Click here to switch to the map view.

The map label for this gene is nusA [H]

Identifier: 74316716

GI number: 74316716

Start: 739006

End: 740484

Strand: Reverse

Name: nusA [H]

Synonym: Tbd_0698

Alternate gene names: 74316716

Gene position: 740484-739006 (Counterclockwise)

Preceding gene: 74316717

Following gene: 74316715

Centisome position: 25.45

GC content: 64.71

Gene sequence:

>1479_bases
ATGAGCCGTGAGATGTTGATGCTGGCCGACGCGCTGGCGCGCGAAAAGAACGTGGACAAGGAGGTGGTGTTCGAGGCCCT
CGAGCAGGCGCTCGCCTCGGCCACCAAGAAGCGCTTCAAGGAAGAGTCCGACGTGCGCGTCGCGATCGATCGCGAAACCG
GTGACTACGAATCCTTCCGTCGCTGGCTGGTCGTGACCGAGGCCGAGCTCGAATCCGAGGGCTACCAGATCCTGCTGGTC
GACGCCCAGGACAAGATCCCGGACATCGAAATCGGCGACTACATCGAAGAGCCGCTCGAAAACGTCGAGTTCGGCCGCAT
CGGTGCCCAGGCCGCCAAGCAGGTCATCCTGCAGAAGATCCGCGATGCCGAGCGCGAGCAGATCATCAGCGACTTCCTCG
CCCGCAAGGAACACCTCGTCAATGGCGTCGTGAAGCGCATCGACCGTGGCAACGCGATCATCGAATCCGGCCGCGTCGAA
GGCTTCCTGCACCGCGACCAGATGATCCCGCGCGAGAACCTGCGTGTCGGCGACCGCGTGCGCGCCTACCTGCTGCGCAT
CGACCGCGGCAACCGCGGCCCGCAGGTCGTGCTGTCGCGCACCGCCCCAGAATTCATCATGAAGCTGTTCGAGCTCGAAG
TGCCCGAGATCGAGGAAGGCCTGCTCGAGATCAAGGCCGCGGCCCGTGATCCCGGCCTGCGCGCCAAGATCGCCGTCGTC
TCGCACGATCCGCGCATCGACCCGATCGGCACCTGCATCGGCCTTCGTGGGTCGCGCGTCACCTCGGTGACCAACGAACT
CGCCGGCGAGCGCGTCGACATCATCCACTGGTCGGCCGATCCGGCACAGTACGTGATCAATGCCCTCGCGCCGGCCGAAG
TCAGCTCGATCGTCGTCGACGAAGATACGCACAGCATGGACGTCGTCGTCGACGAGGAACAACTCGCGATGGCGATCGGC
CGCGGTGGCCAGAACGTGCGCCTGGCGTCCGAACTGACCGGCTGGGAACTCAACATCATGTCGCGCGAGGCGGCTGAAGA
GAAACAGTCGAGCGAAAGCCAGAAGACGCTGCAGCTCTTCATCGAGAAGCTCGACGTCGACGAGGAAGTCGCCCAGATTC
TGGTCGACGAGGGCTTCTCCACGCTCGAGGAAGTCGCCTACGTGCCGCTCAACGAAATGCTCGAGATCGAAGCCTTCGAT
GAAGCGCTCGTCAACGAACTGCGCAACCGGGCGCGCAACGCCCTGCTGACCGCGGCCATCGTCGGCGAGGAGCAGGTCGA
GGCCTCGGCCGGCGACCTGCTGTCGCTCGACGGCATGGACGCCGAAACCGCACGCTTGCTTGCCAGCAAGGGGGTCCACA
CGACCGAGGATCTGGCGGAGCTGGCGGTCGACGAGCTGACCGAAATGGCCGCGATGGACGCGGAACGCGCCAAACAATTG
ATCATGGCCGCACGCGCGCCCTGGTTCGCCCAAGGCTAA

Upstream 100 bases:

>100_bases
GGTGAAATTGGTCGTCGATGGCCGCGAAATGTCGGTCGATTTGAGAAATGTGGACGCTGCCCGCCTCAAGCCGATGGTTT
GAAGCGAAGCTGGAGAGAAT

Downstream 100 bases:

>100_bases
GAGGATTGCATGAACGTTGAACAATTCGCCCAGGAACTGAAACTGCCGCCCCAGCTGTTGCTCGAACAGCTGAAAGCGGC
GGGCGTCAGCAAGAATGATG

Product: NusA antitermination factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 492; Mature: 491

Protein sequence:

>492_residues
MSREMLMLADALAREKNVDKEVVFEALEQALASATKKRFKEESDVRVAIDRETGDYESFRRWLVVTEAELESEGYQILLV
DAQDKIPDIEIGDYIEEPLENVEFGRIGAQAAKQVILQKIRDAEREQIISDFLARKEHLVNGVVKRIDRGNAIIESGRVE
GFLHRDQMIPRENLRVGDRVRAYLLRIDRGNRGPQVVLSRTAPEFIMKLFELEVPEIEEGLLEIKAAARDPGLRAKIAVV
SHDPRIDPIGTCIGLRGSRVTSVTNELAGERVDIIHWSADPAQYVINALAPAEVSSIVVDEDTHSMDVVVDEEQLAMAIG
RGGQNVRLASELTGWELNIMSREAAEEKQSSESQKTLQLFIEKLDVDEEVAQILVDEGFSTLEEVAYVPLNEMLEIEAFD
EALVNELRNRARNALLTAAIVGEEQVEASAGDLLSLDGMDAETARLLASKGVHTTEDLAELAVDELTEMAAMDAERAKQL
IMAARAPWFAQG

Sequences:

>Translated_492_residues
MSREMLMLADALAREKNVDKEVVFEALEQALASATKKRFKEESDVRVAIDRETGDYESFRRWLVVTEAELESEGYQILLV
DAQDKIPDIEIGDYIEEPLENVEFGRIGAQAAKQVILQKIRDAEREQIISDFLARKEHLVNGVVKRIDRGNAIIESGRVE
GFLHRDQMIPRENLRVGDRVRAYLLRIDRGNRGPQVVLSRTAPEFIMKLFELEVPEIEEGLLEIKAAARDPGLRAKIAVV
SHDPRIDPIGTCIGLRGSRVTSVTNELAGERVDIIHWSADPAQYVINALAPAEVSSIVVDEDTHSMDVVVDEEQLAMAIG
RGGQNVRLASELTGWELNIMSREAAEEKQSSESQKTLQLFIEKLDVDEEVAQILVDEGFSTLEEVAYVPLNEMLEIEAFD
EALVNELRNRARNALLTAAIVGEEQVEASAGDLLSLDGMDAETARLLASKGVHTTEDLAELAVDELTEMAAMDAERAKQL
IMAARAPWFAQG
>Mature_491_residues
SREMLMLADALAREKNVDKEVVFEALEQALASATKKRFKEESDVRVAIDRETGDYESFRRWLVVTEAELESEGYQILLVD
AQDKIPDIEIGDYIEEPLENVEFGRIGAQAAKQVILQKIRDAEREQIISDFLARKEHLVNGVVKRIDRGNAIIESGRVEG
FLHRDQMIPRENLRVGDRVRAYLLRIDRGNRGPQVVLSRTAPEFIMKLFELEVPEIEEGLLEIKAAARDPGLRAKIAVVS
HDPRIDPIGTCIGLRGSRVTSVTNELAGERVDIIHWSADPAQYVINALAPAEVSSIVVDEDTHSMDVVVDEEQLAMAIGR
GGQNVRLASELTGWELNIMSREAAEEKQSSESQKTLQLFIEKLDVDEEVAQILVDEGFSTLEEVAYVPLNEMLEIEAFDE
ALVNELRNRARNALLTAAIVGEEQVEASAGDLLSLDGMDAETARLLASKGVHTTEDLAELAVDELTEMAAMDAERAKQLI
MAARAPWFAQG

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=494, Percent_Identity=50.2024291497976, Blast_Score=489, Evalue=1e-139,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010995
- InterPro:   IPR003583
- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010214
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 54750; Mature: 54618

Theoretical pI: Translated: 4.28; Mature: 4.28

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSREMLMLADALAREKNVDKEVVFEALEQALASATKKRFKEESDVRVAIDRETGDYESFR
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHH
RWLVVTEAELESEGYQILLVDAQDKIPDIEIGDYIEEPLENVEFGRIGAQAAKQVILQKI
HEEEEEEHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
RDAEREQIISDFLARKEHLVNGVVKRIDRGNAIIESGRVEGFLHRDQMIPRENLRVGDRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCEEHHHHCCCCCCCCHHHHH
RAYLLRIDRGNRGPQVVLSRTAPEFIMKLFELEVPEIEEGLLEIKAAARDPGLRAKIAVV
HHHHHEECCCCCCCEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEEEE
SHDPRIDPIGTCIGLRGSRVTSVTNELAGERVDIIHWSADPAQYVINALAPAEVSSIVVD
ECCCCCCHHHHHHCCCCCHHHHHHHHHCCCEEEEEEECCCHHHHHHHHCCCHHHHEEEEE
EDTHSMDVVVDEEQLAMAIGRGGQNVRLASELTGWELNIMSREAAEEKQSSESQKTLQLF
CCCCCEEEEECHHHHHHHHCCCCCCEEEHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHH
IEKLDVDEEVAQILVDEGFSTLEEVAYVPLNEMLEIEAFDEALVNELRNRARNALLTAAI
HHHHCCHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGEEQVEASAGDLLSLDGMDAETARLLASKGVHTTEDLAELAVDELTEMAAMDAERAKQL
HCHHHHHCCCCCEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IMAARAPWFAQG
HHHHCCCCCCCC
>Mature Secondary Structure 
SREMLMLADALAREKNVDKEVVFEALEQALASATKKRFKEESDVRVAIDRETGDYESFR
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHH
RWLVVTEAELESEGYQILLVDAQDKIPDIEIGDYIEEPLENVEFGRIGAQAAKQVILQKI
HEEEEEEHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
RDAEREQIISDFLARKEHLVNGVVKRIDRGNAIIESGRVEGFLHRDQMIPRENLRVGDRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCEEHHHHCCCCCCCCHHHHH
RAYLLRIDRGNRGPQVVLSRTAPEFIMKLFELEVPEIEEGLLEIKAAARDPGLRAKIAVV
HHHHHEECCCCCCCEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEEEE
SHDPRIDPIGTCIGLRGSRVTSVTNELAGERVDIIHWSADPAQYVINALAPAEVSSIVVD
ECCCCCCHHHHHHCCCCCHHHHHHHHHCCCEEEEEEECCCHHHHHHHHCCCHHHHEEEEE
EDTHSMDVVVDEEQLAMAIGRGGQNVRLASELTGWELNIMSREAAEEKQSSESQKTLQLF
CCCCCEEEEECHHHHHHHHCCCCCCEEEHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHH
IEKLDVDEEVAQILVDEGFSTLEEVAYVPLNEMLEIEAFDEALVNELRNRARNALLTAAI
HHHHCCHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGEEQVEASAGDLLSLDGMDAETARLLASKGVHTTEDLAELAVDELTEMAAMDAERAKQL
HCHHHHHCCCCCEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IMAARAPWFAQG
HHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12704232 [H]