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The map label for this gene is aspS

Identifier: 74316460

GI number: 74316460

Start: 462569

End: 464362

Strand: Direct

Name: aspS

Synonym: Tbd_0442

Alternate gene names: 74316460

Gene position: 462569-464362 (Clockwise)

Preceding gene: 74316459

Following gene: 74316461

Centisome position: 15.9

GC content: 64.72

Gene sequence:

>1794_bases
ATGCGTACTCACTACTGCGGCGCGGTCACGGCCGCCGACGTCGGCAACACGGTCACTTTGTGCGGATGGGCGCACCGGCG
ACGCGATCACGGCGGCGTGATCTTCATCGACCTGCGCGACCGTGAGGGCATGGTTCAGGTCGTGGTCGACCCCGATACGC
CGGCGGCATTCAAGCTCGCCGAGGACGTGCGCGCCGAATTCGTGCTGAAGATCGAAGGCAAGGTTCGTCCGCGTCCGGCA
GGGACGGAGAATCCGCACCTGCCGACCGGGATGGTCGAGGTGTTGACGCTCGATCTGGACGTGCTGAACCCCTCGCTGAC
GCCGCCGTTCCAGATCGACGACGAGACCATCAACGAGAACATCCGACTCCAGTACCGTTACCTCGACCTGCGCCGCGAGC
CGATGCAGAAGAACCTCAAGCTGCGCTATCGCGTGTCGAAGATCATGCGCGACTACCTCGATACGCACGGCTTCATGGAG
GTCGAGACGCCGATGCTGACGCGCTCGACGCCCGAAGGCGCGCGCGACTATCTGGTGCCGAGCCGGGTGCACGACGGCAT
GTTCTACGCGCTGCCGCAGAGCCCGCAGCTCTTCAAGCAGCTTCTGATGGTCTCCGGCGTCGACCGCTACTTCCAGATCA
CGAAGTGCTTCCGCGACGAGGACTTGCGCGCCGACCGCCAGCCCGAGTTCACGCAAGTGGATTTGGAGACCTCGTTCATG
GGCGAGGAAGCGATCATGGGCCTCGTCGAGGGCATGATTCGCGACATGATGCAGAAGGCCCAGGGCATCGAACTTCCCGC
GGCCTTCCCGCGCATGAGCTACGCCGAGGCGATGAATCGATACGGGTCGGACAAGCCCGATCTGCGCGTCACGCTCGAAA
TCGTCGACGTCGGCGACGTCATGAGGGACGTCGCGTTCAAGGTCTTCGCCACACCTGCCAATGATCCGAAAGGTCGTGTC
GCGGCGCTGCGGATTCCGGGCGGCGCGACGCTCTCGCGCAGCGAAATCGACGGCTACACCGAGTTCGTCAAGATTTACGG
CGCCAAGGGGCTGGCCTACATCAAGGTCAACGACGTCGGCCAGTTGAACGAAGCGGGCCTGCAGTCGCCGATCGTCAAGA
ACCTCTCAGAAGCCGCCTTGCACGCGGTGATGGAGCGGACCGGCGCGCAGAACGGTGACCTGATCTTCTTCGGCGCCGAC
CGCGCCAAGGTGGTCAACGACGCGCTCGGCGCGCTGCGGCTCAAGATCGGCCACGAGAAAGGTCACGTCGACGGTCGCGC
CTGGGCGCCGCTCTGGGTCGTCGACTTCCCGATGTTCGAATACAACGAGGACGAGAACCGCTGGGACGCGCTGCACCATC
CCTTCACCGCGCCCAAGGACGGCCACGAGGACTGGCTCGCCACCGACCCCGGCAAGTGTCTGTCCAAGGCCTACGACATG
GTGCTGAACGGTTGGGAAGTCGGCGGCGGCTCGGTGCGTATCCACCGCGAGAAGGTGCAGGAAAAGGTCTTCGCCGCCCT
CAACATCGGCGAGGAAGAGCGCCGCGAGAAATTCGGCTTCCTGCTCGACGCCCTGCAATACGGCGCGCCGCCCCATGGCG
GCCTCGCATTCGGGCTCGATAGATTGGTCACGCTGATGGCCGGGGCCGAGTCGATCCGCGACGTCATCGCCTTCCCCAAG
ACGCAGCGCGCCTCGTGCCTGATGACCAACGCGCCCAACGTCGTCGACGAAAAGCAGCTGCGCGAGTTGCACATCCGCCT
GCGCAATCCGATCGCCGCCGACAAGGCAGCCTGA

Upstream 100 bases:

>100_bases
TGATCGAACTGGCCGTGCCGGTCGACCGCGCGCTCAAATATATCGTCTCGATGGGCGTCGTTTCCGGCGACCCCCGGGGT
AGCCAATAGGAAAAACGAAC

Downstream 100 bases:

>100_bases
GCCGAGATGAAATTCGCCGATACCCTGAACACCCTGCCAGAGTTCACCGGCGAAGCGGTGGTCCTGAGCGACGCGGCCGG
CGCCGAGCTCGGTCGGATCG

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 597; Mature: 597

Protein sequence:

>597_residues
MRTHYCGAVTAADVGNTVTLCGWAHRRRDHGGVIFIDLRDREGMVQVVVDPDTPAAFKLAEDVRAEFVLKIEGKVRPRPA
GTENPHLPTGMVEVLTLDLDVLNPSLTPPFQIDDETINENIRLQYRYLDLRREPMQKNLKLRYRVSKIMRDYLDTHGFME
VETPMLTRSTPEGARDYLVPSRVHDGMFYALPQSPQLFKQLLMVSGVDRYFQITKCFRDEDLRADRQPEFTQVDLETSFM
GEEAIMGLVEGMIRDMMQKAQGIELPAAFPRMSYAEAMNRYGSDKPDLRVTLEIVDVGDVMRDVAFKVFATPANDPKGRV
AALRIPGGATLSRSEIDGYTEFVKIYGAKGLAYIKVNDVGQLNEAGLQSPIVKNLSEAALHAVMERTGAQNGDLIFFGAD
RAKVVNDALGALRLKIGHEKGHVDGRAWAPLWVVDFPMFEYNEDENRWDALHHPFTAPKDGHEDWLATDPGKCLSKAYDM
VLNGWEVGGGSVRIHREKVQEKVFAALNIGEEERREKFGFLLDALQYGAPPHGGLAFGLDRLVTLMAGAESIRDVIAFPK
TQRASCLMTNAPNVVDEKQLRELHIRLRNPIAADKAA

Sequences:

>Translated_597_residues
MRTHYCGAVTAADVGNTVTLCGWAHRRRDHGGVIFIDLRDREGMVQVVVDPDTPAAFKLAEDVRAEFVLKIEGKVRPRPA
GTENPHLPTGMVEVLTLDLDVLNPSLTPPFQIDDETINENIRLQYRYLDLRREPMQKNLKLRYRVSKIMRDYLDTHGFME
VETPMLTRSTPEGARDYLVPSRVHDGMFYALPQSPQLFKQLLMVSGVDRYFQITKCFRDEDLRADRQPEFTQVDLETSFM
GEEAIMGLVEGMIRDMMQKAQGIELPAAFPRMSYAEAMNRYGSDKPDLRVTLEIVDVGDVMRDVAFKVFATPANDPKGRV
AALRIPGGATLSRSEIDGYTEFVKIYGAKGLAYIKVNDVGQLNEAGLQSPIVKNLSEAALHAVMERTGAQNGDLIFFGAD
RAKVVNDALGALRLKIGHEKGHVDGRAWAPLWVVDFPMFEYNEDENRWDALHHPFTAPKDGHEDWLATDPGKCLSKAYDM
VLNGWEVGGGSVRIHREKVQEKVFAALNIGEEERREKFGFLLDALQYGAPPHGGLAFGLDRLVTLMAGAESIRDVIAFPK
TQRASCLMTNAPNVVDEKQLRELHIRLRNPIAADKAA
>Mature_597_residues
MRTHYCGAVTAADVGNTVTLCGWAHRRRDHGGVIFIDLRDREGMVQVVVDPDTPAAFKLAEDVRAEFVLKIEGKVRPRPA
GTENPHLPTGMVEVLTLDLDVLNPSLTPPFQIDDETINENIRLQYRYLDLRREPMQKNLKLRYRVSKIMRDYLDTHGFME
VETPMLTRSTPEGARDYLVPSRVHDGMFYALPQSPQLFKQLLMVSGVDRYFQITKCFRDEDLRADRQPEFTQVDLETSFM
GEEAIMGLVEGMIRDMMQKAQGIELPAAFPRMSYAEAMNRYGSDKPDLRVTLEIVDVGDVMRDVAFKVFATPANDPKGRV
AALRIPGGATLSRSEIDGYTEFVKIYGAKGLAYIKVNDVGQLNEAGLQSPIVKNLSEAALHAVMERTGAQNGDLIFFGAD
RAKVVNDALGALRLKIGHEKGHVDGRAWAPLWVVDFPMFEYNEDENRWDALHHPFTAPKDGHEDWLATDPGKCLSKAYDM
VLNGWEVGGGSVRIHREKVQEKVFAALNIGEEERREKFGFLLDALQYGAPPHGGLAFGLDRLVTLMAGAESIRDVIAFPK
TQRASCLMTNAPNVVDEKQLRELHIRLRNPIAADKAA

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI40789249, Length=602, Percent_Identity=41.8604651162791, Blast_Score=447, Evalue=1e-125,
Organism=Homo sapiens, GI45439306, Length=237, Percent_Identity=27.8481012658228, Blast_Score=88, Evalue=3e-17,
Organism=Homo sapiens, GI194272210, Length=254, Percent_Identity=24.8031496062992, Blast_Score=83, Evalue=6e-16,
Organism=Homo sapiens, GI5031815, Length=254, Percent_Identity=24.8031496062992, Blast_Score=83, Evalue=8e-16,
Organism=Homo sapiens, GI4758762, Length=289, Percent_Identity=25.6055363321799, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1788173, Length=588, Percent_Identity=57.8231292517007, Blast_Score=701, Evalue=0.0,
Organism=Escherichia coli, GI1789256, Length=285, Percent_Identity=26.6666666666667, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1790571, Length=286, Percent_Identity=26.5734265734266, Blast_Score=80, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI32566633, Length=606, Percent_Identity=35.3135313531353, Blast_Score=330, Evalue=1e-90,
Organism=Caenorhabditis elegans, GI71994340, Length=255, Percent_Identity=24.7058823529412, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17535925, Length=255, Percent_Identity=24.7058823529412, Blast_Score=84, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17535927, Length=255, Percent_Identity=24.7058823529412, Blast_Score=84, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71984122, Length=282, Percent_Identity=25.531914893617, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6325153, Length=635, Percent_Identity=34.9606299212598, Blast_Score=304, Evalue=2e-83,
Organism=Saccharomyces cerevisiae, GI6324256, Length=271, Percent_Identity=25.830258302583, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323011, Length=266, Percent_Identity=26.3157894736842, Blast_Score=74, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6321807, Length=266, Percent_Identity=27.0676691729323, Blast_Score=74, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6320242, Length=259, Percent_Identity=25.8687258687259, Blast_Score=67, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24584738, Length=602, Percent_Identity=39.3687707641196, Blast_Score=371, Evalue=1e-103,
Organism=Drosophila melanogaster, GI17136276, Length=237, Percent_Identity=27.4261603375527, Blast_Score=89, Evalue=8e-18,
Organism=Drosophila melanogaster, GI24640851, Length=251, Percent_Identity=24.7011952191235, Blast_Score=79, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24640849, Length=251, Percent_Identity=24.7011952191235, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19921528, Length=235, Percent_Identity=27.2340425531915, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYD_THIDA (Q3SLL2)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_314200.1
- ProteinModelPortal:   Q3SLL2
- SMR:   Q3SLL2
- STRING:   Q3SLL2
- GeneID:   3671975
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_0442
- NMPDR:   fig|292415.3.peg.1013
- eggNOG:   COG0173
- HOGENOM:   HBG396032
- OMA:   AFPKTQQ
- PhylomeDB:   Q3SLL2
- ProtClustDB:   PRK00476
- BioCyc:   TDEN292415:TBD_0442-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00044_B
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF5
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00459

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 66893; Mature: 66893

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTHYCGAVTAADVGNTVTLCGWAHRRRDHGGVIFIDLRDREGMVQVVVDPDTPAAFKLA
CCCCCCCCEECCCCCCEEEEECHHHHHCCCCCEEEEEEECCCCEEEEEECCCCCHHHHHH
EDVRAEFVLKIEGKVRPRPAGTENPHLPTGMVEVLTLDLDVLNPSLTPPFQIDDETINEN
HHCCEEEEEEECCCCCCCCCCCCCCCCCCCHHHHEEEEHHHCCCCCCCCEECCHHHCCCC
IRLQYRYLDLRREPMQKNLKLRYRVSKIMRDYLDTHGFMEVETPMLTRSTPEGARDYLVP
EEEEEEEEHHHHCHHHCCCHHHHHHHHHHHHHHHCCCCEEECCCCEECCCCCCCHHCCCC
SRVHDGMFYALPQSPQLFKQLLMVSGVDRYFQITKCFRDEDLRADRQPEFTQVDLETSFM
CHHCCCEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCEEEECHHHHC
GEEAIMGLVEGMIRDMMQKAQGIELPAAFPRMSYAEAMNRYGSDKPDLRVTLEIVDVGDV
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEEEEECHHHH
MRDVAFKVFATPANDPKGRVAALRIPGGATLSRSEIDGYTEFVKIYGAKGLAYIKVNDVG
HHHHHHEEEECCCCCCCCCEEEEECCCCCCCCHHHCCHHHHHHHHCCCCCEEEEEECCCC
QLNEAGLQSPIVKNLSEAALHAVMERTGAQNGDLIFFGADRAKVVNDALGALRLKIGHEK
CCCHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHEEEEECCCC
GHVDGRAWAPLWVVDFPMFEYNEDENRWDALHHPFTAPKDGHEDWLATDPGKCLSKAYDM
CCCCCCEECCEEEEECCCEECCCCCCHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
VLNGWEVGGGSVRIHREKVQEKVFAALNIGEEERREKFGFLLDALQYGAPPHGGLAFGLD
HHCCEEECCCEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
RLVTLMAGAESIRDVIAFPKTQRASCLMTNAPNVVDEKQLRELHIRLRNPIAADKAA
HHHHHHHCHHHHHHHHCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MRTHYCGAVTAADVGNTVTLCGWAHRRRDHGGVIFIDLRDREGMVQVVVDPDTPAAFKLA
CCCCCCCCEECCCCCCEEEEECHHHHHCCCCCEEEEEEECCCCEEEEEECCCCCHHHHHH
EDVRAEFVLKIEGKVRPRPAGTENPHLPTGMVEVLTLDLDVLNPSLTPPFQIDDETINEN
HHCCEEEEEEECCCCCCCCCCCCCCCCCCCHHHHEEEEHHHCCCCCCCCEECCHHHCCCC
IRLQYRYLDLRREPMQKNLKLRYRVSKIMRDYLDTHGFMEVETPMLTRSTPEGARDYLVP
EEEEEEEEHHHHCHHHCCCHHHHHHHHHHHHHHHCCCCEEECCCCEECCCCCCCHHCCCC
SRVHDGMFYALPQSPQLFKQLLMVSGVDRYFQITKCFRDEDLRADRQPEFTQVDLETSFM
CHHCCCEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCEEEECHHHHC
GEEAIMGLVEGMIRDMMQKAQGIELPAAFPRMSYAEAMNRYGSDKPDLRVTLEIVDVGDV
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEEEEECHHHH
MRDVAFKVFATPANDPKGRVAALRIPGGATLSRSEIDGYTEFVKIYGAKGLAYIKVNDVG
HHHHHHEEEECCCCCCCCCEEEEECCCCCCCCHHHCCHHHHHHHHCCCCCEEEEEECCCC
QLNEAGLQSPIVKNLSEAALHAVMERTGAQNGDLIFFGADRAKVVNDALGALRLKIGHEK
CCCHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHEEEEECCCC
GHVDGRAWAPLWVVDFPMFEYNEDENRWDALHHPFTAPKDGHEDWLATDPGKCLSKAYDM
CCCCCCEECCEEEEECCCEECCCCCCHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
VLNGWEVGGGSVRIHREKVQEKVFAALNIGEEERREKFGFLLDALQYGAPPHGGLAFGLD
HHCCEEECCCEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
RLVTLMAGAESIRDVIAFPKTQRASCLMTNAPNVVDEKQLRELHIRLRNPIAADKAA
HHHHHHHCHHHHHHHHCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA