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The map label for this gene is rpoN [H]

Identifier: 74316551

GI number: 74316551

Start: 557855

End: 559291

Strand: Reverse

Name: rpoN [H]

Synonym: Tbd_0533

Alternate gene names: 74316551

Gene position: 559291-557855 (Counterclockwise)

Preceding gene: 74316552

Following gene: 74316550

Centisome position: 19.22

GC content: 66.32

Gene sequence:

>1437_bases
ATGAAACACAGCCTCCAGCTCAAGCTCGGTCAGCATCTCACGCTGACGCCTCAGCTGCAGCAGTCGATCCGGCTGCTGCA
GCTGTCGACGCTCGAGTTGAACCAGGAACTCGAGCAGATGCTGCAGGACAATCCGCTGCTCGAACGCGAGGACGACGACG
AGGCGAACCACGCCGCGACCGAAGCACCGGCGCACACCGCGGAAGCCGAAGCGCAAAGCAGCGAAACGCCGCAACTCGAG
CGCGCCGAGGCGGAGCCCGTGACGACGCTCGACGACTCCGACTGGAACGACTACAGCGCCGGCAGTGCCGACGAGGACGA
GGACGGCGATTACGCCCAAGGTTCGGTCGCGGGCGCGACGCTGCGCGAACACCTGCTCAACCAGCTGATCGTGAGCCCGC
TCACCGTGCGCGACCGCGTGCTCGTCGCCGCACTCATCGACGATCTCGACGAAGCCGGTTTCCTCACCCAGCCGCTCGAA
GACATCACGGCGAGCCTGCGCGACGAACTCGAGGACCTCGAGCACGAGGAAGTCGAAACCGCGCTCAAGCACCTGCAGAA
CATGGACCCGACCGGCGTCGGCGCGCGCAATCTGGCCGAATGCCTGACGCTGCAACTGCGCGCCCTTCCCGACGACACGC
CGGTGCGCGCGGCAGCGATGCGGCTCACCACGCACTATCTCGAGCTGCTGGCGGCGCGCGACGTCGGCAAGCTGAAAAAA
ATGCTCGGGATCGACGATCCGACGCTGCGCGCGGCGCGCGCGCTGATCCTCTCGCTCAATCCGCGGCCCGGCTCGGCCTT
CAGCGCCGAACAGACGCACTACGTCATCCCCGACGTCTACGTGCGCAAGGTCAAGGGCATGTGGATCGCGAGCCTGAACG
CCGACGCGATGCCCAAGCTGCGGGTCAACCGCGTCTACGCCGACATCCTGGCGCGACATCGCGAATCGGGCGGCAGCCAG
CTCGCGAGCCAGCTACAGGAGGCGCGCTGGCTGATCAAGAACGTCCAGCAACGCTTCGACACGATCCTGCGCGTCTCTCA
GGCGATCGTCGATCGGCAGAAGCATTTCTTCGAGCACGGCGAGGTCGCGATGCGGCCGCTGGTGCTGCGGGAGATCGCCG
ACGCGGTCGATCTGCACGAATCGACGATCTCGCGCGTGACGACGCAGAAATTCATGATGACCCCGCGCGGCCTCTACGAA
CTCAAGTACTTCTTCGGCAGCCACGTCGCGACCGACAACGGCGGCGCGTGCTCGTCGACCGCGATCCGCGCGCTGATCAA
GCAGTTGATCGCCGCCGAAAGCGGCAAGAAGCCGTTGTCCGACGGTCAGCTCGCCGACGTCCTCGGCCAGCAGGGCATCG
TCGTCGCGCGCCGAACGGTAGCCAAATACCGCGAGTCGCTGCAGATCCCTCCGGCCAACATGCGCCGCGCCCTCTGA

Upstream 100 bases:

>100_bases
AAGTTCTACCTCGGCGAAAATTTCCGTCTCTGATGAGACGAAACGGGAAACGCGCTACACTCCGTTCAAGTCGGCACGAT
TTTTGCCGTAGTCAGAATCC

Downstream 100 bases:

>100_bases
GGCGTCACGCGCCCCTCCGAGGCCGCCCGGCGGCAATGGAGCGAGGCGCTTGAATGTCTCCGGCTTTCGTCTCAGAATGA
AATCGAGCGAGGGTTTGCTC

Product: RNA polymerase factor sigma-54

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MKHSLQLKLGQHLTLTPQLQQSIRLLQLSTLELNQELEQMLQDNPLLEREDDDEANHAATEAPAHTAEAEAQSSETPQLE
RAEAEPVTTLDDSDWNDYSAGSADEDEDGDYAQGSVAGATLREHLLNQLIVSPLTVRDRVLVAALIDDLDEAGFLTQPLE
DITASLRDELEDLEHEEVETALKHLQNMDPTGVGARNLAECLTLQLRALPDDTPVRAAAMRLTTHYLELLAARDVGKLKK
MLGIDDPTLRAARALILSLNPRPGSAFSAEQTHYVIPDVYVRKVKGMWIASLNADAMPKLRVNRVYADILARHRESGGSQ
LASQLQEARWLIKNVQQRFDTILRVSQAIVDRQKHFFEHGEVAMRPLVLREIADAVDLHESTISRVTTQKFMMTPRGLYE
LKYFFGSHVATDNGGACSSTAIRALIKQLIAAESGKKPLSDGQLADVLGQQGIVVARRTVAKYRESLQIPPANMRRAL

Sequences:

>Translated_478_residues
MKHSLQLKLGQHLTLTPQLQQSIRLLQLSTLELNQELEQMLQDNPLLEREDDDEANHAATEAPAHTAEAEAQSSETPQLE
RAEAEPVTTLDDSDWNDYSAGSADEDEDGDYAQGSVAGATLREHLLNQLIVSPLTVRDRVLVAALIDDLDEAGFLTQPLE
DITASLRDELEDLEHEEVETALKHLQNMDPTGVGARNLAECLTLQLRALPDDTPVRAAAMRLTTHYLELLAARDVGKLKK
MLGIDDPTLRAARALILSLNPRPGSAFSAEQTHYVIPDVYVRKVKGMWIASLNADAMPKLRVNRVYADILARHRESGGSQ
LASQLQEARWLIKNVQQRFDTILRVSQAIVDRQKHFFEHGEVAMRPLVLREIADAVDLHESTISRVTTQKFMMTPRGLYE
LKYFFGSHVATDNGGACSSTAIRALIKQLIAAESGKKPLSDGQLADVLGQQGIVVARRTVAKYRESLQIPPANMRRAL
>Mature_478_residues
MKHSLQLKLGQHLTLTPQLQQSIRLLQLSTLELNQELEQMLQDNPLLEREDDDEANHAATEAPAHTAEAEAQSSETPQLE
RAEAEPVTTLDDSDWNDYSAGSADEDEDGDYAQGSVAGATLREHLLNQLIVSPLTVRDRVLVAALIDDLDEAGFLTQPLE
DITASLRDELEDLEHEEVETALKHLQNMDPTGVGARNLAECLTLQLRALPDDTPVRAAAMRLTTHYLELLAARDVGKLKK
MLGIDDPTLRAARALILSLNPRPGSAFSAEQTHYVIPDVYVRKVKGMWIASLNADAMPKLRVNRVYADILARHRESGGSQ
LASQLQEARWLIKNVQQRFDTILRVSQAIVDRQKHFFEHGEVAMRPLVLREIADAVDLHESTISRVTTQKFMMTPRGLYE
LKYFFGSHVATDNGGACSSTAIRALIKQLIAAESGKKPLSDGQLADVLGQQGIVVARRTVAKYRESLQIPPANMRRAL

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=494, Percent_Identity=47.7732793522267, Blast_Score=428, Evalue=1e-121,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 53026; Mature: 53026

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHSLQLKLGQHLTLTPQLQQSIRLLQLSTLELNQELEQMLQDNPLLEREDDDEANHAAT
CCCCEEEECCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
EAPAHTAEAEAQSSETPQLERAEAEPVTTLDDSDWNDYSAGSADEDEDGDYAQGSVAGAT
CCCCHHHHHHHCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
LREHLLNQLIVSPLTVRDRVLVAALIDDLDEAGFLTQPLEDITASLRDELEDLEHEEVET
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHCCHHHHHH
ALKHLQNMDPTGVGARNLAECLTLQLRALPDDTPVRAAAMRLTTHYLELLAARDVGKLKK
HHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
MLGIDDPTLRAARALILSLNPRPGSAFSAEQTHYVIPDVYVRKVKGMWIASLNADAMPKL
HHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCHHHHHHHCCCEEEECCCCCCCHH
RVNRVYADILARHRESGGSQLASQLQEARWLIKNVQQRFDTILRVSQAIVDRQKHFFEHG
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EVAMRPLVLREIADAVDLHESTISRVTTQKFMMTPRGLYELKYFFGSHVATDNGGACSST
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEECCCCCCHHHH
AIRALIKQLIAAESGKKPLSDGQLADVLGQQGIVVARRTVAKYRESLQIPPANMRRAL
HHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHCCC
>Mature Secondary Structure
MKHSLQLKLGQHLTLTPQLQQSIRLLQLSTLELNQELEQMLQDNPLLEREDDDEANHAAT
CCCCEEEECCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
EAPAHTAEAEAQSSETPQLERAEAEPVTTLDDSDWNDYSAGSADEDEDGDYAQGSVAGAT
CCCCHHHHHHHCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
LREHLLNQLIVSPLTVRDRVLVAALIDDLDEAGFLTQPLEDITASLRDELEDLEHEEVET
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHCCHHHHHH
ALKHLQNMDPTGVGARNLAECLTLQLRALPDDTPVRAAAMRLTTHYLELLAARDVGKLKK
HHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
MLGIDDPTLRAARALILSLNPRPGSAFSAEQTHYVIPDVYVRKVKGMWIASLNADAMPKL
HHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCHHHHHHHCCCEEEECCCCCCCHH
RVNRVYADILARHRESGGSQLASQLQEARWLIKNVQQRFDTILRVSQAIVDRQKHFFEHG
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EVAMRPLVLREIADAVDLHESTISRVTTQKFMMTPRGLYELKYFFGSHVATDNGGACSST
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEECCCCCCHHHH
AIRALIKQLIAAESGKKPLSDGQLADVLGQQGIVVARRTVAKYRESLQIPPANMRRAL
HHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1417413 [H]