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The map label for this gene is rpoS [H]

Identifier: 74316856

GI number: 74316856

Start: 888316

End: 889266

Strand: Direct

Name: rpoS [H]

Synonym: Tbd_0838

Alternate gene names: 74316856

Gene position: 888316-889266 (Clockwise)

Preceding gene: 74316855

Following gene: 74316857

Centisome position: 30.53

GC content: 65.19

Gene sequence:

>951_bases
ATGAGCCGCGAACACAGCGATGAATCGGATGACTTGACGCCTGACGAAACCGAGGCCGACGAGGAACGTCACGCCGCCTC
GGACAGCAACGAGGAATTGGCCTCGGCCATCCTCGACGAACCGCAGGTCGACGTCACCCAACTGTATCTCAACGAAATCG
GTCAGGCGCCGCTCTTGACCGCGGCGGAGGAAGTCGCGCTGGCCCGCCGCACCGTGCTTGGCGACTTCGAAGCGCGCCAG
ACCATGATCGAGCGCAACCTGCGCCTGGTCGTCAACATCGCCAAGCACTACGCCAACCGCGGCCTCACCCTGCTCGACCT
GGTCGAGGAGGGCAACCTCGGGCTGATCCACGCACTCGAGAAATTCGATCCCGAGCGCGGCTTCCGCTTCTCGACCTACG
CGACCTGGTGGATCCGCCAGAACATCGAGCGCGCGATCATGAACCAGTCGCGCACGATCCGGCTGCCGGTTCACATCATC
AAGGAACTCAACATCTATCTGCGCGCCCAGCGCCATCTCGAGACCCACGGCGTCACCGACGCGACGTCCGACGACATCGC
CGCGCTGACCGGACACCCGGTCGCCGACGTGCGCCGCATCATGCAGCTGAACGAGCGGATCGCCTCGCTCGACGCGCCGC
TCGACGTCGACCCCACGCTCTCCCTCGGCGAGGCGATCGCGGACGACAACGCGCATCTCCCCGACGAGATGCTGCAACTC
GAGGAGATCGAGAACCACGTCCAGGAATGGCTGACCCAGCTCAACGACAAGCAGCGCTGGGTGATCGAGCGCCGTTTCGG
GCTCAACGGCTGCGAGGCCTGCACGCTCGAAGACCTGGCCCTCGAACTCGAAGTGACGCGCGAACGCGTGCGCCAGATCC
AGATGGAATCGCTGCGTGTGCTGCGGCAGCTGCTGCGGCGCCGCGGCGTGACCAAGGACGAGCTGTTCTAG

Upstream 100 bases:

>100_bases
CCCTCATGGGCGACAGCGACGCCGACCGCGTGAAACTGCATTTCGAGATCCGCCGTTATGGCAAACCGCTTGACCCACTG
AATTATCTGCCGGAGCGTTC

Downstream 100 bases:

>100_bases
GGCGGGCGCCTGCGTCCCGCGCGGCGTCGAGGAATCCGCCATGATCGACTGGTCCCGCATCGGCACCGTGTTTCTCGACA
TGGACGGCACGCTGCTCGAT

Product: RNA polymerase sigma factor RpoS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MSREHSDESDDLTPDETEADEERHAASDSNEELASAILDEPQVDVTQLYLNEIGQAPLLTAAEEVALARRTVLGDFEARQ
TMIERNLRLVVNIAKHYANRGLTLLDLVEEGNLGLIHALEKFDPERGFRFSTYATWWIRQNIERAIMNQSRTIRLPVHII
KELNIYLRAQRHLETHGVTDATSDDIAALTGHPVADVRRIMQLNERIASLDAPLDVDPTLSLGEAIADDNAHLPDEMLQL
EEIENHVQEWLTQLNDKQRWVIERRFGLNGCEACTLEDLALELEVTRERVRQIQMESLRVLRQLLRRRGVTKDELF

Sequences:

>Translated_316_residues
MSREHSDESDDLTPDETEADEERHAASDSNEELASAILDEPQVDVTQLYLNEIGQAPLLTAAEEVALARRTVLGDFEARQ
TMIERNLRLVVNIAKHYANRGLTLLDLVEEGNLGLIHALEKFDPERGFRFSTYATWWIRQNIERAIMNQSRTIRLPVHII
KELNIYLRAQRHLETHGVTDATSDDIAALTGHPVADVRRIMQLNERIASLDAPLDVDPTLSLGEAIADDNAHLPDEMLQL
EEIENHVQEWLTQLNDKQRWVIERRFGLNGCEACTLEDLALELEVTRERVRQIQMESLRVLRQLLRRRGVTKDELF
>Mature_315_residues
SREHSDESDDLTPDETEADEERHAASDSNEELASAILDEPQVDVTQLYLNEIGQAPLLTAAEEVALARRTVLGDFEARQT
MIERNLRLVVNIAKHYANRGLTLLDLVEEGNLGLIHALEKFDPERGFRFSTYATWWIRQNIERAIMNQSRTIRLPVHIIK
ELNIYLRAQRHLETHGVTDATSDDIAALTGHPVADVRRIMQLNERIASLDAPLDVDPTLSLGEAIADDNAHLPDEMLQLE
EIENHVQEWLTQLNDKQRWVIERRFGLNGCEACTLEDLALELEVTRERVRQIQMESLRVLRQLLRRRGVTKDELF

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is required for the normal expression of the enterotoxin, yst, in the stationary phase [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=288, Percent_Identity=56.5972222222222, Blast_Score=320, Evalue=9e-89,
Organism=Escherichia coli, GI1789448, Length=256, Percent_Identity=38.28125, Blast_Score=184, Evalue=9e-48,
Organism=Escherichia coli, GI1789871, Length=274, Percent_Identity=28.8321167883212, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012761
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 36235; Mature: 36104

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSREHSDESDDLTPDETEADEERHAASDSNEELASAILDEPQVDVTQLYLNEIGQAPLLT
CCCCCCCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHH
AAEEVALARRTVLGDFEARQTMIERNLRLVVNIAKHYANRGLTLLDLVEEGNLGLIHALE
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHCCCCHHHHHHH
KFDPERGFRFSTYATWWIRQNIERAIMNQSRTIRLPVHIIKELNIYLRAQRHLETHGVTD
HCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCC
ATSDDIAALTGHPVADVRRIMQLNERIASLDAPLDVDPTLSLGEAIADDNAHLPDEMLQL
CCCCCCHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHH
EEIENHVQEWLTQLNDKQRWVIERRFGLNGCEACTLEDLALELEVTRERVRQIQMESLRV
HHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LRQLLRRRGVTKDELF
HHHHHHHCCCCHHCCC
>Mature Secondary Structure 
SREHSDESDDLTPDETEADEERHAASDSNEELASAILDEPQVDVTQLYLNEIGQAPLLT
CCCCCCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHH
AAEEVALARRTVLGDFEARQTMIERNLRLVVNIAKHYANRGLTLLDLVEEGNLGLIHALE
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHCCCCHHHHHHH
KFDPERGFRFSTYATWWIRQNIERAIMNQSRTIRLPVHIIKELNIYLRAQRHLETHGVTD
HCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCC
ATSDDIAALTGHPVADVRRIMQLNERIASLDAPLDVDPTLSLGEAIADDNAHLPDEMLQL
CCCCCCHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHH
EEIENHVQEWLTQLNDKQRWVIERRFGLNGCEACTLEDLALELEVTRERVRQIQMESLRV
HHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LRQLLRRRGVTKDELF
HHHHHHHCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7729893 [H]