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The map label for this gene is ptsN [H]

Identifier: 74316549

GI number: 74316549

Start: 556823

End: 557278

Strand: Reverse

Name: ptsN [H]

Synonym: Tbd_0531

Alternate gene names: 74316549

Gene position: 557278-556823 (Counterclockwise)

Preceding gene: 74316550

Following gene: 74316548

Centisome position: 19.15

GC content: 63.38

Gene sequence:

>456_bases
ATGAACCTAATCGCCCCCCTTCTCACTCCTGCCACCACGCTTCTGGACGTCTCGTTCAGCAGCAAGAAAAAGCTGTTCGA
GCATGCGGCCGAACTGTTCGCGCAGACGCACGGGCTCAAGGCGTCCGACATATTCAGCAGCCTGTTCGAGCGTGAACGGC
TCGGCTCCACGGCGCTCGGCTACGGCATCGCGATTCCGCACGGCCGCATCAAGGGGCTTCAGGACGCGGCCGGCGCGTTC
TACCGCCTGAGCACGCCGCTCGAGTTCGACGCGCCCGACAACCAACCGGTCTCGCTGTGTTTCGTTTTGCTCGTCCCGAA
GGACGCGAACGAAGCGCATCTGCAGATCCTCGGCGAACTCGCGCAGTTGTTCGGTGACGAAGGCATGCGTGCCCGCATGT
TCGAAGCCGCCTCGCCTTCGGATCTGATCGCACTGGTCGCGGCCTGGTCCGGTTAG

Upstream 100 bases:

>100_bases
GAAAACCCGGCCTGACGGCCCGGCCGAATGGCCGCGCCCGGGGGTTGCTCCGGGCGTATAATCGCGATCGCGGGCCTTAC
ATCCGGGCCTCGCAACAGGC

Downstream 100 bases:

>100_bases
CGGCGCATGGAGGAAGGCGCGCGCGTTTCGGTCGAGACCCTGTTCCGCGACATGGCGGGACAAGTCGACCTGCAATGGGC
CGCGGCCCAGCAGGGCGGCG

Product: PTS IIA-like nitrogen-regulatory protein PtsN

Products: protein histidine; sugar phosphate

Alternate protein names: Enzyme IIA-NTR; Phosphotransferase enzyme IIA component; PTS system EIIA component [H]

Number of amino acids: Translated: 151; Mature: 151

Protein sequence:

>151_residues
MNLIAPLLTPATTLLDVSFSSKKKLFEHAAELFAQTHGLKASDIFSSLFERERLGSTALGYGIAIPHGRIKGLQDAAGAF
YRLSTPLEFDAPDNQPVSLCFVLLVPKDANEAHLQILGELAQLFGDEGMRARMFEAASPSDLIALVAAWSG

Sequences:

>Translated_151_residues
MNLIAPLLTPATTLLDVSFSSKKKLFEHAAELFAQTHGLKASDIFSSLFERERLGSTALGYGIAIPHGRIKGLQDAAGAF
YRLSTPLEFDAPDNQPVSLCFVLLVPKDANEAHLQILGELAQLFGDEGMRARMFEAASPSDLIALVAAWSG
>Mature_151_residues
MNLIAPLLTPATTLLDVSFSSKKKLFEHAAELFAQTHGLKASDIFSSLFERERLGSTALGYGIAIPHGRIKGLQDAAGAF
YRLSTPLEFDAPDNQPVSLCFVLLVPKDANEAHLQILGELAQLFGDEGMRARMFEAASPSDLIALVAAWSG

Specific function: Seems to have a role in regulating nitrogen assimilation [H]

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1789597, Length=149, Percent_Identity=37.5838926174497, Blast_Score=102, Evalue=1e-23,
Organism=Escherichia coli, GI1786951, Length=133, Percent_Identity=31.5789473684211, Blast_Score=75, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR006320 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 16259; Mature: 16259

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLIAPLLTPATTLLDVSFSSKKKLFEHAAELFAQTHGLKASDIFSSLFERERLGSTALG
CCCCHHHHCCHHHHEECCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHC
YGIAIPHGRIKGLQDAAGAFYRLSTPLEFDAPDNQPVSLCFVLLVPKDANEAHLQILGEL
CEEECCCCHHCCCHHHCCCEEECCCCEECCCCCCCCEEEEEEEEECCCCCHHHHHHHHHH
AQLFGDEGMRARMFEAASPSDLIALVAAWSG
HHHHCCCCCHHHHHCCCCCHHHHHHEEECCC
>Mature Secondary Structure
MNLIAPLLTPATTLLDVSFSSKKKLFEHAAELFAQTHGLKASDIFSSLFERERLGSTALG
CCCCHHHHCCHHHHEECCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHC
YGIAIPHGRIKGLQDAAGAFYRLSTPLEFDAPDNQPVSLCFVLLVPKDANEAHLQILGEL
CEEECCCCHHCCCHHHCCCEEECCCCEECCCCCCCCEEEEEEEEECCCCCHHHHHHHHHH
AQLFGDEGMRARMFEAASPSDLIALVAAWSG
HHHHCCCCCHHHHHCCCCCHHHHHHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]