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The map label for this gene is ispB [H]

Identifier: 74316881

GI number: 74316881

Start: 913981

End: 915012

Strand: Reverse

Name: ispB [H]

Synonym: Tbd_0863

Alternate gene names: 74316881

Gene position: 915012-913981 (Counterclockwise)

Preceding gene: 74316885

Following gene: 74316880

Centisome position: 31.45

GC content: 61.72

Gene sequence:

>1032_bases
ATGAAAAACGGCGCCCACACTGCTACCATTGCTGATTTCGCAACCGCTCCCCCATCCCCTATCGTGTCCCTGGAAAGCCT
GCAATCGTTTCTGGCCGATGATATGCGCGCCGTCGATCATGTCATCCGCGAGCGCCTGTATTCCGACGTGGTGCTGATCC
GCCAGGTCTCGGAATACATCATCAACAGCGGGGGCAAACGCCTGCGTCCGGCGCTCGTGCTGCTGTCGGCCGGTTGCTTC
GGCTACCGCGGACAGGCGCATCACGAACTCGCCGCGGTCGTCGAGTTCATCCACACGGCCACCTTGCTGCACGACGACGT
CGTCGACGAATCTGAACTCCGTCGCGGCCGCGAAACCGCCAACGCGCTGTTCGGCAACGCGGCGAGCGTGCTGGTGGGCG
ACTTCCTCTATTCGCGCGCGTTCCAGATGATGGTCGGCGTCGACAACATGGAAGTCATGCGCATCCTGTCCGATGCGACC
AATACCATCGCCGAAGGCGAGGTCCTGCAGCTGCTCAACTGCCACGACCCCGACATCGACGAAGAAAACTATCTGCGCGT
GATCCGCTACAAGACCGCGAAGCTGTTCGAGGCCGCGGGACGCCTCGGCGCTGTCATCGGCGGCGGCAGCGAAGCCGACA
AGGACGCACTGGCGCGCTACGGCATGCACCTCGGCACTGCCTTCCAGTTGATCGACGACGTGCTCGACTACTCGGGCGAC
TTCCTCAAGACCGGAAAGAACATCGGCGACGACCTCGCCGAAGGCAAGCCCACGCTGCCGCTCCTCTATGCCATGAAGCA
CGGCAGCGCGGCGCAGGCCGCGAGCATTCGTCACGCGATCGAACACGGCGGCCTGACCGAATTCCAGAGCGTGCTCGCCG
CGATCAAGGATACCAACGCGCTGCAATACACGCGTGAAGTCGCACAGCGCGAAGTGCAGACGGCCAATGCAGCAATTTCT
CGTCTGCCGGATTCAAATTCCAAAACAGCTTTGCTACAATTGGCGGCTTTCGCGGTCGAGCGAAGTTTTTGA

Upstream 100 bases:

>100_bases
AATACGAACCCAGGAAAACGCGAGATTCTAGCGGCTCCCCTCCGCCGAGTCAAACGCTTTCGGGCGCTCGGCACGATGCG
TCAATCGGTCGCTCGCGCAA

Downstream 100 bases:

>100_bases
CCACGAAATCTGGTGTTTCCCGGTAGTTACAAAATCGGTTCAGTACCCACTATTGACCGTCCATCTGCCTCGGGGTGTAG
CTCAGCCTGGTAGAGTACTG

Product: trans-hexaprenyltranstransferase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MKNGAHTATIADFATAPPSPIVSLESLQSFLADDMRAVDHVIRERLYSDVVLIRQVSEYIINSGGKRLRPALVLLSAGCF
GYRGQAHHELAAVVEFIHTATLLHDDVVDESELRRGRETANALFGNAASVLVGDFLYSRAFQMMVGVDNMEVMRILSDAT
NTIAEGEVLQLLNCHDPDIDEENYLRVIRYKTAKLFEAAGRLGAVIGGGSEADKDALARYGMHLGTAFQLIDDVLDYSGD
FLKTGKNIGDDLAEGKPTLPLLYAMKHGSAAQAASIRHAIEHGGLTEFQSVLAAIKDTNALQYTREVAQREVQTANAAIS
RLPDSNSKTALLQLAAFAVERSF

Sequences:

>Translated_343_residues
MKNGAHTATIADFATAPPSPIVSLESLQSFLADDMRAVDHVIRERLYSDVVLIRQVSEYIINSGGKRLRPALVLLSAGCF
GYRGQAHHELAAVVEFIHTATLLHDDVVDESELRRGRETANALFGNAASVLVGDFLYSRAFQMMVGVDNMEVMRILSDAT
NTIAEGEVLQLLNCHDPDIDEENYLRVIRYKTAKLFEAAGRLGAVIGGGSEADKDALARYGMHLGTAFQLIDDVLDYSGD
FLKTGKNIGDDLAEGKPTLPLLYAMKHGSAAQAASIRHAIEHGGLTEFQSVLAAIKDTNALQYTREVAQREVQTANAAIS
RLPDSNSKTALLQLAAFAVERSF
>Mature_343_residues
MKNGAHTATIADFATAPPSPIVSLESLQSFLADDMRAVDHVIRERLYSDVVLIRQVSEYIINSGGKRLRPALVLLSAGCF
GYRGQAHHELAAVVEFIHTATLLHDDVVDESELRRGRETANALFGNAASVLVGDFLYSRAFQMMVGVDNMEVMRILSDAT
NTIAEGEVLQLLNCHDPDIDEENYLRVIRYKTAKLFEAAGRLGAVIGGGSEADKDALARYGMHLGTAFQLIDDVLDYSGD
FLKTGKNIGDDLAEGKPTLPLLYAMKHGSAAQAASIRHAIEHGGLTEFQSVLAAIKDTNALQYTREVAQREVQTANAAIS
RLPDSNSKTALLQLAAFAVERSF

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=315, Percent_Identity=30.1587301587302, Blast_Score=141, Evalue=8e-34,
Organism=Homo sapiens, GI4758430, Length=253, Percent_Identity=24.1106719367589, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI83700220, Length=253, Percent_Identity=24.1106719367589, Blast_Score=69, Evalue=5e-12,
Organism=Escherichia coli, GI1789578, Length=321, Percent_Identity=54.8286604361371, Blast_Score=354, Evalue=5e-99,
Organism=Escherichia coli, GI1786623, Length=218, Percent_Identity=35.3211009174312, Blast_Score=94, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17505681, Length=320, Percent_Identity=29.0625, Blast_Score=136, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6319475, Length=288, Percent_Identity=32.2916666666667, Blast_Score=129, Evalue=7e-31,
Organism=Saccharomyces cerevisiae, GI6325188, Length=299, Percent_Identity=25.752508361204, Blast_Score=80, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24651612, Length=294, Percent_Identity=27.891156462585, Blast_Score=122, Evalue=2e-28,
Organism=Drosophila melanogaster, GI281365769, Length=281, Percent_Identity=23.4875444839858, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24660002, Length=281, Percent_Identity=23.4875444839858, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 37248; Mature: 37248

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNGAHTATIADFATAPPSPIVSLESLQSFLADDMRAVDHVIRERLYSDVVLIRQVSEYI
CCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
INSGGKRLRPALVLLSAGCFGYRGQAHHELAAVVEFIHTATLLHDDVVDESELRRGRETA
HHCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
NALFGNAASVLVGDFLYSRAFQMMVGVDNMEVMRILSDATNTIAEGEVLQLLNCHDPDID
HHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCC
EENYLRVIRYKTAKLFEAAGRLGAVIGGGSEADKDALARYGMHLGTAFQLIDDVLDYSGD
HHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
FLKTGKNIGDDLAEGKPTLPLLYAMKHGSAAQAASIRHAIEHGGLTEFQSVLAAIKDTNA
HHHCCCCCCHHHHCCCCCCHHHHEECCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHH
LQYTREVAQREVQTANAAISRLPDSNSKTALLQLAAFAVERSF
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKNGAHTATIADFATAPPSPIVSLESLQSFLADDMRAVDHVIRERLYSDVVLIRQVSEYI
CCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
INSGGKRLRPALVLLSAGCFGYRGQAHHELAAVVEFIHTATLLHDDVVDESELRRGRETA
HHCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
NALFGNAASVLVGDFLYSRAFQMMVGVDNMEVMRILSDATNTIAEGEVLQLLNCHDPDID
HHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCC
EENYLRVIRYKTAKLFEAAGRLGAVIGGGSEADKDALARYGMHLGTAFQLIDDVLDYSGD
HHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
FLKTGKNIGDDLAEGKPTLPLLYAMKHGSAAQAASIRHAIEHGGLTEFQSVLAAIKDTNA
HHHCCCCCCHHHHCCCCCCHHHHEECCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHH
LQYTREVAQREVQTANAAISRLPDSNSKTALLQLAAFAVERSF
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]