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The map label for this gene is nadE [H]

Identifier: 74316825

GI number: 74316825

Start: 848823

End: 850436

Strand: Direct

Name: nadE [H]

Synonym: Tbd_0807

Alternate gene names: 74316825

Gene position: 848823-850436 (Clockwise)

Preceding gene: 74316824

Following gene: 74316826

Centisome position: 29.17

GC content: 68.71

Gene sequence:

>1614_bases
ATGAAGCTCGCCCTCGCGCAGTTCAATTTCACGGTCGGCGACATCGCCGGCAACGCCGCGAAGCTGCTCGACGCCGCGCA
GGCTGCGCACGCGGCCGGTGCGGCGCTGCTGCTGACGCCGGAGATGTCGATTTGCGGCTACCCGGCCGAAGACCTCGTCT
GCCGGCGCGATTTCACTGCGGCGTGCGCGGCGGCGGTCGAGAGCCTGGCCCGGCAGGCGCCCGCCGAACTCGCGCTCATC
GTCGGTTACCCCGAGCGCACCGCGGCGGGTGTCTACAACGCCGCGGCACTCCTGCGCGGCGGCCGCATCGAGCAGGTCTA
CCGCAAGCATCATCTGCCGAACCATTCGGTCTTCGACGAGCAACGGGTTTTCTGCCGCGGTGACGACGCCTGCGTGTTCG
AGTGCGCGGGGATACGCTTCGGCATCAACATCTGCGGCGACATCTGGGAGCCCGGTCCCGCCACCGGGGCGATGGAAGCC
GGCGCCGACTGGCTCCTGGTGCTGAACGCCTCGCCCTACCATCTGAGCAAGGAGCGTGACCGCATCGCGGTCGCGCGGGC
GCGGCAGATCGAAACGCGTCTGCCGATGGTCTACGCCAACCTCGTCGGCGGTCAGGACGAACTCGTGTTCGACGGCGCCT
CGTTCGTGTTCGACGCCGACGGCGAAGTCGCCGTGCAATGCCCCGCGTGGCGCGAAGGCCTGTTCTATCTCGAACTCGAC
GCCGACGGCTGCAGCGGGCCGCGACATGAATTGCCCGGCGAGGAGGCCGCGGTGTACGAGGCGCTCGTGCTCGCCGTGCG
CGACTACGTCGGCAAGAACGGCTTCCGCGGCGTGCACCTGGGGCTCTCCGGCGGCATCGATTCGGCGCTCACGCTTGCGA
TCGCGGCCGACGCGGTCGGCGCCGAGCGCGTGCACGCGGTCATGATGCCGTCGGACTACACGGCCGGGATCAGCGTCGAG
GACTCGCGCGAGATCGTCGAGCGGCTCGGCGTCCGCTACGCCGAAATCGCGATCGGACCGATCGTCGAGGCATTCACGGG
TCAACTGGCAGGCGAGTTTGCGGGGCTCGCGAGCGACACGACCGAGGAAAACCTGCAGGCGCGCGCCCGCGGCACGCTGC
TGATGGCGCTCTCCAACAAGTTCGGCACGCTGGTGCTGACGACCGGCAACAAGAGCGAAATGGGGACCGGTTACGCGACG
CTCTACGGCGACATGGCCGGCGGCTTCGCCGTGTTGAAGGACGTCGCCAAGACGCGCGTCTACCGGCTCGCGAACTACCG
CAACAGCGTGTCGCCGGTGATCCCGCAGCGCATCATAGACCGTCCGCCGTCGGCCGAACTGCGCCCCGACCAGACCGACC
AGGACAGCCTGCCGCCTTACGAGGTGCTCGACGCGATCCTCGCCGCCTACGTCGAGCGCGATCTGCCGGCGCGCGAGATC
GTCGCGCAGGGCCACGACGCCGCGGTCGTGAAGAAGGTCATCCGCATGGTCGACCTCGCCGAATACAAGCGCCGCCAGTC
GCCGCCCGGCCCGCGCATCACGCCGCGCAATTTCGGCAAGGATCGCCGCTATCCGATCACCTCGAAATACCGTCCTGAAG
GAGAACTGCCGTGA

Upstream 100 bases:

>100_bases
AAAAATCCGCGCACTCGAAGCCGCGGGCGTCGTCGTCGCGCAGAGTCCGGCAGGCCTCGGCGAAGCAGTGCAGCTTGCGC
TCGGCGCCCGCTAGGGTGCG

Downstream 100 bases:

>100_bases
AAAAAATAGAAGCGATCATCAAGCCGTTCAAACTCGACGAAGTGCGCGAAGCGCTGTCCGAAATCGGCGTCACCGGGCTG
ACCGTGACCGAAGTCAAGGG

Product: NH(3)-dependent NAD(+) synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolysing] [H]

Number of amino acids: Translated: 537; Mature: 537

Protein sequence:

>537_residues
MKLALAQFNFTVGDIAGNAAKLLDAAQAAHAAGAALLLTPEMSICGYPAEDLVCRRDFTAACAAAVESLARQAPAELALI
VGYPERTAAGVYNAAALLRGGRIEQVYRKHHLPNHSVFDEQRVFCRGDDACVFECAGIRFGINICGDIWEPGPATGAMEA
GADWLLVLNASPYHLSKERDRIAVARARQIETRLPMVYANLVGGQDELVFDGASFVFDADGEVAVQCPAWREGLFYLELD
ADGCSGPRHELPGEEAAVYEALVLAVRDYVGKNGFRGVHLGLSGGIDSALTLAIAADAVGAERVHAVMMPSDYTAGISVE
DSREIVERLGVRYAEIAIGPIVEAFTGQLAGEFAGLASDTTEENLQARARGTLLMALSNKFGTLVLTTGNKSEMGTGYAT
LYGDMAGGFAVLKDVAKTRVYRLANYRNSVSPVIPQRIIDRPPSAELRPDQTDQDSLPPYEVLDAILAAYVERDLPAREI
VAQGHDAAVVKKVIRMVDLAEYKRRQSPPGPRITPRNFGKDRRYPITSKYRPEGELP

Sequences:

>Translated_537_residues
MKLALAQFNFTVGDIAGNAAKLLDAAQAAHAAGAALLLTPEMSICGYPAEDLVCRRDFTAACAAAVESLARQAPAELALI
VGYPERTAAGVYNAAALLRGGRIEQVYRKHHLPNHSVFDEQRVFCRGDDACVFECAGIRFGINICGDIWEPGPATGAMEA
GADWLLVLNASPYHLSKERDRIAVARARQIETRLPMVYANLVGGQDELVFDGASFVFDADGEVAVQCPAWREGLFYLELD
ADGCSGPRHELPGEEAAVYEALVLAVRDYVGKNGFRGVHLGLSGGIDSALTLAIAADAVGAERVHAVMMPSDYTAGISVE
DSREIVERLGVRYAEIAIGPIVEAFTGQLAGEFAGLASDTTEENLQARARGTLLMALSNKFGTLVLTTGNKSEMGTGYAT
LYGDMAGGFAVLKDVAKTRVYRLANYRNSVSPVIPQRIIDRPPSAELRPDQTDQDSLPPYEVLDAILAAYVERDLPAREI
VAQGHDAAVVKKVIRMVDLAEYKRRQSPPGPRITPRNFGKDRRYPITSKYRPEGELP
>Mature_537_residues
MKLALAQFNFTVGDIAGNAAKLLDAAQAAHAAGAALLLTPEMSICGYPAEDLVCRRDFTAACAAAVESLARQAPAELALI
VGYPERTAAGVYNAAALLRGGRIEQVYRKHHLPNHSVFDEQRVFCRGDDACVFECAGIRFGINICGDIWEPGPATGAMEA
GADWLLVLNASPYHLSKERDRIAVARARQIETRLPMVYANLVGGQDELVFDGASFVFDADGEVAVQCPAWREGLFYLELD
ADGCSGPRHELPGEEAAVYEALVLAVRDYVGKNGFRGVHLGLSGGIDSALTLAIAADAVGAERVHAVMMPSDYTAGISVE
DSREIVERLGVRYAEIAIGPIVEAFTGQLAGEFAGLASDTTEENLQARARGTLLMALSNKFGTLVLTTGNKSEMGTGYAT
LYGDMAGGFAVLKDVAKTRVYRLANYRNSVSPVIPQRIIDRPPSAELRPDQTDQDSLPPYEVLDAILAAYVERDLPAREI
VAQGHDAAVVKKVIRMVDLAEYKRRQSPPGPRITPRNFGKDRRYPITSKYRPEGELP

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR000132
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 57882; Mature: 57882

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLALAQFNFTVGDIAGNAAKLLDAAQAAHAAGAALLLTPEMSICGYPAEDLVCRRDFTA
CCEEEEECCCEEHHHCCCHHHHHHHHHHHHHCCEEEEEECCHHHCCCCHHHHHHHCCHHH
ACAAAVESLARQAPAELALIVGYPERTAAGVYNAAALLRGGRIEQVYRKHHLPNHSVFDE
HHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCC
QRVFCRGDDACVFECAGIRFGINICGDIWEPGPATGAMEAGADWLLVLNASPYHLSKERD
CCEEECCCCCEEEEECCEEECCEECCCCCCCCCCCCCHHCCCCEEEEEECCCCCCCCCHH
RIAVARARQIETRLPMVYANLVGGQDELVFDGASFVFDADGEVAVQCPAWREGLFYLELD
HHHHHHHHHHHHHCCCHHHHHCCCCCCEEECCCCEEEECCCCEEEECCCCCCCEEEEEEC
ADGCSGPRHELPGEEAAVYEALVLAVRDYVGKNGFRGVHLGLSGGIDSALTLAIAADAVG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHCC
AERVHAVMMPSDYTAGISVEDSREIVERLGVRYAEIAIGPIVEAFTGQLAGEFAGLASDT
CHHEEEEECCCCCCCCCCCCCHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCC
TEENLQARARGTLLMALSNKFGTLVLTTGNKSEMGTGYATLYGDMAGGFAVLKDVAKTRV
CHHHHHHHHCCEEEEEECCCCCEEEEEECCCCCCCCCEEEEECCCCCHHHHHHHHHHHHH
YRLANYRNSVSPVIPQRIIDRPPSAELRPDQTDQDSLPPYEVLDAILAAYVERDLPAREI
HHHHHCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHH
VAQGHDAAVVKKVIRMVDLAEYKRRQSPPGPRITPRNFGKDRRYPITSKYRPEGELP
HHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKLALAQFNFTVGDIAGNAAKLLDAAQAAHAAGAALLLTPEMSICGYPAEDLVCRRDFTA
CCEEEEECCCEEHHHCCCHHHHHHHHHHHHHCCEEEEEECCHHHCCCCHHHHHHHCCHHH
ACAAAVESLARQAPAELALIVGYPERTAAGVYNAAALLRGGRIEQVYRKHHLPNHSVFDE
HHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCC
QRVFCRGDDACVFECAGIRFGINICGDIWEPGPATGAMEAGADWLLVLNASPYHLSKERD
CCEEECCCCCEEEEECCEEECCEECCCCCCCCCCCCCHHCCCCEEEEEECCCCCCCCCHH
RIAVARARQIETRLPMVYANLVGGQDELVFDGASFVFDADGEVAVQCPAWREGLFYLELD
HHHHHHHHHHHHHCCCHHHHHCCCCCCEEECCCCEEEECCCCEEEECCCCCCCEEEEEEC
ADGCSGPRHELPGEEAAVYEALVLAVRDYVGKNGFRGVHLGLSGGIDSALTLAIAADAVG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHCC
AERVHAVMMPSDYTAGISVEDSREIVERLGVRYAEIAIGPIVEAFTGQLAGEFAGLASDT
CHHEEEEECCCCCCCCCCCCCHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCC
TEENLQARARGTLLMALSNKFGTLVLTTGNKSEMGTGYATLYGDMAGGFAVLKDVAKTRV
CHHHHHHHHCCEEEEEECCCCCEEEEEECCCCCCCCCEEEEECCCCCHHHHHHHHHHHHH
YRLANYRNSVSPVIPQRIIDRPPSAELRPDQTDQDSLPPYEVLDAILAAYVERDLPAREI
HHHHHCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHH
VAQGHDAAVVKKVIRMVDLAEYKRRQSPPGPRITPRNFGKDRRYPITSKYRPEGELP
HHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]