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The map label for this gene is ykuT [H]

Identifier: 74316517

GI number: 74316517

Start: 519183

End: 520148

Strand: Direct

Name: ykuT [H]

Synonym: Tbd_0499

Alternate gene names: 74316517

Gene position: 519183-520148 (Clockwise)

Preceding gene: 74316505

Following gene: 74316519

Centisome position: 17.84

GC content: 67.18

Gene sequence:

>966_bases
ATGGATGCGCTAACCCAGTATTTCCCCGACCTCGAGACCTGGCGCGGGCCGCTCGTGAGCGTCCTGCACGTCGTGCTGAT
ACTGGTCCTGGCCTGGCTCGCGCTGCGACTGTCGCGCAGGGGGCTCGCCCGTCTGCGCATGCGCCTGCAGCAGGATCTCG
ACGACTCCGAGCGCATCAAGCGCCTCAATACGCTCGAGCAGGTCTTCCGCTACGTGCTTGCGGTGCTGATCATCCTCGTC
GCCGGCATGCTCGTGCTGTCGGAACTCGGCATCTCGATCGCGCCGGTGCTCGCCGCCGCCGGGGTCGTCGGCATCGCGGT
CGGCTTCGGCGCGCAGAGCCTGGTCAAGGATTACTTCACCGGCCTGTTCCTGCTGCTCGAAAACCAGGTGCGCCAGGGCG
ACGTCGTCGAAGTCGGCGGCAAGGGCGGGCTGGTCGAGGAGATGACGCTGCGCTACATCCGGCTGCGCGATTACGAGGGG
AGCGTGCATTACGTGCCCAACGGCATCATCGACACCGTCACCAACCGCAGCCGCGGCTTTGCCTTCGCGCTGATCGACGT
CAGTGTCGCCTACCGCGAGGACGTCGACGCCGTGTGTGCGGTGATGCGCGAGGTCGCGGCCGACCTGCGTGCCGACCCGG
AACTGTCGAGCAAGATCGTCGAGGACATCGAGATCGCAGGCGTCGAACAGTGGGCCGACTCGGCGGTCGTGATCCGGTGC
CGCTTCAAGACCCTGCCGCTCGAGCAGTGGAACGTGCGCCGTGCGTATCTGCAGCGGCTCAAGAAGGCCTTCGACGCGGC
GGACATCGAGATTCCGTTTCCGCATCTCACGCTTTACCAGGGTCAGAACAAGGACGGCAGCGCGCCGCCGCTGCGCCTGG
AGCGGGCGCGCGGCGGGCGGACGGCGGAAAATCCGCATGCTGGCGCGGCCAGGCTGACTTCGGTCAATCCCGGCACCGGG
CGCTGA

Upstream 100 bases:

>100_bases
GCCGTAAAACTACCGTGGATATTTTTCTGGAGCCATTGATGAGAACGTCGACTCAAGCGATCGCAGCGTTTCCGGTCGCG
GCCGCGCCGAGGAGTGCGAA

Downstream 100 bases:

>100_bases
GCTCGCTGCCGCCGGCGCGGCGGGCAGGCCGGTAACAGGGTGAATCCTGGAAAGATTTTGGGCATCCTCCCCGGGGGGAG
GTCTACGTCCGATCGTCGCG

Product: mechanosensitive ion channel

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MDALTQYFPDLETWRGPLVSVLHVVLILVLAWLALRLSRRGLARLRMRLQQDLDDSERIKRLNTLEQVFRYVLAVLIILV
AGMLVLSELGISIAPVLAAAGVVGIAVGFGAQSLVKDYFTGLFLLLENQVRQGDVVEVGGKGGLVEEMTLRYIRLRDYEG
SVHYVPNGIIDTVTNRSRGFAFALIDVSVAYREDVDAVCAVMREVAADLRADPELSSKIVEDIEIAGVEQWADSAVVIRC
RFKTLPLEQWNVRRAYLQRLKKAFDAADIEIPFPHLTLYQGQNKDGSAPPLRLERARGGRTAENPHAGAARLTSVNPGTG
R

Sequences:

>Translated_321_residues
MDALTQYFPDLETWRGPLVSVLHVVLILVLAWLALRLSRRGLARLRMRLQQDLDDSERIKRLNTLEQVFRYVLAVLIILV
AGMLVLSELGISIAPVLAAAGVVGIAVGFGAQSLVKDYFTGLFLLLENQVRQGDVVEVGGKGGLVEEMTLRYIRLRDYEG
SVHYVPNGIIDTVTNRSRGFAFALIDVSVAYREDVDAVCAVMREVAADLRADPELSSKIVEDIEIAGVEQWADSAVVIRC
RFKTLPLEQWNVRRAYLQRLKKAFDAADIEIPFPHLTLYQGQNKDGSAPPLRLERARGGRTAENPHAGAARLTSVNPGTG
R
>Mature_321_residues
MDALTQYFPDLETWRGPLVSVLHVVLILVLAWLALRLSRRGLARLRMRLQQDLDDSERIKRLNTLEQVFRYVLAVLIILV
AGMLVLSELGISIAPVLAAAGVVGIAVGFGAQSLVKDYFTGLFLLLENQVRQGDVVEVGGKGGLVEEMTLRYIRLRDYEG
SVHYVPNGIIDTVTNRSRGFAFALIDVSVAYREDVDAVCAVMREVAADLRADPELSSKIVEDIEIAGVEQWADSAVVIRC
RFKTLPLEQWNVRRAYLQRLKKAFDAADIEIPFPHLTLYQGQNKDGSAPPLRLERARGGRTAENPHAGAARLTSVNPGTG
R

Specific function: May play a role in resistance to osmotic downshock [H]

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI87081787, Length=229, Percent_Identity=37.5545851528384, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1789291, Length=267, Percent_Identity=23.5955056179775, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1786670, Length=143, Percent_Identity=28.6713286713287, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1787591, Length=235, Percent_Identity=22.5531914893617, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR011014 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 35545; Mature: 35545

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDALTQYFPDLETWRGPLVSVLHVVLILVLAWLALRLSRRGLARLRMRLQQDLDDSERIK
CCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
RLNTLEQVFRYVLAVLIILVAGMLVLSELGISIAPVLAAAGVVGIAVGFGAQSLVKDYFT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
GLFLLLENQVRQGDVVEVGGKGGLVEEMTLRYIRLRDYEGSVHYVPNGIIDTVTNRSRGF
HHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHEECCCCCEEECCCCHHHHHCCCCCCE
AFALIDVSVAYREDVDAVCAVMREVAADLRADPELSSKIVEDIEIAGVEQWADSAVVIRC
EEEEEEEHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCHHHHCCCEEEEEE
RFKTLPLEQWNVRRAYLQRLKKAFDAADIEIPFPHLTLYQGQNKDGSAPPLRLERARGGR
ECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCHHHCCCCC
TAENPHAGAARLTSVNPGTGR
CCCCCCCCCCEEEECCCCCCC
>Mature Secondary Structure
MDALTQYFPDLETWRGPLVSVLHVVLILVLAWLALRLSRRGLARLRMRLQQDLDDSERIK
CCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
RLNTLEQVFRYVLAVLIILVAGMLVLSELGISIAPVLAAAGVVGIAVGFGAQSLVKDYFT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
GLFLLLENQVRQGDVVEVGGKGGLVEEMTLRYIRLRDYEGSVHYVPNGIIDTVTNRSRGF
HHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHEECCCCCEEECCCCHHHHHCCCCCCE
AFALIDVSVAYREDVDAVCAVMREVAADLRADPELSSKIVEDIEIAGVEQWADSAVVIRC
EEEEEEEHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCHHHHCCCEEEEEE
RFKTLPLEQWNVRRAYLQRLKKAFDAADIEIPFPHLTLYQGQNKDGSAPPLRLERARGGR
ECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCHHHCCCCC
TAENPHAGAARLTSVNPGTGR
CCCCCCCCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]