTCGAATCCCTGCCCCC
GGGGGATGGCGCCGCGGATACGCCGCAACGCCCTTTGCTGATCGCGGTCACTGTGTTGACGAGCATGAGCGCCGGCGACC
TCGCCGAGACCGGCGTCGCCGACACGCCGGCCGAGCAGGTGATGCGGCTCGCCCGCCTCGCACACGCCTGCGCGCTCGAC
GGCGTCGTCTGCTCCGCGCAGGAGGCCGCAGGTCTGCGTGCCGCGCTCGGCGCGGACTTCCGTCTGGTCACCCCGGGGAT
TCGCCCGGCGGGCGCCGCAGCGGCGGACCAGCGCCGCGTGATGACGCCGGTCGAGGCGCTGCGCGCCGGAGCCACCGATC
TGGTGATCGGCCGCCCCATCACCGGCGCGGGCGATCCGCTCGCGGCGCTCGCGGCGATTCAAGCGGACATTGAAGAAAAT
CTAGGGAGAGCGTTTTGA

Upstream 100 bases:

>100_bases
TCTTCTGGCGTTGTCCGGCGTGCGGGCGCTGGGACAGCTTCGATCCCGAGCGCGGCGAAGGCGAGAGCTAGAGGCACGAT
CCAGGGGGCAGGGAAGAAGA

Downstream 100 bases:

>100_bases
AAATCACTGTGATCGGCACGGGCTACGTCGGCCTGGTGACCGGAACCTGCCTGGCCGAGGTCGGCAACGACGTGCTGTGC
CTCGATCTCGATCCGAAGAA

Product: orotidine-5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase

Number of amino acids: Translated: 245; Mature: 244

Protein sequence:

>245_residues
MTHKIIVALDFPAGAPALALADRLDPARCRLKVGKELFTAAGPDLVRALVARGFEVFLDLKFHDIPNTVAAACRAAAGLG
VWMLNVHAAGGRRMMDAAREALESLPPGDGAADTPQRPLLIAVTVLTSMSAGDLAETGVADTPAEQVMRLARLAHACALD
GVVCSAQEAAGLRAALGADFRLVTPGIRPAGAAAADQRRVMTPVEALRAGATDLVIGRPITGAGDPLAALAAIQADIEEN
LGRAF

Sequences:

>Translated_245_residues
MTHKIIVALDFPAGAPALALADRLDPARCRLKVGKELFTAAGPDLVRALVARGFEVFLDLKFHDIPNTVAAACRAAAGLG
VWMLNVHAAGGRRMMDAAREALESLPPGDGAADTPQRPLLIAVTVLTSMSAGDLAETGVADTPAEQVMRLARLAHACALD
GVVCSAQEAAGLRAALGADFRLVTPGIRPAGAAAADQRRVMTPVEALRAGATDLVIGRPITGAGDPLAALAAIQADIEEN
LGRAF
>Mature_244_residues
THKIIVALDFPAGAPALALADRLDPARCRLKVGKELFTAAGPDLVRALVARGFEVFLDLKFHDIPNTVAAACRAAAGLGV
WMLNVHAAGGRRMMDAAREALESLPPGDGAADTPQRPLLIAVTVLTSMSAGDLAETGVADTPAEQVMRLARLAHACALDG
VVCSAQEAAGLRAALGADFRLVTPGIRPAGAAAADQRRVMTPVEALRAGATDLVIGRPITGAGDPLAALAAIQADIEENL
GRAF

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP)

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1787537, Length=236, Percent_Identity=58.0508474576271, Blast_Score=260, Evalue=7e-71,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PYRF_THIDA (Q3SK77)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_314718.1
- ProteinModelPortal:   Q3SK77
- SMR:   Q3SK77
- STRING:   Q3SK77
- GeneID:   3672752
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_0960
- NMPDR:   fig|292415.3.peg.588
- eggNOG:   COG0284
- HOGENOM:   HBG625253
- OMA:   TVHAYPQ
- PhylomeDB:   Q3SK77
- BioCyc:   TDEN292415:TBD_0960-MONOMER
- HAMAP:   MF_01200_B
- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00934
- TIGRFAMs:   TIGR01740

Pfam domain/function: PF00215 OMPdecase; SSF51366 RibP_bind_barrel

EC number: =4.1.1.23

Molecular weight: Translated: 25177; Mature: 25046

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS00156 OMPDECASE

Important sites: ACT_SITE 61-61 BINDING 10-10 BINDING 32-32 BINDING 127-127 BINDING 188-188 BINDING 197-197 BINDING 217-217 BINDING 218-218

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHKIIVALDFPAGAPALALADRLDPARCRLKVGKELFTAAGPDLVRALVARGFEVFLDL
CCCEEEEEEECCCCCCHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEEEE
KFHDIPNTVAAACRAAAGLGVWMLNVHAAGGRRMMDAAREALESLPPGDGAADTPQRPLL
EECCCCHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCE
IAVTVLTSMSAGDLAETGVADTPAEQVMRLARLAHACALDGVVCSAQEAAGLRAALGADF
EHHHHHHCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHCCCE
RLVTPGIRPAGAAAADQRRVMTPVEALRAGATDLVIGRPITGAGDPLAALAAIQADIEEN
EEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHH
LGRAF
CCCCC
>Mature Secondary Structure 
THKIIVALDFPAGAPALALADRLDPARCRLKVGKELFTAAGPDLVRALVARGFEVFLDL
CCEEEEEEECCCCCCHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEEEE
KFHDIPNTVAAACRAAAGLGVWMLNVHAAGGRRMMDAAREALESLPPGDGAADTPQRPLL
EECCCCHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCE
IAVTVLTSMSAGDLAETGVADTPAEQVMRLARLAHACALDGVVCSAQEAAGLRAALGADF
EHHHHHHCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHCCCE
RLVTPGIRPAGAAAADQRRVMTPVEALRAGATDLVIGRPITGAGDPLAALAAIQADIEEN
EEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHH
LGRAF
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA