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The map label for this gene is phaD [H]

Identifier: 74316513

GI number: 74316513

Start: 512543

End: 514057

Strand: Reverse

Name: phaD [H]

Synonym: Tbd_0495

Alternate gene names: 74316513

Gene position: 514057-512543 (Counterclockwise)

Preceding gene: 74316514

Following gene: 74316512

Centisome position: 17.67

GC content: 71.29

Gene sequence:

>1515_bases
ATGAGCGGCCTGAGCACGCACCTGCCGATCCTGCCCGTGCTGCTGCCGCTGGTCGCCGGCATCGTGCTGCTGGCGCTGCG
CGGCGCGCCGCTCGTGCTCCAGCGCGCCGCGAGCCTGGTCGCGACCGCGGCCCTGGTGCCGCTCGCGTTCGCGCTGCTGG
GCACGGCCGGCGAGGGCACCCAGCTCGTCTACAAGCTCGGCGACTGGGACGCGCCCTTCGGCATCGTGCTCGTCGCCGAC
CGCCTCGCCGCCTGGATGCTGATGGCCACCGCGCTGGTCGCGCTGTTCGCCCTGCTGCAGGCGCTCGCCGGCACCGACGT
CGAGGGCCGCCACTTCCATGTGCTCTACCAGCTGCAGCTCTTTGGCCTGAACGGCGCCTTCCTCACCGGCGACCTCTTCA
ACCTGTTCGTGTTCTTCGAGATCCTGCTGCTCGCCTCCTACGGCCTGCTGCTGCACGGCGGCGGCAGGCTGCGCGTCAAG
GCGGGTCTCCATTTCGTCGTCATCAACCTGGTCGGCTCGACGCTCTTTCTGTTCGCCGTCGGCACGCTCTACGGCGTCGC
CGGCACGCTCAACATGGCCGACCTGGCGGTCAAGCTCGCGCGCACGGTTCCCGAGAATCTGGCGCTCGTCGAGGCAGCCG
GGCTCATGCTGTTCGGCGTCTTCGCGCTCAAGGCCGCACTGCTGCCGCTGCACCTGTGGCTACCCGCCGCCTACGCACAC
ACCAGCGCGCCGGTCGCGGCGCTGTTCGCGATCATGACCAAGGTCGGCGCCTACAGCATCCTGCGCGTCTACAGCCTGAT
GTTCGGTGCCGAGGCGGGGGCGGTCGCCAACCTGCTCGCCGACTGGCTGATGCCGCTCGCGCTGGCGACGCTCGTCGCCG
GCACGCTCGGCGCGGTCGCGAGCACGAGCCTGCGCCAGCAGATCGCCTATCTGGTCATCGCCTCGGTCGGCACCCTGCTC
GCCGCCTTCGCCCTCGACAGCGCCGAAGGTATCGCGGCGGGCCTCTACTACCTGCCTCATACGACCCTCGGCACGGCGGC
CTTCTTCCTGCTCGCGGCGCCGATCGCGGGCGCGCGCGGCACGCTCGGCGACCGCTTCGACTCGGGGGCCGCGATGCCGG
GCGCGCGCGTGCTCGGCGGTCTCTTCTTCGTCGCTGCGATCGCGCTCGCGGGCCTGCCGCCGCTGTCGGGCTTTCTCGGC
AAATTCATGCTGCTGCGCGCGGCACTCGGCTCGCCGTGGCTGGCGTGGGTCTGGGCGATCGTGCTGACGACGAGCCTCTT
CGCGGTGATCGCCCTGGCGCGCAGCGGCAGCCTGCTGTTCTACCGCACGCACTCGACCCCCGAGGTGCTCTCCGCGCCCC
GCCCCATCGAATGGCTGCCCGCGGCCGGCCTGCTGCTGCTCGTACTCGGCATGGTGATCTGGGCCGGAACGCTCGCCGAC
TACAGCGCAGCGACGGCGGCGCAACTGCTCGCCCCCCAGACCTACATCGACGCCGTGCTCGGAGACCGGCCATGA

Upstream 100 bases:

>100_bases
TCGGCATGACGGCCTTCGTCATCGTGCTCGCGCTGAAGGCGCGCGCCGAATTCGGCAACGACCATGTCGACGGCACCCAC
AGCGGGGAGGACACGGCGCG

Downstream 100 bases:

>100_bases
GGCGCCTGCTGCCGCACCCGCTGCTGACACTCGCGCTGGCCCTGCTCTGGTTGCTGCTGGTCAACGACTTCTCGGCCGGC
CATGCGGTGCTCGGCGCGCT

Product: putative monovalent cation/H+ antiporter subunit D

Products: NAD+; ubiquinol [C]

Alternate protein names: pH adaptation potassium efflux system protein D; Pha system subunit D [H]

Number of amino acids: Translated: 504; Mature: 503

Protein sequence:

>504_residues
MSGLSTHLPILPVLLPLVAGIVLLALRGAPLVLQRAASLVATAALVPLAFALLGTAGEGTQLVYKLGDWDAPFGIVLVAD
RLAAWMLMATALVALFALLQALAGTDVEGRHFHVLYQLQLFGLNGAFLTGDLFNLFVFFEILLLASYGLLLHGGGRLRVK
AGLHFVVINLVGSTLFLFAVGTLYGVAGTLNMADLAVKLARTVPENLALVEAAGLMLFGVFALKAALLPLHLWLPAAYAH
TSAPVAALFAIMTKVGAYSILRVYSLMFGAEAGAVANLLADWLMPLALATLVAGTLGAVASTSLRQQIAYLVIASVGTLL
AAFALDSAEGIAAGLYYLPHTTLGTAAFFLLAAPIAGARGTLGDRFDSGAAMPGARVLGGLFFVAAIALAGLPPLSGFLG
KFMLLRAALGSPWLAWVWAIVLTTSLFAVIALARSGSLLFYRTHSTPEVLSAPRPIEWLPAAGLLLLVLGMVIWAGTLAD
YSAATAAQLLAPQTYIDAVLGDRP

Sequences:

>Translated_504_residues
MSGLSTHLPILPVLLPLVAGIVLLALRGAPLVLQRAASLVATAALVPLAFALLGTAGEGTQLVYKLGDWDAPFGIVLVAD
RLAAWMLMATALVALFALLQALAGTDVEGRHFHVLYQLQLFGLNGAFLTGDLFNLFVFFEILLLASYGLLLHGGGRLRVK
AGLHFVVINLVGSTLFLFAVGTLYGVAGTLNMADLAVKLARTVPENLALVEAAGLMLFGVFALKAALLPLHLWLPAAYAH
TSAPVAALFAIMTKVGAYSILRVYSLMFGAEAGAVANLLADWLMPLALATLVAGTLGAVASTSLRQQIAYLVIASVGTLL
AAFALDSAEGIAAGLYYLPHTTLGTAAFFLLAAPIAGARGTLGDRFDSGAAMPGARVLGGLFFVAAIALAGLPPLSGFLG
KFMLLRAALGSPWLAWVWAIVLTTSLFAVIALARSGSLLFYRTHSTPEVLSAPRPIEWLPAAGLLLLVLGMVIWAGTLAD
YSAATAAQLLAPQTYIDAVLGDRP
>Mature_503_residues
SGLSTHLPILPVLLPLVAGIVLLALRGAPLVLQRAASLVATAALVPLAFALLGTAGEGTQLVYKLGDWDAPFGIVLVADR
LAAWMLMATALVALFALLQALAGTDVEGRHFHVLYQLQLFGLNGAFLTGDLFNLFVFFEILLLASYGLLLHGGGRLRVKA
GLHFVVINLVGSTLFLFAVGTLYGVAGTLNMADLAVKLARTVPENLALVEAAGLMLFGVFALKAALLPLHLWLPAAYAHT
SAPVAALFAIMTKVGAYSILRVYSLMFGAEAGAVANLLADWLMPLALATLVAGTLGAVASTSLRQQIAYLVIASVGTLLA
AFALDSAEGIAAGLYYLPHTTLGTAAFFLLAAPIAGARGTLGDRFDSGAAMPGARVLGGLFFVAAIALAGLPPLSGFLGK
FMLLRAALGSPWLAWVWAIVLTTSLFAVIALARSGSLLFYRTHSTPEVLSAPRPIEWLPAAGLLLLVLGMVIWAGTLADY
SAATAAQLLAPQTYIDAVLGDRP

Specific function: Part of a K(+) efflux system which is required for the adaptation of R.meliloti to alkaline pH as well as for the infection process during symbiotic nodule development [H]

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit D family [H]

Homologues:

Organism=Escherichia coli, GI1788613, Length=417, Percent_Identity=25.8992805755396, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI145693160, Length=356, Percent_Identity=26.4044943820225, Blast_Score=79, Evalue=9e-16,
Organism=Escherichia coli, GI1788827, Length=293, Percent_Identity=25.938566552901, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1788831, Length=308, Percent_Identity=24.3506493506494, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004775
- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.6.99.5 [C]

Molecular weight: Translated: 52386; Mature: 52255

Theoretical pI: Translated: 8.67; Mature: 8.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGLSTHLPILPVLLPLVAGIVLLALRGAPLVLQRAASLVATAALVPLAFALLGTAGEGT
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
QLVYKLGDWDAPFGIVLVADRLAAWMLMATALVALFALLQALAGTDVEGRHFHVLYQLQL
EEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEE
FGLNGAFLTGDLFNLFVFFEILLLASYGLLLHGGGRLRVKAGLHFVVINLVGSTLFLFAV
ECCCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCEEEEECCCHHHHHHHHHHHHHHHHH
GTLYGVAGTLNMADLAVKLARTVPENLALVEAAGLMLFGVFALKAALLPLHLWLPAAYAH
HHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TSAPVAALFAIMTKVGAYSILRVYSLMFGAEAGAVANLLADWLMPLALATLVAGTLGAVA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
STSLRQQIAYLVIASVGTLLAAFALDSAEGIAAGLYYLPHTTLGTAAFFLLAAPIAGARG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCC
TLGDRFDSGAAMPGARVLGGLFFVAAIALAGLPPLSGFLGKFMLLRAALGSPWLAWVWAI
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHH
VLTTSLFAVIALARSGSLLFYRTHSTPEVLSAPRPIEWLPAAGLLLLVLGMVIWAGTLAD
HHHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
YSAATAAQLLAPQTYIDAVLGDRP
HHHHHHHHHHCCHHHHHHHHCCCC
>Mature Secondary Structure 
SGLSTHLPILPVLLPLVAGIVLLALRGAPLVLQRAASLVATAALVPLAFALLGTAGEGT
CCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
QLVYKLGDWDAPFGIVLVADRLAAWMLMATALVALFALLQALAGTDVEGRHFHVLYQLQL
EEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEE
FGLNGAFLTGDLFNLFVFFEILLLASYGLLLHGGGRLRVKAGLHFVVINLVGSTLFLFAV
ECCCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCEEEEECCCHHHHHHHHHHHHHHHHH
GTLYGVAGTLNMADLAVKLARTVPENLALVEAAGLMLFGVFALKAALLPLHLWLPAAYAH
HHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TSAPVAALFAIMTKVGAYSILRVYSLMFGAEAGAVANLLADWLMPLALATLVAGTLGAVA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
STSLRQQIAYLVIASVGTLLAAFALDSAEGIAAGLYYLPHTTLGTAAFFLLAAPIAGARG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCC
TLGDRFDSGAAMPGARVLGGLFFVAAIALAGLPPLSGFLGKFMLLRAALGSPWLAWVWAI
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHH
VLTTSLFAVIALARSGSLLFYRTHSTPEVLSAPRPIEWLPAAGLLLLVLGMVIWAGTLAD
HHHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
YSAATAAQLLAPQTYIDAVLGDRP
HHHHHHHHHHCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; ubiquinone [C]

Specific reaction: NADH + ubiquinone = NAD+ + ubiquinol [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9680201; 11481430 [H]