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The map label for this gene is epsE [H]

Identifier: 74316770

GI number: 74316770

Start: 796193

End: 797926

Strand: Direct

Name: epsE [H]

Synonym: Tbd_0752

Alternate gene names: 74316770

Gene position: 796193-797926 (Clockwise)

Preceding gene: 74316769

Following gene: 74316771

Centisome position: 27.36

GC content: 70.42

Gene sequence:

>1734_bases
ATGCTGACCGAGACCGTTCCGCCGGCCGCCGCGCCCGCGGCGCGCCAGAAAATCCGCCTCGGCGATCTGCTCGTCGAACA
GAAGGTCATTTCGGCCGCCGACCTCGATATCGCGCTGACGGCGCAGAAGAAGAGCGGACGGCGGCTGGGCCGCATCATCG
TCGAGTCGGGCCTCGCCGGGGAGAACGACATCGCCCAGGCGCTGGCCCGCCAACTCGCGATTCCCTTCGTCGACCTCCGG
AAATTCAACCCCGACCCGTCGATCCTGCAGTTGCTCGGGGAGACCCAGGCGCGGCGCTTCCGCGCGATTCCGCTCGGCCG
GCGCGAAGGCGACATCTTCGTCGGCATGGCCGACCCGACCGACCTGTTCGCCTACGACGAGGTCGCGCGCCTGATCGAGG
GCGGCATCCAGCTCGCAGTCGTCGCCGAGGGCGATCTCCTGTCGGCGATCGACCGCCTGTACCGGCGCACCGACGACATC
CACGGCCTGACCGAGGAACTCGCACGCGACATGGGCGAGAGCGAGGCCAGCATCATCGGCCTCGACGCGCTCGGCGAAGG
CCAGGCCGACGCGCCGGTGGTGCGGCTGCTGCAGACGCTGTTCGAGGACGCACTGCAGGTCAACGCCTCGGACGTGCATA
TCGAGCCGCAGGAAAAGCAGCTCATGATCCGCTTTCGCATCGACGGCGTGCTGCATCGGCAGACCGAGGCCGACCTCAGG
ATCGCCCCCGCGCTGGCGCTGCGCCTGAAGATCGTCTCCGGGCTCGACATTTCGGAGAAGCGGCTGCCGCAGGACGGCCG
CTTCGCCGTCAAGGTGCGCGGCCGCACCGTCGACGTGCGCCTGTCGACGATGCCGACGCAATACGGCGAATCGGTCGTGA
TGCGCCTCCTGAGCCAGGGCGACAACGCGCCGACGCTCGACACGCTGGGCATGCCGCCCGCGATGCTCGAACGCTTCCGC
GCGATCCTGCACCGCCCCAACGGCCTCGTCCTCGTCACCGGCCCGACCGGCAGCGGCAAGACGACGACGCTCTATGCCGC
GCTCGGCGAACTCAACGACCCGGGCACCAAGATCATCACGGTCGAGGACCCGGTCGAATACCGCCTGCCCGGTGTCAACC
AGGTCCAGGCCAACGACAAGATCGACCTCAGCTTCGCGCGGGTGCTGCGCGCGATGCTGCGCCAGGACCCCGACGTCATC
CTGGTCGGCGAGATGCGCGACCCCGAGACTGCGCAGATCGCGCTGCGCGCCGCGATGACGGGCCATCTCGTACTCTCGAC
GCTGCACACCAACGACGCCGCGTCGACGCCGGTGCGCCTGCTCGACATGGGCGTGCCGTCGTTCATGGTCGCGACCTCGG
TTCAGGGCGTGCTTGCGCAGCGCCTGCTGCGCAAGGTCTGCGAACACTGCAAGGCGCCGCATGTGCCGACCCCCCAGGAA
CGCGTGTGGCTCGGCGCGGCCTATGCCGAGGGCGACGCCTACGTGGCGGGACGCGGCTGCGAGCGCTGCCACGGCACGGG
CCACGCCGGCCGCCAGGCGATCCACGAACTGCTCGAGATCAGCGCGCCGCTCGCCGACGCGCTCGCGCGCAAGCCGCCGG
CCGAGTTCGTCCTCGCCGCTCGCGCCGAACTCGCCGGCCGCACGCTGCGCGATCAGGCGCTCGCGCTCGCGCGCAGCGGC
GTCGCCTCGCTGTCCGAGGCGATGCGGGTCAGCGCGCAGGACGAGACGCACTGA

Upstream 100 bases:

>100_bases
TTTTCGCCGCGCGACCCGGCAGCCGGCCTGCGCGGCTCAAGGCGGCGCCCTCCGAAGCCGATACCAGCTCATCCCTCATA
GCCAAAACAAACCCAGAACC

Downstream 100 bases:

>100_bases
TGCGTTTCAGCTACACCGGCCGCACCCAGGGCGGCGAGAAGGCGCAGGGCATCCTCGAAGCAGAAAGCGCGACCGAATGC
GCGGCGAGCCTCCTGAAGAG

Product: pilus assembly pathway ATPase PilB

Products: NA

Alternate protein names: Cholera toxin secretion protein epsE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 577; Mature: 577

Protein sequence:

>577_residues
MLTETVPPAAAPAARQKIRLGDLLVEQKVISAADLDIALTAQKKSGRRLGRIIVESGLAGENDIAQALARQLAIPFVDLR
KFNPDPSILQLLGETQARRFRAIPLGRREGDIFVGMADPTDLFAYDEVARLIEGGIQLAVVAEGDLLSAIDRLYRRTDDI
HGLTEELARDMGESEASIIGLDALGEGQADAPVVRLLQTLFEDALQVNASDVHIEPQEKQLMIRFRIDGVLHRQTEADLR
IAPALALRLKIVSGLDISEKRLPQDGRFAVKVRGRTVDVRLSTMPTQYGESVVMRLLSQGDNAPTLDTLGMPPAMLERFR
AILHRPNGLVLVTGPTGSGKTTTLYAALGELNDPGTKIITVEDPVEYRLPGVNQVQANDKIDLSFARVLRAMLRQDPDVI
LVGEMRDPETAQIALRAAMTGHLVLSTLHTNDAASTPVRLLDMGVPSFMVATSVQGVLAQRLLRKVCEHCKAPHVPTPQE
RVWLGAAYAEGDAYVAGRGCERCHGTGHAGRQAIHELLEISAPLADALARKPPAEFVLAARAELAGRTLRDQALALARSG
VASLSEAMRVSAQDETH

Sequences:

>Translated_577_residues
MLTETVPPAAAPAARQKIRLGDLLVEQKVISAADLDIALTAQKKSGRRLGRIIVESGLAGENDIAQALARQLAIPFVDLR
KFNPDPSILQLLGETQARRFRAIPLGRREGDIFVGMADPTDLFAYDEVARLIEGGIQLAVVAEGDLLSAIDRLYRRTDDI
HGLTEELARDMGESEASIIGLDALGEGQADAPVVRLLQTLFEDALQVNASDVHIEPQEKQLMIRFRIDGVLHRQTEADLR
IAPALALRLKIVSGLDISEKRLPQDGRFAVKVRGRTVDVRLSTMPTQYGESVVMRLLSQGDNAPTLDTLGMPPAMLERFR
AILHRPNGLVLVTGPTGSGKTTTLYAALGELNDPGTKIITVEDPVEYRLPGVNQVQANDKIDLSFARVLRAMLRQDPDVI
LVGEMRDPETAQIALRAAMTGHLVLSTLHTNDAASTPVRLLDMGVPSFMVATSVQGVLAQRLLRKVCEHCKAPHVPTPQE
RVWLGAAYAEGDAYVAGRGCERCHGTGHAGRQAIHELLEISAPLADALARKPPAEFVLAARAELAGRTLRDQALALARSG
VASLSEAMRVSAQDETH
>Mature_577_residues
MLTETVPPAAAPAARQKIRLGDLLVEQKVISAADLDIALTAQKKSGRRLGRIIVESGLAGENDIAQALARQLAIPFVDLR
KFNPDPSILQLLGETQARRFRAIPLGRREGDIFVGMADPTDLFAYDEVARLIEGGIQLAVVAEGDLLSAIDRLYRRTDDI
HGLTEELARDMGESEASIIGLDALGEGQADAPVVRLLQTLFEDALQVNASDVHIEPQEKQLMIRFRIDGVLHRQTEADLR
IAPALALRLKIVSGLDISEKRLPQDGRFAVKVRGRTVDVRLSTMPTQYGESVVMRLLSQGDNAPTLDTLGMPPAMLERFR
AILHRPNGLVLVTGPTGSGKTTTLYAALGELNDPGTKIITVEDPVEYRLPGVNQVQANDKIDLSFARVLRAMLRQDPDVI
LVGEMRDPETAQIALRAAMTGHLVLSTLHTNDAASTPVRLLDMGVPSFMVATSVQGVLAQRLLRKVCEHCKAPHVPTPQE
RVWLGAAYAEGDAYVAGRGCERCHGTGHAGRQAIHELLEISAPLADALARKPPAEFVLAARAELAGRTLRDQALALARSG
VASLSEAMRVSAQDETH

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=427, Percent_Identity=43.7939110070258, Blast_Score=349, Evalue=2e-97,
Organism=Escherichia coli, GI1786296, Length=385, Percent_Identity=45.1948051948052, Blast_Score=301, Evalue=6e-83,
Organism=Escherichia coli, GI87082188, Length=209, Percent_Identity=44.0191387559809, Blast_Score=130, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 62485; Mature: 62485

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTETVPPAAAPAARQKIRLGDLLVEQKVISAADLDIALTAQKKSGRRLGRIIVESGLAG
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCC
ENDIAQALARQLAIPFVDLRKFNPDPSILQLLGETQARRFRAIPLGRREGDIFVGMADPT
CHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHHHEECCCCCCCCCCEEEEECCCC
DLFAYDEVARLIEGGIQLAVVAEGDLLSAIDRLYRRTDDIHGLTEELARDMGESEASIIG
HHHHHHHHHHHHHCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
LDALGEGQADAPVVRLLQTLFEDALQVNASDVHIEPQEKQLMIRFRIDGVLHRQTEADLR
EECCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCEEEEEEEECCHHCCCCCCCEE
IAPALALRLKIVSGLDISEKRLPQDGRFAVKVRGRTVDVRLSTMPTQYGESVVMRLLSQG
EHHHHHHHHHHHCCCCCHHHHCCCCCCEEEEEECEEEEEEEECCCCHHHHHHHHHHHHCC
DNAPTLDTLGMPPAMLERFRAILHRPNGLVLVTGPTGSGKTTTLYAALGELNDPGTKIIT
CCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEECCCCCCCCCEEEE
VEDPVEYRLPGVNQVQANDKIDLSFARVLRAMLRQDPDVILVGEMRDPETAQIALRAAMT
ECCCHHCCCCCCCEECCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHH
GHLVLSTLHTNDAASTPVRLLDMGVPSFMVATSVQGVLAQRLLRKVCEHCKAPHVPTPQE
HHEEEEEEECCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
RVWLGAAYAEGDAYVAGRGCERCHGTGHAGRQAIHELLEISAPLADALARKPPAEFVLAA
HEEEEEEEECCCEEEECCCHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHH
RAELAGRTLRDQALALARSGVASLSEAMRVSAQDETH
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MLTETVPPAAAPAARQKIRLGDLLVEQKVISAADLDIALTAQKKSGRRLGRIIVESGLAG
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCC
ENDIAQALARQLAIPFVDLRKFNPDPSILQLLGETQARRFRAIPLGRREGDIFVGMADPT
CHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHHHEECCCCCCCCCCEEEEECCCC
DLFAYDEVARLIEGGIQLAVVAEGDLLSAIDRLYRRTDDIHGLTEELARDMGESEASIIG
HHHHHHHHHHHHHCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
LDALGEGQADAPVVRLLQTLFEDALQVNASDVHIEPQEKQLMIRFRIDGVLHRQTEADLR
EECCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCEEEEEEEECCHHCCCCCCCEE
IAPALALRLKIVSGLDISEKRLPQDGRFAVKVRGRTVDVRLSTMPTQYGESVVMRLLSQG
EHHHHHHHHHHHCCCCCHHHHCCCCCCEEEEEECEEEEEEEECCCCHHHHHHHHHHHHCC
DNAPTLDTLGMPPAMLERFRAILHRPNGLVLVTGPTGSGKTTTLYAALGELNDPGTKIIT
CCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEECCCCCCCCCEEEE
VEDPVEYRLPGVNQVQANDKIDLSFARVLRAMLRQDPDVILVGEMRDPETAQIALRAAMT
ECCCHHCCCCCCCEECCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHH
GHLVLSTLHTNDAASTPVRLLDMGVPSFMVATSVQGVLAQRLLRKVCEHCKAPHVPTPQE
HHEEEEEEECCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
RVWLGAAYAEGDAYVAGRGCERCHGTGHAGRQAIHELLEISAPLADALARKPPAEFVLAA
HEEEEEEEECCCEEEECCCHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHH
RAELAGRTLRDQALALARSGVASLSEAMRVSAQDETH
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8423007; 10952301 [H]