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The map label for this gene is ywoG [H]

Identifier: 74316692

GI number: 74316692

Start: 714616

End: 715800

Strand: Reverse

Name: ywoG [H]

Synonym: Tbd_0674

Alternate gene names: 74316692

Gene position: 715800-714616 (Counterclockwise)

Preceding gene: 74316699

Following gene: 74316691

Centisome position: 24.6

GC content: 67.85

Gene sequence:

>1185_bases
ATGAAGTCCGCACGCCCCTCCTTCTTCGTCCTCATCGCGCTGGTCGGCGGTCTCGCGATCTTCAGCTCGACTCTGTCGAA
GACACCGGTCCTGCCGCTGTTCGCCCAGGCACTCGACGCGACGCCTGCCGAAATCGGCTGGATCGTCATGGCCTCGACGA
TCCCCGGCATCCTGATCAGCTACCCGGCAGGCGCGCTCTCGGACCAGCTCGGCAAGCGCCGCGTGCTGCTCGCCTCACTG
GTCGTTTTCGCCACCGCGCCCTTTCTTTATCTGATCATCAGCACGGCCTGGCAACTCATGGCCGTCCGCTTCTACCACGG
CTTCGCCACGGCAATCTTCGGCACCGTCGCGAGCGCCGCGATCGCCGAGCGCTACACGGCCGACCGCGCACAACGGCTGT
CGACGTATTCCTCGGTCACGATCGTCGGCCGCTCGGTCGCGCCGTTTCTCGGCGGCACGCTGATCTCGCTCGGAAGCTTC
GGCGCGGTCTACATCGCCTGCGCGATCAGCGGCGTCCTCGCGCTCGTCGTCGGCCTCGCGCTGCGCGACCATGCGCCTCC
TGCCGCGACGAAGATCGAATTGCCGAAGTTCTGGACCAGCCTCGCCACGGTCCTCCGCGATCGCGGCATCATGCTCGTGA
GCCTGGTCGAAGCCGCGCAATACCTCGTGTTCGGCGCGATCGAAGCCTTCCTCGCGCTCTTCGCGTCGTCGCTCGGCATC
CCGGCATGGCAGATCGGCGTGATCCTCGGCGTGCAGCTCGTCAGCATCGTCTTCGCCAAGCCCATCGCGGGCAAAGTGTC
CGACCGGCTCGGACGCCGCCGCGTGATTCTGCCCGGCCTCATGATCGGCGCGGCAAGCGTCGCGCTGCTGCCGTATTTCC
CGAGCTTCGCGGGGCTGTCGGTGCTGAGCCTCGTCTTCGGCCTCGGCTTCGCCACCGTCACCTCGTCGACCGCAGCGCTC
GTCGCGGACCTGACCCGCGACGGCCGCTACGGCTCGTCGATGGGCGTGCTGCGCACCATCATGGACGTCGGCCAGTCGAT
CGGCCCCGTGCTCACGGGCTTCCTGGTCGGCGCCGCGGGCTACCCGAGCGCATTCACGCTATTGGCCGCCATCCTCGTCG
CCGCCGCCGTCCTGCTCGGCATGCTGCTCAAGGGTGTGGATACGAAGGCGTCCGCGGCGCTATAG

Upstream 100 bases:

>100_bases
CGTCTTCATCGCCCTCATCGTCGCGCCGCCGATTTCCGTGTCTGTTCGCATACCCTGCGTTTTCGCACTACAGTCGGGAA
TAACACGCGCCCATCGTCCC

Downstream 100 bases:

>100_bases
TGACCGGGCTATTCGCTCCCAAGGAGACCCCCATGCGCATTACTTCCCTGCTCGCCGCATTCGCATTCGCCGCCGGCCTC
GGCGCGCCTGCCCTCGCCGA

Product: major facilitator superfamily protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MKSARPSFFVLIALVGGLAIFSSTLSKTPVLPLFAQALDATPAEIGWIVMASTIPGILISYPAGALSDQLGKRRVLLASL
VVFATAPFLYLIISTAWQLMAVRFYHGFATAIFGTVASAAIAERYTADRAQRLSTYSSVTIVGRSVAPFLGGTLISLGSF
GAVYIACAISGVLALVVGLALRDHAPPAATKIELPKFWTSLATVLRDRGIMLVSLVEAAQYLVFGAIEAFLALFASSLGI
PAWQIGVILGVQLVSIVFAKPIAGKVSDRLGRRRVILPGLMIGAASVALLPYFPSFAGLSVLSLVFGLGFATVTSSTAAL
VADLTRDGRYGSSMGVLRTIMDVGQSIGPVLTGFLVGAAGYPSAFTLLAAILVAAAVLLGMLLKGVDTKASAAL

Sequences:

>Translated_394_residues
MKSARPSFFVLIALVGGLAIFSSTLSKTPVLPLFAQALDATPAEIGWIVMASTIPGILISYPAGALSDQLGKRRVLLASL
VVFATAPFLYLIISTAWQLMAVRFYHGFATAIFGTVASAAIAERYTADRAQRLSTYSSVTIVGRSVAPFLGGTLISLGSF
GAVYIACAISGVLALVVGLALRDHAPPAATKIELPKFWTSLATVLRDRGIMLVSLVEAAQYLVFGAIEAFLALFASSLGI
PAWQIGVILGVQLVSIVFAKPIAGKVSDRLGRRRVILPGLMIGAASVALLPYFPSFAGLSVLSLVFGLGFATVTSSTAAL
VADLTRDGRYGSSMGVLRTIMDVGQSIGPVLTGFLVGAAGYPSAFTLLAAILVAAAVLLGMLLKGVDTKASAAL
>Mature_394_residues
MKSARPSFFVLIALVGGLAIFSSTLSKTPVLPLFAQALDATPAEIGWIVMASTIPGILISYPAGALSDQLGKRRVLLASL
VVFATAPFLYLIISTAWQLMAVRFYHGFATAIFGTVASAAIAERYTADRAQRLSTYSSVTIVGRSVAPFLGGTLISLGSF
GAVYIACAISGVLALVVGLALRDHAPPAATKIELPKFWTSLATVLRDRGIMLVSLVEAAQYLVFGAIEAFLALFASSLGI
PAWQIGVILGVQLVSIVFAKPIAGKVSDRLGRRRVILPGLMIGAASVALLPYFPSFAGLSVLSLVFGLGFATVTSSTAAL
VADLTRDGRYGSSMGVLRTIMDVGQSIGPVLTGFLVGAAGYPSAFTLLAAILVAAAVLLGMLLKGVDTKASAAL

Specific function: Efflux Pump Driven By The Proton Motive Force. Confers Resistance To Basic, Aromatic And Lipophilic Drugs As Well As Neutral, Non-Aromatic, Zwitterionic Or Nonaromatic Hydrophilic Antibiotics. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily [H]

Homologues:

Organism=Escherichia coli, GI1787065, Length=383, Percent_Identity=26.8929503916449, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 40800; Mature: 40800

Theoretical pI: Translated: 10.47; Mature: 10.47

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSARPSFFVLIALVGGLAIFSSTLSKTPVLPLFAQALDATPAEIGWIVMASTIPGILIS
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHEEEHHHCCCCEEE
YPAGALSDQLGKRRVLLASLVVFATAPFLYLIISTAWQLMAVRFYHGFATAIFGTVASAA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAERYTADRAQRLSTYSSVTIVGRSVAPFLGGTLISLGSFGAVYIACAISGVLALVVGLA
HHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
LRDHAPPAATKIELPKFWTSLATVLRDRGIMLVSLVEAAQYLVFGAIEAFLALFASSLGI
HHCCCCCCCCEEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PAWQIGVILGVQLVSIVFAKPIAGKVSDRLGRRRVILPGLMIGAASVALLPYFPSFAGLS
CHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEHHHHHHHHHHHHHHHHCCCCHHHHH
VLSLVFGLGFATVTSSTAALVADLTRDGRYGSSMGVLRTIMDVGQSIGPVLTGFLVGAAG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
YPSAFTLLAAILVAAAVLLGMLLKGVDTKASAAL
CCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCC
>Mature Secondary Structure
MKSARPSFFVLIALVGGLAIFSSTLSKTPVLPLFAQALDATPAEIGWIVMASTIPGILIS
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHEEEHHHCCCCEEE
YPAGALSDQLGKRRVLLASLVVFATAPFLYLIISTAWQLMAVRFYHGFATAIFGTVASAA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAERYTADRAQRLSTYSSVTIVGRSVAPFLGGTLISLGSFGAVYIACAISGVLALVVGLA
HHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
LRDHAPPAATKIELPKFWTSLATVLRDRGIMLVSLVEAAQYLVFGAIEAFLALFASSLGI
HHCCCCCCCCEEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PAWQIGVILGVQLVSIVFAKPIAGKVSDRLGRRRVILPGLMIGAASVALLPYFPSFAGLS
CHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEHHHHHHHHHHHHHHHHCCCCHHHHH
VLSLVFGLGFATVTSSTAALVADLTRDGRYGSSMGVLRTIMDVGQSIGPVLTGFLVGAAG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
YPSAFTLLAAILVAAAVLLGMLLKGVDTKASAAL
CCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]