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The map label for this gene is folD

Identifier: 74316707

GI number: 74316707

Start: 729447

End: 730301

Strand: Direct

Name: folD

Synonym: Tbd_0689

Alternate gene names: 74316707

Gene position: 729447-730301 (Clockwise)

Preceding gene: 74316705

Following gene: 74316709

Centisome position: 25.07

GC content: 67.37

Gene sequence:

>855_bases
ATGAGCGCACGCATTCTCGACGGCAAGGCGATGGCCGACACCATTCTCGCAGTGATCCACGACAAGGTGGCCGAGCGCGA
GGTGCAGGGCAAACGCCGCCCGGGGCTTGCGGTGATCCTCGTCGGGGGCGATCCGGCGTCGGCGGTCTACGTGCGCAACA
AGAAGCGCGCCTGCGAACGCGCCGGCGTCAACAGCGTGTCGCACGATCTGCCCGAGACGACGACCCAGCCGGAACTGCTC
GCGCTGATTGACACGCTCAACAGCGATGCCGCGATCGACGGCATCCTCGTGCAGTTGCCGCTGCCCGTTCACATCGACGC
CGAGACCGTGATCGAACGCATTCGGCCGGACAAGGACGTCGACGGCTTCCACCCCTACAACATCGGCCGCCTCGCGGTGA
AGATGCCGACGCTGCGCCCGTCGACGCCGCGGGGCATCATGACCTTGCTGCGCGCGACCAACGAGGAGCTGCGCGGCAAG
AACGCGGTCATGGTCGGCGCATCCAACATCGTCGGCCGACCGATGAGCCTCGAACTGCTTCTGGCCGGTTGCACGATCAC
GGTCTGCCACAGCGCGACGCGCGATCTGGAGAGCTTCGTTCGTTCGGCCGAAGTGCTGGTCGTCGGGGTCGGTCGCCCGC
GCATGATCCCGGGCGACTGGGTGCGCGAGGGGGCGATCGTGATCGACGTCGGCATCAATCGCCTGGCCGACGGCAAGCTC
GTCGGCGACGTCGATTTCGATACGGCGGTCGAACGCGCCGGGTGGATCACCCCGGTGCCGGGCGGGGTCGGGCCGATGAC
CGTCGCGACGCTGCTCGAAAACACGCTCGAAGCCGCGCTGATGCACAATCCCTAG

Upstream 100 bases:

>100_bases
GAGGCGAGACTATACCTTGGCCGATCAACCGGGTCAATTTTCGCTTGAGCAAAGTGGGCGGTTTCCGGGAAAATCCCCCT
TTTGCTTCAGGGTCCTCTTG

Downstream 100 bases:

>100_bases
GCGCTAGGCGCTAGGCGCTAGGCGCTAGGCGCTAGGCGCTAGGGGCTAGGGGCCAGGGGCTGGGGGTCAGGGGTGGCGCT
AATTCGGCTTCGCTCCCTGA

Product: methenyltetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MSARILDGKAMADTILAVIHDKVAEREVQGKRRPGLAVILVGGDPASAVYVRNKKRACERAGVNSVSHDLPETTTQPELL
ALIDTLNSDAAIDGILVQLPLPVHIDAETVIERIRPDKDVDGFHPYNIGRLAVKMPTLRPSTPRGIMTLLRATNEELRGK
NAVMVGASNIVGRPMSLELLLAGCTITVCHSATRDLESFVRSAEVLVVGVGRPRMIPGDWVREGAIVIDVGINRLADGKL
VGDVDFDTAVERAGWITPVPGGVGPMTVATLLENTLEAALMHNP

Sequences:

>Translated_284_residues
MSARILDGKAMADTILAVIHDKVAEREVQGKRRPGLAVILVGGDPASAVYVRNKKRACERAGVNSVSHDLPETTTQPELL
ALIDTLNSDAAIDGILVQLPLPVHIDAETVIERIRPDKDVDGFHPYNIGRLAVKMPTLRPSTPRGIMTLLRATNEELRGK
NAVMVGASNIVGRPMSLELLLAGCTITVCHSATRDLESFVRSAEVLVVGVGRPRMIPGDWVREGAIVIDVGINRLADGKL
VGDVDFDTAVERAGWITPVPGGVGPMTVATLLENTLEAALMHNP
>Mature_283_residues
SARILDGKAMADTILAVIHDKVAEREVQGKRRPGLAVILVGGDPASAVYVRNKKRACERAGVNSVSHDLPETTTQPELLA
LIDTLNSDAAIDGILVQLPLPVHIDAETVIERIRPDKDVDGFHPYNIGRLAVKMPTLRPSTPRGIMTLLRATNEELRGKN
AVMVGASNIVGRPMSLELLLAGCTITVCHSATRDLESFVRSAEVLVVGVGRPRMIPGDWVREGAIVIDVGINRLADGKLV
GDVDFDTAVERAGWITPVPGGVGPMTVATLLENTLEAALMHNP

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222418558, Length=292, Percent_Identity=45.5479452054795, Blast_Score=223, Evalue=1e-58,
Organism=Homo sapiens, GI222136639, Length=288, Percent_Identity=43.4027777777778, Blast_Score=218, Evalue=5e-57,
Organism=Homo sapiens, GI94721354, Length=292, Percent_Identity=43.4931506849315, Blast_Score=214, Evalue=7e-56,
Organism=Homo sapiens, GI36796743, Length=206, Percent_Identity=25.2427184466019, Blast_Score=72, Evalue=7e-13,
Organism=Escherichia coli, GI1786741, Length=285, Percent_Identity=62.8070175438596, Blast_Score=363, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI17568735, Length=290, Percent_Identity=41.0344827586207, Blast_Score=190, Evalue=6e-49,
Organism=Saccharomyces cerevisiae, GI6319558, Length=285, Percent_Identity=44.9122807017544, Blast_Score=240, Evalue=2e-64,
Organism=Saccharomyces cerevisiae, GI6321643, Length=298, Percent_Identity=42.6174496644295, Blast_Score=232, Evalue=7e-62,
Organism=Drosophila melanogaster, GI17136816, Length=292, Percent_Identity=47.2602739726027, Blast_Score=242, Evalue=2e-64,
Organism=Drosophila melanogaster, GI17136818, Length=292, Percent_Identity=47.2602739726027, Blast_Score=242, Evalue=2e-64,
Organism=Drosophila melanogaster, GI24645718, Length=287, Percent_Identity=43.5540069686411, Blast_Score=223, Evalue=1e-58,
Organism=Drosophila melanogaster, GI17137370, Length=287, Percent_Identity=43.5540069686411, Blast_Score=223, Evalue=1e-58,
Organism=Drosophila melanogaster, GI62472483, Length=287, Percent_Identity=43.5540069686411, Blast_Score=223, Evalue=2e-58,
Organism=Drosophila melanogaster, GI45551871, Length=287, Percent_Identity=43.5540069686411, Blast_Score=223, Evalue=2e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_THIDA (Q3SKX9)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_314447.1
- HSSP:   O50385
- ProteinModelPortal:   Q3SKX9
- SMR:   Q3SKX9
- STRING:   Q3SKX9
- GeneID:   3672657
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_0689
- NMPDR:   fig|292415.3.peg.718
- eggNOG:   COG0190
- HOGENOM:   HBG328751
- OMA:   LLEHYHI
- PhylomeDB:   Q3SKX9
- BioCyc:   TDEN292415:TBD_0689-MONOMER
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 30386; Mature: 30254

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSARILDGKAMADTILAVIHDKVAEREVQGKRRPGLAVILVGGDPASAVYVRNKKRACER
CCCEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEEEECHHHHHHH
AGVNSVSHDLPETTTQPELLALIDTLNSDAAIDGILVQLPLPVHIDAETVIERIRPDKDV
CCCCHHHHCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCEEECHHHHHHHHCCCCCC
DGFHPYNIGRLAVKMPTLRPSTPRGIMTLLRATNEELRGKNAVMVGASNIVGRPMSLELL
CCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECHHHCCCCCEEEEE
LAGCTITVCHSATRDLESFVRSAEVLVVGVGRPRMIPGDWVREGAIVIDVGINRLADGKL
EECCEEEEHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHCCCEEEEECCCCCCCCCE
VGDVDFDTAVERAGWITPVPGGVGPMTVATLLENTLEAALMHNP
EECCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SARILDGKAMADTILAVIHDKVAEREVQGKRRPGLAVILVGGDPASAVYVRNKKRACER
CCEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEEEECHHHHHHH
AGVNSVSHDLPETTTQPELLALIDTLNSDAAIDGILVQLPLPVHIDAETVIERIRPDKDV
CCCCHHHHCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCEEECHHHHHHHHCCCCCC
DGFHPYNIGRLAVKMPTLRPSTPRGIMTLLRATNEELRGKNAVMVGASNIVGRPMSLELL
CCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECHHHCCCCCEEEEE
LAGCTITVCHSATRDLESFVRSAEVLVVGVGRPRMIPGDWVREGAIVIDVGINRLADGKL
EECCEEEEHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHCCCEEEEECCCCCCCCCE
VGDVDFDTAVERAGWITPVPGGVGPMTVATLLENTLEAALMHNP
EECCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA